1.Practice and enlightenment of cardio-pulmonary resuscitation emergency training
Chinese Journal of Medical Education Research 2015;(12):1286-1289
Objective Based on the international standard cardio-pulmonary resuscitation curriculum, the aim of the study is to investigate the effectiveness and importance of the layered cardio-pulmonary resuscitation emergency training. Method A total of 219 trainee including clinical medical personnel , auxiliary medical personnel and medical staff with standardized training were enrolled . Training included watching course video, team practice and class discussion, and the theoretical score and operation marks before and after layered cardio-pulmonary resuscitation training were compared. Theoretical score were compared by paired T-test, and operation pass ratio was determined byχ2 analy-sis. Results The pre- and post-training theoretical score of medical staff with standardized training, auxiliary medical personnel, and clinical medical personnel were 66.60±7.82 vs. 88.60±6.37;61.60± 7.44 vs. 86.90±5.80;73.45±6.83 vs. 94.75±5.04 respectively. The pre- and post-training operation pass ratio of marks of medical staff with standardized training, auxiliary medical personnel, and clinical medical personnel were 31.1%/85.2%;32.0%/90.7%;59.0%/96.4% respectively. Conclusion There are differences in both the theory and operation results of medical personnel at different levels before and after the training of cardiopulmonary resuscitation. The more targeted and layered training is much effective in cardio-pulmonary resuscitation emergency training.
2.High-dose epirubicin combination therapy in the treatment of 48 cases with advanced chest malignant tumors
Weiguang CHEN ; Zhengxing YANG ; Guitao DENG
China Oncology 1998;0(04):-
Purpose:To estimate the curative effects and side effects obtained from high-dose epirubicin (EPI) combination therapy in the treatment of advanced chest malignant tumors. Methods:Forty-eight cases with advanced chest malignant tumor(32 cases with non-small-cell lung cancer; eleven cases with breast cancer and five cases with mediastinal malignant lymphomas) were treated with the combination therapy of high-dose EPI. Results:The response rate in non-small-cell lung cancer was 56.3%, in breast cancer was 72.7%, in mediastinal malignant lymphomas was 100%. The most frequent toxicities were neutropenia.Conclusions:The combined chemotherapy of high-does EPI is an effective and safe treatment in advanced chest malignant tumor.
3.HLA-DRB1 genotyping and its relation with chronic hepatitis B p atients of Hanethnic in Shanxi Area with HBV infection
Guitao YANG ; Jie LIU ; Dezhong XU
Medical Journal of Chinese People's Liberation Army 2001;0(10):-
Objective To study the HLA-DRB1 genotype and their r elation with HBV infection among Han patients in shanxi area. Methods HLA-DRB1 genotyping was conducted in 54 patients HBV infection and 108 health controls, as well as 32 asymtomatic HBsAg carriers by using polymerase ch ain reaction/sequence specific primer (PCR-SSP) method. All the patients, asy mtomatic HBsAg carriers, and healthy subjects were inhabitants of Shan'xi area o f Han nationality. The association between HLA-DRB1 genotype and different repl ication of HBV were also studied. Results DRB1*04,DRB1*09,DRB 1*12, DRB1*15 were the most common genotypes in the Shan'xi Han inbabitants wit h the frequency of 16.2%,12.5%,11.6% and 13.4% respectively. Compared to 108 healthy controls, the allele frequency of HLA-DRB1*03 was 11.1% in HBV patien ts versus 3.7% in healthy controls, with odds ratio=3.57 and Pc=0.014 ( P
4.Experiment on graft of bone marrow-derived neural stem cells in peripheral nerve
Guitao LI ; Ruxiang XU ; Xiaodan JIANG ; Zhijun YANG ; Guanghui DAI ; Zhenzhou CHEN ; Tao HUANG
Chinese Journal of Tissue Engineering Research 2005;9(38):187-189
BACKGROUND: Since there are only cell axons of neurons in peripheral cells, the study on neural stem cells (NSCs) is almost focused on neuronal cells, for which, the study on repair of peripheral nerve may be based on some experiences in NSCs.OBJECTIVE: To observe the repair of peripheral nerve after graft of autologus bone marrow derived NSCs in the injured area. To observe whether the grafted NSCs were survived and migrated in spinal cord as differentiated neurons in the injured area of peripheral nerve or not.DESIGN: Observed controlled experiment was designed.SETTING: Institute of Neurological Medicine of Zhujiang Hospital affiliated to Southern Medical UniversityMATERIALS: Eight New Zealand big white rabbits were employed, of clean grade, mass weighted varied from 1.5 to 2.5 kg and of either sex.METHODS: The experiment was performed in Institute of Neurological Medicine of Zhujiang Hospital affiliated to Southern Medical University collected from New Zealand big white rabbits