OBJECTIVE:To study the inhibitory effects of emodin on the proliferation of human hepatocellular carcinoma SMMC7721 cells. METHODS:SMMC7721 cells were treated with 0(negative control),25,37.5,50,62.5,75,87.5,100μmol/L emodin solution and 100 μmol/L 5-FU for 24 h,48 h,72 h. The optical density value of cells was detected,and inhibition rate was calculated. SMMC7721 cells were treated with 0 (negative control),25,50,75 μmol/L emodin solution and 100 μmol/L 5-FU for 48 h,and cell apoptosis rate,cell cycle and the expression of Bax and Bcl-2 gene were detected. RESULTS:Compared with negative control,the rate of cell proliferation inhibition increased after treated with 25,37.5,50,62.5,75,87.5,100 μmol/L emodin and 100 μmol/L 5-FU,which was positively associated with the concentration and duration. Compared with negative con-trol,the rate of cell apoptosis increased after treated with 25,50,75 μmol/L emodin solution and 100 μmol/L 5-FU;the expres-sion of Bax increased and that of Bcl-2 dereased;50,75 μmol/L emodin solution and 100 μmol/L 5-FU could arrested cells at G0/G1 phase(P<0.05 or P<0.01). CONCLUSIONS:Emodin can inhibit the proliferation of SMMC7721,promote cell apoptosis and in-hibit cell growth.

Bidirectional signal transduction is a newly elucidated mechanism in intercellular communication. The bidirectional signal transduction mediated by the Eph-ephrin is an important representative in this field. The Eph family receptor tyrosine kinases and their membrane-bound ligands, the ephrins, play pivotal roles in the development of nervous system, angiogenesis, etc. The signal transduction into cells by Eph receptors is the forward signal, whereas the signal transduction by ephrins is the reverse signal. Based on their molecular structures, the ephrins can be divided into two subclasses, i.e. ephrinA and ephrinB. The ephrinBs are transmembrane proteins, which can activate FAK, JNK and Wnt signal transduction pathways through phosphotyrosine-dependent signaling and PDZ-binding motif-dependent signaling. The ephrinAs are glycosylphosphotidylinositol (GPI) anchored proteins, which can also mediate reverse signal transduction.

The incidence of early neurological deterioration in acute ischemic stroke is higher, and the clinical prognosis is poor. There is no effective specific prevention and treatment now. This article reviews the advances in research on early neurological deterioration in acute ischaemic stroke in recent years.

Objective Patient safety is key to quality of care in hospital management, for which drug safety is critical. General hospitals are challenged with how to monitor drug adverse reaction (ADR), and motivate their staff to report ADRs in building a hospital safety culture. Methods The PDCA cycle quality improvement process was called into play, to set the ADR criteria and analyze data of inpatients' ADRs from 2005 to 2009, which was followed by measures proposed for ADR continuous improvement. Results A total of 2850 ADR cases were reported in the past 5 years, averaging 570 cases per year. The ADR incidence rate was 3. 28%, of which 43 were severe ADR cases, or 0. 49% of the total cases, accounting for 1. 51% of all the ADR cases. Year to year, the quantity and quality of ADR reporting have been improving in the hospital, witnessing an initial culture of drug safety. In 2008, the hospital was awarded as an advanced ADR center in Beijing. Conclusion The PDCA cycle is an effective means for ADR management, as it can improve the awareness of the medical staff for proactive ADR reporting. Sustained circular management against ADRs can minimize risks and maximize patient safety.Under-reporting and the corrective measures shall be watched over by the hospital in the future.

