The morphometry of the intramembranous particles (IMP) of the dog sinus node cells was studied with freeze-cleaved specimens under H-500 electron microscope. The photographs of IMP of the P cells and transitional cells were analysed with imaging analytical system (IBAS Ⅰ+Ⅱ type). The results are as follows. The mean area of IMP: on PF and EF faces of P cells are 25.800nm~2 and 17.816nm~2. The mean values of IMP on PF and EF faces of the transitional cells are 46.041nm~2 and 88.633nm~2. The averages of the perimeter of IMP on the PF and EF faces of P cells are 20.735 nm and 18.078 nm, respectively. And those of the transitional cells are 28.780 nm and 43.901 nm. The diameters of circles of IMP of PF and EF faces of P cells are 5.220 nm and 4.402 nm. Those of PF and EF faces of the transitional cells are 7.010 nm and 10.476 nm. The mean values of form factor of IMP on PF and EF faces of P cells are 0.653 and 0.643, and those of transitional cells are 0.627 and 0.573. The mumbers of IMP are 1325/?m~2 and 345/?m~2 on PF and EF faces of P cells, and the mumbers of IMP of the transitional cells are 3225/?m~2 on PF face and 590/?m~2 on EF face.

In order to improve works of helping this special group,medical students' current ideological and political situation and characteristics are analyzed.Then applying the Satir family therapy into the medical students' ideological and political education process and analyzing the applicability of Satir into the ideological and political work from the three views of system,family values and humanity.Family tree technology,home remodeling,empty chair technique and iceberg metaphor technology of Satir family therapy are analyzed in details in ideological and political education of medical students with learning difficulties through some helping cases.the three views of system,family values and humanity of Satir family therapy have some value in helping students,building positive value instead of their negative mind towards learning.With the promotion of Satir family's concept and technology,it also helps the ideological and political educators have deep thoughts about the education methods.

Three nuclear type segeragants were isolated from a heterokaryon of Fusarium oxysporum f.sp.vasinfectum strain Ag149.Their cultural character and pathogenicity were different.The differences of their GC content for total DNA and nuclear DNA were between 0 1% to 0 4% and 0 4% to 0 8% respectively,as measured by thermal denaturation method.The 18S rDNA partial sequences of 1477 base pairs in these segregants were identical.

In order to elucidate the ultrastructural changes of human spermatozoa afteradministration of gossypol acetate 40 mg/day for 50 days,freeze-replica techniqueswere employed.In addition to the ordinary changes of spermatozoa,such as swelling,wasting,atrophy,tortuosity,nodulation in the head and/or tail region and fracture betweenhead and tail,the internal structure,biomembrane and intramembranous particles ofsperm also showed relatively more sensitive changes.The changes of biomembranerevealed certain shift in regard to the firmness of reciprocating attachment of inner-and outer-leaflets of the unit membrane.On the other hand,the changes of intra-membranous particles revealed a specialized arrangement,this is,on the plasmamembrane of head and tail they were concentrated into lattice work,whereas,in thehind-head region they accumulated as clusters.In the hind-acrosomal membranetwo types of particles,large and small,may be distinguished,the smaller ones werearranged orderly in lines of all directions.As for the nuclear pores,no changewas found in this study.It is considered that the influences of gossypol upon human spermatozoa,firstof all,were shown in the biomembrane and intramembranous particles,and theordinary changes in appearance were the secondary result.In the discussion,it'ssuggested that a special agent,gossypol receptor,responsible for the gossypol effectsto the sperm,may be present in the plasma membrane of the spermatozoa.

Objective To establish an effective qualitative discrimination method for Selaginella medicinal materials.Meth- ods Thin layer chromatography(TLC)method was used.Results The TLC method has a good specialization for identifying Selaginella medicinal materials and can distinguish Selaginella rnoellendorfii from other 10 familiar species in northern areas of Guangdong province.Conclusion The method can help to control the quality of Selaginella moellendorfii Tablet.

Objective To observe the clinical efficacy of point injection at Xialiao point (BL 34) for levator ani syndrome. Methods A hundred levator ani syndrome patients were randomly allocated to a treatment group and a control group, 50 cases each. The treatment group received point injection at Xialiao point (BL 34) and the control group was intervened by biofeedback therapy. The Visual Analogue Scale (VAS), component scores of the MOS 36-item Short-From Health Survey (SF-36), anal resting and squeeze pressures were recorded in the two groups before and after the treatment. The therapeutic effects and therapy costs were compared between the two groups.Results The VAS, component scores of SF-36, anal resting and squeeze pressures were significantly changed after intervention in the two groups (P<0.05). After treatment, the VAS, a part of the SF-36 score [physiological function (PF), body pain (BP), vitality (VT) and social function (SF) scores], anal resting and squeeze pressures in the treatment group were significantly different from that in the control group (P<0.05). The total efficacy rate was 88.0% in the treatment group versus 80.0% in the control group, and the difference was statistically significant (P<0.05). The therapy costs in the treatment group were (327.31±13.42) RMB and (408.45±21.56) RMB in the control group, and the difference was statistically significant (P<0.01).Conclusions Point injection at Xialiao point (BL 34) is an effective method for levator ani syndrome.

