Objective To study the relationship between B-type natriuretic peptide(BNP) and type 2 diabetes mellitus(DM) patients with congestive heart failure(CHF).Methods 78 patients with DM were divided into pure DM group(n=37) and DM+CHF group(n=41) ,the level of plasma BNP in patients with DM(n=78) and normal control group(n=20) was.measured.Results The level of plasma BNP in grade Ⅱ ,Ⅲ～Ⅳgroup of cardiac function were significantly higher than that in pure DM group and control group(P<0.05),grade Ⅲ～Ⅳ group were significantly higher than that in grade II group(P<0.05),LVEF in Ⅱ,Ⅲ～Ⅳgroup of cardiac function were significantly lower than that in pure DM group and control group(P<0.05),grade Ⅲ～Ⅳ group were significantly lower than that in grade Ⅱ group(P<0.05) ;The level of plasma BNP was positively related to LVEF(r= -0.713,P<0.05).Conclusion BNP was an objective indicator to evaluate the severity of DM with heart failure.

Objective To study the effects of trimetazidine on cardiac function in type 2 diabetes mellitus patients with heart failure. Methods Ninty type 2 diabetes mellitus patients with heart failure were randomly divided into two groups (45 patients in control group,45 patients in treatment group). All patients in both two groups received routine therapy in order to have blood glucose under control,the treatment group was treated with trimetazidine 20mg po Tid ,treatment for 6 months. Before and after treatment ,two groups were detected left ventricular end-diastolic diameter (LVEDD), left ventricular end systolic diameter (LVESD) and left ventricular ejection fraction (LVEF), and the clinical efficacy were evaluated. Results Ater treatment, the rate of total efficiency was 68. 9% in control group, and it was 86. 7% in treatment group. The clinical efficacy of treatment group was significantly better than control group(P＜0.05) ;Ater treatment,LVEDD,LVESD were significantly reduced than those before treatment(P ＜0.05) ,LVEF was significantly higher than that before treatment (P ＜0.05);After treatment,LVEDD, LVESD in treatment group was significantly reduced than those in control group, LVEF was significantly higher than control group (P ＜ 0.05).Conclusion Trimetazidine can significantly improve the cardiac function in type 2 diabetes mellitus patients with heart failure.

Purpose:To study the expression of Ki-67 antigen in normal mucosae,benign and malignant neoplasms of colorectum and its clinical significance. Methods:33 cases of carcinomas,13 cases of benign neoplasms and 13 cases of normal mucosae of colorectum were collected. Ki-67 antigen was detected using flow cytometry.Results:The Ki-67 antigen positive fraction of normal mucosae,benign and malignant neoplasms of colorectum were 2.65%?1.51%,6.38%?2.51%,18.52%?8.53% respectivly, there being significant difference between the groups(P0.05). Conclusions:Ki-67 antigen expression is a valuable marker to distinguish the normal mucosae,benign and malignant neoplasms of colorectum. Ki-67 antigen expression has no relation with tumor size,type of histopathology,Dukes stage,location,length of illness history,age,sex of the colorectal carcinoma patients.

Endometrial blood flow is directly related to endometrial receptivity thereby affecting in vitro fertilization and embryo transfer (IVF-ET) outcomes. In recent years a growing number of studies have shown that traditional Chinese medicine (TCM) can play a role to improve endometrial blood flow and embryo transfer. Studies have confirmed that formulas based on reinforcing kidney and activating blood can promote the formation of uterine endometrial blood vessels by adjusting expressions of a variety of vessel growth factors, and regulating nitric oxide level for inhibition of vascular smooth muscle contraction of the uterus. Treatments based on differentiation of syndromes are key to the theory of TCM. Differentiation of syndromes should be combined with biomedical disease diagnosis. It is also necessary to further clarify other endometrial blood flow disorders using TCM diagnostic methods. In these cases, drugs for reinforcing kidney and activating blood are relevant, but other medicines for smoothing liver qi and nourishing blood, as well as tonifying the spleen and generating blood may also be effective. Future clinical studies should focus on the observation of different types of TCM syndromes and the research on compatibility ratio and dose-dependent relationship of Chinese medicines. Acupuncture has been used during IVF-ET for more than 10 years. Electro-acupuncture can control the expansion of the uterine arteries by inhibiting the sympathetic nerves. However, acupuncture has not been proved to regulate endometrial arteries. To sum up, TCM can be applied to improve endometrial blood flow so as to increase birth rates in IVF-ET. The combination of Chinese and Western medicines and acupuncture application will increase their combined effect, thereby obtaining greater clinical benefits.

Objective To evaluate the capability of internal transcribed spacer (ITS) region sequencing analysis to identify clinical isolates of filamentous fungi.Methods A total of 267 filamentous fungi isolates collected from clinical specimens were analyzed by ITS region sequencing analysis.Alignment of acquired sequences with known sequences in GenBank and MycoBank was conducted to identify the species of those isolates.Results ITS sequences of the 267 isolates were amplified successfully.Among these isolates, 53.9％ (144/267) were identified to species level and 44.2％ (118/267) to genus level.Only five isolates were failed to be identified at genus level as they shared >95％ homology in ITS sequence with multiple genera.Conclusion ITS region sequencing analysis is preferred for identification of clinical isolates of filamentous fungi at genus level for its high universality and great capability.When species-level identification is required, some informative DNA markers besides ITS region should be included accordingly.

