1.Curative effect of ALA-PDT combined with Quxietang douching in the treatment of cervical HPV infection
Chinese Journal of Primary Medicine and Pharmacy 2017;24(2):209-212
Objective To investigate the efficacy and safety of ALA-PDT combined with Quxietang douc-hing in the treatment of cervical HPV infection.Methods 208 female cervical PCR-HPV positive patients were randomly divided into treatment group and control group,each group had 104 cases.The control group received 10%(ALA)in cervical for 3 hours,then red light for 30 minutes once a week,a total of 3 times.Treatment group was given 10% (ALA)in cervical for 3 hours,then red light for 30 minutes once a week,a total of 3 times,and douching vagina with Quxietang,one day at a time,stay 3 weeks.After treatment for 3 months,every month,a cervical PCR-HPV check was conducted,summarized virus removal efficiency.Results The virus removal efficiency of treatment group was 89.42%,that of the control group was 73.08%,the difference was statistically significant (χ2 =7.471,P<0.05 ).The patients in the treatment group had light local mild erythema,swelling,burning pain,without treatment subside.Conclusion ALA -PDT combined with Quxietang douching method is efficient,with mild adverse reactions,the treatment group was better than the control group in removing cervical HPV infection and effectively eliminating the hidden trouble of cervical HPV.
2.Clinical Study of Chinese Medicine by Oral and Topical Application in Treating Elderly Herpes Zoster
Chinese Journal of Information on Traditional Chinese Medicine 2006;0(06):-
Objective To observe the therapeutic effect of Chinese medicine in treating elderly herpes zoster, and study the clinical evaluation of the treatment with Chinese medicine from evidence-based medicine (EBM) view. Methods All the 156 elderly patients diagnosed with herpes zoster were randomly divided into treatment group (78) and control group (78). The former group was administered with Chinese medicine by oral administration and topical application, while the latter took aciclovir alone. All the case were treated for 10 days. Results The overall response rates of Chinese medicine and aciclovir in treatment herpes zoster were respectively 98.70% and 84.62% (P
3.Emergency Treatment Experiences of Acute & Chronic Renal Failure with Hyperpotassemia
Guoan HUANG ; Weihong WANG ; Guang LIN
Journal of Chinese Physician 2002;0(S1):-
Objective To analyze the clinical conditions and emergency treatment experiences of acute and chronic renal failure with hyperpotassemia. Methods Retrospective analyzed 542 clinical cases of supervening hyperpotassemia, which were treated by hemodialysis on 186 patients of renal failure with hyperpotassemia for 5 years. Results 540 cases clinical symptom was improved, serum potassium concentration was down to normal range and hyperpotassemia disappeared in electro cardiogram. 2 cases died after salvage. Rate of success was 99.6%. Conclusion Hemodialysis therapy is the most effective treatment to hyperpotassemia. Use bicarbonate with kalium pH indicator of 3.0~3.5mmol/L in dialysis is useful to lower hyperpotassemia, and safe also. In the early stage of hemodialysis, pure ultrafiltration dehydration treatment is not suitable. There is still danger of heartbeat stop deal to high kalium in the early stage of dialysis; emergency treatments should be standby all the time.
4.Recent Development in High-speed Countercurrent Chromatography
Deshun DAI ; Yiming WANG ; Guoan LUO
Chinese Journal of Analytical Chemistry 2001;29(5):586-591
This paper reviews recent development and applications of high-speed countercurrent chromatography (HSCCC) in the separations on the analytical scale,the semi-preparative and preparative scale of natural products,antibiotics,proteins,inorganic compounds etc.Several series of two-phase solvent systems to facilitate the systematic search suitable for HSCCC are introduced.And the new countercurrent chromatography (CCC)technologies such as HSCCC coupled with MS,pH-zone-refining countercurrent chromatography,and ion-pairing Countercurrent Chromatography are explained and prospected in term of applications.
