Objective To investigate the effect of vitamin K3 decreasing the urine oxalate excretion in rats. Methods A total of 80 male Sprague-Dawley rats (body weight, 200-250g) were randomly divided into 8 groups, ie, control group, only vitamin K3 group, stone forming group, stone forming plus 4.0、3.0、2.0、0.8、0.4mg/d Vit K3 group. Each group is 10 rats respectively. The change of urine oxalate was observed. Results Vitamin K3 can reduce the 24h urine oxalate excretion in stone-forming group rats, but there were no effects in control group rats(P

Objective To investigate the effect of different doses of Vitamin K3 on the expression of osteopontin in rats kidney. Methods After different dose of Vitamin K3 were injected to different groups of rats feeded with same stone-inducing agent, the level of expression of OPN in renal tissues was observed with immunohistological staining. Results OPN expression located at distal convoluted tubule when the rats feeded without stone-inducing agent, and the OPN expression stain was extended to proximal convoluted tubule when the stone-inducing agent was feeded. Vitamin K3 can decrease the extension of OPN expression induced by the agent and more doses of Vitamin K3, more effects. Conclusions Vitamin K3 can decrease the expression of OPN in the kidney tubule of rats feeded with stone-induced agent.

0.05 . In combined test, the combined OR= 3.164 , OR = 3.209 and its 95% confidence interval was in 1.390 ～ 7.200 and 1.747 ～ 5.900 , respectively. CONCLUSION: The case fatality rates in combination group were significantly lower than those in single somatostain group. The efficacy of combination group was superior to that of single somatostain group.

Objective To investigate effects of pedicle processing methods on the survival and appearance of the pedicle flaps. Methods The study involved 17 patients with soft tissue defects of extremities treated with the “exclamation mark” shaped pedicle flaps from October 2009 to January 2011.The traditional pedicled rotation flaps was designed to be a tennis racket-like shape,then the rotational pedicle was designed to small round tension reduction flap and pedicled rotation flaps,which contributed to formation of the “exclamation mark” shaped pedicle flaps.The soft tissue defects was located at the upper extremity in three patients and at lower extremity in 14,with defect areas of 4.0 cm× 7.2 cm-5.8 cm × 14.0 cm.There used two superficial radial nerve vasotrophic flaps,one ulnar artery supra-carpal cutaneous flap,eight sural neurocutaneous vasotrophic flaps and six saphenous nerve vasotrophic flaps.Results All flaps survived at Ⅰ stage except that one flap had partial distal necrosis postoperatively,which was cured after dress change.The follow-up ranged from six months to one year,which showed unobvious swelling of the survived flaps and good appearance of the rotation point of the pedicle. Conclusion The “ex(c)lamation mark” shaped pedicle flaps can reduce entrapment of the distal pedicle,promote venous drainage,improve survival and modify the appearance of the flaps.

Objective To investigate the X-ray and clinic manifestations of hereditary multiple osteochondroma(HMO).Methods Five patients with HMO clinically and pathologically confirmed and 63 persons in their pedigree were studied.Results Of 63 cases inquired in this study,there were 21 patients with hereditary multiple exostosis,including 16 men and 5 women.In 5 cases confirmed pathologically,4 patients had hereditary history of pedigree and the members of three generation in 1 patient with HMO had no the history of pedigree.Conclusion HMO has the remarkable sex difference and the typical inherited trend including the location of exostosis.HMO has inherited character of skipping generation.

Objective To evaluate the clinical effect of lumbar intervertebral disc herniation (LIDH) by CT-guided precise injection around nerve root and in epidural cavity. Methods One hundred and eight patients of LIDH were treated by CT-guided precise injection around nerve root and in epidural cavity, and followed-up after 1, 3 and 6 months. Visual analogue score (VAS) for pain was used to evaluate the efficacy before and after treatment. Results In 108 cases, there were 83 patients (76.9%) with good result whose improvement of VAS was greater than or equal 50% after treatment 1, 3 and 6 months. There were 19 patients (17.6%) with fair result whose improvement of VAS was less than 50% after treatment 1, 3 and 6 months. There were 6 patients (5.6%) with invalid result whose improvement of VAS was unobvious after treatment 1, 3 and 6 months. Conclusion CT-guided therapy of LIDH by precise injection around nerve root and in epidural cavity is safe, accurate, effective, minimally aggressive technique and worth being practiced clinically.

