2.Anterior plate fixation for cervical distractive flexion injuries:a biomechanical comparison of different types of design
Jian ZHAO ; Hong-Fu WU ; Guo-Ping GUAN ; Hongguang SHI ; Fan LIU ; Jian FAN ; Youhua WANG ;
Chinese Journal of Orthopaedic Trauma 2004;0(06):-
Objective To evaluate the safety of dynamic anterior plate fixation for cervical distractive flexion injuries and compare its rigidity between different types of plate design. Methods Twelve sets of cadaveric calf spine were used in this test. All the specimens were made into distractive flexion injury models (C4-C5) ac- cording to Allen's method. After discectomy and grafting, they were randomized into three groups in which Orion, Codman, and Window instrumentations were used respectively. The stiffness of each construct was tested in flexion, extension, lateral bending and axial torsion conditions sequentially. Results Compared with an intact cervical spine, the range of motion (ROM) of an injured cervical spine increased whatever plate was applied. Orion in- strumentation presented stiffness the closest to that of the normal control, except for less torsional stiffness. Codman instrumentation provided stiffness close to that for normal and Orion groups only in lateral bending. Window's was the weakest mad not enough in all kinds of movement. Conclusions Static anterior fixation is the first choice for cervical injuries. Dynamic plate fixation may sacrifice stiffness to some extent, especially when a shifting kind of design is to be chosen.
3.Effects of adrenomedullin regulating inducible nitric oxide synthase on proliferation and apoptosis in hypoxic pulmonary artery smooth muscle cells.
Chi-guan LI ; Ai-guo DAI ; Cui-ping HUANG
Chinese Journal of Applied Physiology 2005;21(2):187-191
AIMTo investigate the effects of hypoxia on the proliferation and apoptosis of PASMC, to evaluate the role of iNOS protein expression and ADM on the hypoxic pulmonary hypertension (HPH) pathogenesis.
METHODSTo culture rat pulmonary artery smooth muscle cell (PASMC), cultured PASMC cells were grouped into: normoxic group; hypoxic group; hypoxia + L-NAME group; hypoxia+ ADM group. Proliferation of PASMC were investigated by MTT and PCNA. Apoptosis of PASMC were examined by flow-cytometry. Westen blot was used to measure protein expression of iNOS induced by hypoxia.
RESULTS(By MTT, the value of 24 h hypoxia was significantly higher than that in the normoxic group (P < 0.01), the value of the hypoxia + ADM was significantly lower than that in hypoxia group, the value of the hypoxia + L-NAME was significantly higher than those of hypoxic group and normoxic group (P < 0.01). (2) By immunohistochemistry, PCNA was poorly positive in PASMC, whereas positive after 24 h hypoxia (P < 0.01), ADM inhibited the expression of PCNA significantly (P < 0.01), whereas L-NAME increased the expression of PCNA significantly (P < 0.01). (3) By FCM, apoptosis index was not significantly different between the normoxic group, hypoxic group, hypoxia + L-NAME and hypoxia + ADM (P > 0.05). (4) By Western blot, iNOS expression was poorly positive in control group, positive after 4 h hypoxia (P < 0.01), increasing as the hypoxia environment continued (P < 0.01). L-NAME had no effect on iNOS protein, ADM promoted iNOS expression (P < 0.01).
CONCLUSION(1) Hypoxia stimulates the proliferation of PASMC, and has no obvious effects on the apoptosis of PASMC. (2) Hypoxia induces the expression of iNOS, ADM can increase expression of iNOS, ADM and INOS plays a role of protection in HPH pathogenesis.
Adrenomedullin ; pharmacology ; Animals ; Apoptosis ; drug effects ; Cell Hypoxia ; Cell Proliferation ; drug effects ; Cells, Cultured ; Male ; Muscle, Smooth, Vascular ; cytology ; drug effects ; pathology ; Nitric Oxide Synthase Type II ; metabolism ; Pulmonary Artery ; cytology ; pathology ; Rats ; Rats, Sprague-Dawley
4.Correlation of daily living activities with location and severity of traumatic brain injury.
Yun-ge ZHANG ; Chun-xiao LI ; Guo-fu GUAN ; Xing-rong CHEN ; Hui-min FANG ; Zhou WANG ; Xi-ping CHEN
Journal of Forensic Medicine 2014;30(6):434-436
OBJECTIVE:
To study the correlation of daily living activities with location and severity of trau- matic brain injury (TBI) and to provide a theoretical basis for improving the accuracy of expert opinion.
METHODS:
Five hundred and one cases of patients with TBI were selected. Detailed records included following: pre-injury situation, location and severity of injury, treatment and education. Daily living activi- ties scale (Barthel index) was applied to test the subjects' daily living activities. The relevance among location and severity of TBI and Barthel index was statistically analyzed.
