Objective To evaluate the therapeutic effects of autolngous bone marrow mesenchymal stem cells transplantation in the early phase of acute renal ischemia-reperfusion(I/R)injury in rabbits and their impact on tubular epithelial cell apoptosis.Method Bone marrow mesenchymal stem cells(MSCs)were isolated and cultured in vitro.Thirty rabbits were divided randomly into transplanted group,control group and sham-operated group,10 in each group.Rabbits of transplanted group were induced acute renal I/R injury by clamping bilateral renal arteries for 90 minutes,then transplanted MSCs labeled with BrdU immediately after the resumption of circulation in kidney.The rabbits of control group were induced acute renal I/R injury and then infused with saline instead.The sharn operated rabbits were transplanted autologous MSCs labeled with BrdU after sham operation.Fortyeight hours after transplantation,all rabbits were sacrificed.Renal functional and structural damage were evaluated.MSCs derived BrdU positive cells were determined by immunostalning.Tubular epithelial cell apoptosis was arialyzed by usign TUNEL.The SPSS version 11.3 Software was used for statistical analysis.ANOVA were used to ana lyze the data of renal function and tubular epithelial cell apoptesis.Data of renal structure darnage were analyzed by using the nonparametric Kruskal-Wallis test.Results Compared with control group,the renal functional and struc tural darnage were significantly ameliorated(24 h,BUN:F=7.483,Ser:F:15.091;48 h,BUN:F:17.741.Scr:F=61.865;P<0.05),tubular epithelial cell apoptods Wsa reduced significantly in transplanted group(F=135.495,P<0.01).MSCs derived BrdU positive cell were detected in the renal tissues of transplanted rabbits.Conelusiu Autologous bone marrow mesenchymal stern cells transplantation is curative for acute renal I/R injury in rabbits.Bone marrow MSCs might ameliorate renal I/R injury by reducing tubular epithelial cell apoptosis.

Objective To observe the expression levels of CD174, cyclin D1 and cyclin E in gastrointestinal tumor tissues, analyze the correlation among CD174, cyclin D1 and cyclin E, and evaluate the expression level of CD174 for diagnosis and prognosis of gastrointestinal tumors.Methods Flow cytometry was used to determine the expression of CD174, cyclin D1 and cyclin E on surface of the cells which were collected from the tumor tissues and distant part beside the same tumor and suspended. The expression and topography of CD174 in gastrointestinal tissue were investigated with immunohistochemical staining.Results The expression of CD174 in tumor tissues was obviously lower than that in distal noncancerous tissues (P

Aim To establish a new simple, easy and economical PCR method for determining cyp2d6~*10 allele and studying its distributive frequencies in Chinese population. Methods The new PCR are established and compared with the classical PCR-RFLP. The 224 samples have been determined with the new PCR.Results the results of the two methods go on the way. The allele distribution frequencies of cyp2d6~*10 resembles the reports. Conclusion the new method is proved to be accurate and convenient. It provides a genetics basis for the drug therapy with individuals in clinics.

Objective To explore the effects of fluvastatin on NF-?B activity and osteopontin(OPN) mRNA expression in albumin-induced renal tubular epithelial cells.Methods The renal tubular epithelial cells were cultured with 30 mg/ml fat free bovine serum albumin(BSA) as the control group.The renal tubular epithelial cells in the treatment group were cultured with different concentrations of fluvastatin for different hours.EMSA and RT-PCR were used to observe NF-?B activity and OPNmRNA expression.Results Fluvastatin can inhibit the NF-?B activity and OPNmRNA expression in a time and dose dependent manner.Conclusion Fluvastatin can inhibit NF-?B activity and OPNmRNA expression in albumin induced tubular epithelial cells.

AIM: To investigate the protective effect of rifampicin on rotenone-induced apoptosis of dopaminergic neurons and expression of ?-synuclein in rats.METHODS: Highly selective lesions and high expression of ?-synuclein in nigrostriatal dopaminergic neurons in rats were induced by chronic subcutaneous exposure to rotenone at dose of 1.5 mg?kg-1?d-1 for 3 weeks.At the same time,rifampicin was administered at dose of 30 mg?kg-1?d-1 by intragastric administration for 3 weeks.The changes of behavior,pathology and immunoreactivity of TH and ?-synuclein in SNc were observed.RESULTS: Obvious changes of behavior,pathology and TH immunoreactivity in SNc were observed in male SD rats injected subcutaneously with rotenone and rifampicin protected rats against these toxic effects induced by rotenone.CONCLUSION: Rifampicin has extensive protective effects against rotenone-induced neurotoxicity,which is related to inhibiting the expression and aggregation of ?-synuclein.

