Nerve growth factor (NGF) can promote the outgrowth of neurites of the target ganglia. In order to further explore the relationship between this effect and the synthesis of RNA and DNA in the neurons, an autoradiography of 3~H-uridine and 3~H-thymidine was used. Superior cervical ganglia (SCG) from newborn rats were cultivated by Maximow's double coverslip method. All cultures were divided to one group of cultures a crude preparation of NGF was added to the medium and another group without NGF served as control. Before tissue culture was stopped, the. covership cultures were transferred to thelabeling-medium and incubated, and then they were fixed, and cut into serial sections and subjected to autoradiographie processes. The results show that the percentage and the level of grains of neurons labeled by 3~H-uridine in the NGF group are higher than that of control. Moreover, before the growth rate of neurites reaches a peak, the level of grains of neurons labeled by 3~H-uridine in the NGF group is obviously increased. The evidence suggests that NGF can promote the synthesis of RNA in neurons of SCG, which has a direct bearing on the quick outgrowth of neurites. In the experiments with 3~H-thymidine incorporation, that the NGF may promote the synthesis of DNA in some neurons of the third day SCG in vitro was also observed.

Objective To research the optimum hydrolysis conditions of oyster protein so as to raise the protein recovery and degree of hydrolysis.Methods Considering protein recovery,degree of hydrolysis,bitterness and clarity,the best enzyme was selected;Orthogonal test was designed to determine the best enzyme hydrolysis conditions;Based on hydrolysis and protein recovery,the effect of heat treatment,ultrasound and microwave processing and handling on enzymatic hydrolysis of oyster were investigated.Results and Conclusion Bromelain protease was suitable for the proteolysis of oyster,and the optimal conditions were:pH 6,temperature 55℃,substrate concentration 1∶3,E/S=800U/g,4 hours.In the optimal condition,the recovery of protein and degree of hydrolysis were 67.55%and 29.86% respectively in the hydrolysate.Heating,ultrasound technology and microwave treatments before hydro-lysis were harmful to enzymatic hydrolysis of oyster.

Objective To study the application values in chest bedside photograph of newborn by CR.Methods 210 CR images of newborn chest bedside photograph were analyzed retrospectively and evaluated.Class A is 201 leaves,the A rate is 95.7%;Class B is 9 leaves,the B rate is 4.3%;Class waste is 0,the waste rate is 0.Results The application value can be improved by appearing digitalization imaging and entering PACS system.Conclusion In spite of the characteristic in the faster diagnosis speed,the lower exposure of X-ray,the higher quality image and succeed rate,it can be given guides for clinic image diagnose.

Vogt-Koyanagi-Harada (VKH)syndrome is an autoimmune disease attacking against pigmented cells, resulting in blindness and usually affecting multiple organs including ears, meninges, hair and skin.Correct diagnosis and immediate treatment in the early stage is vital to visual prognosis.Currently, corticosteroids is first-line drug.In addition, VKH patients refractory to corticosteroids can choose other treatment such as immunosuppressive agents and biological agents.

Objective To investigate physical activities of preschool children by gender and to explore the effects of activity type on bone indexes.Methods During 2009 and 2010,397 preschool children of 3-5 years old were randomly selected from 4 kindergartens in Chengdu Province of China.Ultrasound bone analyzer was used to assess children's bone mass.A physical activity questionnaire was completed by parents to evaluate physical activities at leisure time.Student's t test and least square regression were used for data analysis.Results Concerning activity types,boys spent more leisure time on running and Wushu than girls did (t values were 1.94 and 2.84,respectively ; both P ＜ 0.05).However,girls spent more time on dancing (0.78 h),jumping rope (0.08 h) and manual labour (0.22 h) each day (t values were-9.50,-3.43 and-1.92,respectively; all P ＜ 0.05).The weekly total exercise time and energy consumption per unit of body weight of girls vs.boys were 7.29 vs.6.51 h and 127.57 vs.113.85 kJ (t values were 2.63 and 2.04,respectively ; both P ＜ 0.05).About per day time on sleeping and per week time on watching television,there were no significant difference between boys and girls (t =0.180,0.520;P ＞0.05).But boys spent more time on electronic game and computer than girls (t =0.760,2.510;P ＜ 0.05).The normalized correlation coefficient for bone mass and moderate physical activities or jumping was 0.184 and 0.275,respectively (both P ＜ 0.05).Conclusions Our data suggest that preschool children's bone volume may be positively correlated with moderate physical activities and jumping activities.Introducing some moderate physical activities or activity appliance,toys and playing fields as well as increasing professional training might be helpful.

[Objective]To investigate the effect of a single subcutaneous dose of an antisense oligonucleotide(ASO) on particle-induced osteolysis.[Method]The murine calvaria osteolysis model was utilized in ICR mice.Bone resorption was measured with the toluidine blue staining.Osteoclasts were detected by tartrate resistant acid phosphatase(TRAP) staining assay and were quantified by a TRAP quantification kit.[Result]Bone resorption was 0.347 ? 0.09 mm2 in animals with particle implantation,and decreased to 0.123 ? 0.05 mm2 and 0.052 ? 0.02 mm2 after ASO treatment in low and high doses,respectively.The bone resorption was reestablished in animals given an additional TNF-?.The number of osteoclasts in animal calvaria treated with ASO was reduced obviously compared with those untreated animals and the quantification results indicated that about 90% osteoclastgenesis was suprressed by the ASO.Additionally,the osteoclastgenesis was reestablished by the addition of TNF-?.[Conclusion]An antisense oligonucleotide targeting an inflammatory factor,TNF-?,has been to suppress the osteolysis induced by particle for the first time.This new finding holds a great promise.It is a therapeutic strategy for the component loosening.

