1.Discovery of topAp4 as a new sensing element for TNT detection
Naipeng KAN ; Junjie TAN ; Wei WANG ; Jingyi LING ; Guolong QU ; Yu SHAO ; Jing JIN ; Gang LIU ; Huipeng CHEN
Military Medical Sciences 2015;(7):523-527
Objective To screen the sensing elements for TNT detection in Escherichia coli genome.Methods A genome promoter library with cutting E.coli K-12 MG1655 genome was constructed.Bacterial luciferase luxCDABE was used as a reporter gene during promoter screening.We discovered TNT sensing elements through several rounds of screen-ing.Through analysis of sensitivity, specificity and timeliness, the promoter activity of the elements was evaluated,and the functional sequence of the elements was further confirmed.Results and Conclusion We successfully constructed an E.co-li K-12 MG1655 genome library , from which a TNT sensing element was discovered,which had a good performance in the analysis of sensitivity, specificity and timeliness.In this study, we reported that the topAp4 is a TNT sensing element for the first time.We also verified its excellent promoter activity.
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