Objective To study the relationship between B-type natriuretic peptide(BNP) and type 2 diabetes mellitus(DM) patients with congestive heart failure(CHF).Methods 78 patients with DM were divided into pure DM group(n=37) and DM+CHF group(n=41) ,the level of plasma BNP in patients with DM(n=78) and normal control group(n=20) was.measured.Results The level of plasma BNP in grade Ⅱ ,Ⅲ～Ⅳgroup of cardiac function were significantly higher than that in pure DM group and control group(P<0.05),grade Ⅲ～Ⅳ group were significantly higher than that in grade II group(P<0.05),LVEF in Ⅱ,Ⅲ～Ⅳgroup of cardiac function were significantly lower than that in pure DM group and control group(P<0.05),grade Ⅲ～Ⅳ group were significantly lower than that in grade Ⅱ group(P<0.05) ;The level of plasma BNP was positively related to LVEF(r= -0.713,P<0.05).Conclusion BNP was an objective indicator to evaluate the severity of DM with heart failure.

Objective To study the effects of trimetazidine on cardiac function in type 2 diabetes mellitus patients with heart failure. Methods Ninty type 2 diabetes mellitus patients with heart failure were randomly divided into two groups (45 patients in control group,45 patients in treatment group). All patients in both two groups received routine therapy in order to have blood glucose under control,the treatment group was treated with trimetazidine 20mg po Tid ,treatment for 6 months. Before and after treatment ,two groups were detected left ventricular end-diastolic diameter (LVEDD), left ventricular end systolic diameter (LVESD) and left ventricular ejection fraction (LVEF), and the clinical efficacy were evaluated. Results Ater treatment, the rate of total efficiency was 68. 9% in control group, and it was 86. 7% in treatment group. The clinical efficacy of treatment group was significantly better than control group(P＜0.05) ;Ater treatment,LVEDD,LVESD were significantly reduced than those before treatment(P ＜0.05) ,LVEF was significantly higher than that before treatment (P ＜0.05);After treatment,LVEDD, LVESD in treatment group was significantly reduced than those in control group, LVEF was significantly higher than control group (P ＜ 0.05).Conclusion Trimetazidine can significantly improve the cardiac function in type 2 diabetes mellitus patients with heart failure.

Purpose:To study the expression of Ki-67 antigen in normal mucosae,benign and malignant neoplasms of colorectum and its clinical significance. Methods:33 cases of carcinomas,13 cases of benign neoplasms and 13 cases of normal mucosae of colorectum were collected. Ki-67 antigen was detected using flow cytometry.Results:The Ki-67 antigen positive fraction of normal mucosae,benign and malignant neoplasms of colorectum were 2.65%?1.51%,6.38%?2.51%,18.52%?8.53% respectivly, there being significant difference between the groups(P0.05). Conclusions:Ki-67 antigen expression is a valuable marker to distinguish the normal mucosae,benign and malignant neoplasms of colorectum. Ki-67 antigen expression has no relation with tumor size,type of histopathology,Dukes stage,location,length of illness history,age,sex of the colorectal carcinoma patients.

Objective To evaluate the capability of internal transcribed spacer (ITS) region sequencing analysis to identify clinical isolates of filamentous fungi.Methods A total of 267 filamentous fungi isolates collected from clinical specimens were analyzed by ITS region sequencing analysis.Alignment of acquired sequences with known sequences in GenBank and MycoBank was conducted to identify the species of those isolates.Results ITS sequences of the 267 isolates were amplified successfully.Among these isolates, 53.9％ (144/267) were identified to species level and 44.2％ (118/267) to genus level.Only five isolates were failed to be identified at genus level as they shared >95％ homology in ITS sequence with multiple genera.Conclusion ITS region sequencing analysis is preferred for identification of clinical isolates of filamentous fungi at genus level for its high universality and great capability.When species-level identification is required, some informative DNA markers besides ITS region should be included accordingly.

Objective To study the influence of dihydromyricetin on the oxidation and non-enzymatic glycosylation in impaired glucose tolerance rats.Methods Animal model of impaired glucose tolerance rat was built by intragastrical(i.g.) injection of D-galactose generally.After 8 weeks intragastrical(i.g.) injection of dihydromyricetin(experimental group) and metformin (positive control),the levels of fasting blood glucose and tow-hour postprandial blood glucose,the insulin level and its resistance index,the levels of glycosylated hemoglobin (GHb) and advanced glycation end-products(AGEs) were detected,and the activities of superoxide dismutase(SOD) and glutathione peroxidase (GSH-Px),the content of malondiadehyde(MDA) were determined respectively.Results Compared with model group,dihydromyricetin decreased the level of two-hour postprandial blood glucose (P0.05).Conclusion Dihydromyricetin can significantly improve the state of impaired glucose tolerance rats; the mechanism may be related to its effect of improving the antioxidant activity of GSH-Px,inhibiting non-enzymatic glycation,lowering body oxidative stress and insulin resistance and promoting the use of blood glucose in peripheral organizations.

