In order to improve rational useage of inerferon in clinic,this article elaborate the background of this Specialists consensus on pediatric clinical application of recombinant human interferon-α1 b,the differences of various subtypes of interferon,the envidence and method of interferon-α1b clinical application,the satety and the adverse reaction monitoring issues in this article.

OBJECTIVE: To investigate the effects of Baobaole oral liquid on neuronal excitability in lateral hypothalamic area (LHA) and ventromedial hypothalamic nuclear (VMN) in anorectic rats. METHODS: The anorectic rat model was established by feeding with special prepared forage for a week, and then Baobaole oral liquid, a liquid extract of a compound traditional Chinese medicine for activating spleen, was administered once a day for 3 weeks. Finally, extracellular recording from LHA and VMN neurons in rats were made in order to characterize their responses to gastric vagal nerve stimulation and intravenous injection of glucose in the normal, untreated, and Baobaole-treated groups. RESULTS: There was no statistical difference in response characteristics of LHA neurons to gastric vagal stimulation among 3 groups. The duration of VMN neuron excitation response to gastric vagal nerve stimulation in the untreated group was significantly longer than that of the normal control group (P<0.01), while the required stimulation intensity was significantly decreased (P<0.01). Moreover, among the neurons responding to the gastric vagal stimulation in the untreated group, the number of glycemia-sensitive neurons decreased in LHA and increased in VMN (P<0.01). The gastric vagal stimulation induced neuron responses in LHA and VMN of the Baobaole-treated group were not significantly changed as compared with the normal control group (P<0.01), and neither were the intravenous injection of glucose induced responses. CONCLUSION: Baobaole oral liquid can modulate the sensitivity of LHA and VMN neurons to the peripheral signal and make the coordination between LHA and VMN neurons in order to improve the appetite of anorectic rats.

OBJECTIVE：To compare the effect and adverse reactions of clarithromycin(Limaixian，CT)with those of erythromycin(ET)in treating respiratory tract mycoplasmal infections of children.METHODS：98 patients were randomly divided into CT group(56 cases)and ET group(42 cases)，CT in oral dosage of 20mg/(kg.d)and ET in iv dosage of 30～40mg/(kg· d)were given respectively.RESULTS：The curative rate，effective rate and clearance of mycoplasma of CT group were higher than those of ET group with significant differences(P<0.05).The adverse reactions induced by oral CT were less than those by iv infused ET(P<0.01).CONCLUSION： Oral CT shows satisfactory therapeutic effect， less adverse reactions and can effectively clear away mycoplasma in treatment of respiratory tract mycoplasmal infections of childhood.

Objective To investigate the correlationship of second-trimester serum human chorionic gonadotropin (hCG) level and preeclampsia, premature rupture of membranes and preterm delivery. Methods 200 cases of second-trimester gestational serum hCG level was determined by radioimmunoassay,and the pregnancy outcome was followed up. Results The maternal serum hCG level in every gestative week has no significant differences;hCG level of pregnancies with preeclampsia,premature rupture membranes and preterm delivery was significantly higher than normal pregnancies. The rate of developing preeclampsia,premature rupture of membranes and preterm delivery in those hCG ≥2MOM patients (30.9%、35.8% and 14.8% respectively)was significantly higher than those MshCG

0.05);While the differences of the levels of p53,Bcl2,Bax between all D_1+D_2 groups and sham-irradiated group were significant(P

Objective:To study the conditions of in vitro proliferation and differentiation of rat bone marrow stromal cells(MSC) for its application in transplantation.Methods:MSC were obtained by flushing the shaft with DMEM using a sterile syringe,dissociated by density gradient centrifuge,and cultured in the medium containing DMEM and 10% fatal bovine serum(FBS).Then we added neural growth factors into the medium and observed their influence on MSC proliferation,differentiation and survival by phase contrast microscopy.Immunocytochemistry was used to identify cell types.Results:MSC cultured in medium containing DMEM/ 10% FBS/ neural growth factors proliferated quickly.The neuron-like cells appeared at 7-9 d with the expression of neurone specific enolase(NSE) at(52?9.2)% and glial fibrillary acidic protein(GFAP) at(15?7)%,and could survive more than 7 days;In contrast,cells induced by ?-mercaptoethanol(BME) died gradually after 24 hours.Conclusion:MSC can not only proliferate and differentiate into neuron-like cells effectively in the medium containing neural growth factors but also survive longer.