Objective To summarize the experience in diagnosis and treatment of popliteal vein entrapment syndrome (PVES). Methods A total of 57 patients with PVES were selected from the Department of Vascular Surgery in Xinxiang Central Hospital from March 2009 to October 2015. Of which 43 patients were severe stenosis of popliteal vein (stenosis degree>90%), and another 14 cases were with stenosis less than 90%. All the patients underwent ascending venography of low limb to confirm the clinical classification after admission. Forty-three cases with severe stenosis of the popliteal vein were treated with releasing popliteal vein of entrapment and stripping varicose veins. The static pressure and dynamic pressure of popliteal vein and foot dorsal vein were measured before and after operation. Another 14 patients were treated with medical circulation driven sock and medical therapy. Results The degrees of popliteal vein stenosis were more than 75% in all patients. The patients were divided into bove-knee stenosis (n=9), knee stenosis (n=18), and below-knee stenosis (n=30) according to the different parts of stenosis. Forty-three patients treated with surgery showed relief of leg swelling and pain, and ulcer healing. And the imaging examination showed that there were no obvious compression and stenosis of popliteal vein, and vascular filling was well. The static pressure and dynamic pressure of the popliteal vein and dorsal vein were lower than those before surgery (P<0.05). The lower limb swelling and pain were relieved, and varicose veins of lower limbs were no longer continued to increase in 14 patients with conservative treatment. Conclusion PVES is easy to be misdiagnosed, which should be paid attention to, and satisfactory clinical results can be achieved by releasing popliteal vein of compression combined with stripping varicose veins in patients with serious symptoms .

Observation had been made on two male adults' hearts.Under electron microscopy,it is found that the A-V node consists of three types of cells,i.e.P cells,transitional cells and Purkinje cells.Because the specimen was cut from the central part of the A-V node, therefore the general myocardial cells were not observed.One of characteristics distingui- shed from the sino-atrial node is the presence of a few Purkinje cells in the A-V node.The number of P cells is far less than S-A node,and the transitional cells are predominant.In the interstitial tissues of the A-V node,the collagenous fibers are not so much as in S-A node but a numbers of capillarie,fibroblasts,mast cells and non-medul- lated nerve fibers can be seen.

Twenty adult male rats, weighing 200～300 g were divided into 3 groups: (1) Six. animals were stimulated through two electrodes fixed on the tail to produce somatic pain.(2) Six animals were stimulated by increasing pressure in the stomach through an air balloon to cause visceral pain. And 8 animals served as control.All the animals were sacrificed and their neurohypophysis were fixed in 4％ glutaraldehyde and 1％ OsO_4 successively. Their section were cut with an LKB microtome and observed under H 500 electron microscope.The experimental results summarized as follows:1. The bodies of pituicytes under the pain stimulation were hypertrophied. The rough endoplasmic reticulum, polyribosomes and mitochondria were increased. The Golgi complex was well developed. There were numerous elliptical vesicles appearing in the cytoplasm of pituicytes.2. The number of large lipid masses was increased while the processes of pituicytes engulfed the degenerative neurosecretory terminal into them and the pituicyte digted it finally.3. Neurosecretory granules were decreased but the synaptic small vesicles were increased in the dilated parts of neurosecretory fibers after the pain stimulation.4. The continuous network of perivascular space and interstitial space between the dilated parts of neurosecretory fibers were widened.

Pain just tolerable was caused intermittently by electric stimulation on rat's tail or by distented baloon in stomach for 30 min. The changes of intermediate lobe are summarized as follows: (1) The cell body, nucleus or nucleolus was hypertrophied. The number of rough endoplasmic reticulum, particularly of its vesicular cisternae was increased. Golgi complex enlarged. The ribosome multiplied. All of these change indicated that the activity of cells was increased and the rate of synthesis enhanced. (2) Both the secretory granules shifted to the periphery of cell body and some of them showed pale density. These changes were interpreted as increase of seretory activity. (3) The mitochondria became enlarged and the crsitae in them were changed into tubular form. This suggested an acceleration of cell metabolism, which might provide a large amount of energy. (4) There was no degenerative change. (5) The cytological changes caused by visceral pain were simillar with, but more striking than, those caused by somatic pain. (6) The secretions were increased, among which ?-endorphin and ACTH etc were known to be closely related to analgesia.