Objective To explore if the expression of follicle-stimulating hormone ( FSH ) and its receptor ( FSHR) exist in the rat submandibular gland tissue , which may provide a theoretic evidence for further studying on adjusting function of the FSH in submaxillary gland .Methods Twenty normal SD rats , with a mean body weight of (200 ±20)g, were adopted to collect out the submandibular gland after abdominal cavity anesthesia .The method of in situ hybridization was employed on cellular localization of FSH and FSHR .RT-PCR was used to detect if FSH and its receptor mRNA exist in the rat submandibular gland .RNA was extracted from the submandibular gland , then FSH and the core cDNA of its receptor gene were obtained with reverse transcription polymerase chain reaction and analyzed on its sequence . Results The epithelial cells of serous acinus and granular convoluted tubule in submaxillary gland of rats had FSH and FSHR mRNA hybridized signals , which were detected in cytoplasm with negative nuclei .Specific bands of FSH cDNA and FSHR cDNA were identical to be 193bp and 413bp respectively.Conclusion FSH and FSHR can be synthesized by the epithelial cells of serous acinus and granular convoluted tubule in submaxillary gland of rats , thus this study further proves that the submandibular gland is the target organ of FSH .

Objective To investigate the diagnosis under hysteroscopy for uterine malformation,especially in maiden. Methods Hysteroscopy combined with ultrasonography was used to determine the types of uterine malformation in 24 cases. Results Definitive diagnosis indicated 8 cases of uterus bicornis,10 cases of uterus septus with single uterine neck,2 cases of uterus septus with double uterine neck and single uterine body,and 4 cases of puberal rudimentary horn of uterus. Conclusions Combined use of hysteroscopy and ultrasonography in the diagnosis of uterine malformation is accurate and applicable to maiden.

OBJECTIVE To investigate the method of quality control on the whole process in sterilization supply department. METHODS According to issued by Ministry of Health all kinds of criteria and inspections,the whole process in sterilization supply department was analyzed,and then the quality was controlled. RESULTS The whole process of sterilization was standardized,systematized and programmed. CONCLUSIONS The quality control on the whole process is an effective measure to ensure the rate of supply eligibility and support.

Objective To investigate the distribution of nerve growth factor(NGF) and epidermal growth factor(EGF) and their colocalization with follicle-stimulating hormone(FSH) in rat submaxilary glands,and to study the effects of FSH on the secretion of NGF and EGF by submandibular gland tissue in vitro.Methods Immunohistochemical colocalization method was used in the experiment.Submandibular tissues of rats were incubated in vitro and different concentration of FSH was added.Enzyme link immunoassay was used to measure EGF and NGF in supernatant.Results The serous glandular cells,granular convoluted epithelial cells and all other duct epithelial cells in the submandibular gland showed FSH,NGF or EGF positive immunoreactivity.The positive substance was distributed in the cytoplasm with negative nuclei.Both FSH and NGF or EGF positive products were co-located in rat submandibular gland.When the concentration of FSH was more than 10-5-10-6 IU/L,the secretion of EGF and NGF lessened with the decreased concentration of FSH;when the concentration of FSH was less than 10-5-10-6 IU/L,the secretion of EGF and NGF increased with the decreased concentration of FSH.Conclusion FSH has the same bidirectional regulation effects on EGF and NGF in vitro.FSH may regulate the function of endocrine of rat submandibular gland.

[Objective]To explore the traditional processing techniques and Jingbang(Beijing local)experience of Ophiopogon japonicus,providing a reference for modern research on Opehiopogon japonicus.[Methods]Collecting data on the processing of Ophiopogon japonicus from ancient herbal literature and traditional Jingbang heritage materials,and consulting with old pharmacists,based on the analysis and organization,the various processing methods of Ophiopogon japonicus were restored.[Results]The purification methods of Ophiopogon japonicus have mechanisms such as removing the pith to relieve annoyance,removing the pith to treat channel dryness,and removing the pith to nourish lung Yin,with the first one being the most widely spread.The methods of removing the pith include boiling water to moisten the pith,clear water to moisten the pith,and roasting to soften the pith,with the first one being the most effective.The purpose of the wine soaking method is to alleviate the side effects of long-term use of Ophiopogon japonicus on stomach cold.The rice frying method should spread the rice thinly in the pot,spread Ophiopogon japonicus on the rice,and should not be stirred.If it is stirred with the rice,Ophiopogon japonicus is easy to puff up.The steaming method has two types:clear steaming and mixing with milk steaming.Ophiopogon japonicus is not easy to crush,and the intermittent roasting method recorded in Compendium of Materia Medica is very good.The method of simmering Ophiopogon japonicus and making Ophiopogon pills both utilize its juice-containing characteristics.The traditional Jingbang Ophiopogon japonicus has obvious local characteristics in the methods of moistening and removing the pith.Zhu Ophiopogon japonicus is for sprinkling water and moistening slightly,and cinnabar fine powder is finely scattered with sprinkling and stirring.These two processed varieties have disappeared for half a century.[Conclusion]The restoration and optimization of the processing methods of Ophiopogon japonicus are expected to improve the efficacy of Ophiopogon japonicus,providing higher quality Ophiopogon japonicus medicinal materials for the clinic.It reveals the local characteristics of the traditional Jingbang Ophiopogon japonicus processing,providing a possibility for the revival of the disappeared processed varieties,which is of great significance for enriching Chinese medicine resources and enhancing the clinical application value of Chinese medicine.