RESULTS:
In mild TBI group, there was no significant difference in Barthel index among each location (P>0.05). In moderate TBI group, there were significant differences in Barthel index between subarachnoid hemorrhage and cerebral lobe injury, also between parietal, occipital lobes injury and frontal lobe injury, parietal, occipital lobes injury and temporal lobe (P<0.05), respectively, whereas no significant difference in Barthel index between frontal lobe injury and temporal lobe injury (P>0.05). In severe TBI, there were significant differences in Barthel index between every two different locations (P<0.05).
CONCLUSION
There is some correlation between the location of TBI and Barthel index, which provides an important reference value for analyzing and determining daily living activities after TBI.
Activities of Daily Living
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Adult
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Brain Injuries/rehabilitation*
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Female
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Humans
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Male
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Outcome Assessment, Health Care
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Trauma Severity Indices
5.Original plant identification of Dai nationality herb "Daibaijie".
Hai-Tao LI ; Li-Ping KANG ; Bao-Lin GUO ; Zhong-Lian ZHANG ; Yan-Hong GUAN ; Xu PANG ; Chao-Zhong PENG ; Bai-Ping MA ; Li-Xia ZHANG
China Journal of Chinese Materia Medica 2014;39(8):1525-1529
OBJECTIVETo identify the original plant of "Daibaijie", commonly used Dai herb.
METHODThe literature review, morphology and anatomy, pharmacognosy, molecular biology, chemistry were used to analysis.
RESULTDaibaijie's historical scientific name, Dregea sinensis Hemsl., was mistakenly given "Daibaijie" and D. sinensis have significant differences from the distribution, morphology and anatomy, pharmacognosy, molecular biology and chemical composition. "Daibaijie" matches with the characteristics of Marsdenia tenacissima (Roxb.) Moon in Flora of China in English.
CONCLUSIONDaibaijie's original plant is M. tenacissima (Roxb.) Moon. The description and illustration of M. tenacissima (Roxb.) Moon in Flora of China in China are wrong. The illustration of M. tenacissima in Flora of China in English is wrong too.
China ; ethnology ; Herbal Medicine ; Marsdenia ; anatomy & histology ; classification ; Medicine, Chinese Traditional ; Plant Components, Aerial ; anatomy & histology ; classification
6.Chemical constituents of Spatholobus suberectus.
Ren-Neng TANG ; Xiao-Bo QU ; Shu-Hong GUAN ; Ping-Ping XU ; Yang-Yang SHI ; De-An GUO
Chinese Journal of Natural Medicines (English Ed.) 2012;10(1):32-35
AIM:
To investigate chemical constituents of Spatholobus suberectus Dunn.
METHODS:
Isolation and purification were carried out by column chromatographic methods. Compounds were characterized based on their physical characteristics and spectra data.
RESULTS:
Seventeen compounds were isolated from ethanol extract of S. suberectus. The structures were elucidated as prestegane B (1), (2R, 3R)-buteaspermanol (2), (+)-medioresinol (3), (2R, 3R)-3,7-dihydroxyflavanone (4), benzeneethanol (5), 4, 7, 2'-trihydroxy-4'-methoxyisoflavanol (6), naringenin (7), blumenol A (8), protocatechuic acid ethyl ester (9), liquiritigenin (10), 7, 4'-dihydroxy-8-methoxy-isoflavone (11), 3, 5, 7, 3', 5'-pentahydroxyflavanone (12), protocatechuic acid (13), glycyroside (14), 8-methylretusin-7-O-β-D-glucopyranoside (15), 3, 3', 4', 5, 6, 7, 8-heptahydroxyflavan (16), and dulcisflavan (17).
CONCLUSION
All compounds are firstly isolated from the title plant and compounds 1, 3 were isolated from the Spatholobus genus for the first time.
4-Butyrolactone
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analogs & derivatives
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chemistry
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isolation & purification
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Fabaceae
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chemistry
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Lignans
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chemistry
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isolation & purification
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Molecular Structure
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Plant Extracts
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chemistry
7.Clinicopathological observation On two sensory,neuronopathy cases
Yan-Ping WEI ; Yu-Pu GUO ; Jian-Guo MA ; De-Hong LU ; Lin CHEN ; Hong-Zhi GUAN ; Hai-Tao REN ; Yan-huan ZHAO ; Li-li SHI
Chinese Journal of Neurology 2001;0(02):-
Objective To determine the clinical features,neurophysiological characteristics and cervical magnetic resonance imaging of sensory neumnopathy,and to describe the pathology of skin nerve, sural nerve and spinal dorsal columns.Methods Two patients who died from sensory neuron disease (SND)after infection of digestive tract were discussed including clinical features and ancillary tests which included neurophysiology and pathology of peripheral nerve and spinal dorsal columns.Associated documents are reviewed.Results Early ataxia,widespread sensory symptoms and global loss of deep tendon reflex were the distinctive signs of SND,which was characterized by non-length-dependent abnormalities of sensory nerve action potentials,a hallmark of ganglionopathies.The second patient showed normal cervical magnetic resonance imaging possibly because of short course of disease,while diffuse hyperintensity in the spinal posterior columns of SND was reported.Demyelination of spinal posterior columns and loss of mostly large diameter nerve fibers without regeneration clusters were the main pathological features.Conclusions The distinctive clinical features and neurophysiological characteristics of SND indicate that peripheral sensory nerve fibers are widely damaged.Pathology of spinal posterior columns confirm that central sensory pathway are impaired which allow the localization of the pathologic site to the dorsal root ganglion neurons.Cervical spinal MRI of SND are possibly normal at early phase.
