Objective To explore the effects of post-discharge continuous nursing intervention on medication compliance and negative emotion of patients with acute myocardial infarction after percutaneous coronary intervention (PCI).Methods 168 patients with acute myocardial infarction were selected.All of them got emergency PCI after admitted.Then,they were simply randomized into observation group (84 cases) and control group (84 cases) using coin toss method combined with random comparison table.The observation group accepted continuous nursing intervention after discharged,including regular home visits,telephone feedback and clinical visits.The control group got a regular clinical follow-up.Their medication compliance and negative emotion were investigated after 1 month,3 months,6 months and 12 months from discharged,respectively.Results Relevant data with different periods of patients with repeated measurement data analysis of variance,discharge for 3 months,6 months and 1 year,compared with the control group,the medication compliance of the observation group increased obviously,the SAS and SDS scores decreased obviously,the differences were statistically significant (t =1.432,2.781,1.432,2.781,2.504,3.783,2.761,4.621,4.884,all P ＜ 0.05).Further analysis of the data between inter group effect,time effect,inter action effect showed that with the extension of the discharge time,the medication compliance and SAS,SDS scores of the two groups were significantly different (F =30.258,P ＜ 0.01;F =2.673,P ＜ 0.05;F =2.024,P ＜ 0.05).In hospital,there were no statistically significant differences in the quality of life of patients between the two groups(t =1.250,1.250,1.250,1.250,0.694,1.113,2.463,all P ＞ 0.05).1 year after discharge,compared with the control group,the movement and exercise,interpersonal relationships,nutrition,health responsibility,self-actualization,stress management scores in the observation group significantly increased,the differences were statistically significant (t =2.463,3.146,2.463,3.146,2.633,2.115,all P ＜ 0.05).Conclusion Continuous nursing interventions can effectively reduce acute myocardial infarction patients'anxiety and depression after PCI,improve their compliance and prevent recurrent cardiovascular events.

OBJECTIVE:To study the relative bioavailability of yifuxian'an capsules.METHODS:Concentrations of ri-fampicin,isoniazid and pyrazinamide in plasma were determined on12volunteers respectively after a single oral dose of yifux-ian'an capsules(tested preparations)and rifater(reference preparation)by RP-HPLC method;the pharmacokinetics parame-ters were processed by3p97process.RESULTS:The AUC 0～t of the tested preparation and reference substance of rifampicin were(70.69?36.32)and(70.77?32.26)(?g?h)/ml respectively,t max of which were(1.82?0.28)and(1.79?0.25)h re-spectively,C max of which were(12.83?4.98)?g/ml and(12.84?3.95)?g/ml respectively;The AUC 0～t of which were(14.30?8.25)and(13.76?6.16)(?g?h)/ml respectively,t max of which were(1.54?0.36)and(1.58?0.38)h respectively,C max of which were(5.42?2.52)and(5.46?2.46)?g/ml respectively;The AUC 0～t of which of pyrazinamide were(243.56?128.98)and(238.07?117.63)(?g?h)/ml respectively,t max of which were(1.58?0.64)and(1.69?0.55)h respectively,C max of which were(29.45?12.45)and(27.46?11.02)?g/ml respectively.The relative bioequivalence of rifampicin,isoniazid and pyrazi-namide of the tested preparation were(99.88?12.92)%,(103.84?24.10)%and(102.57?12.31)%respectively.CONCLU-SION:Bioavailability of the2preparations are bioequivalent.

X-ray cephalometry was used to analyze craniofacial morphology in 32 males and 23 females with operated cleft palate. They underwent primary palatoplasty at the ages of 4 to 7 years and examination at the age of 18 to 25 years old. The cephalometric data were compared with that of the native Hubei adults with normal occlusions. The results indicated the cranial base in the cleft group was morphologically similar to that of the normal controls, there were a decreased angle of skeletal facial convexity, a shorter maxilla, an increased gonial angle and a more open mandibular plane angle in the clefts. All of these differences were significant. Surgical treatments at the age of 4 to 7 years old could cause craniofacial deformities at adults.

Objective:To investigate the therapeutic effect of intravascular balloon emolization of carotid-cavernous fistula.Materials and Methods:7 cases(5 of traumatic origin, and 2 spontaneous),undewent DSA of brain vessels showing the exact sites of fistulae.The key points of angiography in this procedure were described.Results:5 of the 7 cases complete patency of ICAs.I case resulted in occlusion of ICA,and another of type D was unsatisfactory. Conclusion:DSA of brain vessele could dipiot the site,size and type fistula.Most cases of simple carotid-cavernous fistula are of traumatic arigin and intravascular balloon embolization should be the first-choice of treatment

Objective To evaluate the feasibility, safety and efficacy of a new homemade device for percutaneous closure of secundum atrial septal defects (ASDs) Methods Percutaneous closure of surgically created fossa ovalis ASD was attempted in 8 piglets The device was braided from Nitinol wires in the shape of two flat discs with a short connecting waist with a diameter corresponding to that of the defect to be closed Polyethylene filling was implanted to enhance thrombogenicity Pulmonary arteriography with levo phase was obtained before closure and immediately after closure Transthoracic echocardiography with color Doppler was used in all animals during closure and in follow up examinations The animals were killed at various intervals up to 6 months after occlusion for microscopic examination Results Successful placement of the device was achieved in all the 8 animals All ASDs were completely occluded in 1 month, observed by transthoracic echocardiography Postmortem gross and microscopic examination of the 8 specimens 1 to 6 months after placement showed complete or near complete fibrous neoendocardium coverage of both the right and left atrial discs Conclusion These observations show the feasibility of occlusion of secundum ASDs by the homemade device with the advantages of easy placement and repositionability in piglets

0.05). Conclusion The polymorphism of G-944C in CⅡTA gene promoter Ⅳ was associated with the susceptivity of chronic HBV infection, but was not associated with severity of diseases. The individuals with chronic HBV infection of CC genotype are of less possibility to develop chronic liver disease than those of other genotypes.

