Objective To investigate the influence of neuro-specific DNA repair gene Nbn on the development of granular cells in hippocampus of monatal mouse. Methods The hippocampus development of postnatal mouse was evaluated by comparing the morphological differences of granular cells in hippocampus between the Nbn gene knockout mice (Nbn-CNS-Del) and the controls (Nbn-CNS-Ctr). Serial sections, immunohistochemistry and stereology methods were used to quantitatively analyze the development of granular cells in hippocampus of mice on the postnatal day 7, 14 and 21 (P7d, P14d and P21d). Results In Nbn-CNS-Ctr group, the granular layer in hippocampus manifested "C" in shape, the section area of granular layer was increased, and so was the cell body of granular cells in hippocampus during P7d to P21d; the inner arm granular cells in granular layer of hippocampus emerged on P7d, and most obvious changes occurred during P7d to P14d. While in Nbn-CNS-Del group, the granular layer in "C" shape were seen to form in most of the specimens during P7d to P21d, though they were sparsely distributed. Compared with that of the mice in Nbn-CNS-Ctr group, the development of granular layer in hippocampus of the mice in Nbn-CNS-Del group lagged obviously, and the sectional area of granular cells decreased significantly (P

[Objective] The artical summarizes in diagnosis and treatment of postnatal lack of lactation academic thoughts and characteristics of drug use,and own thoughts. [Method] Summary by reading text and access to my perception ,combined with the actual clinical,summarized his main syndrome differentiation and treatment of postnatal lack of lactation thought,medication,and explain orally .[Results] FU Qingzhu according to the cause of postnatal lack of lactation divided into two types, deficiency of Qi and blood,blockage of liver. Pointed out that the error appeared in the dialectical and put forward the correct opinions;and that the disease is mainly related to the lack of blood and liver qi stagnation; the main therapy is tonifying qi and blood, smoothing liver qi, dredging collateral for promoting lactation;the corresponding therapeutic prescriptions were given and analyzed;clinical application of good results.[Conclusion] FU Qingzhu 's idea put forward the original opinion and treatment of medicine, in order to inherit traditional Chinese medicine academic, improve the curative effect of postpartum hypogalactia,and give my opinion, may be the hope of the authority.

Objective To investigate the morphological changes during the development and aging of C57/BL6 mouse hippocampus.Methods Serial sections and stereology were used to quantitatively analyze the development of C57/BL6 mouse hippocampus at different growth stages.Results Hippocampus primordium first appeared at embryonic day 12(E12d).A "C" outline could be seen in the pyramidal layer of Ammon's horn(CA),and the extra-arm's blanket of the dentate gyrus(DG)'s granular layer formed at E18d.After birth,CA developed and maturated gradually.The blanket of DG's granular layer formed at postnatal day 7(P7d).At P21d,the inner arm's thickness of DG's granular layer was equal to that of the extra-arm,and the subgranular layer was present until month 15(15M).The increase of the volume of hippocampus,CA,DG and CA's layers was slow before P7,became fast from P7d to P14d and slowed down again after P14d,but became stable after 3M.Conclusion Mouse hippocampus is formed at E12 and becomes basically mature at month 3.The volume of aging mouse hippocampus has no obvious changes.

Objective To evaluate the value of SPECT/CT for detecting the causes of lumbago in post-lumbar spinal fusion patients. Methods From January 2009 to December 2015, 53 patients (31 males, 22 females, age: (42.3±5.3) years) with lumbago after lumbar spinal fusion, without positive CT and digital radiography (DR) findings, were included in this study and were further examined by SPECT/CT imaging. The final diagnosis was made according to clinical symptoms, multimodality imaging findings, and treatment outcome. All patients were followed up for at least 6 months. Results Thirty-eight patients had positive findings with SPECT/CT imaging, including 23 patients with screw loosening, 6 patients with lumbar fusion cage aseptic inflammation and 9 patients with impingement. The other 15 patients had negative results. The diagnostic sensitivity of SPECT/CT for detecting causes of lumbago was 71.7% (38/53). The diagnostic sensitivity and positive predictive value of SPECT/CT for complications caused by internal fixators were both 100% (38/38). Conclusion SPECT/CT scanning could be a clinical valuable tool for inspecting the cause of lumbago in post-lumbar spinal fusion patients.