Abstract On August 11, 2022, Tongxiang Center for Disease Control detected a case of brucellosis, and the case was not engaged in related work. Case finding and risk factor investigation were immediately conducted to trace the source of infection. It was revealed that the case sold aquatic products at a farmer's market and frequently picked up goods at a seafood warehouse adjacent to a lamb slaughtering site. There was a potential risk of infection due to indirect contact with slaughtered lambs or contaminants. Serological tests were conducted on 9 employees of the slaughtering site and 48 residents nearby, and the brucellosis cases diagnosed in hospitals in the same area were searched. A total of 11 brucellosis cases were identified, including 9 confirmed cases and 2 asymptomatic infections. There were 2 cases of slaughtering workers and 9 cases of non-occupational individuals from the surrounding area of the slaughtering site. Brucella melitensis biovar 3 were isolated from a slaughtering worker and a non-occupational individual. The slaughtered lambs primarily came from northern regions such as Inner Mongolia and Heilongjiang. It was concluded that it was a cluster caused by Brucella melitensis biovar 3 and spread through direct or indirect contact with imported infected lambs or contaminated environments from a lamb slaughtering site. It is suggested to strengthen the quarantine of imported sheep, legally shut down non-compliant lamb slaughtering sites, implement designated slaughtering and enhance occupational protection.

AIM:To investigate the effects of osteogenic induction media and the medias containing different concentration of calcium on the induction of osteogenic differentiation of human renal fibroblasts in vitro.METHODS: Culturedhuman renal fibroblasts were divided into 5 groups in this experiment: osteogenic induction group (osteogenic inductionmedia), CaⅠgroup (0.5 mmol/L Ca2 + media), CaⅡgroup (1.5 mmol/L Ca2 + media), Ca Ⅲ group (2.5 mmol/LCa2 + media) and control group (PBS).The cell activity in each groups was measured by MTT assay .At 9th day, the cellcalcium Alizarin red S staining and alkaline phosphatase (ALP) Gomori calcium cobalt staining were performed respectivelyto observe the formation of calcium nidus and the expression of ALP .In addition, the expression of Runt-related transcriptionfactor 2 (Runx2) at mRNA and protein levels was determined by real -time PCR and Western blot, respectively.RE- SULTS: The remarkable positive signs which represented the formation of calcium nidus and the deposit of calcium objectsin all experiment groups were observed .The mRNA and protein expression of Runx2 in osteogenic induction group increasedin accordance with the induction time .Compared with control group, the mRNA and protein expression of Runx2 inthe CaⅠ ~Ⅲ groups increased gradually in a calcium concentration dependent manner at the 9th induction day.CON- CLUSION: Human renal interstitial fibroblasts show the potential activity in osteogenic differentiation induced by osteogen -ic induction media or high level calcium in vitro, which may be account for the cytological formation of the Randall ’splaque in the kidney.

emic euglycemic clamp quantitively.

Objective To investigate the expression of human glucocorticoid-induced tumor necrosis factor receptor(hGITR)in synovial and cartilage tissue of patients with rheumatoid arthritis(RA),and analyze their relationships with the severity of synovial inflammation in RA.Methods Expression of hGITR in synovial and cartilage biopsy material of 16 RA patients,9 osteoarthritis(OA)patients,4 contmIs were detected in situ by immunohistochemical technique and the results were analyze then.Results hGITR was strongly expressed in the vascular endothelial cells and inflammatory cells in RA and hGITR positive cells could be detected in approximately 69%of RA synovial cells.Significant differenee Was foand in hGITR expression between RA patients and control group(P<0.01).hGITR was less expressed in the cartilage than the synovial in RA.No significant difference was found in expression in the cartilage tissue expression between RA patients and the control group.Significant difference Was found in hGITR expression between synovial and cartilage in RA.Furthermore.the hGITR positive cells in synovial tissues were also found to correlate significandy with the severity of synovial inflammation in RA patients(r=0.895,P<0.01).Conclusion These results suggest that the abnormal expression of hGITR in the synovial my bean important mechanism leading to the synovial destruction found in RA.

Objective To establish the technique of hyperinsulinemic euglycemic clamp and to study the reference value of insulin sensitivity index in healthy Chinese. Methods According to the feedback mathematical model developed by DeFronzo, the technique of hyperinsulinemic euglycemic clamp was used in 90 healthy Chi- nese [ male:female =71 = 19; age; (28. 3±6. 1) years; body mass index (20. 9±1.5) kg/m2 ] to study die glu-cose metabolized rate. Blood samples were obtained at timed intervals in the fasting state and during the clamp for the measurement of glucose, insulin and C peptide. Results During the clamp tests, the blood glucose levels were con-trolled within 10% of target value. The coefficient of variation of glucose levels was 3. 8% 0.1%. In the steady state, the insulin sensitivity index (glucose metabolized rate, M value ) was (7.78±2.30) mg· kg-1 min-1, which was distributed normally. The lowest quartile of M value was 6. 286 mg·kg -1 min-1'. The coefficient of variation of M value was 9.4%±2.8%. Conclusion The technique of hyperinsulinemic euglycemic clamp and the reference value of insulin sensitivity index in healthy Chinese are successfully established in our center.