Objective To observe the clinical efficacy of captopril combined with simvastatin for treating patients with early diabetic nephropathy.Methods 180 patients with diabetic nephropathy were randomly divided into three groups,and each group had 60 cases,group A was given captopril for treatment,group B was given simvastatin and group C was received captopril and simvastatin for treatment.The clinical efficacy,renal function before and after treatment and incidence of adverse reactions were compared in three groups.Results The clinical efficacy of group C was significantly better than group A and group B ( P ＜ 0.05 ) ; after treatment,the value of Ma/Cr,SCR and Bun of three groups were lower than pre-treatment ( t =3.12,3.08,3.17,3.41,3.19,3.20,3.04,3.44,3.48,all P ＜ 0.05 ) ;and the value of Ma/Cr,SCR and Bun after treatment in group C were significantly lower than group A and group B ( t =4.58,4.69,5.02,4.89,5.11,4.87,all P ＜0.05 ) ;the incidence of adverse reactions in group C was significantly lower than the other two groups.Conclusion Captopril combined with simvastatin could significantly improve the clinical efficacy of the early diabetic nephropathy and had small side effects.

Objective To investigate the significance of levels of IL-8,TNF-α in serum of patients with aplastic anemia(AA).Methods Serum levels of IL-8 and TNF-α in 45 patients and 30 normal controls were measured by enzyme linked immunosorbent assay.Results The serum levels of IL-8,TNF-α increased significantly in AA and acute AA(AAA)group than in the control group(P＜0.05),there was no statistical significance between chronic AA(CAA)group and the control group(P＜0.05),and the levels in AAA group were much higher than in CAA group(P＜0.05).The serum level of IL-8 was positively related to TNF-αin AA group(r=0.682,P＜0.05).Conclusion There are immunological disorders in patients with AA and the overproduction of IL-8,TNF-α might be involved in the immunopathogenesis of AA,AAA.

A rapid and simple chromatographic procedure using HPLC-ECD is described for simultaneousdetermination of NE. E. DA. 5-HT with their precursor amino acid (Tyr. Trp) and their main metabolites (HVA. 5-HIAA). Using this assay,eight substrates are measured in cortex, diencephalon and brain stem of mice duing immunological response challenged by SRB6 3 days after challenged, the DA HVA in cortex, NE in diencephalon decrease obviously (p

A simple specific and sensitive radioimmunoassay for ne(?)ropeptide (NPY) in plasma and tissue extracts was developed. There was no appreciable cross-reactivity with related neuropeptides. The minimum detectable NPY in plasma and tissue extracts was 150-200pg/ml and average recovery was 85-90%. Reverse-phase HPLC reveals that chromatographic charecterization of NPY-Li in plasma and tissue extracts was very similar to that of standard NPY.

It has been found that Ginsenoside exhibits extensive pharmacological effects on animal and human.However,there have been few reports on these effects of Panax Quinquefolius saponin (PNS)so far.In this paper the immunologic effects of PNS were studied.Ⅰ.Immunologic effects of PNS on the immune cells of mice in vitror.The results showed that PNS could enhance ConA-induced splenocyte proliferation of mice,with the optimal concentration of lug/ml,The lymphokine(IFN,IL-2)production of splenocytes stimulated by ConA,and NKC activity of splenocytes also increadsed under the presence of PNS.Ⅱ.Effects of PNS on the immune function of mice in vivo The enhancement of spontaneous thymocyte proliferation,ConA-induced proliferation of splenocytes and thynocytes,ConA-induced production of IFN and IL-2 of splenocytes,NKC activity of splenocytes,and primary antibodyresponse to SRBC were examined.With the injection of PNS(1-2mg/20g/day)subcutaneously into mice for 7 days,

The specific binding of~(125)INPY to membranes from mouse cerebral cortex was investigated using equilibrium binding and kinetic assay.The membranes were preparated by different—speed centrifugation.The equilibrium binding of~(125)I—NPY to membranes at 37℃ to equilibrium was by 50 minute and showed sharp optimum at PH7.0—7.4.The bound,which can't inhibited by other related peptides,was inhibited by unlabled NPY spe—cifically(IC50=0.4nmoi/L).The binding sites for~(125)I—NPY were sensitive to treatment with tripsin and thiol reagents.Theequilibrium binding of~(125)I-NPY to cerebral cortex at 37℃ was characterized by a Kd value of 0.30—0.40nmol/L and the receptor densities were 0.40—0.45 pmol/mg protein.

The cDNA fragment of human c—fos oncogene has been obtained by a reverse transcription—DNA poly-merase chain reaction.Then the cDNA was ligated into pGEM3Zf(+)plasmid cut with Sma Ⅰ.with the re-combined plasmid,c—fos antisense RNA was synthesized in T7 RNA polymerase system.The primary appli-cation of anti-sense RNA is also studied in this article.

Objective To explore the surface molecule changes of the experimental autoimmune encephalomyelitis (EAE) rat brain vascular endothelial cells. Methods The expressions of vascular cell adhesion molecule\|1(VCAM\|1) and major histocompatibility complex (MHC) class Ⅱ molecules in the EAE rat brain vascular endothelial cells were tested by immunohistochemistry. The level of tumor necrosis factor (TNF) and interferon\|?(IFN\|?) in defferent clinic grade EAE were tested by MTT [3\|(4 5\|dimethythiazol\|zyl)\|2\^5\|diphenyl) tetrazolium bromide] method. Results There were inducible expressions of VCAM\|1 and MHC class Ⅱ molecules in the EAE rat brain vascular endothelial cells compared with control. The sera of EAE rats possessed the activities of TNF and IFN\|?.Conclusion\ In the CNS inflammation, the change surface molecules in the brain vascular endothelial cells may suggest that it plays important roles in EAE pathogenesis.\;[

Objective:To research the expression of HBsAg DNA vaccine in immunized mice.Methods:The expression of HBsAg DNA vaccine was detected by immunohistochemistry staining in muscle fibers,lymphoid nodes and spleens of mice after intramuscular injection.Anti-HBsAg antibody was assayed by ELISA and the proliferation of spleen cells was measured by MTT.Results:The positive expression was observed in the tissues mentioned above.The titer of serum antibody showed positive results.Spleen cells could be proliferated by stimulating with HBsAg or ConA.Conclusion:The positive expression and certain kinds of immune responses have been shown in immunized mice.

Objective:To investigate the role of sanchi Saponin Rg1 in D-galactose -induced amnesic mice ,and its possible mechanism.Methods:D-galactose was injected by subcutaneous to establish the aging model, and with drug treatment group.The Y-maze tests was performed to evaluate the learning and memory function, spectrophotometry was used to measure abilities of anti-oxidation.ELISA was adopted to evaluate the level of IL-1? and IL-6. Then FCM was applied to assay the index of intracellular calcium and the percentage of neural cell apoptosis.Results:Compared with drug treatment group, the model mice reduced significantly activites of anti -oxidation , the overload of intracelluer calcium and the increase in apoptosis of neural cells as well as the inflammation factor.Conclusion:Sanchi Saponin Rg1 decreased in the content of inflammation factor , the index of intracellular calcium and the percentage of apoptosis in neural cells , possibly by enchancing the antioxidant function.