The morphometry of the intramembranous particles (IMP) of the dog sinus node cells was studied with freeze-cleaved specimens under H-500 electron microscope. The photographs of IMP of the P cells and transitional cells were analysed with imaging analytical system (IBAS Ⅰ+Ⅱ type). The results are as follows. The mean area of IMP: on PF and EF faces of P cells are 25.800nm~2 and 17.816nm~2. The mean values of IMP on PF and EF faces of the transitional cells are 46.041nm~2 and 88.633nm~2. The averages of the perimeter of IMP on the PF and EF faces of P cells are 20.735 nm and 18.078 nm, respectively. And those of the transitional cells are 28.780 nm and 43.901 nm. The diameters of circles of IMP of PF and EF faces of P cells are 5.220 nm and 4.402 nm. Those of PF and EF faces of the transitional cells are 7.010 nm and 10.476 nm. The mean values of form factor of IMP on PF and EF faces of P cells are 0.653 and 0.643, and those of transitional cells are 0.627 and 0.573. The mumbers of IMP are 1325/?m~2 and 345/?m~2 on PF and EF faces of P cells, and the mumbers of IMP of the transitional cells are 3225/?m~2 on PF face and 590/?m~2 on EF face.

In order to improve works of helping this special group,medical students' current ideological and political situation and characteristics are analyzed.Then applying the Satir family therapy into the medical students' ideological and political education process and analyzing the applicability of Satir into the ideological and political work from the three views of system,family values and humanity.Family tree technology,home remodeling,empty chair technique and iceberg metaphor technology of Satir family therapy are analyzed in details in ideological and political education of medical students with learning difficulties through some helping cases.the three views of system,family values and humanity of Satir family therapy have some value in helping students,building positive value instead of their negative mind towards learning.With the promotion of Satir family's concept and technology,it also helps the ideological and political educators have deep thoughts about the education methods.

Objective To observe the clinical efficacy of point injection at Xialiao point (BL 34) for levator ani syndrome. Methods A hundred levator ani syndrome patients were randomly allocated to a treatment group and a control group, 50 cases each. The treatment group received point injection at Xialiao point (BL 34) and the control group was intervened by biofeedback therapy. The Visual Analogue Scale (VAS), component scores of the MOS 36-item Short-From Health Survey (SF-36), anal resting and squeeze pressures were recorded in the two groups before and after the treatment. The therapeutic effects and therapy costs were compared between the two groups.Results The VAS, component scores of SF-36, anal resting and squeeze pressures were significantly changed after intervention in the two groups (P<0.05). After treatment, the VAS, a part of the SF-36 score [physiological function (PF), body pain (BP), vitality (VT) and social function (SF) scores], anal resting and squeeze pressures in the treatment group were significantly different from that in the control group (P<0.05). The total efficacy rate was 88.0% in the treatment group versus 80.0% in the control group, and the difference was statistically significant (P<0.05). The therapy costs in the treatment group were (327.31±13.42) RMB and (408.45±21.56) RMB in the control group, and the difference was statistically significant (P<0.01).Conclusions Point injection at Xialiao point (BL 34) is an effective method for levator ani syndrome.

Objective To summarize the experience in diagnosis and treatment of popliteal vein entrapment syndrome (PVES). Methods A total of 57 patients with PVES were selected from the Department of Vascular Surgery in Xinxiang Central Hospital from March 2009 to October 2015. Of which 43 patients were severe stenosis of popliteal vein (stenosis degree>90%), and another 14 cases were with stenosis less than 90%. All the patients underwent ascending venography of low limb to confirm the clinical classification after admission. Forty-three cases with severe stenosis of the popliteal vein were treated with releasing popliteal vein of entrapment and stripping varicose veins. The static pressure and dynamic pressure of popliteal vein and foot dorsal vein were measured before and after operation. Another 14 patients were treated with medical circulation driven sock and medical therapy. Results The degrees of popliteal vein stenosis were more than 75% in all patients. The patients were divided into bove-knee stenosis (n=9), knee stenosis (n=18), and below-knee stenosis (n=30) according to the different parts of stenosis. Forty-three patients treated with surgery showed relief of leg swelling and pain, and ulcer healing. And the imaging examination showed that there were no obvious compression and stenosis of popliteal vein, and vascular filling was well. The static pressure and dynamic pressure of the popliteal vein and dorsal vein were lower than those before surgery (P<0.05). The lower limb swelling and pain were relieved, and varicose veins of lower limbs were no longer continued to increase in 14 patients with conservative treatment. Conclusion PVES is easy to be misdiagnosed, which should be paid attention to, and satisfactory clinical results can be achieved by releasing popliteal vein of compression combined with stripping varicose veins in patients with serious symptoms .

