ObjectiveTo explore the curative effect of Qufengqingre method in treatment of the patients with cough after cold.Methods74 patients with cough after cold were selected and randomly divided into the control group(36 cases) and the observation group (38 cases ).The control group was given roxithromycin (150 mg every time,two times every day),ketotifen( 1 mg every time,one time every night),pentoxyverine citrate(50mg every time,3 times every day).The observation group was given Qufengqingre decoction,and the decoction was boiled in water twice per dose,and mixed juice about 300 ml was abtained for morning and evening oralling.The efficacy,symptoms score and adverse reactions were observed.ResultsIn the control group,7 patients were cured,effective in 21 cases,invalid in 8 cases,and in the observation group,17 cases were cured,effective in 18 cases,invalid in 3 cases.The total effective rate of the observation group was 92.1％ higher than 77.8 ％ of the control group ( x2 =4.16,P ＜ 0.05 ).The cough and sputum score of the two groups before treatment was ( 2.18 ± 0.73 ) points and ( 1.09 ± 0.27 ) points,(2.26 ± 0.65 ) points and ( 1.12 ± 0.23 ) points,respectively,and after treatment was ( 1.11 ± 0.37 ) points and (0.65 ± 0.16) points,(0.72 ± 0.19 ) points and (0.35 ± 0.08 ) points.Differences in scores between the two groups before treatment were not statistically significant(t =1.29,1.31,all P ＞ 0.05 ) ; In the two groups before and after treatment were statistically significant( t =2.68,2.59,2.43,2.41,all P ＜ 0.05 ) ; After treatment the difference between the two groups were statistically significant( t =2.53,2.37,all P ＜ 0.05 ).Both groups had no obviously adverse reactions.ConclusionQufengqingre method in treatment of the patients with cough after cold had remarkable effect.

Objective To explore the clinical features,laboratory tests,imaging characteristics of neurobrucellosis,and to improve the understanding of the disease.Methods A retrospective analysis was performed in seven patients with neurobrucellosis in our hospital from January 2002 to May 2013.Results The proportion of men and women was 6∶ 1,and the average age was 46 years.One case was attacked with the symptoms of cerebrovascular disease,another one with the symptoms of myelitis,and the other five with the symptoms of meningoencephalitis.Six had positive results of serum agglutination test,and the other one had a positive result of blood culture instead.Imaging findings lacked of specificity.There were two patients whose lesions were in the hemisphere cortex,subcortical,basal ganglia and other parts,four patients whose lesions were in the frontal lobe,one in the chest pulp,and one in both sides of cerebellum and pons.After systematical treatment,one case died,the other 6 cases recovered,and no one relapsed or got functional disability during the one-year follow-up.Conclusions Neurobrucellosis is a rare type of brucellosis,and its clinical manifestations lack of specificity,and imaging and serological detections are important for diagnosis.It is beneficial for patients if diagnosed early and treated systematically,so good understanding of this disease,early diagnosis and treatment are of great value.

This paper underlines the composition of OS(Quorum Sensing) systems in Pesudomonas aeruginosa,the resistance mechanism of Pesudomonas aeruginosa and the relevance between QS systems and the resistance mechanism.What's more,it introduces several pathways of the interfering of QS systems to weaken the resistance.These will show a new prospect to the control of bacterial infection.

Triple-negative breast cancer(TNBC)is a special subtype of breast cancer which is invalid to endocrine therapy.Anti-Her2 targeted therapies such as herceptin and lapatinib are not suitable to TNBC.At present,conventional chemotherapy is the only way for the medical therapy of TNBC.Thus,searching for novel therapeutic agents for TNBC is one of hot researches of breast cancer.New targeted therapy drugs such as PARP-1 inhibitors,EGFR inhibitors,CXCR4 inhibitors,anti-angiogenesis drugs,Src tyrosine kinase inhibitor,and mTOR inhibitor are being researched.