Objective To investigate the distribution of rick factors between intracerebral hemorrhage(ICH) and coronary heart disease (CHD) in Tongliao city in Innermogolia. Methods Medical records(departments of medical neurology and cardiovascular internal medicine in 2003~2005) were randomly selected from 6 general hospitals above the second class in Tongliao city Innermogolia .All the risk factors of the cartain diseases were carryed on retrospective investigation analysis. Results All the survey index have significantly statistical difference in the basic data. Single-factor non-conditional Logistic regression analysis showed 10 risk factors including the gender, age, nation, smoking, hypertension, history of hypertension, alcohol drinking, glucose(GLU), history of diabetes and triglyceride(TG) have significantly statistical difference. These 10 risk factors were taken into multifactor stepwise regression model. 8 risk factors( gender, age, smoking, history of hypertension, history of diabetes,GLU,TG and hypertension) had significantly statistical difference. Conclusions Compared with CHD, the influence of age, smoking, history of diabetes and TG are lower, and the influence of the gender, the history of hypertension, GLU and hypertension are higher in ICH.

Objective·To investigate the effects of insulin-like growth factor 1 on prion encoding gene (PRNP) expression and amyloid precursor protein (APP) metabolism of PC12 cells. Methods·After PC12 cells were treated with 20, 40, 80 ng/mL IGF-1 for 24 h, real-time PCR was used to detect the mRNA expression levels of PRNP, and Western blotting was used to detect the protein levels of AKT, phosphorylation of AKT (pAKT), ERK and phosphorylation of AKT (pERK). The level of β-amyloid 42 (Aβ42) in supernatant fluid was detected by ELISA. Results·Compared with the blank control group, the expression of PRNP mRNA in Alzheimer's disease (AD) model group was increased significantly (P<0.01). The expression of PRNP mRNA was significantly increased after cells were treated with 40 and 80 ng/mL IGF-1 for 24 h (P<0.01). There was no significant difference in APP protein expression among AD model group and three IGF-1 treatment groups (P>0.05). Compared with the blank control group, the level of Aβ42 in supernatant fluid of model group was decreased significantly with the increasing of IGF-1 concentration. The expression level of Aβ42 was decreased significantly in 40 and 80 ng/ml IGF-1 treatment group (P<0.05). The expression of AKT/pAKT, ERK/pERK in AD model group was significantly increased along with the increasing of IGF-1 concentration (P<0.05). Conclusion·IGF-1 could regulate the expression of PRNP gene and effect the metabolism of APP, which may be associated with PI3K/AKT, MAPK/ERK1/2 signaling pathway.

OBJECTIVE:To explore the medication rules of TCM complex formulas for anti-experimental liver injury. METH-ODS:Usingexperimental liver injuryTCM complex preparationsprotection effectas themes,related literatures about medi-cation rules of TCM complex formulas for anti-experimental liver injury in CJFT,Wanfang database and VIP database were re-trieved,and general information,distribution,use frequency and co-occurrence frequency of single herbal medicine were descrip-tively analyzed. K-means clustering model and Apriori in SPSS Modeler were adopted for cluster analysis(clustering side and im-portance)and correlation analysis in medicines with top 10 use frequency. RESULTS:498 related literatures were retrieved,and 70 valid literatures were involved totally,including 51 TCM complex formulas and 111 Chinese herbal medicines. It was mainly drug for clearing heat,restoring vital energy and blood circulation. Drugs for clearing heat were most widely used(34 varieties),drugs for restoring vital energy showed the highest use frequency,with 63 times(25.82%);Prunella vulgaris had the highest frequency in single herbal medicines(accounting for 25.49%);and P. vulgaris-Salvia miltiorrhiza had the highest co-occurrence frequency in drug pairs. 5 clustering sides were obtained in clustering analysis,which were P. vulgaris-S. miltiorrhiza-Angelica sinensis- Glycyr-rhiza uralensis,Astragalus membranaceus-A. sinensis-Hedyotis diffusa,Artemisia scoparia-Curcuma wenyujin-Plygonum cuspida-tum,P. vulgaris-S. miltiorrhiza-A. scoparia,Schisandra chinensis-G. uralensis. Importance prediction showed,S. chinensis was the most important and P. cuspidatum was the least important. 13 drug pair rules were obtained from correlation analysis,and both sup-port and confidence degrees were low. CONCLUSIONS:The TCM complex formulas for anti-experimental liver injury are mainly based on drugs for clearing heat and restoring vital energy,while the compatibility is varied,and correlation degree is relatively low.

Aim To investigate the effect of dipfluzine on hERG potassium currents expressed heterologously in xenopus oocytes.Methods Using Xenopus oocytes expression system,the current amplitude and kinetic characteristics of hERG were measured with the two electrode voltage-clamp technique before and after dipfluzine application.Results Dipfluzine(8 nmol?L-1~5 ?mol?L-1)concentration-dependently inhibited hERG currents;the concentration for half maximal inhibition(IC50)was 98.0 nmol?L-1.Dipfluzine-induced inhibition of hERG currents was voltage dependent at membrane potentials between-10 and 40 mV.Dipfluzine at 1 ?mol?L-1 didn't statistically shifted V1/2 of hERG currents activation.Dipfluzine at 1 ?mol?L-1 significantly decreased the activating time constants and the deactivating time constants,and enhanced hERG currents activation and deactivation.Conclusion Dipfluzine concentration-dependently inhibits hERG currents and modifies kinetic characteristics of hERG activation and deactivation,which may be correlated with its antiarrhythmic effect.