1.Structural Equation Modeling:Stressful Life Events,Coping Styles and Emotional Disorder of Junior Middle Student Students
Chinese Journal of Clinical Psychology 2001;0(03):-
Objective:To study the relationship of stressful life events,coping styles and emotional disorder.Methods:345 junior middle school students were collected as sample.They were tested with Stressful Life Event Questionnaire,Coping Style Questionnaire,SAS and SDS.Results:The coping style played an important role on the anxiety and depression;and stressful life events influenced the anxiety and depression indirectly through the coping styles.Conclusion:Anxiety and depression were determined by the emotional-focused coping style.
2.Development and Evaluation of DNA Authenticity Identification Kit for Genuine Medicinal Materials Gastrodia Elata
Qiuhe MA ; Yuhe MA ; Yue LIU ; Tao LI ; Lijun GAO ; Wei XIA ; Mingcheng LI ; Yongmei QU
Chinese Journal of Modern Applied Pharmacy 2024;41(9):1198-1203
OBJECTIVE
To develop a DNA authenticity identification kit of Gastrodia elata that combined DNA extraction technology with PCR technology, and to evaluate the performance of the kit methodologically.
METHODS
The ITS2 sequences of Gastrodia elata and its common forgeries, such as amabilis root, dahlia tuber and potato, were found by the National Center for Biotechnology Information(NCBI). DNAMAN was used for multi-sequence alignment, and NCBI-primer-blast was used to design specific primers of Gastrodia elata. Improved DNA extraction method to ensure efficient extraction of authentic Gastrodia elata and its common forgeries genomic DNA, UV spectrophotometry was used to measure the concentration and purity. The PCR reaction system was optimized, the composition and reaction conditions of the kit were determined, and the commercially available gastrodia elata samples were randomly sampled.
RESULTS
The DNA purity OD260/OD280 values of the samples extracted by the developed kit were (1.87±0.13). The minimum detection limit was 10 ng·μL−1, and the result of repeated detection was the same for 3 times. Repeated freezing and thawing for 5, 10, 15, 20 times had no effect on the detection effect, and it could be stored at −20℃ for 1 year, among 10 commercially available gastrodia elata samples tested, 7 were authentic and 3 were counterfeit.
CONCLUSION
The DNA authenticity test kit is highly specific, sensitive, reproducible and stable, and the test results are accurate, it is suitable for the rapid identification of asparagus and its common forgeries.
3.Establishment and evaluation of a rapid PCR-colloidal gold test strip method for the detection of Fritillaria ussuriensis
Yu-he MA ; Cong-hui SHANG ; Qiu-he MA ; Tao LI ; Yue LIU ; Bei-zhen PAN ; Li-jun GAO ; Ming-cheng LI ; Wei XIA ; Yong-mei QU
Acta Pharmaceutica Sinica 2024;59(6):1773-1778
This study design of specific identification primers for the ITS2 sequence of