Twenty adult male rats, weighing 200～300 g were divided into 3 groups: (1) Six. animals were stimulated through two electrodes fixed on the tail to produce somatic pain.(2) Six animals were stimulated by increasing pressure in the stomach through an air balloon to cause visceral pain. And 8 animals served as control.All the animals were sacrificed and their neurohypophysis were fixed in 4％ glutaraldehyde and 1％ OsO_4 successively. Their section were cut with an LKB microtome and observed under H 500 electron microscope.The experimental results summarized as follows:1. The bodies of pituicytes under the pain stimulation were hypertrophied. The rough endoplasmic reticulum, polyribosomes and mitochondria were increased. The Golgi complex was well developed. There were numerous elliptical vesicles appearing in the cytoplasm of pituicytes.2. The number of large lipid masses was increased while the processes of pituicytes engulfed the degenerative neurosecretory terminal into them and the pituicyte digted it finally.3. Neurosecretory granules were decreased but the synaptic small vesicles were increased in the dilated parts of neurosecretory fibers after the pain stimulation.4. The continuous network of perivascular space and interstitial space between the dilated parts of neurosecretory fibers were widened.

Observation had been made on two male adults' hearts.Under electron microscopy,it is found that the A-V node consists of three types of cells,i.e.P cells,transitional cells and Purkinje cells.Because the specimen was cut from the central part of the A-V node, therefore the general myocardial cells were not observed.One of characteristics distingui- shed from the sino-atrial node is the presence of a few Purkinje cells in the A-V node.The number of P cells is far less than S-A node,and the transitional cells are predominant.In the interstitial tissues of the A-V node,the collagenous fibers are not so much as in S-A node but a numbers of capillarie,fibroblasts,mast cells and non-medul- lated nerve fibers can be seen.

Aim To establish a new simple, easy and economical PCR method for determining cyp2d6~*10 allele and studying its distributive frequencies in Chinese population. Methods The new PCR are established and compared with the classical PCR-RFLP. The 224 samples have been determined with the new PCR.Results the results of the two methods go on the way. The allele distribution frequencies of cyp2d6~*10 resembles the reports. Conclusion the new method is proved to be accurate and convenient. It provides a genetics basis for the drug therapy with individuals in clinics.

Epidemiological, clinical, laboratory and treatment data, as well as outcomes of 66patients diagnosed as brucellosis during 1984 to 2010 in Peking Union Medical College Hospital were retrospectively analyzed. Thirty-four (51.5%) patients had a history of close contact with sheep or cows infected with brucellosis, eight (12. 1% ) had eaten not-fully-boiled or roasted mutton, three (4. 5% ) had drunk or contacted with contaminated milk, and transmission route was unknown in 21 (31.8%). The most common manifestations were fever ( 97.0%, 64/66 ), loss of appetite and fatigue ( 93. 9%, 62/66 ),sweating (47.0%, 31/66), myalgia and arthralgia (54. 5%, 34/66 ), hepatomegaly (27.3%, 18/66),splenomegaly (37.9%, 25/66), increased erythrocyte sedimentation rate (ESR) (62. 1%, 41/66), and elevated C-reactive protein (65.4%, 34/52). Average interval from onset to diagnosis was 19. 2 weeks. All 66 patients were positive in serum agglutination test for BruceUa, and 28 (42. 4% ) positive in blood or bone marrow culture for Brucella. Sixty-five of 66 patients were treated by standard combined treatment with rifampicin, quinolone, minocycline and streptomycin, with all effective, and only four with mild liver damage in treatment who got better after discontinuing medicines and changing treatment regimen.

Objective To develop the Occupational Embeddedness Scale for Nurses and test its re liability and validity.Methods The original scale was designed according to document analysis,openlabel questionnaire and interview results.After 3 sampling tests,the first predicted sample (n=368) was conducted with item analysis and exploratory factor analysis (EFA),the second predicted sample (n=306) was conducted with EFA,and then the formal tested sample (n=372) was conducted with confirmatory factor analysis (CFA).Another sample of 55 nurses was tested and retested two weeks later.The intestine emendatory Turnover Intention Scale was used to test its criterion validity.Results The final scale had 18 items and 3 dimensions,including fit,link and Sacrifice.Occupational Embeddedness Scale revealed good model fit indices (x2/df =2.563,RMR=0.048,TLI=0.914,CFI=0.925).The total scores was negatively correlated with the Turnover Intention Scale scores.The Cronbach's α coefficients of the whole scale was 0.917,and range 0.800～0.834 for each dimension.The test-retest reliability was 0.890,and ranged 0.732～0.873 for each dimension.Conclusions It suggests that the Occupational Embeddedness Scale for Nursing has good reliability and validity,and can be used to assess the occupational embeddedness status of nursing in China.

Objective To establish an effective qualitative discrimination method for Selaginella medicinal materials.Meth- ods Thin layer chromatography(TLC)method was used.Results The TLC method has a good specialization for identifying Selaginella medicinal materials and can distinguish Selaginella rnoellendorfii from other 10 familiar species in northern areas of Guangdong province.Conclusion The method can help to control the quality of Selaginella moellendorfii Tablet.