As the investments from the state and society in universities increased rapidly, the scientific and technological resources gained huge promotion. However, the efficiency of some instruments remained low, and the repeated purchases of some instruments occurred frequently. Here the authors discussed about building a practical, reasonable and efficient system for lab management.

Among the different x-ray features of breast carcinoma, mass and calcification are the most significant reference parameters in the screening and diagnosis of early carcinoma. Mammographic follow-up after conservation therapy is the other important value of mammography for breast carcinoma. Calcification is the most important sign to show residue or recurrence of conservation therapy.

In order to construct a recombinant plasmid containing short hairpin RNA (shRNA) targeting survivin and to investigate its effect on survivin expression and cell apoptosis of human retinoblastoma cell line Hxo-rb44 in vitro, RNA interference plasmid pSIRENS that can express shRNA of survivin was designed, constructed, and transfected into human retinoblastoma cell line Hxo-rb44. Survivin and c-Myc expression was detected by reverse transcription polymerase chain reaction (RT-PCR) and Western blot. Apoptosis of Hxo-rb44 cells was assayed by Honchest33258 staining and cell growth curve was drawn. The results showed that the oligonucleotide targeting survivin was identified in pSIRENS plasmid. After pSIRENS plasmid transfected, survivin and c-Myc expression in Hxo-rb44 cells was decreased significantly. Apoptotic rate of cells was up-regulated from (3.5+/-1.29) % to (36.1+/-19.66) %. The proliferation ability of Hxo-rb44 cells was inhibited. No significant effects on survivin expression and apoptosis of the cells were found when negative control plasmid was transfected. In conclusion, the plasmid containing shRNA targeting survivin was constructed successfully. It could inhibit efficiently the expression of survivin and c-Myc in human retinoblastoma cell Hxo-rb44 in vitro. The inhibition of the expression of c-Myc might be involved in the apoptosis of Hxo-rb44 cells.

ObjectiveThis article investigates the distribution of psychological models and its association with asthma by means of examining the psychological models of asthmatic patients.MethodsUsing 80.8 test table provided by the Institute of Applied Psychology of Suzhou University to exam the psychological models of two hundred cases in their non- acute episodes. Results From two hundred asthmatic patients we can notice the association between psychological models and the degree of asthma. In severe asthmatic patients, most was dispersed - puzzle model, occupying 65.3% (17/26),the second was intermediate model 30.7% (8/26). The majority in medium asthmatic patients was intermediate model, taking 53% (53/100), the second was dispersed-puzzle model, 34% (34/100).The easy-disturbance model was the most in mild ones, 51.4% (38/74), the intermediate model was the second, 25.6%(19/74). ConclusionThere are different psychological models in asthmatic patients. In treatments, except the medical therapy, we should direct and take active care of the patients in psychological health aspect.

Objective To explore the expression of EZH2 in clear cell carcinoma and its significance.Methods EZH2 protein expression was assessed by immunohistochemical method in tissue specimens of normal nephridium and clear cell carcinoma.Results The rate of EZH2 protein expression was 12.5% and 74.1%(P

Objective To investigate the relationship between hepatitis B virus pre-core region 1896 site mutation in cases of chronic hepatitis B and IFN-alpha treatment.Methods 200 patients with chronic hepatitis B were randomly divided into experimental group(n=100) and control group(n=100).The patients in experimental group were treated with regular liver-protective drugs and IFN-alpha,while Control group patients received only regular liver-protective drugs for 6 months.PCR-RFLP were used to detect hepatitis B virus(HBV)DNA with pre-C region 1896 site mutation in two grops pre and post treatment.Results The mutation rates before interferon treatment were 20%(20/100) and 18%(18/100) in experimental group and control group,respectly.After treatment,the effective rates in experimental group were 60%(12/20) and 50%(40/80) in Patients with Chronic Hepatitis B variant and wild strain virus Infection,respectly.The effective rate in experimental group was significantly higher than that in control group.Conclusion IFN-alpha therapy was not correlated with genomic variability of the core region.