Child ; Female ; Humans ; Polyendocrinopathies, Autoimmune ; classification ; diagnosis ; therapy ; Treatment Outcome

Objective:To explore the clinical characteristics of patients with pituitary thyrotropin-secreting adenoma and evaluate the effect of preoperative short-acting octreotide treatment on hyperthyroidism.Methods:A retrospective analysis was performed in 40 patients with pituitary thyrotropin adenoma diagnosed in Beijing Tiantan Hospital from January 2008 to January 2018. The general data, laboratory examinations and imaging findings were reviewed and analyzed. The clinical effect of preoperative octreotide on hyperthyroidism was evaluated.Results:The age of onset year of the 40 patients (male: female = 24∶16) was (30.5±5.1) years. Among them, 35 patients (87.5%) were with macroadenoma. The most common symptoms were thyroid hypermetabolism syndrome, followed by headache, dizziness, visual field damage and hypogonadism. The thyroid function of 30 patients (75%) recovered to normal within 3-5 days after the octreotide treatment. The total effective rate of the octreotide was 90.0%. The level of free thyroxine (FT 4) before treatment in patients with more than 10 times of effective cumulative dose was significantly higher than that in patients with less than 10 doses. Conclusions:Thyroid hypermetabolism syndrome and pituitary occupying effect are the most common clinical manifestations of thyrotropin-secreting adenoma. Preoperative octreotide treatment can effectively control hyperthyroidism. The level of FT 4 is a crucial factor affecting the efficacy of octreotide.

Spermiogenesis is a complex process of differentiation and morphologic alteration, in which sperm acrosome formation is an important stage. Acrosome is an essential component of the sperm head, which develops in four distinct phases: Golgi, cap, acro- somal, and maturation, each supported by precise and orderly regulation of various genes. The regulatory genes which act on Golgi ap- paratus include GOPC, Hrb, SPATA16, PICK1, and CK2α', those involved in the cap phase are Fads2, syntaxin 2, Kdm3a, and UBR7, and participating in acrosomal and maturation phases are KIFC1, Rnf19a, and DPY19L2. The abnormalities of these genes may affect male fertility by influencing the connection of the nuclear dense lamina and acroplaxome with the nuclear membrane and then the fusion and transportation of vesicles. This review focuses on the genes involved in different phases of acrosome formation.
Acrosome ; physiology ; Animals ; Golgi Apparatus ; Male ; Mice ; Sperm Head ; physiology ; Spermatids ; growth & development ; Spermatogenesis ; genetics ; Spermatozoa ; growth & development

The epitope-G1 gene of Bovine ephemeral fever virus (BEFV) glycoprotein was synthesised by PCR and cloned into expression vector pPIC9K to construct recombinant plasmid pPIC9K-G1. Then the pPIC9K-G1 was linearized and transformed into Pichia pastoris GS 115. The recombinant P. pastoris strains were selected by a G418 transformation screen and confirmed by PCR. After being induced with methanol, an expressed protein with 26 kDa molecular weight was obtained, which was much bigger than the predicted size (15.54 kDa). Deglycosylation analysis indicated the recombinant G1 was glycosylated. Western blot and ELISA tests, as well as rabbit immunization and specificity experiments indicated that the target protein had both higher reaction activity and higher immunocompetence and specificity. The recombinant G1 protein could be used as a coating antigen to develop an ELISA kit for bovine ephemeral fever diagnosis.

This paper introduces the application of H7279intelligent display chip to the selection and display of different kinds of analog electrocardiogram output by the analog electrocardiogram generator controlled by a single chip micyoco(SCM).

Objective To evaluate the drill method to prepare for healthcare-associated infection (HAI)outbreak, and strengthen the control of HAI outbreak.Methods According to two cases of surgical site infection (SSI)re-ported by a neurosurgical department on January 16,2014,HAI outbreak emergency drill was started,SSI occurred from January 2012 to January 2014 were analyzed,specimens of air,object surface and hands of health care workers (HCWs)in the SSI-related operating room and neurosurgical intensive care unit(NSICU)were taken and analyzed. Results In July 2013,3 cases of SSI occurred in neurosurgical department (1 case was suspected of SSI,and didn’t per-form cerebrospinal fluid culture),SSI didn’t exceed 2 cases in the other months,and SSI outbreak couldn’t be confirmed. 16 specimens of air,object surface and hands of HCWs in operating room were taken,microbial detection results were all qualified.13 specimens of air,object surface and hands of HCWs in NSICU were taken,qualified rate was 61.54%;and mould was detected from 2 air specimens.24 disposable objects of neurosurgical department were performed bacterial cul-ture,3 were positive,and all were qualified after repeated detection.Conclusion HAI outbreak drill is helpful for HAI management professional personnel to grasp the method of HAI outbreak investigation method,improve the correct hand washing of surgeons and nurses,strengthen the standard operating procedure,and ensure the safety of pa-tients.