Objective To study the influence of dihydromyricetin on the oxidation and non-enzymatic glycosylation in impaired glucose tolerance rats.Methods Animal model of impaired glucose tolerance rat was built by intragastrical(i.g.) injection of D-galactose generally.After 8 weeks intragastrical(i.g.) injection of dihydromyricetin(experimental group) and metformin (positive control),the levels of fasting blood glucose and tow-hour postprandial blood glucose,the insulin level and its resistance index,the levels of glycosylated hemoglobin (GHb) and advanced glycation end-products(AGEs) were detected,and the activities of superoxide dismutase(SOD) and glutathione peroxidase (GSH-Px),the content of malondiadehyde(MDA) were determined respectively.Results Compared with model group,dihydromyricetin decreased the level of two-hour postprandial blood glucose (P0.05).Conclusion Dihydromyricetin can significantly improve the state of impaired glucose tolerance rats; the mechanism may be related to its effect of improving the antioxidant activity of GSH-Px,inhibiting non-enzymatic glycation,lowering body oxidative stress and insulin resistance and promoting the use of blood glucose in peripheral organizations.

Objective To adopt the cone beam computed tomography(CBCT) to analyze the bone thickness of infrazygomatic crest with different vertical skeletal facial types in teenagers.Methods Sixty teenagers of skeletal class were collected,including each 20 cases of high angle,average angle and low angle.The CBCT scanning data of oral maxillofacial region were collected.The bone thickness at different coronary slices in buccal side of infrazygomatic crest region at 13,15,17 mm above the maxillary occlusal plane was measured.The measured data were analyzed statistically.Results The bone thickness of infrazygomatic crest was gradually thinned from down to up,and the difference was statistically significant(P＜0.05);the bone thickness(except 3 points at 13 mm from maxillary plane) at each measured point in the high,average and low angle groups showed the change trend of low angle ＞average angle＞high angle,the difference was not statistically significant(P＞0.05).Conclusion In teenagers,the bone thickness of infrazygomatic crest in skeletal class Ⅱ is gradually thinned from cranial direction and rearward,the high angle is thinnest and the low angle is thickest.

[Objective] To discuss the clinical significance of simple synovial tuberculosis treated and diagnosed by arthroscopy. [Methods] A review study of 8 patients with simple synovial tuberculosis from September 1998 to September 2004. The 8 cases with the main clinical manifestation of articular swelling were confirmed as simple synovial tuberculosis by arthroscopic examination and arthroscopic synovectomy in pathology. [Results] The 8 cases were definitely diagnosed by arthroscopy pathologically. The average follow-up period was two years and four months. For all of the 8 cases, the articular symptoms disappeared, articular function recovered well and the therapeutic effect was satisfactory. [Conclusion] Arthroscopic examination is an effective method to diagnose synovial tuberculosis at early stage. Synovectomy with arthroscopy can remove the pathological synovial membrane and is beneficial to the control of the local tubercular focus.

Objective To investigate the clinical features, diagnostic criteria and treatment of primary pulmonary lymphoma (PPL). Methods 11 patients with PPL, the diagnosis of which was made with pathological study of biopsy specimen, admitted from 1997 to 2006 to General Hospital of PLA, were retrospectively analyzed. The data collected for the present study, combined with some references, were reviewed concerning the clinical characteristics, X-ray features, pathologic features, bronchoscopic observations, diagnostic criteria as well as treatment. Results PPL was a rare disease, and was hard to have a definite diagnosis for lack of specific clinical symptoms. The most common clinical symptoms of PPL were cough, chest distress, fever etc. X-ray features included solitary or multiple nodules, ground-glass opacity, filamentous shadow and consolidation shadow. Such signs could be seen at the same time, commonly accompanied with air bronchogram. No distinct changes could be found in bronchofibroscopy. Final diagnosis of PPL could be made when a CT guided biopsy was done, followed by pathological examination with immunohistological study, or study of the tumor after surgery. Surgical operation and chemotherapy were the major effective therapeutic approach for the treatment of PPL. Prognosis was favorable in patients with low degree of malignancy of the tumor. Conclusion The clinical characteristics and X-ray image of PPL are not specific. Misdiagnosis occurs commonly. The final diagnosis of PPL depends on the pathologic examination of the PPL tissue. Appropriate CT guided biopsy is necessary for an early diagnosis. Surgery as well as ancillary chemotherapy after operation could get satisfactory results.

Objective To investigate the changes in concentration of plasma von Willebrand factor (vWF) and the effects of heme oxygenase-1 (HO-1) in rabbits with endotoxin (ET)-induced acute lung injury (ALI). Methods Twenty four male New-Zealand rabbits were randomly assigned to three groups: control group, ET group and hemin-treatment group. Saline was iv. injected into the animals of control group; in animals of ET-treatment group ALI model was reproduced with endotoxin iv. injection (700 ?g/kg); animals of hemin-treatment group received intraperitoneal injection of heme for 2 days before ET challenge (40?mol/kg, 2 times per day). Arterial blood gas assay was performed and the plasma vWF concentration was determined with ELSIA at 0h (pre-experiment), 0.5, 1, 2 and 4h time points for the animals in all three groups. When the rabbits were sacrificed, the dry/wet weight ratio of lung and HO-1 (mean density) were determined and the pathological changes in lung tissue were examined. Results Compared with control group, the partial pressure of oxygen (PaO2) and oxygenation index (PaO2/FiO2) declined, meeting the diagnostic criteria of ALI, and plasma vWF concentrations increased obviously (P