Objective To investigate the association between serum uric acid (SUA ) level and diabetic peripheral neuropathy (DPN ) in middle‐aged and elderly population. Methods A total of 207 middle‐aged and elderly patients with T2DM were enrolled in this study. All the subjects were divided into two groups:T2DM only group and DPN group. The DPN group was further divided into three subgroups (mild ,moderate and severe group) according to TCSS score. Pearson correlation analysis was used to evaluate each index ,and binary logistic regression was used for statistical analysis of risk factors for DPN.Results SBP ,UACR ,BUN ,Scr ,TC ,LDL‐C and SUA were significantly higher in DPN group than in T2DM group. SUA were significantly increased together with the increase of the severity of DPN [(279.66 ± 56.53)vs(308.93 ± 52.96)vs(330.24 ± 48.79) μmol/L](P<0.05). Conclusion SUA level is an risk factor for the development of DPN in the middle‐aged and elders with T2DM. SUA is positively correlated with the severity of DPN.

Objective To investigate the expression levels of miR-146a in the peripheral blood mononuclear cells ( PBMCs ) of patients with diabetic peripheral neuropathy ( DPN ) and their correlation with the disease severity. Methods The expression levels of miR-146a in the PBMCs were measured by using real time PCR in 62 patients with type 2 diabetes (32 patients without DPN, 30 with DPN) and 33 healthy individuals. The correlation of miR-146a expression with clinical parameters was analyzed. Results The expression level of miR-146a in patients withtype2diabetesmellituswassignificantlylowerthanthatinthehealthyindividuals((1.92±1.99vs4.15±1.56, P<0. 05). Furthermore, the miR-146a level was significantly lowered in diabetic patients with DPN as compared with thosewithoutDPN(1.22±1.61vs2.51±2.00,P<0.05). TheexpressionlevelsofmiR-146ainpatientswithDPN were positively correlated with duration of diabetes, blood glucose, and Toronto clinical scoring system scores. Conclusion The decreased expression of miR-146a in patients with DPN is correlated with the severity of DPN.

Objective To explore the associations between polymorphisms of Vitamin D receptor gene and type 1 diabetes susceptibility. Methods Literatures were retrieved from PubMed ,Web of Science and WanFang databases ,etc.Pooled odds ratios(ORs) with 95% confidence intervals (CIs) were calculated using a random effect model. Results A total of 28 literatures were included. The result of analysis showed that BsmI and ApaI polymorphism were the susceptibility gene for T 1DM in Asian populations [B vs b;OR(95% CI)=1.53(1.06~2.20) ,P=0.024 ;AA vs aa:OR(95% CI)=1.60(1.06~2.40) ,P= 0.023]. Conclusion The BsmI and ApaI polymorphism may be susceptibility gene in Asians populations with T1DM.

Objective To observe the real-time efficacy of balance acupuncture in treating primary hypertension. Method Totally 160 patients with primary hypertension were randomized into a treatment group and a control group, 80 in each group. The treatment group was intervened by balance acupuncture, while the control group was by oral administration of Captopril. The blood pressure and symptom score were observed before and after intervention. Result There were no significant differences in comparing the total effective rate at different time points between the two groups (P>0.05). There was a significant difference in comparing the headache score in male patients at 30 min after treatment between the two groups (P<0.05). Conclusion Balance acupuncture is an effective method in treating primary hypertension, and can release headache of the patients.

[Objective] To observe the effect of electroacupuncture (EA) on the disorder of learning and memory in rats with vascular dementia (VD) and to explore its molecular mechanism. [Methods] Among 60 male SD rats, 10 rats were in sham-operation group and the other 50 rats were used to establish models of global ischemia and reperfusion by four-vessel occlusion (4-VO) method. Then the survival model rats were randomized into EA group (n = 14) , model group (n = 13) and nimotop group (n = 13). EA group was treated with EA at points of Baihui (GV20) and Dazhui (GV14) , nimotop group with nimotop 12 mg/kg, and sham-operation group and model group did not receive any treatment. After a 20-day treatment, the changes of learning and memory behavior in rats were detected by Morris water maze, as well as the protein expression and mRNA expression of heme oxygenase (HO) . [Results] The escape latent period, HO-1 protein expression and HO-1 mRNA-expression were increased obviously in model rats ( P 0.05). [Conclusion] EA can improve the learning and memory behavior in rats with VD and this may be related to the decrease of HO-1 protein expression and HO-1 mRNA-expression in cortex and hippocampal neurons.

Objective To evaluate the transcutaneous oxygen pressure (TcPO2 )and its clinical significance in patients with type 2 diabetic peripheral neuropathy (DPN). Methods A total of 222 hospitalized patients with type 2 diabetes mellitus (T2DM)from the Department of endocrinology of Lianyungang First People's Hospital in Jiangsu Province were enrolled in this study from October 2014 to September 2015.And 50 healthy controls were also selected in this study as control group (NC group). TcPO2 was tested in supine position in all the subjects by TCM400 TcPO2 detector. Patients with T2DM then were divided into two groups according to Nerve conduction velocity (NCV):DPN group (n = 102) and non-DPN group (NDPN group,n = 120).Anthropometric and metabolic parameters were assessed in each group. Results TcPO2 in supine positionwas lower in DPN group than in NC and NDPN group. TcPO2 was positively correlated with diabetes duration,TCSS scores,HbA1 c,SUA,FPG and NCV.Compared with normal TcPO2 group,the prevalence of DPN was increasedin low TcPO2 group,while NCV of motor nerve and sensory nerve were decreasedin low TcPO2 group.Logistic analysis showed that TcPO2 was an independent risk factor for DPN. Conclusion TcPO2 in supine position was decreased in patients with DPN,and is a risk factor for DPN,which may provides valuable information for DPN diagnosis.

