Objective To observe the clinical effect of glossopharyngeal neuralgia with Medical Ozone. Methods 60 cases with glossophaiyngeal neuralgia were divided into two groups randomly,treatment group applied for glossopharyngeal neuralgia block with Medical Ozone and medicine,control group only applied for glossopharyngeal neuralgia block and medicine. Results At 7th and 14th day after treatment,visual analogue scale was lower in treatment group compared with control group(P <0.01) ,at 14th day after treatment,the total effective rate was higher in treatment group compared with control group (P < 0.05). Conclusion The clinical cure rate of glossopharyngeal neuralgia with Medical Ozone was high and it had popularization value.