BACKGROUND:Most orthopedic surgeries use metal implants. Although this approach wil benefit for patients, it simultaneously makes patients infected by some bacteria. Serum C-reactive protein level and erythrocyte sedimentation rate are two indices for clinical y determining the inflammation. OBJECTIVE:To investigate the changes in serum C-reactive protein level and erythrocyte sedimentation rate in patients after use of orthopedic metal implants. METHODS:The clinical data of 100 patients who used metal implants between October 2012 and October 2014 at the Department of Orthopedics, Linqu People’s Hospital of Shandong Province of China were retrospectively analyzed. Among these patients, 36 patients received implantation of intramedul ary nails, 34 patients artificial joint, and 30 patients steel plates. At 1, 3, 7, 14, and 21 days after implantation, infection was determined according to serum C-reactive protein level and erythrocyte sedimentation rate, as wel as other laboratory indices, wound healing, and body temperature. According to these indices, the patients were assigned to two groups:no infection (n=52) and infection (n=48). Disease progression was analyzed for providing reliable evidence for rational use of antibiotics. RESULTS AND CONCLUSION:There were significant differences in serum C-reactive protein level and erythrocyte sedimentation rate in patients between infection and non-infection groups at 1 (t=3.43, P<0.05;t=3.78, P<0.05), 3 (t=3.35, P<0.05;t=3.68, P<0.05), 7 (t=3.28, P<0.05;t=3.54, P<0.05), and 14 days after use of orthopedic metal implants (t=2.56, P<0.05;t=3.02, P<0.05). No significant differences in these two indices were observed between infection and non-infection groups at 21 days after use of orthopedic metal implants (t=1.76, P>0.05;t=1.98, P>0.05). These findings suggest that after use of orthopedic metal implants, serum C-reactive protein level and erythrocyte sedimentation rate in patients should be closely monitored and simultaneously other indices like wound healing and body temperature should be determined to determine whether infection occurs after use of orthopedic metal implants so as to develop effective repair strategies. This wil provide reliable clinical evidence for rational treatment and prevention of orthopedics-related diseases.

Objective To establish the rat ischemia-reperfusion injury model and learn the rat apoptosis changes after ischemia-reperfusion injury.Methods Ligate rat left common carotid artery 1h,reperfuse and exam retinal apoptosis in 1,6,12,24,48 and 72h groups.Result Apoptosis mainly occurs in retina ganglion cell layer and inner nuclear layer,occasionally in outer nuclear layer.Apoptosis occurs in 6h after reperfusion.In 24h,apoptosis achieves the peak,then decrease gradually.However,in 72h after reperfusion,there is apoptosis in rat retina.Conclusion rat retina ischemia-reperfusion injury can be observed after ligating common carotid artery.Apoptosis may play an important role in rat retina ischemia-reperfusion injury.In 72h after the operation,the injured retina didn't recover completely.

Objective To evaluate the efficacy and safety of dexmedetomidine combined with propofol for gastroscopy in elderly patients.Methods Ninety ASA physical status Ⅰ-Ⅲ patients,aged 65-80 yr,weighing 45-80 kg,scheduled for elective gastroscopy,were randomized to receive either dexmedetomidine combined with propofol (group DP,n =45) or propofol (group P,n =45).In group DP,dexmedetomidine 0.4 μg/kg was infused over 5 min and propofol 1.0 mg/kg was injected intravenously 25 min later.Propofol 2.0 mg/kg was injected intravenously in group P.When body movement occurred during operation,additional propofol 0.2 mg/kg was given intermittently.Mean arterial pressure (MAP),heart rate (HR),pulse oxygen saturation (SpO2) and Narcotrend index were monitored during the whole procedures.The consumption of propofol and vasoactive agents,adverse cardiovascular events,and respiratory depression were recorded.Results Compared with group P,the consumption of propofol,incidences of respiratory depression and tachycardia,and degree of body movement were significantly decreased (P ＜ 0.05),Narcotrend index was increased during operation (P ＜ 0.01),and no significant change in the incidence of circulatory depression was found in group DP (P ＞ 0.05).Conclusion Small dose of dexmedetomidine 0.4 μg/kg combined with small dose of propofol 1.0 mg/kg provides good efficacy and safety for gastroscopy in elderly patients.

Mental matching is a strategy of teaching materials transacting in emotional latitude of teaching psychology. Used properly, psychology matching strategy can improve the unification between teaching materials and students' need to adjust to the students' learning interests. This paper introduces the connotations and functions of mental matching strategy, and two types of mental matching strategies in the nursing ethics teaching are analyzed.

