The gonadotropin and testosterone secretion of 20 normal men and 2 patients with IHH was investigated by pulsatile-subcutaneous administration of LHRH with a single dose of 5?g or 10?g per pulse in the former and 5?g per pulse at 90-min intervals in the latter. Blood samples for FSH, LH and T assays were drawn before and at 15, 30, 45, 60 and 90min after first pulse in normal men and one patient and on the 8th treatment day in the other patient. To observe the dynamic changes in hormone responses to pulsatile LHRH treatment in IHH, blood samples were also taken weekly aV interpulse period just before subsequent pulse. In normal men following a pulsatile injection of LHRH, the peak serum LH levels rose to 2.5 and 2.6 times as much as the basal at 30 min (5?g LHRH) and 45 min (10?g LHRH) respectively, peak FSH levels were elevated to 1.55- and 1.94-fold of the basal at 45 min, while only a tendency tp increase in T concentration was noted. No statistical significance was observed in LH, FSH and T levels between different, doses tested at any phase after LHRH administration in normal men. In IHH, LH, FSH and T levels showed no response after first pulsatile LHRH treatment yet they were increased significantly on the 8th day of treatment. The methodology of pulsatile administration of LHRH by portable pump and the relationship between the doses tested and their responses were discussed.

Objective To discuss the technique, therapeutic effect and complications of CT-guided interstitial implantation of ~(125)I seeds for the treatment of non-small cell lung carcinoma (NSCLC). Methods With the help of treatment planning system, CT-guided interstitial implantation of ~(125)I seeds was performed in 21 patients with pathologically-proved NSCLC. Multi-plane implanting technique was adopted and ~(125)I seeds of (0.5 -0.8) mCi were placed at intervals of (1.0-1.5) cm. Checkup CT scan was carried out immediately after the procedure to make sure that the seeds were satisfactorily distributed. Follow-up CT scanning was made (2-6) months after the treatment to survey the distribution of the seeds within the tumor. The curative effect and complications were also evaluated and observed. The patients were followed for 12 months. Results Of 21 cases, complete remission was seen in 7, obvious remission in 12 and partial remission in 2. The one year survival rate was 90.5% (19/21). Complications included pneumothorax (n = 6) and hemoptysis (n = 2). No serious complications or treatment-related radiation injuries occurred. Conclusion CT-guided interstitial implantation of ~(125)I seeds is a safe and effective treatment for NSCLC with reliable short-term therapeutic effect.

[Objective] To observe the effect of HQSM on PGI_2/TXA_2 in blood plasma of acute myocardial ischemia rats caused by injecting pituitrin. [Methods] 84 SD rats were divided into seven groups at random: normal control group,model control group,three HQSM groups(210mg/kg,150mg/kg,105mg/kg),Huangqi Shengmaiyin and DI’ao Xinxuekang. Intragastric administration was adopted once a day for 30 days.Acute myocardial ischemia model rat was established by injecting pituitrin from tail vein 1h after the last administration, and blood plasma was separated to be determined the activity of 6-Keto-PGF_ 1? and TXB_2, which is the metabolite of PGI_2 and TXA_2 in blood plasma of SD rats. [Results] Having been injected pituitrin,the activity of TXB2 upgraded while 6-Keto-PGF_ 1? /TXB_2 decreased(P

[Objective] To study synergistic effect and decreasing toxicity of Sarcandra Glabra essential oils (SGE) on H22 and S180 bearing mice treated with cytoxan (CTX).[Method] Transplanting H22 and S180 mice tumor models were established to observe the tumor inhibiting rate when SGE was used in combination with CTX by two different routes of administration.While the inhibition effects of SGE in decreasing blood platelet,leukocyte and hemoglobin induced by CTX were also detected.[Result] SGE has anti-tumor and synergy effect when combined with CTX on H22 and S180 bearing mice.The H22 and S180 tumor inhibiting rates of CTX (10mg?kg-1 or 15mg?kg-1) combined with SGE (36.6mg?kg-1) by iv.were increased compared with using CTX alone (P

Object To probe into the antihypertension activity mechanism of extracts from Compound Jueming (CJ). Methods The method of radioimmunoassay was used to assay the concentration of the related substances on spontaneously hypertensive rats (SHR) such as angiotensin Ⅱ (ANGⅡ), renin activity (RA), endothelin (ET), atrial natriuretic polypeptide (ANP), aldosterone (ALD), and urine. Results All of the three doses (9.5, 7.2, and 4.8 g/kg) of extracts from CJ reduced ANG Ⅱ and RA, but had no influence on ALD, ET, ANP, and urine in SHR. Conclusion The antihypertension effect of extracts from CJ is related to decreasing of renin angiotensin, but not to ALD, ET, ANP, and urine.