Objective To understand the compliance of statins and their influencing factors in elderly patients with hyperlipidemia,in order to provide a basis for the improvement of medication compliance. Methods A total of 120 patients who were diagnosed of hyperlipidemia in the elderly ward of a Class Ⅲ hospital in Beijing from January to November 2016 were selected by convenient sampling method and investigated with questionnaire about general information and medication compliance.Results There were 62 cases (51.67％) with good compliance,and 58 cases (48.33％) with poor compliance in total. The result of univariate analysis showed that there were significant differences in patients′ medication compliance with different age,education degree and living style (P<0.05). Logistic regression analysis showed that advanced age,low education level,and living alone were the risk factors of poor medication compliance.Conclusions Medical staff should pay more attention to the elder patients who were living alone and with low education level. It is suggested taking family members into the management of patients′ disease and medication,and taking measures to improve the compliance of statins in elderly patients with hyperlipidemia.

Objective:To study the safety factors of Fuyanyu mixture. Methods: The contents of antiseptic and residual ethanol were determined by HPLC and GC, respectively, and those of heavy metals and harmful elements were detected by ICP-MS. Results:The contents of benzoic acid in 6 batches of samples were less than 0. 3%. In two thirds of samples, ethanol residue was more than 0. 5%. The total mercury exceeded 15μg/day in one of the samples, and the contents of Pb, Ge, Gr, As, Ni and Cu met the limits described in USP<232> in all of the last samples. Conclusion: The methods are accurate and reliable. It is urgent to improve the preparation process so as to reduce the residual amount of ethanol according to the detection results. It is recommended to increase the testing items that affect the safety of the preparation so as to control the preparation quality strictly.

Objective:To study the safety factors of Fuyanyu mixture. Methods: The contents of antiseptic and residual ethanol were determined by HPLC and GC, respectively, and those of heavy metals and harmful elements were detected by ICP-MS. Results:The contents of benzoic acid in 6 batches of samples were less than 0. 3%. In two thirds of samples, ethanol residue was more than 0. 5%. The total mercury exceeded 15μg/day in one of the samples, and the contents of Pb, Ge, Gr, As, Ni and Cu met the limits described in USP<232> in all of the last samples. Conclusion: The methods are accurate and reliable. It is urgent to improve the preparation process so as to reduce the residual amount of ethanol according to the detection results. It is recommended to increase the testing items that affect the safety of the preparation so as to control the preparation quality strictly.

PURPOSE: This study compared the survival outcomes of different treatment methods for the ipsilateral breast of occult breast cancer (OBC) patients with axillary lymph node metastasis. METHODS: A retrospective study was conducted in which forty OBC patients with axillary lymph node metastasis were identified out of 15,029 patients who had been diagnosed with a primary breast cancer at between 1992 and 2010. The patients were categorized into three treatment groups based on ipsilateral breast management: breast-conserving surgery (BCS) (n=17), mastectomy (n=12), and nonsurgical intervention with or without radiation therapy (No surgery with or without radiation therapy [No Op+/-RT]) (n=11). All patients underwent axillary lymph node dissection. Cases were evaluated based on treatment and potential prognostic factors with respect to overall survival (OS) and disease-free survival (DFS). RESULTS: During the follow-up period (median follow-up of 71.5 months), the overall OS and DFS were 76.9% and 74.9%, respectively. The 5-year treatment-specific OS was 72.0% for the BCS group, 74.0% for the mastectomy group, and 87.5% for the No Op+/-RT group (log-rank p=0.49). The 5-year DFS was 70.6% for the BCS group, 66.7% for the mastectomy group, and 90.9% for the No Op+/-RT group (log-rank p=0.36). Recurrence rates for the BCS and No Op+/-RT groups were 5.9% and 18.2%, respectively. Histologic grade and lymph node status were inversely correlated with DFS (log-rank p=0.04 and p<0.01, respectively). CONCLUSION: There was no difference in survival outcomes between the three treatment methods for the ipsilateral breast (mastectomy, BCS, and No Op+/-RT) of OBC patients with axillary lymph node metastasis. A large-scale multicenter study is needed to validate the results from this small retrospective study.
Breast Neoplasms* ; Breast* ; Disease-Free Survival ; Follow-Up Studies ; Humans ; Lymph Node Excision ; Lymph Nodes* ; Mastectomy ; Mastectomy, Segmental ; Methods* ; Neoplasm Metastasis* ; Recurrence ; Retrospective Studies

PURPOSE: The aim of this study was to evaluate outcomes of a total colectomy with ileorectal anastomosis in patients with slow transit constipation. METHODS: A retrospective review of 37 consecutive patients with slow transit constipation who underwent a total colectomy between 1994 and 2008 was undertaken. Preoperative and postoperative Wexner's constipation scores were collected and used to evaluate the outcomes after surgical treatment. Also patients' postoperative satisfaction scores were collected using a 4-point scale. RESULTS: The 37 patients consisted of 31 women and 6 men, with a median age of 41 years (range, 17 to 71 years). Pre- and post-operative Wexner's scores were collected from 33 patients (89.1%), and the mean preoperative Wexner's score was 19.3 (range, 11 to 24), which decreased to an average post-operative score of 2.3 (range, 0 to 8). Neither intraoperative complications nor postoperative mortalities were noted. Five patients (13.5%) had early postoperative complications, and the most common complication was postoperative ileus (10.8%). Seven patients (18.9%) had late postoperative complications, and postoperative ileus (10.8%) was also the most common. Twenty seven of 33 patients were satisfied with their surgical outcome (81.8%). CONCLUSION: A total colectomy with ileorectal anastomosis might be an effective surgical procedure with acceptable morbidity to treat medically intractable slow transit constipation.
Colectomy ; Constipation ; Female ; Humans ; Ileus ; Intraoperative Complications ; Male ; Postoperative Complications ; Retrospective Studies ; Treatment Outcome