1.Molecular characteristic analysis of E gene in genotype 3 Japanese encephalitis virus in China
Chinese Journal of Microbiology and Immunology 2010;30(5):387-391
Objective To identify the molecular characteristics on nucleotide and amino acid in E gene through the comparative analysis of differences between genotype 3 Japanese encephalitis virus(JEV)in China and the live attenuated vaccine(SA14-14-2).Methods Obtained the E gene nucleotide sequence of Japanese encephalitis virus from GenBank.Multiple alignment and amino acid(AA)analysis were carried out by Clustal X(1.81),DNAStar and GENEDOC(3.2).According to the ribbon diagram of the E protein structure based on the model of the soluble fragment of tick-borne encephalitis virus(TBEV)as a template for Japanese encephalitis virus E protein amino acid sequence analysis.Results Compared different regions and different host genotype 3 JEV with SA14-14-2 the nucleotide sequence homology was,up 96%and 95% and the amino acid homology was up 95%and 94%,respectively.The nucleotide and amino acid homology was higher among the same area and the same host.Ten common amino acid variations and five special variations(E160 in domain Ⅰ,E123 and E227 in domain Ⅱ,E441 and E487 in non-structural domain)were found between genotype 3 JEV and SA14-14-2.Conclusion The homology in E gene was higher between genotype 3 JEV in China and SA14-14-2.Five special amino acid differences were found in genotype 3 JEV.The existing live attenuated vaccine strain is theoretically possible to protect genotype 3 JEV in China.
2.Epidemiological analysis of gastric cancer and esophagogastric junction cancer
Guodong LI ; Jie LIANG ; Qinghua ZHAI
Cancer Research and Clinic 2017;29(7):500-504
In the past decades, epidemiological trends of the gastric cancer and esophagogastric junction (EGJ) cancer have changed all over the world. The morbidity and mortality of gastric cancer is on the decline, while the incidence and mortality of EGJ cancer is on the rise. This article summarizes the pathological types and the descriptive epidemiology, including the incidence, mortality, survival rate and its tendency in different gastric cancers and EGJ cancer. It also discusses the influencing factors of gastric cancer and EGJ cancer including environmental and genetic factors, which provides new ideas for the further study of the two kinds of cancers.
3.West Nile virus and its human and animal infections in China field surveys and laboratory investigations
YIN Qikai ; WANG Huanyu ; LIANG Guodong
China Tropical Medicine 2024;24(1):28-
The West Nile virus (WNV), a mosquito-borne arbovirus, is also a zoonotic pathogen first isolated in the 1930s in Africa, followed by the identification of the prevalence of febrile illness caused by West Nile virus infections. In 1999, the West Nile virus was first introduced into New York City of the United States, and caused the outbreak of viral encephalitis in adults. This marked the first reported outbreak of mass adult viral encephalitis caused by West Nile virus. Subsequently, West Nile virus and its infections in humans and animals spread rapidly throughout the United States, causing a worldwide sensation. West Nile virus is currently considered the most widely distributed emerging mosquito-borne arboviruses worldwide. Humans or animals infected by mosquito bites can develop symptoms such as fever, encephalitis (meningitis), and in rare cases, present with severe pancreatitis, hepatitis, myocarditis, miscarriage, or even death, posing a huge global public health burden. This review introduces China's progress in the isolation and identification of West Nile virus, the prevalence of adult viral encephalitis, and the field surveys and laboratory investigations of the coinfection of West Nile virus and typhoid bacteria, aiming to promote the research work and control and prevention of West Nile virus and its infection in China.
4.The influence of ShengJi-hongfen Cream on wound healing histology and bFGF content of rabbits
Zifu HONG ; Guodong LI ; Liang YUAN ; Yinghua HE
International Journal of Traditional Chinese Medicine 2010;32(3):203-204
Objective To investigate the mechanism of Shengji-hongfen Cream(SJHFC)affecting basic fibroblast growth factor(bFGF)in wound and hastening wound healing.Methods Rabbit models of cutaneous deficiency and infection were setup.Self-contrast observation was used for comparing the two wound sides of model rabbit,which were administrated with SJHFC(treatment group)and vaseline(control group)respectively.Microstructure of wound surface and changes of bFGF weTe observed and measured.Results The content of fibroblasts,phlogocyte,collagen and blood vessel in the SJHFC group increased markedly,compared with the control group after one week.The content of bFGF was significantly changed than the control group at the 3rd and 7th day(P<0.05).Conclusion SJHFC Can promote bFGF content of the wound,that maybe the mechanism of its hastening wound healing.
5.Application of Journal club teaching method in continuous education for neurosurgical refresher doctors
Liang HENG ; Jipei ZHAO ; Lijun HENG ; Guodong GAO ; Dong JIA
Chinese Journal of Medical Education Research 2012;(11):1127-1130
Tangdu hospital of the fourth military medical university explored the aim,method,critical points and effect for organizing a neurosurgical continuous education Journal club and summarized the key factors important for its successful organization and the improvement of its short-term and long-term educational effects in order to discover a Journal club education pattern which could satisfy the demand of for neurosurgical refresher doctors.Paying attention to these factors could better satisfy the demand of for neurosurgical refresher doctors,achieve their participating aims,promote their understanding of the advancing front of the discipline and improve their literature analyzing competence and problem solving ability.The wide application of continuous education Journal club is helpful to improve the level of neurosurgical continuing education in our country.
6.Whole-genome sequencing on one case of Han familial keloids.
Guodong TENG ; Minliang CHEN ; Chang LIU ; Liming LIANG
Chinese Journal of Plastic Surgery 2016;32(1):52-55
OBJECTIVETo screen SNP information of keloid pedigrees through whole genome sequencing.
