Aim To investigate antiaging effects of beta-carotene(?-C) from dunaliella salina on fruit flies and rats and to probe possible mechanisms.Methods The whole study contains fruit flies experiment and rats experiment.In the fruit flies experiment,fruit flies were divided into nine groups according to different ingredient in the nutrient medium.We took life span,flight capability,the number of copulatory fruit flies aged 8 days,and head MDA content of fruit flies aged 40 days as indexes of aging.In rats experiment, 54 aged male rats were divided randomly into 6 groups and bred for 45 days with different diet.Plasma MDA,CAT and GSH-Px were detected.MDA,SOD and GSH-Px in heart,liver,pulmo,lien and brain were also detected.Results Compared with control group in fruit flies experiment,the shortest life span,maximum life span and mean life span were increased in each experimental group.Flight rate and flight frequency were increased,the number of copulatory fruit flies was also increased.The content of MDA was significantly decreased.In rats experiment,plasma MDA content was significantly decreased.The activity of GSH-Px was elevated.Similar results were obtained in different tissues.MDA content was significantly decreased in each tissue sample,while activities of SOD and GSH-Px were significantly inceased.Conclusion ?-C from dunaliella salina had antiaging effects on fruit flies and aged rats.The possible mechanism resided in its antioxidative effects owing to the elevated antioxidases.

Objective: To investigate effect of Zhuanggu Granula medicated serum on proliferation and differentiation of bone marrow stromal cells(BMSCs) in vitro. Methods: Isolated BMSCs were cultured in vitro. The cells were treated with Zhuanggu Granula medicated serum, and the blank serum as the control group. The proliferation of cells was detected by MTT assay, the macroscopic histology, HE staining and immunohistoc-hemical examinations. Results: Zhuanggu Granula medicated serum effectively stimulated BMSCs proliferation (P

Based on theoretic analysis, a new method for measuring linearity of physiological instrument and its inner unit circuits is provided. In this method, the sawtooth wave signal, which includes a base wave and its odd-even times harmonic waves, is used to act as input signal, then the linearity of physiological instrument and its inner unit circuits can be measured by analyzing the shape and frequency property of the output signal. The results of experiment show this method is quick and convenient for measuring the linearity of physiological instrument and its inner unit circuits.
Diagnosis, Computer-Assisted ; methods ; Humans ; Monitoring, Physiologic ; instrumentation ; Signal Processing, Computer-Assisted