Objective To investigate the values of diffusion tensor MR imaging(DTI)and fiber tractography(FT)in the assessment of cognitive functions in children with periventricular leukomalacia (PVL),to facilitate to understand the mechanism of PVL children's cognitive functions.Methods Forty six children with PVL and 16 age-matched volunters were recruited.Developmental quotient(DQ)of all cases was recorded.According to the DQ scores,patients were divided into 3 groups:mild in 11 patients,moderate in 19,and severe in 16.DTI was performed in all cases and fractional anisotropy(FA)values were be calculated.Non-paired t test was used to compare the FA values of major white matter fibers between patients group and controls group.One-way ANOVA was used to compare the FA values among 4 groups.Results Compared with controls,all 46 patients with PVL showed a significant mean FA reduction in left corticospinal tract(CST)(0.476 ±0.064 vs.0.531 ±0.064),bilateral anterior limb of internal capsula (ICAL)(left 0.357 ±0.050 vs.0.405 ±0.081,right 0.370 ±0.040 vs.0.405 ±0.036),posterior limb of internal capsule(ICPL)(left 0.483 ±0.044 vs.0.546 ±0.091,right 0.485 ±0.046 vs.0.547 ±0.083),arcuate fasciculus(AF)(left 0.367 ± 0.069 vs.0.433 ± 0.097,right 0.363 ± 0.064 vs.0.421 ±0.091),posterior thalamic radiation(PTR)(left 0.390 ± 0.059 vs.0.459 ± 0.067,right 0.382 ± 0.047 vs.0.446 ± 0.064),anterior CG(ACG)(left 0.362 ± 0.056 vs.0.423 ± 0.057,right 0.345 ± 0.056 vs.0.417 ± 0.080),superior longitudinal fasciculus(SLF)(left 0.323 ± 0.050 vs.0.426 ±0.102,right 0.341 ± 0.056 vs.0.416 ± 0.092)and splenium of the corpus callosum(SCC)(0.535 ±0.090 vs.0.606 ±0.060)(t =2.037-3.745,P ＜0.05).The severity of cognitive impairment companied with decrease of the FA values of all fibers.The FA values of bilateral CST,ICAL,ICPL,left AF,bilateral PTR,ACG,SLF and SCC were significantly different among different groups(F =3.021-7.146,P ＜ 0.05).Conclusions The damage widespread of white matter bundles is found in children with PVL DTI and FT can provide details in the assessment of cognitive impairment in children with PVL.

During spermatogenesis, lysine-rich histones in spermatogenic cells are progressively replaced by arginine-rich protamine. In this study, the nucleoprotein transition in spermatogenic cells of Wistar rat was investigated using aniline blue staining, PTA staininig (both to demonstrate lysine-proteins) and NQS (1, 2-naphthoquinone4-sodium sulfonate) staining (to demonstrate arginine-protein). Under light microscope, the nuclei of spermatogonia and spermatocytes were intensely aniline blue positive and the nuclei of young spermatids moderately positive, while the nuclei of late elongated spermatids and spermatozoa were aniline blue negative. The nuclei of spermatogonia and young spermatids were basicallyy NQS negative and there were a few granules of weak NQS positive in the nuclei of primary spermatocytes, while the nuclei of late elongated spermatids and spermatozoa were NQS positive. Under the electron microscope, the PTA positive chromatin in the nuclei of early spermatids with round nucleus (steps 1 to 8)was fibrillar or granular in appearance. Along with the condensation of nuclei of spermatids(steps 9 to 13), the nuclear stain ability increased. The positive chromatin in the nuclei of spermatids (steps 14, 15) was disappeared progressively, in the direction from cranial to caudal along with the further condensation of nuclei. The nuclei of late spermatids with elongated nucleus (steps 18, 19) and spermatozoa were PTA negative. These observations suggest that the nucleoprotein transition from histones to protamine (S_1) occur during spermiogenesis and that this process could be divided into two consecutive steps, i. e. from histones through an intermediate phase of transition proteins to protamine.

