1.Study the Variety of Serum Interleukin-2 Receptor, Interleukin-8, Immunoglobulin, T-lymphyocyte Subgroup in Patients with COPD
Kaifen YUAN ; Guohou ZHAO ; Jianhua YANG ; Guocui ZHANG
Journal of Kunming Medical University 2001;22(1):59-60
To study the variety of sIL-2R, Il-8, immunoglo bulin, T-lymphycyte subgroup in patients with COPD. 30 cases with COPD and the control group of 30 cases were d etected sIL-2R, IL-8 with ELISA; IgG, IgA with rate nephelometry; CD+3,CD+1, Cd+ 3,with immunoflu orescence. Results: Patients with CO-PD sIL-2R, Il-8, CD 8 increase and IgG, IgA, CD+3, CD+4,CD+4/CD+8 decrease . The difference is very significant. Conclusion: The immuni faction of patients with COPD reduce.
2.The effect of glucocorticoids in patients with acute exacerbation of severe COPD and its impacts on the serum levels of matrix metalloproteinase 9 and tissue inhibitor of metalloproteinase-1
Peifang ZHANG ; Zhiyang LUO ; Lixian ZHENG ; Zhanqiang MEI ; Guocui ZHEN
Clinical Medicine of China 2010;26(1):38-40
Objective To explore the role of matrix metalloproteinase 9(MMP-9)and tissue inhibitor of metalloproteinase-1(TIMP-1)in patients with acute exacerbation of severe chronic obstructive pulmonary diseases (AECOPD)treated with glucocorticoids.Methods The serum levels of MMP-9 and TIMP-1 were measured by ELISA in 41 patients with AECOPD before and after treatment with glucocorticoids and in 40 patients with regular methods(control group).Results ①After treatment with glucocorticoids,the serum levels of MMP-9(189.25±52.38)μg/L、TIMP-1(198.38±43.45)μg/L,ratio of MMP-9/TIMP-1(0.92±0.37)were decreased significantly than before((246.10±68.64)μg/L,(217.63±62.34)μg/L,(1.09±0.23))(P<0.01,P<0.05,P<0.05);but there were no differences(P>0.05)in regular methods(control group);② After treatment with glucoeorticoids, FEV_1、FEV_1%((0.83±0.35)L,(46±17)%)were impmved than before((0.72±0.48)L,(34±15)%)(P<0.05).Conclusions After treatment with glucocorticoids,the serum levels of MMP-9、TIMP-1、ratio of MMP-9/TIMP-1 from patients with COPD were decreased significandy,and FEV_1、FEV_1%were improved significant;Glucocorticoids can alleviate severs AECOPD to some degrees,which might be related to improving the imbalance of MMP-9/TIMP-1.
3.Protective effect of extract of astragalus against injury induced by hypoxia/reoxygenation in hippocampus neuron
Fenfang ZHU ; Yanyan YIN ; Weiping LI ; Weizu LI ; Guocui WU ; Huilin GONG ; Wen ZHANG
Chinese Pharmacological Bulletin 1987;0(02):-
Aim To study the effect of EA on the injury induced by hypoxia/reoxygenation in primary cultures of rat hippocampal neurons.Methods Rat hippocampal neurons in primary culture were used,and a apoptosis model was induced by hypoxia/reoxygenation.MTT assay and LDH releasing rate were used to detect the cell viability.The apoptosis rate of hippocampal neurons was analyzed by Hoechst 33258 staining,flow cytometry with AnnexinV-FITC and PI staining.Western blot was used to detect the protein expression of AKT and p-AKT.Results Compared to control group,three hours of hypoxia followed by sixteen hours of reoxygenation induced hippocampal neuronal apoptosis.EA could raise the neuronal viability and reduce apoptosis rate and the damage degree of rat hippocampal neurons.EA could increase the expressing of p-AKT.Conclusions EA has protective effects on damaged neurons,and the mechanism may be related to activating the PI3K-AKT signal transduction pathway.
4. Inhibition of CD4+T cell infiltration by interleukin-10 competent B cells in periodontitis tissues
Guoqin CAO ; Xu ZHANG ; Yan ZHAO ; Siqi ZHAO ; Guocui DONG ; Qiuxiang GAO ; Zuomin WANG ; Jiang LIN
Chinese Journal of Stomatology 2019;54(8):553-560
Objective:
To study the immune regulation function of high expressing interleukin-10 (IL-10) in B cells on CD4+T-cells in periodontitis mouse model.
Methods:
Twenty-four 7-weeks-old female C57BL/6 mice were randomly and equally assigned into 4 groups: the healthy control group (HC group,