Objective:To explore the effect of bilingual teaching applied to the classes of histology and embryology.Methods The bilingual teaching was tentatively preformed in a part of the undergraduates in our school during their classes of histology and embryology.Results The effect of bilingual teaching was obviously superior to the traditional one.The theory examination scores and the ability of reading and using professional English were higher in the class which employed the bilingual teaching than in the control ones.Some students thought that the bilingual teaching could mobilize their activities.Conclusion The application of bilingual teaching in Histology and Embryology has a positive significance to improve the quality of teaching.

China ; Cleft Lip ; etiology ; therapy ; Cleft Palate ; etiology ; therapy ; Humans

Objective To study action mechanism of rhamnocitrin in Oxytropis Falcata on enhance the immune function. Methods Kunming mice were randomly divided into blank control group, model group, positive control group, traditional Chinese medicine high, medium, and low dose groups. All treatment groups received gavage with relevant medicine for 10 days. From the 8th day, blank control group received intraperitoneal injection with normal saline, while other groups received intraperitoneal injection with cyclophosphamide for 3 days. Through macrophages carbon clearance test, macrophage phagocytosis of chicken erythrocytes test, and immunosuppressive mice organ index detection, effects of rhamnocitrin in Oxytropis Falcata on nonspecific immune function of immunosuppressive mice were observed. Results Compared with model group and blank control group, traditional Chinese medicine high, medium, and low dose groups could improve the phagocytic activity of immunosuppressive mice, clearance index, and phagocytic coefficient (P<0.05), and increase the organ index of the thymus and spleen. Compared with the positive control group, the efficacy of traditional Chinese medicine high, medium, and low dose groups was low. Conclusion Rhamnocitrin in Oxytropis Falcata can enhance the immune function, improve the formation of spleen cells of mice serum hemolysin of chicken red blood cell immune.

Non-alcoholic fatty liver disease (NAFLD) has become a new health issue in the world due co its increasing incidence rate,and in particular,nonalcoholic steatohepatitis is progressive and has poor prognosis.Therefore,there is an argent need to search for the methods for the prevention of disease progression and treatment.Bile acid,as an important metabolite and signal molecule,can adjust the metabolism of lipids and carbohydrates and energy balance inside and outside the liver.Bile acid interacts with is receptors,such as the farnesoid X receptor and Takeda G-protein coupled receptor 5,bile acid transporter,and gut microbiota and is involved in the pathogenesis of NAFLD and nonalcoholic steatohepatitis at different levels.This article summarizes the research advances in the pathogenesis of bile acid-related NAFLD and related pharmacotherapy.

ObjectiveTo observe the chronological and spatial expressions of transforming growth factor-β receptor typeⅡ(TGF-βRⅡ)and fibronectin (Fn )in developing mouse kidney and investigate the relationship between the expressions and kidney development. Methods Immunohistochemistry and Western blotting were used to observe and measure the expression of TGF-βRⅡ and Fn in embryos and postnatal mouse kidneys. Results The expression of TGF-βRⅡ was noticed in all stages of glomeruli, renal tubes and collecting ducts, and increased gradually with ages; The expression of Fn was noticed too in all stages of glomeruli, renal tubes and collecting ducts, except for the comma-shaped bodies, and also increased gradually with ages. Conclusion TGF-β and Fn might play an important role in the development and maturation of mouse kidney.

Objective To study the diagnostic value of achilles tendon injury(ATJ) and the diagnostic standards for different severities of the injury by MRI.Methods Twenty-six cases of traumatic ATJ proved both clinically and surgically and had complete MRI materials were selected.The characteristic MRI appearances were summed up and retrospectively compared with that of 10 normal achilles tendons.Results The results were as follows :(1) MRI appearances of 10 normal achilles tendons : regular course,smooth margin,homogenous hypointensity on T_1WI and T_2WI;(2) MRI appearances of 17 partial ATJs:tendon thickening,decreased ratio of wide/vertical diameter,localized hyperintensity in tendon on T_1WI and T_2WI,crude and irregular fibrous bundles but at least keeping one continuous layer on sagital view;(3) MRI appearances of 9 total ATJs: tendon thickened and irregular appearance,totally discontinuous fibrous bundles,"brush" or "pestle" change and mixed hyperintensity on T_1WI and T_2WI;(4) 7 cases with bone contrusion and soft tissue injury,2 cases with ligament injury,2 cases with bone and cartilage injury and 11 cases with fluid collected around tendon.Conclusion MRI can accurately judge thenature,range,degree and complications of ATJ and is of great value for preoperative plan of the treatment program and the postoperativeanalysis of the therapeutic effect.