Objective To explore the possible role of miR-146a in the pathogenesis of diabetic peripheral neuropathy( DPN) by investigating the correlation of miR-146a level in the peripheral blood mononuclear cells (PBMCs)with tumor necrosis factor α(TNF-α), interleukin-6(IL-6), and C reactive protein(CRP). Methods The expression levels of miR-146a in the PBMCs were measured using realtime PCR in 81 patients and 34 healthy individuals. The correlation of miR-146a expression with clinical parameters was analyzed. Results Compared to those in the healthy individuals and patients without DPN, miR-146a level was significantly decreased in diabetic patients with DPN while TNF-α, CRP, IL-6, HbA1C, systolic blood pressure, and homeostasis model assessment of insulin resistance index(HOMA-IR) were significantly increased(all P<0.05). Duration of diabetes, blood uric acid, serum creatinine, and urinary albumin to creatinine ratio in diabetics with DPN were higher compared with diabetics without DPN(all P<0. 05). The expression level of miR-146a was negatively correlated with diabetes duration, HOMA-IR, HbA1C, TNF-α, and IL-6. Multiple linear regression showed that HOMA-IR, HbA1C, TNF-α, and IL-6 were independent factors of miR-146a. Conclusion miR-146a may be involved in the development of DPN through inflammation, glucose metabolism, and insulin resistance.

Objective To observe the effect of electroacupuncture(EA) on long-term potentiation(LTP),calmodulin(CaM) and calmodulin protein kinase Ⅱ(CaMPKⅡ) mRNA expression in vascular dementia rats.Methods Seventy SD rats,half in male and aged 2 months,were randomized into 5 groups: normal group(N =14),pseudo-operation group(N = 14),EA group(N = 12),western medicine group(N = 12),and model group(N =12).EA group were given acup uncture on Baihui(GV20),Dazhui(GV14) and Shenshu(BL23),and western medicine group were given nimoldipine 12 mg.kg-1.d-1.The normal group,model group and pseudo-operation group were fed routinely without any medication.Escape latency and frequency of rats crossing the quadrant of the platform in Morris water maze,in-vivo hippocampal LTP,and neuronal CaM and CaMPKⅡ mRNA expression were observed.Results Compared with the model group,escape latency was shortened,frequency of rats crossing the original and left quadrants of the platform rised,LTP amplitude and duration increased,and neuronal CaM and CaMPKⅡ mRNA expression enhanced in EA group and western medicine group(P

Objective To investigate the relationship between CA199 and clinical indicators and its related factors in patients with type 2 diabetes mellitus(T2DM). Methods A total of 1224 hospitalized T2DM patients were enrolled retrospectively from the Endocrinology and metabolism department of our hospital. All the patients were divided into two groups according to CA199 level:negative group (CA199<37 U/ml) and positive group(CA199≥37 U/ml). Clinical and biochemical data were compared between the two groups. Results The level of FPG,HbA1c,UACR,and the prevalence of diabetic microvascular complications were significantly higher in CA199 positive group than in negative group[(13.12±4.58)vs(10.22±4.01)mmol/L,(10.84±2.58)% vs (8.78±2.22)%,(38.25±3.15) vs (29.09±1.02)mg/g,P<0.05]. Serum albumin level was lower in CA199 positive group than in negative group[(38.59±5.33) vs (40.13±5.63)g/L,P<0.05]. Pearson correlation analysis showed that CA199 was associated with FPG,HbA1c,and serum albumin(r=0.251,0.260,-0.082,P<0.05 or P<0.01). Multiple logistic regression analysis further found that FPG and HbA1c were the independent risk factors for CA199(β=0.793,0.975,P<0.05). Conclusion The elevated CA199 in T2DM patients correlated with poor glucose control and development of diabetic microvascular complications.

Objective To assess the efficacy of postoperative adjuvant interferon (IFN) therapy on patients with hepatitis B virus (HBV) related hepatocellular carcinoma (HCC).Methods An electronic search for articles published from January 2000 to January 2013 was conducted to identify English language comparative studies evaluating IFN therapy on recurrence and survival after surgical treatment of HCC.Results A total of five trials consisting of 694 patients were included in the Meta-analysis.The estimated odds ratios (OR) for the 1-,2-,3-,and 5-year overall survival rates of HBV-related HCC were 3.37 (95％CI:1.18-6.27,P=0.000),2.36 (95％ CI:1.45-3.83,P=0.001),1.81 (95％ CI:1.21-2.72,P=0.004),and 1.93 (95％ CI:1.35-2.75,P=0.000),respectively.The OR for the 1-,2-,3-,and 5-year recurrence rates were 0.63 (95％ CI:0.44-0.91,P=0.014),0.84 (95％ CI:0.60-1.18,P=0.322),0.88 (95％ CI:0.63-1.22,P=0.431),and 0.78 (95％ CI:0.56-1.07,P=0.120),respectively.Conclusion This Meta-analysis shows that IFN therapy had a significant clinical effect in improving overall survival rates but not in decreasing recurrence rates of HBV infected HCC patients postoperatively.