5.In-rive study of rotational characteristic of human lumbar segments
Qun XIA ; Shaobai WANG ; Guoan LI
Chinese Journal of Orthopaedics 2010;30(4):325-329
Objective To measure the vertebral rotational centers in sagittal and transverse plane. These data may be quite valuable for the design of artificial disc. Methods Ten healthy volunteers of 40-60 years old were recruited under IRB approval and informed consent. Three-dimension lumbar spine models of L_2, L_3 and L_4 were reconstructed from MR scans. Spine motions were then reproduced using combined dual fluoroscopic imaging system (DFIS) and MR scan technique during flexion-extension and left-right twisting of the body. Based on the geometrical features of the vertebrae, ranges of motion (ROM) of 3 representative lo-cations were measured from anterior to posterior, the vertebral body center, the spinal cord canal center and the spinous process tip. Rotational centers of the vertebral segments were then located by calculating the point of zero ROM. Results Motion patterns: The ROMs of L_2 with respect to L_3 (L_(2,3)) and L_(3,4) increased pro-portionally from anterior to posterior locations. During flexion-extension motion, the vertebral body center moved within a range of 0.6 mm, while the spinous process tip moved within 7.5 mm in the sagittal plane. During left-right twisting, the vertebral body center moved within 1.0 mm, while the tip moved within 1.6 mm in the transverse plane. No statistical differences were found in the ROMs between L_(2,3) and L_(3,4). Rotational centers: the rotational centers of flexion-extension in sagittal plane for both L_(2,3) and L_(3,4) segments were located at posterior one-third of the vertebral body. The rotational centers of both L_(2,3) and L_(3,4) segments were located approximately 30 mm anterior to the front edge of the vertebral body. Conclusion The anterior portion of the vertebrae was found to have smaller ROM than the posterior portion. The vertebra rotates with the center of rotation located at approximately the posterior one-third of the vertebral body in sagittal plane. However, the vertebra rotates in transverse plane with respect to a point about 30 mm in front of the vertebra. The data indicated that the different portions of the vertebra have distinct motion characters during different motions.
6.Development of a Surgical Navigation System with Beam Split and Fusion of the Visible and Near-Infrared Fluorescence.
Xiaofeng YANG ; Wei WU ; Guoan WANG
Journal of Biomedical Engineering 2015;32(2):455-459
This paper presents a surgical optical navigation system with non-invasive, real-time, and positioning characteristics for open surgical procedure. The design was based on the principle of near-infrared fluorescence molecular imaging. The in vivo fluorescence excitation technology, multi-channel spectral camera technology and image fusion software technology were used. Visible and near-infrared light ring LED excitation source, multi-channel band pass filters, spectral camera 2 CCD optical sensor technology and computer systems were integrated, and, as a result, a new surgical optical navigation system was successfully developed. When the near-infrared fluorescence was injected, the system could display anatomical images of the tissue surface and near-infrared fluorescent functional images of surgical field simultaneously. The system can identify the lymphatic vessels, lymph node, tumor edge which doctor cannot find out with naked eye intra-operatively. Our research will guide effectively the surgeon to remove the tumor tissue to improve significantly the success rate of surgery. The technologies have obtained a national patent, with patent No. ZI. 2011 1 0292374. 1.
Computer Systems
;
Fluorescence
;
Humans
;
Infrared Rays
;
Lymph Nodes
;
Neoplasms
;
Optical Imaging
;
Software
;
Surgery, Computer-Assisted
7.Effect of neuregulin-1βcombined with captopril on myocardial cell apop-tosis and related gene expression in rats with chronic heart failure
Xuehui WANG ; Huibing LIU ; Guoan ZHAO
Chinese Journal of Pathophysiology 2017;33(3):417-422
AIM:To study the effect of neuregulin-1 ( NRG-1β) or captopril alone and their combination on myocardial cell apoptosis and related gene expression in rats with chronic heart failure .METHODS:Young male Sprague-Dawley rats were randomly divided into sham-operated group, heart failure group, NRG-1βgroup, captopril group and combined treatment group .The volume overloaded heart failure model in the rats was established by aortocaval fistula .The cardiac function was evaluated by determining the changes of hemodynamics and plasma BNP level .The apoptotic index ( AI) of myocardial cells was assayed by TUNEL .The protein levels of p-Akt, Bax and Bcl-2 in left ventricular tissue were detected by Western blot .RESULTS: The LVEDP, AI and the expression of Bax were significantly lower in NRG-1βgroup, captopril group and combined treatment group than those in heart failure group ( P<0.05 ) , while the values of ±dp/dtmax and the protein levels of p-Akt and Bcl-2 were significantly higher in NRG-1βgroup, captopril group and com-bined treatment group than those in heart failure group (P<0.05).The effect of combined treatment group was more signifi-cant ( P<0.05) than that in NRG-1βgroup and captopril group .CONCLUSION: Neuregulin-1βor captopril alone and the combination of them effectively improve the cardiac function and inhibit myocardial cell apoptosis in chronic heart failure rats.The therapeutic effect of combination of NRG-1βand captopril is better than that of NRG-1βor captopril alone .
