Objective To observe the clinical efficacy of captopril combined with simvastatin for treating patients with early diabetic nephropathy.Methods 180 patients with diabetic nephropathy were randomly divided into three groups,and each group had 60 cases,group A was given captopril for treatment,group B was given simvastatin and group C was received captopril and simvastatin for treatment.The clinical efficacy,renal function before and after treatment and incidence of adverse reactions were compared in three groups.Results The clinical efficacy of group C was significantly better than group A and group B ( P ＜ 0.05 ) ; after treatment,the value of Ma/Cr,SCR and Bun of three groups were lower than pre-treatment ( t =3.12,3.08,3.17,3.41,3.19,3.20,3.04,3.44,3.48,all P ＜ 0.05 ) ;and the value of Ma/Cr,SCR and Bun after treatment in group C were significantly lower than group A and group B ( t =4.58,4.69,5.02,4.89,5.11,4.87,all P ＜0.05 ) ;the incidence of adverse reactions in group C was significantly lower than the other two groups.Conclusion Captopril combined with simvastatin could significantly improve the clinical efficacy of the early diabetic nephropathy and had small side effects.

Objective To investigate the significance of levels of IL-8,TNF-α in serum of patients with aplastic anemia(AA).Methods Serum levels of IL-8 and TNF-α in 45 patients and 30 normal controls were measured by enzyme linked immunosorbent assay.Results The serum levels of IL-8,TNF-α increased significantly in AA and acute AA(AAA)group than in the control group(P＜0.05),there was no statistical significance between chronic AA(CAA)group and the control group(P＜0.05),and the levels in AAA group were much higher than in CAA group(P＜0.05).The serum level of IL-8 was positively related to TNF-αin AA group(r=0.682,P＜0.05).Conclusion There are immunological disorders in patients with AA and the overproduction of IL-8,TNF-α might be involved in the immunopathogenesis of AA,AAA.

A rapid and simple chromatographic procedure using HPLC-ECD is described for simultaneousdetermination of NE. E. DA. 5-HT with their precursor amino acid (Tyr. Trp) and their main metabolites (HVA. 5-HIAA). Using this assay,eight substrates are measured in cortex, diencephalon and brain stem of mice duing immunological response challenged by SRB6 3 days after challenged, the DA HVA in cortex, NE in diencephalon decrease obviously (p

A simple specific and sensitive radioimmunoassay for ne(?)ropeptide (NPY) in plasma and tissue extracts was developed. There was no appreciable cross-reactivity with related neuropeptides. The minimum detectable NPY in plasma and tissue extracts was 150-200pg/ml and average recovery was 85-90%. Reverse-phase HPLC reveals that chromatographic charecterization of NPY-Li in plasma and tissue extracts was very similar to that of standard NPY.

It has been found that Ginsenoside exhibits extensive pharmacological effects on animal and human.However,there have been few reports on these effects of Panax Quinquefolius saponin (PNS)so far.In this paper the immunologic effects of PNS were studied.Ⅰ.Immunologic effects of PNS on the immune cells of mice in vitror.The results showed that PNS could enhance ConA-induced splenocyte proliferation of mice,with the optimal concentration of lug/ml,The lymphokine(IFN,IL-2)production of splenocytes stimulated by ConA,and NKC activity of splenocytes also increadsed under the presence of PNS.Ⅱ.Effects of PNS on the immune function of mice in vivo The enhancement of spontaneous thymocyte proliferation,ConA-induced proliferation of splenocytes and thynocytes,ConA-induced production of IFN and IL-2 of splenocytes,NKC activity of splenocytes,and primary antibodyresponse to SRBC were examined.With the injection of PNS(1-2mg/20g/day)subcutaneously into mice for 7 days,

The specific binding of~(125)INPY to membranes from mouse cerebral cortex was investigated using equilibrium binding and kinetic assay.The membranes were preparated by different—speed centrifugation.The equilibrium binding of~(125)I—NPY to membranes at 37℃ to equilibrium was by 50 minute and showed sharp optimum at PH7.0—7.4.The bound,which can't inhibited by other related peptides,was inhibited by unlabled NPY spe—cifically(IC50=0.4nmoi/L).The binding sites for~(125)I—NPY were sensitive to treatment with tripsin and thiol reagents.Theequilibrium binding of~(125)I-NPY to cerebral cortex at 37℃ was characterized by a Kd value of 0.30—0.40nmol/L and the receptor densities were 0.40—0.45 pmol/mg protein.

The cDNA fragment of human c—fos oncogene has been obtained by a reverse transcription—DNA poly-merase chain reaction.Then the cDNA was ligated into pGEM3Zf(+)plasmid cut with Sma Ⅰ.with the re-combined plasmid,c—fos antisense RNA was synthesized in T7 RNA polymerase system.The primary appli-cation of anti-sense RNA is also studied in this article.

Objective:To study the possibility that responses of fever and c- Fos expression in rat PVN and NTS to intraperitoneal administration of LPS are rnediated by vagal afferents. Methods: Rectal temperature was detected by digital temperature detecting instrument. c-Fos expressis was detected by immunohistochemistry staining.Results:The rectal temperature change value in vagotomy LPS gnoup was significantlydecreased compared with that in sham IPS group,and there was striking difference betwen them, p ＜0.05.It was increased significantlycompared with that in vagotomy NS group, P ＜ 0.05.The percentage of c-Fos positive neurons in rat PVN and NTS in vagotomy LPS group wassignificantly decreased compared with that in sham LPS group,and there were difference between them, p ＜0.01.Itwas stnkingly increased compared with that in vagotomy conrol group,respectively, P ＜0.01.Conclusion:The results indicate that vagus nerve is one of thepathways of peripheal LPS signal communicating to CNS.

Using hybridoma technique,17 hybridoma cell lines against astrocyte were ob-tained.The screening test of McAb showed:(1)Using immune enzymatic histochemistryfixed cell staining method all of 17 McAb reacted with astrocyte,(2) Using immuneenzymatic histochemistry viable cell staining method,eight of seventeen McAb react-ed with astrocyte.As to biological properties of McAb results were as follows:(1)Seven of eight McAb that showed positive reaction in viable staining assay have di-fferent degree of cytotoxicity to astrocyte,thymocyte and spleen cell in presence ofcomplement,one of eight has specific cytotoxicity only to astrocyte.(2) McAb(2C)possesses blocking effect on astrocyte,thymocyte and spleen cell proliferation respon-sive to various kind of mitogen.Furthmore,it has been found LPS may promote theproliferation of astrocytes and secreting interleukin 1(IL-1)-like substance This IL-1like substance seems to have a mutiple biological activities,i.e:(1)Thymocyte prolife-ration.(2) Fibroblast(L929 cell) proliferation and(3) Endogenous pyrogen activities.

Cultivation of murine thymic epithelium has been established.Murine thymic epi-thelial cultural supernatant showed activity increasing both spontaneous ~3H-TdR inco-rporation and ConA-stimulated proliferation of thymocytes by the exponent of 1.5-2at dilution 1:8 to 1:32,the peak of the activity was present at 10-20 or 15 days ofcultivation.However,the thymic epithelial supernatant from cultures at 6-7 daysshowed activity suppressing both proliferations of thymocytes at dilution 1:8-1:16.Theresult showed thymic epithelial supernatant had no 1L-2 activity.