BACKGROUND: Previous research reveals that, the morphous, structure, physicochemical property and mechanical characteristics of matrix in buffalo cornu all contribute to the biomaterials in the field of orthopaedics.OBJECTIVE: To investigate the correlation between the duration of matrix in buffalo cornu soaked in 30% hydrogen peroxide and the antigenicity, the biomechanical intensity of matrix in buffalo cornu treated by deproteinization.DESIGN, TIME AND SETTING: Measurement experiments were performed in the Mechanics Laboratory, Kunming University of Science and Technology (Kunming, Yunnan, China) from February to March in 2000.MATERIALS: Buffalo was provided by Luxi Abattoir of Yunnan Province (China), and then processed into test pieces at a nominal thickness.METHODS: The metric test pieces were rinsed with distilled water and soaked by chloroform-methyl alcohol at a ratio of 1:1 for 48 hours, then in 30% hydrogen peroxide for 24 and 48 hours, which served as the experimental group. While those remaining untreated were taken as controls.MAIN OUTCOME MEASURES: The modulus of elasticity and compressive strength were measured by electric measurement method and direct pressurization method, respectively.RESULTS: The modulus of elasticity between the experimental group and the control group had no significant difference (P>0.05). The compressive strength in the control group was significantly higher than that in the experimental group (P<0.01), and there were no significant differences between the experimental group treated for 24 hours and the experimental group treated for 48 hours (P>0.05).CONCLUSION: Subsequent to soaking in 30% hydrogen peroxide for 48 hours, the matrix in buffalo cornu maintains better biomechanicai intensity.

Asparaginase ; adverse effects ; Child ; Child, Preschool ; Desensitization, Immunologic ; Drug Hypersensitivity ; therapy ; Female ; Humans ; Male ; Precursor Cell Lymphoblastic Leukemia-Lymphoma ; drug therapy

Objective:To improve diagnosis and therapy of pseudoaneurysm by retrospectively discussing and analyzing of patients with pseudoaneurysm.Methods:The curative effects in 30 patients with pseudoaneurysm from 2003 to 2006 treated in our department were retrospectively addictive drugs and iatrogenic false.Femoral artery were constantly injuryed position.30 clinical cases were treated by surgical operations.The surgical treatment methods included pseudoaneurysm resection and arterial ligation in 9 cases,arterial suture in 3 cases,pseudoaneurysm resection and arterial suture in 15 cases,pseudoaneurysm resection and artificial vascular or autogenous saphenous vein bypass grafting in 3 cases.Conclusion:Pseudoaneurysm could be successfully cured after accurate diagnosis and proper surgical therapy.

Mutual promotion, mutual assistance and mutual inhibition are commonly for the compatibility of Chinese herbs, but they are also existed among the acupoints. In the paper, the relevant literature on acupoint, compatibility was collected and the mutual promotion, mutual assistance and mutual inhibition relationships of acupoints were described. The laws of the above mentioned combinations, the theoretic evidences and modern researches were summarized briefly. The mutual promotion and mutual assistance refer to the coordination of acupoints, presenting the relationship of the primary and the secondary about the mutual assistance. Hereby, the acupoints could be divided into the simple assistant acupoints and the acupoints from the affected meridians. The mutual inhibition is used to describe the antagonism effect in acupoint combination. There are more researches on the mutual promotion of acupoint combination but less ones on the mutual inhibition and its mechanism is just hypothetic. It is very important to have the deep study on those compatibility relationships for the improvement of acupuncture efficacy and to elaborate the effect mechanism.
Acupuncture Points ; Acupuncture Therapy ; history ; China ; History, Ancient ; Humans ; Medicine in Literature ; Meridians

AIM　To build a model of two series of PPARγ agonists thiazolidinedione and aryketo-acid derivatives using 3D-QSAR method, and to reveal the structural features affecting the binding activity to PPARγ, which relates to antihyperglycemic and antihyperlipidemic activity and has a potential application to the treatment of type II diabetes. METHODS and RESULTS　48 agonists with selective activity for PPARγ were analyzed using CoMFA. Based upon the active conformation of rosiglitazone (BRL) extracted from its complex with PPARγ all agonists were aligned. The model from CoMFA showed a high ability to explain and predict the activity of PPARγ agonists with cross-validation correlation coefficient R2=0.656, that of non-cross-validataion R2=0.982, F10，37=201.1, and SE=0.115. CONCLUSION　The CoMFA contour map indicates that the steric fields mainly contribute to the binding effect, and especially a bulky group in the arylketo-acid series favors in the increase of affinity for PPARγ, as compared to the thiazolidinedione.