Objective To study the character of transformation of H.pylori into coccoid form under oxidative stress, and the changes in the biological charasteristics after transformation. Methods The coccoid form of H.pylori was studied with bacteriological, enymological, and genetical methods to identify its differences from the original form. Animal experiment was conducted to determine its implantation capacity. Results Morphological transformation from spiral to coccoid form occurred with the prolongation of culture under oxygen stress. The metabolic activity was reduced gradually, finally maintained at a lower level. The activities of urease, alkaline phosphatase and acid phosphatase were decreased markedly. The mRNA levels of main virulence factors were declined. Both mRNA and activities of enzyme of Sod B and Kat retained high levels. A part of the coccoid forms showed reversion to helical form, and colonization in mice stomach could be found after being inoculated to mice. Conclusions The metabolism and virulence of H.pylori were all lowered under oxidative stress, but a part of the coccoid form may be viable. Sod B and Kat may play an important role in the oxygen metabolism of H.pylori.

Objective To prepare the “bacterial ghost” of E.coli with heat-induction and analyse its lysis rate and configuration. Methods Through immediately shifting the culture temperature from 37℃ to 42℃, E.coli DH5? including plasmid pMuH36 was induced to lyse, and the OD value of culture media was measured every 30 minutes during the induction. After 4 hours of induction, the bacteria samples were collected to examine the lysis rate by CFU (colony formation unit) and the configuration of lysed bacteria was observed by transmission electron microscopy (TEM). Results The OD value of DH5? (pMuH36)began to decline after 1 hour of induction, and increased slowly after 4 hours of induction. The CFU assay showed that the lysis rate was about 95%. TEM observation proved that most of the lysed bacteria were emptied, whereas the whole outmembrane structure, i.e. “bacterial ghost”, remained. Conclusions The E.coli “bacterial ghost” was efficiently prepared and identified, which might provide the basis for further development of a more effective “bacterial ghost” vaccine and adjuvant.

Objective:To determinate the substances with high molecular weight in porcine anterior pituitary and adrenal cortex extracts injection. Methods:The HPLC analysis was performed on a TSK-GEL G2000SWXL(7. 8 mm × 300 mm,5 μm) column with the mobile phase of trifluoroacetic acid-acetonitrile-water(0. 05:35:65). The flow rate was 0. 5 ml·min-1, and the detection wave-length was 214 nm. Results:The substances with high molecular weight in porcine anterior pituitary and adrenal cortex extracts injec-tion was below 1%. Conclusion:The method is simple and repeatable, which can be used for the control of the substances with high molecular weight in porcine anterior pituitary and adrenal cortex extracts injection.

Abstract?AIM: To analyze the effects of femtosecond laser assisted cataract surgery ( FLACS ) in the treatment of cataract and its effect on prognosis.?METHODS:Forty-two cases (42 eyes) of patients with cataract who were treated in the Department of Ophthalmology in our hospital between January 2012 and December 2014 were selected as the study objects. According to the order of treatment, they were divided into control group and observation group, 21 cases in each. The control group was treated with traditional phacoemulsification cataract surgery ( PCS ) . On the basis, the observation group was treated with femtosecond laser. The effective phacoemulsification time (EPT), cumulative dissipated energy (CDE), fluid flow and monitored pressure of the two groups were recorded.The rate of corneal endothelial loss and the situation of Tyndall phenomenon were statistically analyzed.The two groups were followed up for 1a.The long-term visual acuity recovery was observed.The best corrected visual acuity ( BCVA ) was recorded, and the long-term complications were statistically analyzed.?RESULTS: 1 ) The total response rate in observation group was 95% while in control group was 90% ( P>0.05);2) the surgery time of the observation group was longer than that of the control group ( P<0.05) but EPT was shorter than that of the control group.CDM and liquid flow were less than those of the control group ( P<0.05 ); 3 ) at 1d after surgery, there was no significant difference in intraocular pressure between the two groups (P>0.05); the rates of Tyndall phenomenon and corneal endothelial loss in the observation group were lower than those in the control group ( P<0.05);4) BCVA of the two groups at different time after surgery were significantly higher than that before surgery (P<0.05). However, at 1d, 3mo, 6mo and 1a after surgery, BCVA of the observation group was better than that of the control group ( P <0.05 ); 5 ) the incidence of complications in the observation group after surgery (14%) was lower than that in the control group (43%) (P<0.05).?CONCLUSION: The surgical effects of FLACS in the treatment of cataract are good.After surgery, the visual acuity of patients is improved significantly and the incidence of postoperative complications is low. However, the surgery time is long and cost is high, so it is difficult to popularize.