8.The relationship between telomerase activity and cell apoptosis in gastric mucous
Guoan WANG ; Haifeng LIU ; Dianchun FANG
Medical Journal of Chinese People's Liberation Army 2001;0(09):-
Objective To study the expression of hTERT protein in precancerous lesions of human stomach, and to analyze the relationship between telomerase activity and cell apoptosis, in order to elucidate the effects of telomerase activity and cell apoptosis in the pathogenesis of gastric carcinoma. Methods Sp method was employed to detect the hTERT expression and the apoptosis was determined in situ by TUNEL staining in 64 patients with chronic gastritis and gastric carcinoma. Among the 64 patients, 16 suffered from chronic gastritis with gastratrophia, 15 with intestinal metaplasia, 14 with allotypic proliferation, and the 19 remainders suffered from gastric carcinoma. Results (1) The respective positive frequency of hTERT protein expression in chronic gastritis, intestinal metaplasia, gastric allotypic proliferation and human gastric carcinoma were 25.0%, 46.7%, 64.3% and 78.9%. The positive frequency of hTERT protein expression in human gastric carcinoma was significantly higher than that in gastric allotypic proliferation, intestinal metaplasia and chronic atrophic gastritis (P
9.Relationship between hTERT expression in human gastric carcinoma and its precancerous lesions and Helicobacter pylori infection
Guoan WANG ; Haifeng LIU ; Dianchun FANG
Journal of Third Military Medical University 2003;0(19):-
Objective To study the expression of hTERT in human gastric carcinoma and its precancerous lesions and to explore the relationship between hTERT expression and Helicobacter pylori (Hp) infection. Methods hTERT expression was detected by immunohistochemical method. Hp was detected by rapid urease test and Warthin-Starry silver staining. Results The expression level of hTERT in human gastric carcinoma (78.95%) was significantly higher than that in gastric dysplasia (64.29%), in intestinal metaplasia (46.67%), and in atrophic gastritis (25.00%, P
10.Mastoscopic treatment of benign mammary gland tumors: Report of 68 cases
Guoan XIANG ; Kaiyun CHEN ; Hanning WANG
Chinese Journal of Minimally Invasive Surgery 2001;0(04):-
Objective To explore the feasibility of mastoscopic resection of benign mammary gland tumors. Methods A total of 68 cases were diagnosed as having benign mammary gland tumors(42 cases of fibroma and 26 cases of cystic adenoma) by using molybdenum target X-ray examinations.The tumor was located at lower outer quadrant in 26 cases,upper inner quadrant in 16 cases,lower inner quadrant in 14 cases,and upper outer quadrant in 12 cases,respectively.The operation was conducted via a transaxillary approach.A specially-designed flap dissector was utilized to establish a tunnel underneath the breast skin,where the air was inflated to build a surgical space.Then the tumor was resected by using an electrotome or harmonic scalpel. Frozen-section examination was routinely carried out during operation. Results The operation was successfully completed in all the 68 cases,without intraoperative complications.The operation time was 28~45 min(mean,35 min).The drainage tube was indwelled for 1~2 d.Subcutaneous effusion occurred in 1 case.Postoperative recovery was uneventful without skin necrosis or other complications.The length of postoperative hospital stay was 2~5 d(3.0?1.5 d).Follow-up checkups in the 68 cases for 3~36 months(mean,12 months) revealed good cosmetic outcomes. Conclusions Transaxillary mastoscopic resection of benign mammary gland tumors is safe and feasible,with good cosmetic results.