Objective:To observe influence ofβreceptor blocker on heart rate variability (HRV)after military train-ing in soldiers.Methods:A total of 80 soldiers with heart rate>60 beats/min from a group army were selected,and randomly divided into metoprolol group (n=40)and control group (n=40).Metoprolol group received metoprolol succinate (12.5mg,once/d)continuously before exercise 5d,while control group received placebo of same dose, 5min dynamic electrocardiography was used to analyze HRV.Heart rate,blood pressure and HRV changes were compared between two groups before and after 5km running.Results:There were no significant difference in heart rate,blood pressure and HRV indexes between two groups before exercise;compared with control group after exer-cise,heart rate and blood pressure were lower,and there were significant reductions in standard deviation of normal to normal RR intervals calculated over the 24 h period [SDNN,(80.1±9.5)ms vs.(69.4±10.5)ms],standard deviation of normal to normal RR intervals in all 5 min segments of the entire recording [SDANN,(65.7±7.8)ms vs.(60.1±9.2)ms],SDNN index [(25.1±11.3)ms vs.(20.4±12.3)ms],root-mean square of differences be-tween successive normal to normal intervals [rMSSD,(25.2±10.7)ms vs.(19.9±11.3)ms],very low frequency [VLF,(451.1±312.9)ms2 vs.(408.3±299.8)ms2 ],low frequency [LF,(191.0±79.3)ms2 vs.(148.2±77.4) ms2]and high frequency [HF,(69.6±50.1)ms2 vs.(51.8±52.7)ms2]in metoprolol group,P<0.05 all.Con-clusion:βreceptor blocker usage before exersise can reduce heart rate variability change after military training,and is helpful to decrease occurrence of acute cardiovascular events.

A total of one hundred and sixty rats were fed with isothiocyanatelabelled candida albicans and then randomly divided into four groups: simple burn (30% TBSA full-thickness scald burn was created on the back), simple antibiotic therapy (oxacillin combind with gentamicin were injected intraperitineally), 30% full-thickness burn plus antibiotic therapy and the control group. The animals were sacrificed and examined at different time phases. The labelled candida albicans were traced in the mesentery lymph nodes, liver, spleen, kidney, lung, blood stream and urine. The labelled candida albicans could be demontrated from 30% of visceral organs at one hour and from 70% at six hours after burn plus antibiotic therapy, while the mesentery lymph node and liver were the primary organs invaded. The process of candida translocation through the intestinal wall was displayed as adhesion and then invasion. For comprative study of the methods or the early diagnosis of systemic dissemination of candida albicans, blood cultures, urine cultures and direct examination of urine under microscope were performed. The detectable rate was only 8% in blood culture and 21.8% in urine culture, however, it was 40% in direct examination of urine, the later method is therefore much more sensitive than the other two.

OBJECTIVE: To study the drug resistance of pseudomonas aeruginosa so as to provide references for rational use of antibiotics in the clinic.METHODS: The clinical distribution and the susceptibility test results of pseudomonas aeruginosa isolated from Beijing Tiantan Hospital in 2006 were analyzed retrospectively.RESULTS: The resistance rate of 334 strains to imipenem stood at 39.8%.The pseudomonas aeruginosa strains were mainly isolated from three important departments(neurological,respiratory and neurosurgery),the resistance rates to imipenem in the above three departments in descending order were as follows: department of respiratory(48.3%),department of neurological(40.2%),and neurosurgery(17.0%).CONCLUSION: The Pseudomonas aeruginosa isolated from Beijing Tiantan Hospital were highly resist to imipenem reduced and the strains isolated from different department varied in bacterial resistance,suggesting the drug resistance of antimicrobial surveillance should be emphasized in some key departments to keep the drug resistance under control.

This paper introduces the operational principle and structure of laser camera. The adjustment of image gray- scale and density is also mentioned

This paper discusses the limitations of traditional evaluation standard for digital medical images, and points out the reasons to select DQE as the new one. The differences of the two standards are compared and analyzed.