Objective To study the association among the color Doppler flow pattern, vascular distribution and the lymph nodes metastasis in breast carcinomas. Methods Sixty-eight patients with infiltrating mammary gland duct carcinoma were divided into the no-lymph-nodes-metastasis group (group I , 37 cases) and the lymph-nodes-metastasis group (group Ⅱ , 31 cases), group Ⅱ were subdivided into group Ⅱa (19 cases): lateral lymph nodes positive of pectoralis minor and group Ⅱ b (12 cases): rear of pectoralis minor and subclavian lymph node positive. Blood flow parameters [peak systoltc velocity (PSV),resistent index ( RI)] and vascular Alder grades were observed and measured with color Doppler ultrasound.The results were compared with the incidence of lymph node metastasis. Results PSV and RI were not significantly different between group Ⅰ and group Ⅱ (t = -0.19,-0.63,P＞ 0.05),and also between group Ⅱ a and group Ⅱ b (t =-1.12,-013,P ＞0.05). By using vascular Alder grades,there were no significant differences between group Ⅰ and group Ⅱ (x2= 0.46, P＞ 0.05 ), but the significant differences were observed between group Ⅱ a [10.5%(2/19) ,63.2%( 12/19) ,26.3%(5/19) in Alder Ⅰ , Ⅱ , Ⅲ grade] and group Ⅱ b [8.3%( 1/12), 16.7%(2/12),75.0%(9/12)in Alder Ⅰ , Ⅱ ,Ⅲ grade](x2 = 7.41,P＜0.05). Conclusion The pattern of blood flow has no correlation with lymph nodes metastasis, vascular Alder grades of carcinoma shows some relevance with the lymph nodes metastasis in the patients with infiltrating mammary gland duct carcinoma.

Objective To explore the expression of drug transporter organic anion transporting polypeptide2(Oatp2) and P-glycoprotein(P-gp) in brains of chronic epileptic rats induced by lithium-pilocarpine.Methods Chronic epilepsy modles were elicited after status epilepticus(SE) induced by lithium-pilocarpine in adult Wistar rats.The expressions of Oatp2 and P-gp were detected by immuno-histochemistry(SABC) method.Results Positive expression of Oatp2 was predominantly in the plexus epithelial cells and(blood-brain) barrier endothelial cells.Weak staining for Oatp2 was also detected in the neurocyte.In contrast,apparent expression of(P-gp) was seen in brain capillary and neurocyte only.Compared with normal control rats,expression of Oatp2 on the plexus epithelial cells and brain capillary in chronic epileptic rats was significantly decreased(P

Objective To investigate the expression of VCAM-1 and E-selectin in vessel walls of the patients with thromboangiitis obliterans(TAO).Methods The vessel tissues were taken from 18 cases of amputation operation due to TAO and 16 cases of amputation operation due to non-vascular diseases.The expression of VCAM-1 and E-selectin were assessed by the immunohistochemical method.Results The positive expression rate of VCAM-1 in vessel wall of the patients with TAO was 77.8%(14/18),which of E-selectin was 72.2%(13/18);both of them were obviously higher than 6.3%(1/16) and 6.3%(1/16) in the control group.The positive rate of VCAM-1 and E-selectin had no correlation with the age,smoking amount,smoking time and drug use (P>0.05).Conclusion The expression of VCAM-1 and E-selectin have close correlation with the occurrence and development of TAO.Detecting the expression of VCAM-1 and E-selectin can serve as a diagnostic indicator of TAO.

OBJECTIVE:To determine the content of ?9-tetrahydrocannabinol (THC) in hemp by GC-MS. METHODS: HP-5 quartz capillary column was used. The temperature programming was as follows: the initial temperature was kept at 200 ℃ for 1 min, then raised to 270 ℃ at the rate of 3 ℃?min-1 and kept for 10 min; the injector temperature was 280 ℃and the detector temperature was 290 ℃. RESULTS: The good linearity between peak area and concentration was obtained over the range of 5.0 ～45.0 ?g?mL-1 (r=0.998 7). The lowest detectable limit was 1.0 ?g?mL-1(S/N=5).The average recovery rate was 81.1% (RSD=3.8%,n=9). CONCLUSION: The procedure is simple, accurate and reproducible, and suitable for the quality control of hemp preparation.

Objective To study the clinical evaluation of calf blood as a combination of aspirin and aspirin for ischemic stroke and its effect on peripheral blood BCL-2,BAX and Caspase-3,IL-6 and pathogens.Methods 128 patients with ischemic stroke were enrolled in our hospital from September 2014 to October 2016.The patients were divided into observation group and control group by throwing coin method.The control group was treated with oral aspirin tablets and some conventional medical treatment,and the observation group on the basis of this increase in calf blood to the protein injection for treatment,the hemorheology,Barthel index,the national institutes of health stroke scale(NIHSS score)and peripheral blood BCL-2,BAX and Caspase-3 protein content of two groups were recorded before and after treatment.Results After treatment,the total effective rate in the observation group(96.9％)was significantly higher than that in the control group(71.9％),the NIHSS score and hemorheological index of the observation group were significantly lower than those of the control group,Bclhel index was significantly higher than that of the control group,the content of BCL-2 protein in the peripheral blood of the observation group was significantly higher than that of the control group,the levels of BAX and Caspase-3 protein in the peripheral blood of the observation group were significantly lower than those of the control group,the difference was statistically significant(P<0.05).Conclusion The clinical effect of calf blood protein injection combined with aspirin in the treatment of ischemic stroke can not only improve the daily life ability of patients,but also can improve the hemorheology and peripheral blood BCL-2; BAX,Caspase-3 protein content.