Aim To observe the effects of total flavones of hippophae rhamnoides L on myocardial ischemia-reperfusion injury and the MMP-9 and TIMP-1 expression in cardiac tissues of rats,and to explore the protective mechanism of total flavones of hippophae rhamnoides L on myocardial ischemia-reperfusion injury.Methods Fifty rats were classified into 4 groups by using the random grouping principle:model group,losartan group,sindacon group of different doses and sham operation group.The myocardial ischemia-reperfusion injury model was established by ligating left anterior descending coronary artery for 30 minutes and releasing then.The cardiac muscle tissue was stained by HE to observe its necrosis area and pathological changes as well as the expression of MMP-9 and TIMP-1by immunohistochemistry method.Results HE sections showed that necrosis of cardiac muscle in rats was significantly relieved in sindacon group by using different doses compared with model group,and immunohistochemistry sections showed that the sindacon group using different doses decreased the expression of MMP-9 compared with the model group and increased the expression of TIMP-1.Conclusion Sindacon has protective effect on cardiac muscle after the myocardial ischemia-reperfusion injury,and its mechanism may be related to its inhibition of the MMP-9 and increasing TMPM-1 expression in cardiac muscle.

ObjectiveTo study the using of cerrobase as the compensation material in the intensitymodulated radiation therapy (IMRT) implementation and impact factors.MethodsWith therapy planning system (TPS) exported the radiation field intensity file (Dicom RT),through measuring the attenuation coefficient of cerrobase,to calculate the processing depth of AUTIMO 3D CNC corresponding for Dicom RT files at each pixel,then using the processed foam casting of Cerrobase,produced the required IMRT compensator.Through the MATRIXX testing the IMRT compensator in clinical implementation.At the same time we compared the MU of using multi-leaf collimator (MLC) and Cerrobase IMRT compensator for 10patients.ResultsWith cerrobase compensation IMRT can get similar dose or dose distribution to dose produced by TPS for point or plane dose,error is within 5％.To comparison with MLC,using cerrobase compensator has fewer treatment times ( (4.44±0.39) min:(5.71±0.57) min (t =10.82,P =0.000) )and fewer MU (462.5 ± 65.8) MU:(524.5±99.6) MU(t=3.14,P=0.012) ).Conclusions Comparison with MLC IMRT,the cerrobase compensation technique has an important application value with its unique advantages.This research provides an implemented method of IMRT radiotherapy for the primaryhospital.

Objective To evaluate the effect of perioperative administration of dexmedetomidine on postoperative ileus after laparocolectomy.Methods Sixty patients scheduled for abdominal surgery were randomly divided into two groups,30 in each group.Group D received dexme-detomidine administeration at a loading dose of 0.6 μg/kg for 10 minutes before induction,followed by an infusion rate of 0.5 μg·kg-1 ·h-1 to 30 min before the end of surgery.The control group re-ceived saline instead of Dex.After the surgery,Group C received intravenous sufentanyl 2 μg/kg, while group D sufentanyl 2 μg/kg combined with Dex 2 μg/kg.Heart rate variability (HRV)were detected before Dex infusion (T0 ),10 minutes after intubation (T1 ), 10 minutes after CO 2 insufflation (T2 ),1 hour after CO 2 insufflation (T3 ),10 minutes after CO 2 desufflation (T4 ),and 10 minutes after extubation(T5 ).The plasma concentrations of epinephrine(E)and norepinephrine (NE)were determined at T0 ,T3 ,T5 ,T7 and T1 0.The recovery of bowel function was evaluated in terms of the first time to fart and intake food.Results Compared with T0 ,HRV of power (TP), high-frequency (HF)power,low-frequency (LF)power and the ratio of LF/HF power were signifi-cantly decreased at T1-T4 in group C and at T1-T5 in group D.The plasma concentrations of E and NE were higher at T3 ,T5 ,T7 and T1 0 in both group C and group D (P <0.05).Compared with T1 ,TP, LF and the ratio of LF/HF were increased at T2-T4 (P <0.05).Compared with group C,TP,LF and the ratio of LF/HF were decreased at T2-T5 ,The plasma concentrations of E and NE were decreased at T3 ,T5 ,T7 and T10 and the time of first flatus was earlier(P <0.05).Conclusion The perioperative ad-ministration of dexmedetomidine during laparocolectomy facilitated the early recovery of bowel func-tion after surgery and decreasede the time of postoperative ileus.

Objective To explore the elementary properties of LiF (Mg,Cu,and P) thermoluminescent dosimeter (TLD) in dosimetry for tumor radiotherapy,and to provide a reliable basis for thermoluminescent dosimetry in clinical radiotherapy.Methods 60Coγ-ray and 6 MV X-ray were used for evaluation of the dispersion,repeatability,and dose response of LiF TLD.To meet the requirement of clinical radiotherapy,the effects of energy,dose (50-600 cGy),radiation dose rate (50-600 MU/min),and the angle of incidence for accelerator gantry (0°-± 90°) on TLD were determined and the respective correction factors were calculated.Results The errors of repeatability and dispersion of TLD were less than ±3％.There was a linear relationship between readout and irradiation dose within a certain range of irradiation dose.The minimal relative deviation of 0.3％ was obtained when 6 MV X-ray was used with a dose rate of 300 MU/min and an angle of incidence of 0°.Conclusions LiF TLD shows excellent repeatability,low dispersion,high stability,and strong linear correlation.It meets the international criteria and can be used for dose measurement in tumor radiotherapy.