Objective To study the chemotactic activity of T lymphocytes from patients with psoriasis to proinflammatory cytokines in vitro and its role in the pathogenesis in psoriasis. Methods A 48 microchemotaxis chamber was employed to determine T cell chemotactic activity. In addition, the expression of T cell activation markers such as HLA DR and interleukin 2 receptor was analysed with fluorescence activated cell sorting technique and serum IL 8 level was measured with ELISA. 45 patients with psoriasis (23 patients with severe psoriasis and 22 patients with mild psoriasis) and 21 patients with atopic dermatitis were investigated, and 20 healthy controls were tested equally. Results ①T cell chemotactic responses were significantly decreased in patients with severe psoriasis and atopic dermatitis as compared to healthy controls. ②Increased expression of activation markers such as HLA DR and interleukin 2 receptor was demonstrated in circulating T cells from severe psoriatic patients and atopic dermatitis patients in comparison to healthy controls. ③Serum IL 8 level was significantly increased in patients with psoriasis and atopic dermatitis. Conclusion The in vitro chemotactic response of circulating T cells in patients with severe psoriasis to IL 8 is significantly impaired, furthermore, the in vivo activation state of T lymphocytes in these patients and increased level of serum IL 8 seem to be associated to the decreased in vitro T cell chemotactic response.

Objective To study the relationship between the in situ expression of infiltrating T lymphocyte subsets and leukocyte adhesion molecules in psoriatic patients. Methods Immunohistochemical technique was employed to study the expression of infiltrating T lymphocyte subsets (CD3, CD4, CD8) and leukocyte adhesion molecules (ICAM 1, ELAM 1, VCAM 1) in the lesional and non lesional psoriatic skin. Results There was a significant increase of T lymphocyte subsets (CD3, CD4 and CD8) and adhesion molecules (ICAM 1, ELAM 1, VCAM 1) in the lesional skin than those in non lesional skin in psoriasis, and that there is a significant positive correlation between the density of infiltrating T lymphocyte subsets and the intensity of in situ expression of adhesion molecules. In comparison to the materials from normal controls, the upregulation expression of adhesion molecules was observed in non lesional skin and normal appearance skin after short duration topical treatment with corticosteroid. Conclusion Increased upregulation expression of adhesion molecules is closely associated with the infiltration of T lymphocytes in lesional skin of psoriasis and may be one of the factors for the recurrence of psoriasis.

Objective To address the question whether circulating T cells from the patients with primary and metastatic malignant melanoma show altered chemotaxis to monocyte chemotactic protein 1 (MCP 1) and its relation to tumor infiltrating lymphocyte (TIL) and metastasis. Methods Chemotactic responsiveness of T cells towards MCP 1 and immuno histochemistry study were investigated in patients with primary and metastatic melanoma compared to patients with basal cell carcinoma and healthy persons. Results T cells from primary and metastatic melanoma patients showed a significantly decreased chemotactic migration towards MCP 1 and that T cells from patients with basal cell carcinoma showed normal chemotactic response. Immuno histochemistry study showed that there was no correlation between the density of TIL and the decreased chemotaxis of circulating T cells to MCP 1 in patients with primary melanoma. Conclusion Circulating T cells from patients with primary and metastatic malignant melanoma show a MCP 1 specific decrease in chemotactic migration, this may be due to abnormal expression or modulation of MCP 1 receptor on these cells.

Objective To investigate the relationship between keratinocyte growth factor (KGF), nerve growth factor(NGF)and psoriasis;and to study the interaction between keratinocytes and interstitial cells in psoriasis. Methods The expression of KGF, KGF receptor (KGFR), NGF, NGF receptor (NGFR), epidermal growth factor receptor (EGFR) and proliferating cell neuclear antigen (PCNA) was studied with immunohistochemical technique (SP) in lesional skin, non lesional skin and normal controls. Results Significant overexpression of KGF and KGFR was present in psoriatic basal cell layers and suprabasal cell layers compared to that in non lesional and normal controls (P0.05). Expression of KGF and KGFR was absent in dermis. Expression of NGF and NGFR was observed mainly in granular cell layers and upper spinous layers, and was significantly different between normal controls and non lesional, between non lesional and lesional skin (P

Objective:To explore the protective effect of ischemic postconditioning on myocardium during emergency percutaneous coronary intervention (PCI) in patients with ST segment elevation myocardial infarction (STEMI) . Methods:According to different treatment method ,a total of 82 STEMI patients ,who hospitalized in the depart-ment of Cardiology of Affiliated Hospital of Guangxi Medical University from Jan 2011 to Dec 2012 and received PCI within 12h ,were divided into ischemic postconditioning group (n= 42) and pure reperfusion group (n= 40) . Heart function etc .were evaluated in both groups .Results:Compared with pure reperfusion group ,there were sig-nificant rise in complete ST segment resolution rate (55.0% vs .81.0% ) and left ventricular ejection fraction [ (0.5 ± 0.1) vs .(0.7 ± 0.1)] ,significant reductions in arrhythmia rate (60.0% vs .21.4% ) ,wall motion score index [WMSI ,(2.0 ± 0.7) scores vs .(1.3 ± 0.6) scores] ,myocardial infarction size [MIS ,(15.1 ± 7.1)% vs .(9.9 ± 5.3)% ] ,radionuclide myocardial perfusion defect extent score [ES ,(0.4 ± 0.1)% vs .(0.2 ± 0.1)% ] and severity score [SS ,(2.3 ± 1.1)% vs .(1.8 ± 1.2)% ] in ischemic postconditioning group ,P<0.05~ <0.01 .Conclusion:Is-chemic postconditioning can significantly improve extent of myocardial ischemia reperfusion injury and protect myo-cardial tissues in STEMI patients .

