Objective: In this study,we evaluatedthe influence of different nutritional support routes and nutrients on the intestinal barrier function and bacterial translocation in rats with gut I/Rinjury. Methods: Eighty rats were divided randomly into: I/R group,control group,and nutrition-supportgroups. Bacterial culture, D-lactate and endotoxin levels was assayedafter 7 days of nutrition support. Results: There was serious damagein intestinal tissue of I/R group. Remarkably higher value of bacterial translocation occurred in PN group (P0.05).The endotoxin level was the highest in PN group. Conclusions: TPN alone could impair gut immune barrier function and thereforefacilitate bacterial translocation.The use of immunonutritionindicates limited efficacyin preventing bacterial translocation compared with common enteral nutrition.