8.Anti-tumor effects on human laryngeal carcinoma Hep-2 of recombinant fowlpox virus expressing chicken anemia virus Apoptin gene.
Guofang GUAN ; Ningyi JIN ; Xiao LI ; Lili SUN ; Chunshun JIN ; Wei LOU ; Ping SHI ; Yanru HAO
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2009;23(6):264-270
OBJECTIVE:
To investigate the anti-tumor effects and the mechanism of the recombinant fowlpox virus expressing Apoptin gene on human laryngeal carcinoma Hep-2.
METHOD:
Hep-2 cells cultured in vitro were infected with vFVApoptin. The anti-tumor effects on Hep-2 cells were measured through MTT staining and, the mitochondrial trans-membrane potential (delta psi m) and reactive oxygen species (ROS) were analyzed by flow cytometry. Western blot was used to detect the release of cytochrome c (Cyto c). Caspase-3/9 activities were measured by colorimetric assay.
RESULT:
vFVApoptin could restrain Hep-2 cells significantly and, had the function of down-regulating delta psi m, up-regulating ROS, promoting Cyto c release and activating Caspase-3/9.
CONCLUSION
Cyto c were released from mitochondria by the function of up-regulating ROS of vFVApoptin. Cyto c triggered Caspase-9 and, after the activation of Caspase-9, downstream apoptotic factors, such as caspase-3, were activated. Eventually, Hep-2 cells were suppressed by mitochondrial pathway apoptosis induced by vFVApoptin.
Animals
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Apoptosis
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drug effects
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Capsid Proteins
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genetics
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pharmacology
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Chicken anemia virus
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genetics
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Fowlpox virus
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genetics
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Humans
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Tumor Cells, Cultured
9.Study on spaceflight mutagenesis Platycodon grandiflorum via FTIR.
Xi-hua GUO ; Ying GUAN ; La-hu YANG ; Hong-yan ZHANG ; Jian-ping ZHOU ; Jin-shan SHI
China Journal of Chinese Materia Medica 2008;33(16):2005-2007
OBJECTIVETo establish a method for analyzing amorphous organic compound components of space Platycodon grandflorum rapidly.
METHODFTIR was applied for measuring and comparing P. grandflorum of comparison group,the ground group and the 4" generation of space group.
RESULTSeveral active components (platycodin, polysaccharide etc.) contents of space group are increased obviously.
CONCLUSIONThe major components and the structures remained inextenso,and the effictive component contents are enhanced in the space P. grandiflorum. FTIR is a fast method to analyze the changes of amorphous organic compound components of the space traditional Chinese medicines.
Mutagenesis ; physiology ; Platycodon ; chemistry ; Polysaccharides ; chemistry ; Saponins ; chemistry ; Space Flight ; Spectroscopy, Fourier Transform Infrared ; methods ; Weightlessness
10.Current progress of research on pharmacologic actions of salvianolic acid B.
Wei CAO ; Xiao-Wen GUO ; Hu-Zhan ZHENG ; De-Ping LI ; Guan-Bo JIA ; Jie WANG
Chinese journal of integrative medicine 2012;18(4):316-320
As one of the main water-soluble composites of Radix Salviae, salvianolic acid B is a phenolic acid ingredient of the Chinese drug, which is rich content in the herb and has strong pharmaceutical activity. It is used to treat cardiocerebral vascular diseases, antagonize hepatic/renal fibrosis, prevent cancer, and promote stem cell proliferation and differentiation. In the researches of its acting mechanisms, rather deepened studies have been carried out for its application on cardiocerebral vascular diseases, but that for others are rather fewer.
Benzofurans
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pharmacology
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therapeutic use
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Biomedical Research
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trends
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Disease
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Humans
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Medicine, Chinese Traditional
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trends
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Stem Cells
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drug effects
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Ventricular Remodeling
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drug effects