Objective To evaluate the effect of capsaicin on cardiac dysfunction in diabetic rats u-sing an in vitro experiment. Methods Healthy male Sprague-Dawley rats, weighing 240-260 g, in which type 1 diabetes mellitus was induced by intraperitoneal streptozotocin 50 mg∕kg, were studied. Eighteen di-abetic rats were selected at 8 weeks after successful establishment of the model and divided into 3 groups (n=6 each)using a random number table: diabetes mellitus group(DM group), capsaicin group(CAP group)and capsaicin plus capsazepine group(CPZ group). Another 8 rats with normal blood glucose served as control group(C group). Rat hearts were quickly removed under deep anesthesia and retrogradely perfused with an oxygen-saturated K-H solution(at 37℃)using a Langendorff apparatus. Cardiac function was maintained stable for 10 min. The hearts were continuously perfused with K-H solution for 30 min in C and DM groups. The hearts were perfused with K-H solution for 20 min, and capsaicin(1.4×10-9g∕L)was then infused for 10 min via the branch of aortic cannula using micro pump in CAP group. The hearts were perfused with K-H solution for 10 min, and capsaicin receptor-transient receptor potential vanilloid type 1 antagonist capsazepine(1.4×10-7g∕L)was then infused for 10 min followed by infusion of capsaicin(1.4× 10-9g∕L)at 0.5 ml∕min for 10 min via the branch of aortic cannula using a micro-pump in CPZ group. Left ventricular systolic pressure(LVSP), left ventricular developed pressure(LVDP), left ventricular end-di-astolic pressure(LVEDP), heart rate, the maximum rate of increase or decrease in left ventricular pres-sure(±dp∕dtmax)were recorded at 10, 20 and 30 min of continuous infusion(T1-3). Results There was no significant difference in LVEDP and ± dp∕dtmaxat each time point among the four groups(P>0.05). Compared with C group, LVSP and LVDP were significantly decreased at T1-3in DM and CPZ groups and at T1-2in CAP group, and heart rate was significantly decreased at T1-3in DM, CPZ and CAP groups(P<0.05). Compared with DM group, LVSP and LVDP were significantly increased at T3in CAP group(P<0.05), and no significant change was found in LVSP or LVDP in CPZ group(P>0.05). LVSP and LVDP were significantly lower at T3in CPZ group than in CAP group(P<0.05). Conclusion Capsaicin can mitigate cardiac dysfunction in diabetic rats, and the mechanism is related to activating transient receptor potential vanilloid type 1.

Lactoferrin (LF) is an 80 kD iron-binding glycoprotein that is most highly produced in human and bovine milk and is also widely distributed in mammals. Researches of more than 70 years on lactoferrin prove that the molecule, as a nutritional molecule for mammals, possesses several physiological roles, including maintaining iron absorption, anti-microbial activity and immune modulation. Recent studies have demonstrated that LF can protect against cancer in experimental animals and has anticarcinogenic activity in many human tumors. As a natural nutrition, the "old" gene-lactoferrin has attracted attention from medical community for its "new"anticarcinogenic role.
Animals ; Antineoplastic Agents ; pharmacology ; Genes, Tumor Suppressor ; Humans ; Lactoferrin ; pharmacology ; physiology ; Milk ; chemistry ; Milk, Human ; chemistry

The IS-PCR and Rep-PCR were used to analyze 19 strains of Xanthomonas oryzae pv.oryzae from China,Japan and Philippines.Four specific primers,especially,IS1113 and ERIC could distinguish the strains from different countries.Using UPGMA analysis,most strains were in group 2 and 3.No relationship were observed between UPGMA groups and pathotypes.

Objective To explore the relationship between IL-1?-511C/T and IL-1?+3953C/T site polymorphisms and the susceptibility of pediatric epilepsy.Methods Under the case-control study,IL-1?-511C/T and IL-1?+3953C/T site polymorphisms in 117 patients with pediatric epilepsy and 95 healthy individuals controls(healthy control group) were analyzed with polymerase chain reaction restriction and fragment length polymorphism(PCR-RFLP),the relationship between IL-1?-511C/T,IL-1?+3953 C/T site polymorphisms and the risk of pediatric epilepsy were analyzed.SAS 8.0 software was used to analyze the data.Results Multiple variate logistic regression analysis revealed that compared with healthy control group,there was no relationship between the IL-1?-511C/T site polymorphisms and the susceptibility of pediatric epilepsy individuals,carrying at least one +3953T variant allele(CT and TT genotypes) had a significantly increased risk for pediatric epilepsy(adjusted OR=2.46,95%CI 1.03-5.87),compared with the wild-type genotype(+3953CC).Furthermore,individuals with epilepsy or febrile seizures family history had a significantly higher risk(adjusted OR=4.12,95%CI 1.28-29.34),compared with those with both CC genotypes.Conclusions These findings support the hypothesis that IL-1?-511C/T site polymorphisms have no relationship with epilepsy,but the IL-1?+3953C/T polymorphism may contribute to the risk of developing pediatric epilepsy.