METHODSWe Collected information and clinical data of the keloid pedigree and constructed charts of the pedigree. The DNA was extracted from peripheral venous blood samples of the pedigree to sequence the whole genome.
RESULTS27 SNP and 8 disease-associated genes were screened out.
CONCLUSIONSWhole genome sequencing technology can select new genetic mutations associated with keloid, and provide a new way for the research of keloid.
China ; ethnology ; Genome, Human ; Humans ; Keloid ; genetics ; Mutation ; Pedigree ; Polymorphism, Single Nucleotide ; Sequence Analysis, DNA
7.Role of the E gene in the infectivity of SINV and SINL in infected cells
Lihua WANG ; Shihang FU ; Wuyang ZHU ; Qing TANG ; Guodong LIANG
Chinese Journal of Microbiology and Immunology 2010;30(5):410-415
Objective To elucidate the molecular basis on the differences of infectivity in infected cells between Sindbis virus(SINV:YN87448 virus)and Sindbis like virus(SINLV:XJ-160 virus).Methods Compare the E(glycoprotein)gene sequence and secondary structure of YN87448 virus and XJ-160 virus by bioinformatics analysis.Analyze the contribution of E gene to the biological differences between SINV and SINLV by constructing recombinant virus.Results By bioinformatics analysis,YN87448 virus and XJ-160 virus have the same genomic structure,which has 11 717 nt and 11 626 nt respectively.There are 82 amino acid differences between E gene of these two viruses,and showed scattered distribution.The main peak is basically the same for the hydrophobic of the E gene protein,but in some region existing small differences.The recombinant virus which exchanged the E gene of XJ-160 virus with YN87448 virus totally showed the biological character of YN87448 virus,either in the showing time of CPE,plaque forming time and plaque diameter,or in expression of functional proteins.Conclusion E gene plays a major role in the differences of infectivity in infected cells between SINV and SINLV,this result provide the molecular biological evidences for elucidating the biological differences between SINV and SINLV.
8.Clinical characteristics and genetic survey on four families of keloid
Guodong TENG ; Minliang CHEN ; Chang LIU ; Tongtong YAN ; Liming LIANG
Chinese Journal of Medical Aesthetics and Cosmetology 2015;21(2):91-94
Objective To study the clinical and genetic characteristics of keloid through investigating on four Han Chinese pedigrees.Methods The pedigree information and clinical data from Han Chinese keloid pedigrees were collected,which consisted of 22 patients in 127 family members,and then the charts of these pedigrees were constructed according to the data.Using the genetic model and pedigree analyses we summarized the clinical features of the disease in the families.Results Four Han Chinese keloid pedigrees were discovered.The three pedigree spans included 3 generations and one was 4 generations.Incidence of KD in the consanguinity family member was 23.7% (23/93),and 20.8% (11/53) in male KD,and 27.5% (11/40) in female.Incidence of anterior chest KD was 40.9 %.The inheritance pattern observed in these pedigrees was consistent with an autosomal dominant inheritance multi-gene hereditary disease with incomplete penetrance,and its nonpenetrance of KD gene carriers was 12% (3/25).Conclusions The pattern of inheritance observed in these four Han Chinese keloid pedigrees is similar to previous reports and no gender differences are found in the incidence of disease,but differences in pathogenic site.Pedigree investigation helps to reveal the genetic characteristics of keloid.
9.Expression and significance of COX-2 protein and BCL-2 protein in distal transitional mucosa adjacent to rectal carcinoma
Guodong PANG ; Dongfeng ZHOU ; Yang LI ; Yibo LIANG ; Lin CUI
Chinese Journal of Current Advances in General Surgery 2011;14(1):55-58,70
Objective:To detect the expressions of COX-2 protein and BCL-2 protein in transitional mucosa adjacent to rectal carcinoma,and determine whether the transitional mucosa was the cancer precursor event.Methods:Mucin histochemical method was employed to detect the distal mucosa 2 cm away from rectal tumor and the transitional mucosa was found in 54 cases of rectal carcinoma.Immunohistochemical method was used to investigate the expressions of BCL-2 and COX-2 protein in the specimen of rectal carcinoma mass,transitional mucosa and non-transitional mucosa,and 20 cases of normal rectal mucosa,and the points of the expressions of COX-2 protein and BCL-2 protein were counted.Results:35.19%(19/54)of distal mucosa were characterized as the transitional mucosa.The expressions of COX-2 and BCL-2 protein were detected in carcinoma mass and distal mucosa.Significant difference was observed in carcinoma mass and transitional mucosa(p<0.01).But there were no significant differences between the expressions of those in transitional mucosa and non-transitional mucosa(p>0.05),as well as transitional mucosa and normal mucosa(p>0.05).Conclusion:The performance of transitional mucosa was not the cancer precursor event,but the non-specificity reaction of carcinoma or inflammation.
10.Simulation and control in the process of concentration ——the Simulation of the change of boiling point in the process of concentrate
Liang HUANG ; Guodong WANG ; Liguo ZHANG ; Liju NI
Chinese Traditional Patent Medicine 1992;0(02):-
AIM: To observe the change of boiling point in the concentration of medical herb's extract liquor. METHODS: Extract liquors of typical herb. such as Radix salviae Miltiorrhizae, Flos chrysanthemi indici, Folium isatidis and Radix Astragli, were concentrated in the normal pressure, its boiling-points and mol concentrations were recorded and its curve was depicted. RESULTS: At the same concentration, the boiling points of different kinds of Chinese medicinal material is different. CONCLUSION: In the concentration process of distillation the change of solvent and solute should be taken into account.