An information service model of subject librarians was proposed according to the semantic-linked data, changing retrieval methods of ontology technology users, and ontology.This model provides an open application in-terface by making use of SOA for the semantic-based information service, establishes the expanded semantic-linked relation between resources by constructing the driving engine and knowledge database based on linked data technolo-gy, and builds subject librarian platform, medical expert platform and subject user platform.Subject librarians can thus provide intelligent information retrieval service for medical users at semantic level under the help of medical experts.

Objective: To study the effect of different ratios of n-6 to n-3 polyunsaturated fatty acid on serum lipid in mice, on the premise that the ratio of SFA to MUFA to PUFA is 1∶1.7∶1.2 or 1∶1∶1. Methods: Ten experimental diets were made from six oils different in fatty acid composition to raise the mice for 10 w. Then the blood lipids were determined in mice fed with different experimental diets. Results and conclusion: Based on the ratio of S/M/P 1∶1.7∶1.2, which is the current dietary fatty acid intake, n-6/n-3 at 1~5∶1 will have lower serum lipid level. Based on the ratio of S/M/P 1∶1∶1 , n-6/n-3 at 1∶1 will have lower serum lipid level. Based on the ratio of n-6 /n-3 10∶1 which is the current dietary fatty acid composition abroad , S/M/P 1∶1.7∶1.2 will have lower serum lipid level.

Objective A solution of serial communication in Multi-Axis Controller which uses programmable multi-axis controller (PMAC) as down processor and PC as up processor is introduced. By the PComm32.dll provided with Delta Tau Data Systems Inc., the serial communication between PC and PMAC is realized based on .NET. Methods In order to realize the programming techniques of serial communication, a new method that managed codes invoked the unmanaged codes in Common Language Runtime environment by using Platform Invocation Services was adopted. The serial communication program was designed by using the program language C#. Besides the single axis locomotion,the debugging procession of the multi-axis joint locomotion was also recounted based on the five-DOF robot. Results The results indicated that the serial communication between PC and PMAC was completely realized. Conclusion The developed serial communication software module is of good correctness, stability and reliability, and also shows that the software is convenient and easy to use, which can accord with system requirements.

Objective To investigate the nalaxone treating first acute right heart failure.Methods52 patients with FARHF were divided into the naloxone group(30cases)and control group(20cases)at random.Patients in control group were given general comprehensively treating.Patients in naloxone group were given naloxone as well as comprehensively treating,were given intravenously 0.8 mg of first dose,if it was necessary,repeated 0.8 mg of dose,and continuously dropped for 1.2 mg was added into liquid 500 mL.After treatment of 24 hours,were compared the changes of HR、SBP、SV and heart function.ResultsIn the naloxone group,the total effective rates were 84.4%,the mortality rates were 6.6%;in control group.The total effective rates were 60%,the mortality rates were 18.3%.The difference of the effective rate of the naloxone group be treatment was statistically significant(P

Objective To investigate the clinical value of the expert type terminal of long distance electronic fetal heart rate home monitoring system in the application to self monitoring of pregnant woman at home in peripartum Methods All the pregnant women ( n =284) were divided into two groups Research group ( n = 134) contained 73 high risk gravida There were 78 high risk gravida in control group ( n =150) In the research group, self monitoring at home in 134 women was taken by the expert type terminal of long distance electronic fetal heart rate monitoring system through telephone and fetal movement counting The women were requested to auscultate and transfer fetal heart rate (FHR) to electronic FHR monitoring center in hospital Non stress test(NST) was made 1～2 times every week when fetal movement was active When women felt any abnormality of baby, she should immediately test NST and then the photograph of NST was send to FHR monitoring center by telephone Doctor would make a diagnosis and management in time according to the photograph of NST In the control group, the way of fetal monitoring was fetal movement counting and regular NST test in the outpatient clinic Results The incidence of abnormal NST was significantly higher in the research group than that in the control group (respectively 22 0% vs 13 5%, P 0 05) Conclusions The expert type terminal of long distance electronic FHR monitoring system through telephone is a new way of the FHR self monitoring at home The application of this method could ease mental press of the women, decrease significantly perinatal mortality, decrease incidence of neonatal asphyxia, and improve quality of obstetrics Whatever there are high risk factors in any pregnant women, the system should be appied to all late pregnant women