AIM To study effect of sodium ferulute on experimental arrhythmias. METHODS Arrhythmias were induces by drugs and myocardial ischemia. RESULTS Sodium ferulute 0 6 g?kg -1 iv antagonized arrhythmias induced by ouabain, adrenalin and myocardial ischemia( P

AIM: To observe the inhibitory effects of the serum containing drugs of Qinbai Qingfei Concentrated Pellets(S-QQCP) on influenza A virus. METHODS: MDCK cells were cultured with the technology of cell culture in vitro,serum contained QQCP of low-,moderate-,high dosages were made with Serum Pharmacological methods,in order to study the action of S-QQCP inhibit the biosynthesis,absorption and direct destruction of influenza A virus. RESULTS: Compared with the controlled blank serum,different dosages of S-QQCP had significant inhibitory effects on the biosynthesis of influenza A virus(P0.05). CONCLUSION: S-QQCP can inhibit the biosynthesis and absorption of influenza A virus to a certain extent.

Objective To investigate the condition of activity and participation of patients with traumatic brain injury (TBI). Methods 34 inpatients with TBI were assessed with World Health Organization Disability Assessment Schedule (WHO-DAS) 2.0 (International Chinese Version of fully structured interviewer administered 36 question). Results The patients with TBI had mild difficulty (2.10±1.33) in understanding and communicating, moderate in getting around (3.20±1.56), self-care (3.06±1.41) and getting along with people (2.43±1.33),and moderate to severe in life activities (3.86±1.33) and participation in society (3.51±1.19). Conclusion The patients with TBI feel difficulty major in life activities, participation in society, and getting around.

Objectives: To establish melanoma models of B16 cell line in ICR mice. Methods: Inoculating B16 Melanoma cells in ICR mice's feet, back, abdomen and caudal vein, to establish transplanted and metastatic melanoma models. We observed the morphologic characteristics and examined the pathological parameters of melanoma. We analyzed the relations between the number of B16 cells inoculated and the genesis of tumors, the influence of tumor growth on mice's behavior,and growth curve of the melanoma. Results:Six days later, sesamoid, linear and nodal even kaulifloweroid melanoma were seen gradually in ICR mice's feet, back, abdomen and lung (anatomid) after inoculating. The bearing tumor rates have a positive relation to the number of inoculated B16 cells. The rates of bearing tumor was near 100%, after inoculating with 5?10 6 B16 cells. Conclusions: The B16 melanoma cell line derived from C57/B16 mice(black ) has high rates of bearing tumor in ICR mice (white). The B16 melanoma models in ICR mice are ideal tumor models that have the characteristics of easy established,easy observing and easy obtaining.

Objective To determine the effect of atorvastatin on the hypercholesterolemia in-duced lesion in the lung. Methods Fifteen male New Zealand rabbits were randomly assigned into a control group (n=5) , a high-cholesterol forage group (n=5) , and an atrovastatin treatment group (n=5). The control group received normal forage, but the high-cholesterol group and atrovastatin treatment group received high-cholesterol forage. From the 9 th week, the atrovastatin treatment group was added atorvastatin, and the experiment stopped at the end of the 14th week. At the beginning of the experiment and at the 8 th, 14 th week, blood cholesterol and body weight were detected. At the 14th week, bronchial alveolar lavage (BAL) was performed in vitro after the rabbits were executed; pathological examinations were determined in the lung tissues by staining with hamatoxylin-eosin. Oil red 0 and the activities of NF-κB in the alveolar macrophages (AMs) were investigated by immuno-cytochemistry. Proliferative cell nuclear antigen in the lung tissues was adopted by immunohistochem-istry, and the concentrations of IL-6 in the serum, BALF and the culture supematants of AMs were measured by ELISA. Pulmonary tissue paraffin section was stained with hamatoxylin-eosin. Results Atorvastatin reduced inflammatory infiltration, AM NF-κB activation, and cell proliferation in the lung, but raised IL-6 level. Conclusion Hypercholesterolemia-induced pulmonary inflammation is attenuated by atorvastatin.