Objective To explore the possible mechanism of acute cerebral hemorrhage with systemic inflammatory response syndrome (SIRS) which caused multiple organ dysfunction syndrome (MODS), and to investigate the significance of serum nitric oxide (NO) and nitric oxide synthase (NOS) in the development of acute cerebral hemorrhage from SIRS to MODS.Methods The incidence rate of acute cerebral hemorrhage leading to SIRS and MODS was investigated. Serum NO and NOS levels in 73 patients with acute cerebral hemorrhage were detected dynamically by nitratase and chromometry methods. 20 normal healthy people were adopted as control group.Results The incidence rate of cerebral hemorrhage accompanied with SIRS was 47.95%(35/73),74.29%(26/35) of which leaded to MODS. 73 patients serum NO and NOS levels were elevated obviously, and the differences were significant compared with control group (all P

Objective To study X-ray and CT diagnosis and Differential diagnosis of nonossifying fibroma.Methods 29 cases of nonossifying fibroma proved by pathology were included in this paper.29 cases underwent radiography and 12 cases underwent CT scan.There were 22 male and 7 female,with age ranged from 5~59 years(mean age 21.3 years),X-ray and CT findings of tumors were analysed.Results The tumors were located in long bone in 27 cases,in which 17 cases were located in metaphses,2 cases in diaphyses,8 cases in bone end and 2 cases in irregular bone.The maximum diameter of tumors ranged from 0.5~7 cm.The tumors were round,oral or mild lobular in 21 cases,the tumors were showed sclerotic borders in 26 cases.Septum was demonstrated in 22 cases on X-ray plain films,but only 2 cases on CT.Bone expansion was showed in 13 cases.Conclusion Most of nonssifying fibroma have characteristic X-ray and CT findings,the diagnosis be identified by X-ray and CT before operation.

Objective To establish matched-pairs of DNA samples with copy-number controlled differential target genes, and to compare the detection sensitivity of typical Representational Difference Analysis (RDA) method and Subtractive Hybridization Difference Display (SHDD) method in isolating differential genes.Methods Two gene fragments (376 bp and 869 bp in length respectively) cloned by PCR using Human Papilloma Virus (HPV) DNA as template were used as differential target genes, and mixed with human genome DNA. Five matched, pairs of human genome DNA samples with gradually increased difference in copy numbers of target genes were established and RDA and SHDD methods were performed to clone differential target genes and compared their detection sensitivity. Results By using RDA method, 376 bp fragment with 6-fold difference and 869 bp fragment with 8-fold difference were cloned.However, both of these two target fragments with 4-fold difference were isolated using SHDD method.Conclusion The SHDD method adopts balanced bi-directional subtractive hybridization between two sample difference products and avoids loss of differential target genes caused by unbalanced subtractive hybridization of RDA method, and thus outweighs RDA method in isolating target genes, especially long-length target fragments, with small difference.

Objective To construct an infectious full-length cDNA clone of enterovirus 71(EV71)and develop a technological platform for study on vaccine development as well as molecular virology of EV71.Methods According to the nucleotide sequence of EV71 strain 085 isolated in China,four pairs of primers were designed for amplification of four end to end overlapping subgenomic cDNA fragments,the cDNA fragments were directional cloned into pBluescript SK(+)vector,and the virus genome cDNA clone was obtained by ligation orderly.The rescued virus of parental strain 085 from RNA transfected host cells was identified by RT-PCR,IFA,titration as well as transmission electron microscope(TEM)after the transcription of the full-length cDNA clone in vitro.Results The full-length cDNA clone was constructed successfully,and the typical CPE was observed after its transcription into Vero cells.The rescued virus with 20-30 nm in diameter can not only be neutralized by EV71 special anti-serum but also react with anti-EV71 monoclonal antibody that virus infected cells stained with FITC can be detected by IFA.After amplification from the total RNA extraction of virus infected cells by RT-PCR with EV71 special primers,the 226 bp products can be detected.The growth curve showed that the rescued virus can propagate in Vero cells stably with a titer of 4.5 ～6.0 lgCCID50/ml during 8 passages.The plaque formed by rescued virus is identical as parental virus in morphology but smaller in size.Conclusion An infectious full-length clone of EV71 was developed successfully,which will be used for further study on pathogenesis and vaccine development of EV71.

Animals ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Glomerulonephritis ; drug therapy ; pathology ; Humans ; Kidney Glomerulus ; pathology ; Phytotherapy ; Podocytes ; pathology