[Objective] To discuss the clinical significance of simple synovial tuberculosis treated and diagnosed by arthroscopy. [Methods] A review study of 8 patients with simple synovial tuberculosis from September 1998 to September 2004. The 8 cases with the main clinical manifestation of articular swelling were confirmed as simple synovial tuberculosis by arthroscopic examination and arthroscopic synovectomy in pathology. [Results] The 8 cases were definitely diagnosed by arthroscopy pathologically. The average follow-up period was two years and four months. For all of the 8 cases, the articular symptoms disappeared, articular function recovered well and the therapeutic effect was satisfactory. [Conclusion] Arthroscopic examination is an effective method to diagnose synovial tuberculosis at early stage. Synovectomy with arthroscopy can remove the pathological synovial membrane and is beneficial to the control of the local tubercular focus.

Objective To test the reliability and validity of the Chinese Version of the Depressive Cognition Scale (DCS) among the older people.Methods The sample consisted of 570 old people,collected by a demographic questionnaire,the Chinese version of DCS,and the Center for Epidemiological Studies Depression Scale (CES-D),then all the data was analyzed by SPSS 21.0.Results The item-to-total correlation coefficients of DCS ranged from 0.65 to 0.84 (P＜0.01),and the average coefficient was 0.77.The content validity index(CVI) was 0.88.The result of exploratory factor analysis (EFA) was that one factor explained 59.72％ of the total variances,and the concurrent validity between DCS and CES-D was positive related (r=0.24-0.82,P＜0.01).Regarding CES-D as the gold standard,the sensitivity was 84.7％ and the specificity was 90.7％.The inter-item correlation coefficients of DCS ranged from 0.40 to 0.73 (P＜0.01);the Cronbach α was 0.82,and the retest reliability (intra-class correlation coefficient,ICC) was 0.92 (95％CI=0.86-0.95,P＜0.01).Conclusion DCS is proved to be a reliable and valid instrument,and it can be used to assess the depressive cognition and screen the early depressive symptoms of Chinese old adults.

Objective To test the reliability and validity of the Chinese version of the sense of coherence (SOC) scale (13-items) among the older people.Methods A random sample of 1 032 old adults was selected from community in Jinzhou,Shenyang,Dalian of Liaoning.A demographic questionnaire,the SOC-13 scale,the WHO-5 and the Center for Epidemiological Studies Depression Scale (CES-D) were used to collect the data.All the data was analyzed by SPSS 21.0 and AMOS17.0.Results The item-total correlation coefficients of SOC-13 ranged from 0.625 to 0.760 (P＜ 0.01).The result of exploratory factor analysis (EFA) was that three factors explained 74.745％ of the total variances,and the CFA confirmed the hypothesized three-factor model.SOC scale had an ideal criterion(x2/df =2.908,CFI =0.975,GFI =0.949,NFI =0.963,IFI=0.975,RMSEA=0.061).Criterion related validity test showed that the total score of SOC-13 was negatively correlated with CES-D(r=-0.55,P＜0.01),and positively correlated with WHO-5 (r=0.73,P＜ 0.01).SOC-1 3 had a Cronbach's α of 0.916,the Guttman split-half coefficient was 0.828,and the retest reliability (intra-class correlation coefficient,ICC) was 0.872 (P＜0.01).Conclusion SOC-13 is a reliable and valid instrument,which can be used to measure the lever of sense of coherence among Chinese old adults.

Objective To explore the relationship between the sleep quality and diabetic autonomic neuropathy of elder patients with type 2 diabetes mellitus.Methods A total of 90 elder patients with diabetes in Beijing Hospital was enrolled in this study.Questionnaires of Pittsburgh Sleep Quality Index (PSQI) were completed to evaluate the quality of sleep and Holter was applied to evaluate heart rate variability (HRV).Other related clinical data such as,catecholamine [epinephrine (E);norepinephrine (NE);dopamine (DA)] and diabetes complications were also collected after admission to the hospital.Patients were divided into three groups:the poor-sleeper group,the common-sleeper group and the goodsleeper group according to PSQI score.HRV and the level of catecholamine were compared among three groups.Results The level of HRV including meanNN [(743 ±58) ms vs(824 ±99)ms and (837 ± 104) ms],ASDNN [(30 ± 10) ms vs (39 ± 14) ms and (41 ± 14) ms],very low frequency(VLF) [(15.33 ± 6.10) ms2 vs (22.11 ± 7.94) ms2 and (22.66 ± 7.87) ms2],low frequency (LF) [(8.30 ± 3.95) ms2 vs (12.58±6.11)ms2 and(12.81 ±6.96)ms2] and LF/high frequency(HF) [(1.23 ±0.32) vs (1.56 ± 0.46) and (1.47 ± 0.42)] in the poor-sleeper group were lower than in both the common-sleeper group and good-sleeper group (all P ＜ 0.05).The level of catecholamine in poor-sleeper group [E:(108.91 ± 4.19) ng/L;NE:(1458.0 ± 50.35) ng/L] were lower than both the common-sleeper group [E:(120.23 ± 4.37) ng/L;NE:(1901.09 ± 131.36)ng/L] and the good-sleeper group [E:(118.23 ± 19.9) ng/L;NE:(1771.87 ± 116.73) ng/L] (all P ＜ O.05),suggesting a dysfunction in autonomic nervous system in subject in the poor-sleeper group.Conclusion Sleep quality is associated with the severity of diabetic autonomic neuropathy and might be one of clinical features for diabetic autonomic neuropathy.