In order to explore the possibility and feasibility of administering antisense oligodeoxynucleotide to vascular adventia delivering by fibrin tissue adhesive, the authors first observed the physical characteristics of the adhesive, then the blue dye (methylenophin) was dissolved in the adhesive and the color change of the supernate was monitored. The adhesive with antisense oligomer inside was immerged in saline and the optical density (OD) value of the released oligomer was measured by ultraviolet spectrophotometer at different time points, then the in vitro controlled releasing curve was made. The preliminary in vitro test found the adhesive clot in 10 seconds when the two kinds of solution were mixed together, and the surface was membrane like under microscope, the clot inside was silk protein like configuration and was filled up with particle like liquid. The clot was elastic and had some reabsorbable property like that of sponge. The blue dye inside the clot gave a more and more significant dyeing of the supernate with time going. The in vitro releasing curve indicated that most oligomer was released within 72 hours. This implied that the adhesive clot is like a sponge reservoir of drugs, it could elongate the action time of antisense drug at vascular adventia.

0 05). The results suggest antisense C myc oligodeoxynucleotide significantly suppressed the intimal hyperplasia in anastomosis by local fibrin glue. The investigation provides the basis for the early clinical trials of antisense C myc for the prevention of restenosis after anastomosis.

The effects of octreotide on DNA synthesis and the expression of endogenous insulin like growth factor I(IGF I)in newborn calf vascular smooth muscle cells(VSMCs) in vitro were analysed with 3 H thymidine incorporation and immunocytochemistry respectively.Concentration over 1 nmol/L of octreotide inhibited the increasing of 3 H thymidine incorporation induced by serum and IGF I in VSMCs. Serum and IGF I could stimulate the synthesis of endogenous IGF I in VSMCs.When cultured with 10 nmol/L of octreotide,the gray value of IGF I rose 16 0% and 13 3% respectively.It is suggested that octreotide can inhibit DNA synthesis induced by serum and IGF I in VSMCs,and the expression of endogenous IGF I in VSMCs.

Two stage polymerase chain reaction(restricted fragment length polymorphism was used to study K ras mutations and chemiluminescence immunoassay was used to detect CA19 9 in plasma from patients with pancreatic adenocarcinoma, some samples were analyzed by direct seqencing. The results showed 18 (60%) of 30 patients with pancreatic adenocarcinoma had K ras mutations in plasma, and 25 (83 3%) of 30 had elevated CA19 9 in plasma. The former was less sensitive than the latter, but the diagnostic rate of combined analysis was 100%. It is concluded that combined analysis of molecular tumor markers and protein tumor marker is more sensitive than protein tumor markers alone, it might overcome Lewis blood type limitation to protein markers.