Objective To investigate the correlation between expression of four adhesion molecules in sera and in skin lesions of patients with psoriasis vulgaris, as well as their relevance to clinical severity of the disease. Methods Enzyme-linked immunosorbent assay (ELISA) was used to determine the serum levels of ICAM-1, ICAM-3, VCAM-1 and ELAM-1 in 36 patients with plaque type psoriasis vulgaris and 36 healthy controls. Avidin biotin immunoperoxidase staining system was employed to quantitate expression of the four adhesion molecules in psoriatic skin lesions before and after treatment. Results Significantly increased expression of four adhesion molecules was found in psoriatic skin lesions (P

Objective To study the hemostnsis effects of compression cords oppressing after femoral artery puncture and encheiresis. Methods Self-made compression cords were used to stop bleeding of femoral artery puncture sites in 2164 patients underwent 3046 cases of arteriopuncture encheiresis. The total eases were divided into group A (1482 eases) and group B (1564 cases) according to different treatment of oppression and breaking duration which were 8-10 hours for group A and 5-6 hours for group B, the hemostasis effects, discomfortableness and related complications within two groups were evaluated. Results The proportion of patients who got good hemostasis effects were 99.2% in group A, 99.0% in group B. There was no difference in hemostasis effects between two groups (P> 0.05). While the rate of discomfortableness in group A (63.8%) was higher than that in group B(42.9%) (P<0.05). The related serious complications were 2 patients in group A, but no occurred in group B. Conclusion Self-made compression cords op- pressing and 5-6 hours breaking time course can achieve both finer hemostasis effect and lower frequency of complications after femoral artery puncture and encheiresis.

Objective:To establish a method to detect food allergen based on Latex-enhanced immune turbidimetry ( PETIA) and apply to clinic.Methods: The PETIA method was used for evaluating the detection system including standard curve , detection-limit,stability, intra-assay and inter-assay precision and cross-reactivity,clinical normal serum specimens and clinical allergic diseases serum specimens were measured to evaluated the perspective of the clinical application .Results: The standard curve :Y=837.1 x2-125.2x+10.036, linear range: 0.0-400 U/ml, the correlation coefficient of the standard curve was 0.996, the intrabatch and interbatch precision was<10%.The normal reference range was≤33.5 U/ml,the AUC of ROC curve was 0.957,the sensitivity was 89.61%,The specificity was 65.22%, the accuracy was 84.00%, and the positive predicted value was 89.61%, the negative predicted value was 65.22%.the test results have strong correlation with ELISA ( r=0.890 2 ) .Conclusion: The PETIA method for detecting food allergen achieved corresponding clinical application standards and may be used for the diagnosis of food allergies .

Objective To detect and explore the expression of ARD1 and its clinical significance in the nasopharyngeal in-flammatory tissue ,nasopharyngeal carcinoma group and its subgroups .Methods Expression of ARD1 in nasopharyngeal carcinoma (56 cases) and nasopharyngeal inflammatory tissue (20 cases) were detected by immunohistochemical staining SP ,the correlation between the expression of ARD1 and age ,gender ,histological grade ,TNM clinical stage and tumor metastasis were analysed . Results The positive expression rate of ARD1 were 10 .00% (2/20) ,55 .35% (31/56) in the nasopharyngeal inflammatory tissue and nasopharyngeal carcinoma ,respectively .The expression level of ARD1 in nasopharyngeal carcinoma was significantly higher than in the nasopharyngeal inflammatory tissue ,the difference was significant (P< 0 .05) ;expression of ARD1 in the nasopharynge-al carcinoma was correlated with the histological grade of nasopharyngeal carcinoma(P< 0 .05) and the expression was increased in poor differenciation tissue .But there was no statistical difference between the expression of ARD1 and the patient′s age ,gender , TNM clinical stage ,tumor metastasis(P> 0 .05) .Conclusion The expression of ARD1 is high in nasopharyngeal carcinoma ,and has a closely correlation with diffferentiation level of tumor ,which suggested that ARD1 may be involved in the the occurrence and development of nasopharyngeal carcinoma .However ,further research needs to be done for its mechanism in the nasopharyngeal car-cinoma .

OBJECTIVE To investigate thechanges and clinical significance of serum Nesfatin-1 in patients with obstructive sleep apnea hypopnea syndrome(OSAHS) and type 2 diabetes mellitus (T2DM).METHODS 25 OSAHS cases who visited the department of otolaryngology, pneumology department, endocrinology department, physical examination center and sleep monitoring room from December 2014 to April 2015 were assigned as OSAHS group, and 25 patients with OSAHS and T2DM as OSAHS combination T2DM group, and 25 other patients as control group. All patient were took polysomnography, and the height, waist, neck circumference and BMI with all subjects were recorded. The serum Nesfatin-1 and fast blood glucose levels of all patients were measured.RESULTS The gender, age, height, waist and neck circumference had no statistical difference among all groups. The height and BMI in OSAHS group and OSAHS combination T2DM group were statistically significant difference compared with the control group (P<0.05); the FBG in OSAHS combination T2DM group and OSAHS group were significantly higher than that of the control group, and the FBG in OSAHS combination T2DM group were significantly higher than that of the OSAHS group, with statistically significant(P<0.05); The AHI in OSAHS combination T2DM group were significantly higher than that of the OSAHS group, with statistically significant(P<0.05); the serum Nesfatin-1 in OSAHS combination T2DM group and OSAHS group were significantly higher than that of the control group, with statistically significant(P<0.05); FBG, AHI and Nesfatin-1 were positively correlated with each other.CONCLUSION The serum Nesfatin-1 in patients with OSAHS combination T2DM are in a high level.