Objective:To observe characteristics of TCM syndrome types in the patient of cerebral infarction,and cerebral infarction with respiratory disorder at sleep.Methods:90 in-patients of cerebral infarction were monitored by polychannel sommocinematogram (PSG)within 72 hours of admission,which were used as diagnosis of respiratory disturbance at sleep,and all of the patients with apoplexy involving the channels and collaterals were divided into 3 types,collaterals hollow and pathogenic wind attacking the interior;Yin-deficiency of the liver and kidney,and wind-Yang stirring upper,hot phlegm and excess of Fu-organs,and wind- phlegm stirring the upper.Results:The patient had sleep structure disorder,with large change of blood pressure and lower heart variety,and decrease of degree of blood oxygen saturation.The apnea indexes were the highest for the patient with phlegm-heat hollow-organ excess,wind-phlegm stirring the upper,and baic normal for collateral hollow and pathogenic wind attacking the interior.Conclusion:There is close relation of cerebral infarction,especially in the patient of hot phlegm and excess of Fu-organ, with respiratory disorder at sleep.

This experiment has proved that bolbostemmosaponin and its constituents A and D have relatively powerful spermatocidal effects. They can kill all spermatozoa in a moment at the concentrations of 0.04%, 0.04% and 0.03% respectively. These spermatocidal agents mainly distroy the biomembrane system of sperm. Using the fixed gelatin membrane technique proved that all of them can strongly inhibit tne activity of a single sperm acrosin at the concentration of 0.05%.

The study is aimed to confirm the silencing efficiency of the vector in human hepatocellular liver carcinoma cell line (HepG2), and observe effects of AMPKgamma silencing on the AMPK stimulating activity and lipid synthesis of cordycepin (CCS), a natural product with known AMPK activating function. The downregulating efficacy of siRNAs on AMPKgamma expression was confirmed in our previous study. The double stranded shRNA Oligo was ligated to lentivirus vector and verified by sequencing. The lentiviral which can effectively inhibited protein expression levels of AMPKgamma was selected by Western blotting, and the regulation of CCS on protein expression of AMPKgamma and p-AMPK in AMPKgamma silence cells were detected by Western blotting analysis. The lipid accumulation in cells was observed by Oil-Red O stain and cells were collected for the estimation of cholesterol (TC), triglyceride (TG). The results showed that the lentiviral vector carrying a shRNA targeting the AMPKgamma gene was successfully constructed. Western blotting analysis confirmed that GR085 had the highest interfering efficiency. Treatment with CCS can significantly increase the levels of phospho-AMPK in normal cells, and the level of TC, TG was reduced, but in AMPKgamma silence cells the effects of CCS on AMPK activation and lipid synthesis were almost completely abolished without changing the expression levels of total AMPK or AMPKgamma protein. In conclusion, the AMPKgamma gene may be related to AMPK activation and intracellular lipids regulation by CCS.

Objective To assess the efficacy of radical transurethral electrovaporization for invasive bladder cancer. Methods Thirty-six patients with invasive bladder cancer from february 2008 to February 2010 were treated by transurethral electrovaporization resection of bladder tumor (TURBT). The operation procedure was based upon the principle of radical transurethral resection for bladder tumor. The tumor was resected to fatty layer outside the bladder wall. After operation Bacillus Calmette-Guerin (BCG) irrigation of bladder was given,all patients were followed up for 3 to 24 months. Results The recurrence occurred in 12 cases in 2 years,with a recurrence rate of 33. 3% (12/36). These recurrence cases were treated with TURBT again. Six cases dead in 2 years,with a mortality of 16. 7% (6/36). Conclusion TURBT is suitable for those who are older or weak,and not medically fit for radical cystectomy or those who refuse the open surgery,which can prolong the survival time and improve the quality of life.

Objective To study the chemical composition of traditional Chinese medicine Xiasangju compound extract.MethodsThe ethanol extract of different organic solvent extraction,silica gel column chromatography,chromatography separation and purification technology for the separation of compounds,the structures were elucidated by chemical and spectral data.ResultsSix fiavonoid components were isolated from the ethyl acetate extract,respectively, kaempferol( Ⅰ ),quercetin( Ⅱ ),luteolin( Ⅲ ),quercetin-3-O-β-D-glucoside (Ⅳ),luteolin-7-O-β-D-glucoside ( Ⅴ ) and rutin (Ⅵ).ConclusionThe obtained compounds were isolated from compound Xiasangju for the first time.