BACKGROUND: Flash visual evoked potential(fVEP) can reflect the integrity of visual pathway from retina to pulvinar cortex. The utilization of its characters can accurately evaluate the injury situation of visual conduction pathway.OBJECTIVE: To investigate the feasibility and effects of fVEP on non-traumatic monitoring of intracranial pressure.DESIGN: A repeated measurement design based on patients.PARTICIPANTS: Totally 197 patients with brain injury including 138males and 59 females with an average of(38 ±9) years old were selected from the First Affiliated Hospital of Chongqing Medical University and Xuanwu Hospital Affiliated to Capital University of Medical Sciences. The selected diseases were: epidural hematoma( n=20),subdural hematoma(n = 26),cerebral contusion( n = 4),subdural hematoma companied with cerebral contusion( n = 92),and cerebral contusion companied with intracerebral hematoma( n = 55).METHODS: The level of intracranial pressure of 197 patients with brain trauma was detected with fVEP and epidural manometry. Data of traumatic surveillance were collected after the completion of fVEP. Changes of blood pressure and heart rate in patients were recorded simultaneously.MAIN OUTCOME MEASURES: fVEP results,and levels of intracranial pressure.RESULTS: The average intracranial pressure of 197 patients with brain trauma was(2.75 ± 0.64) kPa,( 1.54 - 4. 02 kPa) . The dispersion of both traumatic and non-traumatic surveillance was relatively big in patients with confirmed visual pathway injury. No typical wave was induced by fVEP in2.79% of patients. Scatter diagram suggested that the consistence between fVEP and epidural manometry was relatively good. Linear regression analysis showed that heart rate was the most sensitive one to the fluctuation of intracranial pressure (β = -0. 369) followed by systolic pressure (β= 0. 316),while diastolic pressure was not so sensitive (β = 0. 147). There was negative correlation between heart rate and intracranial pressure,which could quite sensitively reflect the fluctuation of intracranial pressure,systolic pressure was the next,and diastolic pressure was not easily to be affected by the changes of intracranial pressure.CONCLUSION: Non-traumatic surveillance,fVEP,has favorable consistence with traumatic detection,which can quantitatively evaluate the changes of intracranial pressure non-traumatically.

Objective To analyze the separation and displacement of bone fragments in sagittal fracture of the mandibular condyle (SFMC),understand the motion type and guide clinical treatment and development of related surgical instruments.Methods Forty-five patients (52 sides) with SFMC treated between October 2008 and October 2012 were enrolled; 32 patients (36 sides) were males and 13 patients (16 sides) were females.According to the Neff classification,there were 20 sides with type A,28 sides type B and 4 sides type M.All the patients underwent CT scan and were classified based on measurements of distance and angle of displaced bone fragments.Results Patients were categorized into type Ⅰ on 20 sides (type Ⅰ A on 14 sides and type Ⅰ B on 6 sides),type Ⅱ on 27 sides (type Ⅱ A on 11 sides and type ⅡB on 16 sides),type ⅢB in 1 side and type Ⅳ in4 sides.Mean displacement of bone fragments was 12 mm (range,4-16 mm) ; mean deviation angle was 34°(range,11°-43°) ; mean descending distance was 13 mm (range,6-18 mm).Conclusion CT analysis results show that,mast bone fragments in SFMC move slightly.

Aim To explore the antioxidation of pergolide on rat models of Parkinson disease and its mechanism. Methods Wistar rats were pretreated daily with pergolide(1.0 mg?kg -1,ip),pergolide combined with sulpiride(25 mg?kg -1) or sulpiride alone for 7 days, and then sacrificed by decapitation. The activity of SOD and GSH in striatum was determined. Unilateral Parkinsonian models were made in rest of the rats by stereotaxically injecting 6-OHDA. After two weeks, apomorphine (APO)-induced rotational behavior of each group was compared, and the concentrations of striatal dopamine (DA),dihydroxyphenyl acetic acid(DOPAC) and homovanilic acid(HVA) were determined by high performance liquid chromatography (HPLC). Result Administration of pergolide for 7 days significantly increased the act- ivities of SOD and GSH in the striatum (P

AIM:To explore the effects of the new drug of sulfonylurea(1-{4-[2-(3-ethyl-4-methyl-2-oxo-3-pyrroline-1-carboxamido)ethyl]-phenylsulfonyl}-3-(1,4-tetramethylene)-urea,BGW) on the glucose uptake and the activation of Akt/PKB in SMMC7721 cells.METHODS:Cultured SMMC7721 cells were divided into control group,glibenclamide group,insulin group,BGW group and BGW+insulin group.Scintillation was used to detect the glucose uptake in SMM7721 cells.The activation of Akt/PKB was tested by Western blotting.RESULTS:Compared to control cells,gibenclamide,insulin,BGW and BGW+insulin significantly increased the glucose uptake(P