for culture and differentiation was prepared. Sciatic neural injured area of one side was randomized as graft side. Physiological saline, collagen matrix and cellular embedding solution were infused up to 0.01 mL (containing stem cells 1×1010L-1). Another side was taken as the control, in which, collagen matrix suspension 0.01 mL was infused. Peffusion and fixation were followed 3 months after graft and auto-graft was performed in the injured peripheral nerve. The materials were collected for observation from graft area, spinal cord area, injured area on the opposite side and normal neural area.MAIN OUTCOME MEASURES: Morphology of nerve fibers and neuronal cells in NSC graft area, spinal cord area and non-graft area on opposite injury side.RESULTS: The density and continuity of nerve fibers grown in graft area were higher remarkably than non-graft area on opposite side and more Schwann cells were seen under optic microscope. With amplified ×400 visual field, Ranvier's node of spinal nerve fiber was visible. In addition,mucous matrix and few fibroblasts were seen also in the space of nerve fibers. The survived neuronal cells were no visible in graft area, spinal cord area and non-graft area of sciatic injury on the opposite side.CONCLUSION: Graft of autologus bone-marrow derived neural stem cells in defect area of peripheral area benefits repair of nerve fibers. But the neural stem cells cannot survive as neurons in graft area of peripheral nerve, spinal cord area and the defect control on the opposite side.
5.Pathological evaluation of immune system in drug safety study
Zhi LIN ; Jianjun LV ; Zhe QU ; Guitao HUO ; Di ZHENG ; Yanwei YANG ; Xue WANG ; Bo LI
Drug Evaluation Research 2017;40(1):1-4
The immune system is a complex system involving multiple organs,and it is vulnerable to age,gender,environment and other factors.For a variation normal physiological range,it is a great challenge to evaluate drug-induced immunotoxicity in preclinical safety study.Histomorphologic assessment of the immune system is a recognized cornerstone in the identification of immunotoxicity at present.In this paper,the principles of pathological evaluation for immune system,and pathological evaluation for important immune organs including thymus,spleen,lymph nodes are discussed briefly,so that it is intended to assist toxicity pathologists in the accurate and consistent characterization of intended and unintended drug-induced alterations of the immune system.
6.HLA-DRB1 genotyping and its relation with chronic HBV infection in patients
Guitao YANG ; Jie LIU ; Dezhong XU ; Yongping YAN ; Huahong XIE ; Yulei DU
Chinese Journal of Pathophysiology 1989;0(05):-
AIM: To study the HLA-DRB1 genotype and their relation with HBV infection in Shaanxi Han patients.METHODS: HLA-DRB1 genotyping was conducted in 108 case of chronic HBV infection and 108 health controls as well as 32 asymtomatic HBsAg carriers by using polymerase chain reaction-sequence specific primer method.All the patients,asymtomatic HBsAg carriers and health subjects were residents of Shaanxi district and belonged to Han nationality.The association between HLA-DRB1 genotype and different replication of HBV was also studied.RESULTS: DRB1~*04,DRB1~*09,DRB1~*12 and DRB1~*15 were the most common genotypes in Shaanxi Han residents with the frequency of 16.2%,12.5%,11.6% and 13.4%,respectively.Compared to 108 health controls,the allele frequency of HLA-DRB1~*03(10.6% of HBV patients versus 3.7% of health controls,odds ratio=3.10;P
7.Investigation and analysis of clinical skill training course
Haijuan WANG ; Weiyun BI ; Aixia SONG ; Lang LI ; Shanshan PU ; Qiang XUE ; Guitao YANG
Chinese Journal of Medical Education Research 2013;(12):1267-1270
Objective To improve the clinical skill training courses in order to meet the new challenges and different requirements. Methods Totally 327 clinical physicians of different levels in 36 departments were enrolled and 286 physicians were randomly surveyed with the questionnaire , including subjects' general characteristics, participation rate, training courses and training model. Results Among the trainees, most of them were attending physician and resident physician, and their participation rate was 88.5%(77/87) and 90.0% (172/191) respectively. The most popular and well acknowledged training courses were emergency treatment course such as AHA basic/advanced life support course. Training model like ‘group class, one topic, one hour’ was well accepted by 84.1%(275/324) physicians and course time of 16:30-17:30 was preferred by 65.5% (214/327) physicians. Conclusions A series of most popular and significant training courses are developed. More important-ly, physicians' real need and new expectation to the training course are well recognized, which is im-portant to plan the further training program and courses.