Adult ; Aged ; Electroacupuncture ; Fecal Incontinence ; etiology ; therapy ; Female ; Humans ; Male ; Middle Aged ; Postoperative Complications ; etiology ; therapy ; Rectal Prolapse ; surgery ; Young Adult

Objective To evaluate the effect and safety of point injection and em bedding therapy on postoperative pain of hemorrhoid patients. Me thods Totally 105 hemorrhoid patients were rand omized into a treatment group (54 cases) and a control group (51 cases). The tre atment group was treated by point injec tion and embedding therapy at Changqiang point (GV 1) under the local anesthesia . The control group was treated by Indometacin Suppository after operation. On the fir st, second, four th and seventh day of the treatment, the pain occurrence, pain severity, and com prehensive effect scores were recorded for the evaluation of therapeutic effect and safety. Results On all interview periods, the compr ehensive effect score, pain occurrence, pain severity, patient satisfaction, occurrence of untoward eff ect, complications, and analgesics combination in the treatment group were all superior to those in the control group (P

AIM: To observe the effect of cannabinoid receptor (CB1R) agonist WIN55-212-2 on the expression of brain-derived neurotrophic factor (BDNF), c-Fos and protein kinase A beta-catalytic subunit (PKAC-β) in cerebrum cortex after intracerebral hemorrhage (ICH) in rats. METHODS: The intracerebral hemorrhage model of rat was made by the injection of collagenase Ⅶ, and WIN55-212-2 was intraperitoneally (ip) injected 30 min later. The rats were killed for sampling the brain tissues as specimens 24 h after ICH. The methods of immunohistochemical analysis and Western blotting were adopted to detect the expression of PKAC-β and BDNF. The mRNA expression of PKAC-β, c-Fos and BDNF was determined by RT-PCR. RESULTS: WIN55-212-2 obviously improved some nervous deficit symptoms and increased the expression of BDNF at mRNA and protein levels with upregulating the mRNA expression of c-Fos and downregulating the expression of PKA at mRNA and protein levels in the ipsilateral cerebral cortex. The proteins of PKAC-β, c-Fos and BDNF were expressed on the membrane or nucleus of the neuron or in the cytoplasm of glial cells, respectively. CONCLUSION: The expression of BDNF is induced not only by upregulation of c-Fos, but also by downregulation of PKA in WIN55-212-2 treated rats.

BACKGROUND: Mesenchymal stem cells (MSCs) are commonly observed under the scanning electron microscope, transmission electron microscope and inverted microscope. However, above-mentioned observation methods have disadvantages on observing ultrastructure of cell membrane and cytoskeleton. OBJECTIVE: To establish a more effective and appropriate method to isolate, culture and identification of human umbilical cord mesenchymal stem cells (hUCMSCs) in vitro and to study the ultrastructure of osteogenic differentiation with the atomic force microscope. METHODS: The hUCMSCs were isolated from human umbilical cord by digested with collagenase. After serial subcultivation in vitro, the stem cells were passaged, and osteogenic differentiation was determined by alkaline phosphatase calcium-cobalt staining and alizarin red staining. hUCMSCs immunophenotype was measured by Flow cytometry.The membrane surface ultrastructure of osteogenic differentiation was observed by Atomic Force Microscope before and after induction. RESULTS AND CONCLUSION: The isolated hUCMSCs by digested with collagenase was efficient. After seeded 24 hours, the adherent cell showed spindle shape, polygonal shape and fibroblast-cell-like shape and the size of hUCMSCs was homogeneous. Flow cytometry analysis revealed that CD29, CD44, CD105 were highly expressed on the surface of passages 3 cells, but there was negative for CD34, CD45 and HLA-DR. These cells were high positive for alkaline phosphate staining and also showed significant calcium node using alizarin red staining after 4 weeks culture induction of osteogenic differentiation. Under atomic force microscopy, undifferentiated stem cells demonstrated insignificant microtubule protrution in a parallel distribution following analysis of cytoskeleton before and after differentiation.

Objective To compare the clinical efficacy of seretide (50 μg/500 μg salmeterol/fluticasone propionate) with seretide (50 μg/250 μg salmeterol/fluticasone propionate) in single inhaler in the treatment of patients with stable status moderate to severe chronic obstructive pulmonary disease (COPD)Methods Sixty patients with COPD were randomly divided into the treatment and the control groups.Baseline treatments were similar in all patients,patients in the treatment group received seretide (50 μg/500 μg) while the control group received seretide (50 μg/250 μg) inhalation once every 12 hours for 24 weeks Before and after the therapeutic course,tests for lung function in patients of the two groups were conducted and compared with each other.Clinical symptoms and physical signs were graded by questionnaire.Results There was no significant difference on indexes of lung function between the two groups at baseline (P ＞ 0.05).After treatment,the score of clinical symptoms and signs in the treatment group was lower than that in the control group ((4.0 ± 0.5) vs.(4.8 ± 0.3),t =2.63,P ＜ 0.05).Six minutes walking distance was longer in the treatment group than that in the control group ((451.6±22.9) meter vs.(401.2 ±25.4) meter,t =2.51,P ＜0.05).The levels of forced exhaled gas volume 1 (FEV1),FEV1/forced vital capacity (FVC) and FEV1/pred in the treatment group were higher than those in the control group ([FEV1:(2.18 ± 0.38) L vs.(1.78 ± 0.45) L;FEV1/pred:(63.19 ±9.08)％ vs.(57.19 ±9.25)％; FEV1/FVC％:(73.8 ±5.6)％ vs.(67.3 ± 11.5)％ ;P ＜ 0.05).Conclnsion High dosage of seretide had better effect in the treatment of stable moderate and severe COPD,and can obviously improve patients' lung function,clinical symptoms and quality of life.

early lithagogue rate.