Objective To approach the status of leprosy disabilities in newly detected cases in recent 10 years in China and provide the scientific basis for formulating the preventive strategies. Methods Based upon the individual records from the National Leprosy Recording and Reporting System in National Center for STD and Leprosy Control, 22 437 leprosy cases detected during 1989－ 1998 in China were analyzed in terms of leprosy disability. Results The proportion of disabilities in newly detected leprosy cases in 1989 in whole country was 46.49％ and decreased to 32.31％ in 1998, and the proportions of cases with grade II disabilities were 25.55％ (1989) and 20.23％ (1998). There were 18 provinces where disability rate was more than 40％ . Out of patients with disabilities, those with grades I and II disabilities and with deformities (loss of eyebrow, facial paralysis or saddle nose) accounted for 37.86％ , 60.64％ and 1.5％ , respectively. There were 25 provinces where grade II disabilities accounted for more than 50％ of all patients with disabilities. The disability rates in patients aged under 15 years, 15－ 65 years and over 65 years were 24％ , 39.85％ and 53.33％ . The patients with a delay in detection of 2 years had a disability rate of 29.85％ , and those with a delay of more than 2 years and 5 years had the rates of 48.82％ and 61.17％ , respectively. The disability rate was 52.9％ in patients with leprosy reactions and 46.1％ in patients with a damage of more than 3 nerves. The grade II disability rate in paucibacillary patients(28.99％ ) was significantly higher than that in multibacillary ones (22.04％ ). Conclusion Disability rate of leprosy in newly detected cases is still high although it has decreased in the recent 10 years. The rate is associated with delay in detection, leprosy reaction and leprosy type. It suggests that early detection of leprosy patients, regular treatment with multidrug therapy, and management of leprosy reactions will be the effective measures to prevent disabilities of leprosy.

Objective To explore the role of human parvovirus B19(HPV B19) infection in the onset of aplastic anemia(AA).Methods The presence of HPV B19-DNA was detected in the peripheral blood samples from 22 patients with AA(aplastic anemia) by using nested polymerase chain reaction assay,and 20 healthy blood donors as control.Results Seven of 22(31 8%) AA cases were HPV B19-DNA positive,while all the samples from the control group were negative for HPV B19-DNA (P0 05).Conclusion HPV B19 infection is closely related to the onset of AA,and may be an important viral cause for AA.

Objective To investigate the influence of a catastrophic event to the mental health of servicemen,and to observe the effect of mental intervention and drug treatment for them after experiencing a catastrophic flood attack.Methods 57 servicemen survived a catastrophic flood attack were regarded as the disaster group,and 70 others who were not involved in the flood disaster were regarded as the control group.The service men of the two groups were subjected to investigation with Chinese Military Mental Health Scale(CMMHS),and corresponding mental intervention or medication was given to those who had experienced mental disorders.Results The disaster resulted in different degrees of somatic trauma and various mental disorders including instability of emotion,mental depression,grievance,nervous tension,sensation of fear,sleep disorder and apprehensions.The influential factor scores of CMMHS,including compulsive neurosis,anxiety,depression,fear,psychosis,somatization,interpersonal sensitiveness,and total score were much higher in disaster group than that in control group,and that of norm of Chinese servicemen,while the neurasthenia factor score in disaster group showed no significant difference with that in control group and that of normal Chinese servicemen.The main influential factors to changes in mental health of the servicemen were witness of someone washed away by the flood,and participation in identifying corpses.Conclusion The mental health of servicemen who experienced a catastrophic flood disaster was harmed in various degrees,and medication or mental intervention appeared to be of value.

ObjectiveTo determine the rehabilitation awareness and needs in patients with disability due to leprosy.Methods4353 patients in 23 fields of 7 provinces were surveyed by interview and questionnaire.Results80% of patients knew general knowledge of disability prevention and rehabilitation, and believed that the self-care could prevent disability; but they seldom carried out the daily self-care practice.90% of patients lacked awareness in early manifestation of leprosy, treatment of the disease, basic cause of disability, and the way to prevent such as plantar ulcers. For needs of rehabilitation education, teaching by medical staff(86%), booklet(81%), video tape(70%) and recording(52%) had been selected.ConclusionThe awareness of rehabilitation and the practice must be combined together. Rehabilitation education should be carried out in advance systemically through different ways.