8.Toxicological pathology in preclinical drug safety evaluation
Zhi LIN ; Jianjun LV ; Guitao HUO ; Di ZHANG ; Yanwei YANG ; Shuo ZHANG ; Xue WANG ; Bo LI ; Zhe QU
Drug Evaluation Research 2017;40(4):450-454
Toxicological pathology is a morphological elucidatet ion of tissue damage caused by drug toxicity,including damage type,location,severity grade and prognosis.It provides objective and accurate data to support preclinical drug safety evaluation.Therefore,toxicological pathology plays an important role in drug safety evaluation,and the pathologic data or conclusion often determines the termination or continuation in many drug development projects.In this paper,the characteristic of toxicological pathology,standardized management,the development of new techniques including in situ hybridization,laser scanning cytometry and laser capture microdissection,and related problems in pathology are discussed briefly.
9.Analysis and strategy research on the simulation teaching of airway management in teaching hospitals in China
Ru WANG ; Aixia SONG ; Lang LI ; ShanShan PU ; Weiyun BI ; Haijuan WANG ; Guitao YANG
Chinese Journal of Medical Education Research 2018;17(4):429-432
With the development of science and technology,medical simulation has been applied extensively.The application of simulation technique in teaching hospitals has become a new choice in modern medical education.As the most basic and important clinical ability,airway management has become a popular training program in China's teaching hospitals.This paper summarizes the current situation of the simulation training of airway management in teaching hospitals at home and abroad,concludesthe existing problems in the simulation teaching of airway management of teaching hospitals in China,such as narrow range of training objects,lack of professional teaching staff,unscientific curriculum design and outdated training model,etc.Then puts forward the countermeasures of expanding the training coverage,establishing the professional teaching team,designing the curriculum design of science,and introducing advanced simulation equipment to improve the quality of simulated teaching.
10.Application of the Living Lab concept combined with the case-based study and reference-induced self-education model in thoracic surgery nursing teaching
Yan LU ; Shuwen LI ; Ruituan LIAN ; Guizhen YANG ; Huimin WU ; Guitao WANG
Chinese Journal of Medical Education Research 2023;22(9):1413-1416
Objective:To investigate the application effect of the Living Lab concept combined with the teaching model of case-based study (CBS) and reference-induced self-education (RISE) in thoracic surgery nursing teaching.Methods:A total of 132 nursing students who participated in 11 rounds of training (12 nursing students per round) in Department of Thoracic Surgery, The Affiliated Hospital of Inner Mongolia Medical University were included in the study, and according to the teaching method, they were divided into control group and observation group, with 66 nursing students in each group. The students in the control group received routine teaching, and those in the observation group received the Living Lab concept combined with CBS-RISE teaching. Assessment results were compared between the two groups, and the critical thinking ability and degree of satisfaction with teaching were evaluated for both groups. SPSS 22.0 was used to perform the t test. Results:Compared with the control group, the observation group had significantly higher objective question score (48.82±4.21 vs. 46.73±4.54, P<0.05), subjective question score (33.12±3.86 vs. 31.59±3.47, P<0.05), and total score (81.02±7.57 vs. 76.34±7.91, P<0.05). After training, compared with the control group, the observation group had significantly higher total score and scores of the dimensions such as truth seeking, open mindedness, analyticity, systematicity, self-confidence, inquisitiveness, and cognitive maturity ( P<0.05). The observation group also had a higher degree of satisfaction with teaching methods, teaching contents, and teachers compared with the control group ( P<0.05). Conclusion:In thoracic surgery nursing teaching, the application of the Living Lab concept and CBS-RISE can improve the assessment results of nursing students and enhance their critical thinking ability, with a high degree of teaching satisfaction among nursing students.