Background and purpose:Extraovarian peritoneal serous papillary carcinoma(EPSPC) originats from peritoneum with rare incidence, sometimes may affect the surface of ovary and have multi-focal lesions, whose standard therapy have not been established. In this study, we preliminarily reviewed the clinical characteristics, treatment and prognosis of EPSPC in 11 cases. Methods:The clinical characteristics of 11 cases with EPSPC were first retrospectively analyzed. All the patients underwent cytoreduction surgery followed by chemotherapy. Finally, short-term and long-term effi cacy, time to progression (TTP) and overall survival were evaluated by RECIST, respectively. Results:Abdominal pain, distention and ascites were the most common presenting symptoms, but tumors could be palpable in only 18.2% of the patients. The positive rate of ascites, abdomen B ultrasound,MRI scan and ascending CA125 level in the plasma and ascites was 100%,45.5%,100%,72.7%,81.2%,respectively. The successful rate of cytoreduction surgery was 45.5% for the EPSPC. After chemotherapy, the cases of complete remission, partial remission, stable disease and progression disease were 1(11.1%,1/9),3(33.3%,3/9),2(22.2%,2/9) and 3(33.3%,4/9), respectively. TTP was 5-14 months for all the patients and the median TTP was 8.6 months. The 1,2,3-year overall survival was 72.7%,18.2%,0%, respectively and the median overall survival was 14.6 months. Conclusions: Ascite, abdomen MRI scan and CA125 level are the most meaningful factor to diagnose EPSPC. EPSPC is a carcinoma of poor prognostic with non-specific clinical characteristics, low successful rate of cytoreduction surgery and is chemotherapy-resistent.

Objective To observe the effects of mouse indoleamine 2,3-dioxygenase (IDO) gene on CD4+ T cells by transfecting an eukaryotic expression vector containing mouse IDO gene fused with enhanced green fluorescent protein (pEGFP-N1). Methods The immature dendritic cells (imDCs) derived from mouse bone marrow were cultured in vitro and morphologically identified by transmission electron microscope, scanning electron microscope and flow cytometry. The immature dendritic cells were transfected by DOTAP liposome, and the expression of green fluorescence protein was confirmed with inverted fluorescence microscope. The spleen-derived CD4+ T lymphocytes from mice were isolated and purified in vitro, and then carried to mixed lymphocyte reaction (MLR) with 3H-TdR incorporation. The effect of IDO gene on the proliferation of spleen-derived CD4+ T lymphocytes of the allergized mouse challenged by ovalbumin was assayed by 3H-TdR MLR incorporation, and the apoptosis of spleen-derived CD4+ T lymphocytes was assayed by TUNEL. Results The proliferation of spleen-derived CD4+ T lymphocytes of the allergized mouse challenged by ovalbumin were inhibited by the imDCs transfected with IDO gene, the number of CD4+ T lymphocytes treated with the imDCs transfected pEGFP-N1-IDO eukaryotic expression vector decreased significantly compared with that in control group (P

Objective To survey the non-intellectual factors, motivation of nurses on the impact of nursing health education. Methods 575 cases of nurses were investigated using a serf-designed questionnaire with reference to a lot of recent related articles. Results The mean motivation score of health education was 23.82. Most nurses were still not have a systematic understanding of nursing health education from the aspect of motivation. Conclusions In order to ensure effective implementation of nursing health education, various measures and methods should be adopted to improve nurses' systematic understanding of the content and the purpose of nursing health education.

Objective　To investigate the relationship of the expressions of common β (βcR) and specific α chains of IL-5, IL-3 and GM-CSF receptors with the eosinophils（Eos） apoptosis for the roles of these receptors in asthma. Methods　All of 12 guinea pigs were equally randomized into normal and ovalbumin sensitized asthmatic group. The apoptosis of hypodense and normodense Eos in bronchoalveolar lavage fluid (BALF) was detected by TUNEL method. The expression of common β and specific α chains of the 3 receptor mRNA were measured with RT-PCR and in situ hybridization. Results　More Eos were observed in asthmatic group than in normal group, especially those in hypodense (P<0.01). The apoptotic rate of Eos in BALF were significantly lower in asthmatic group than in normal animals (P<0.01), but no difference was found between hypodense and normodense Eos. Compared with normal group, the expressions of the 3 receptor mRNA α chains were decreased in asthmatic group than in normal group (P<0.01), but βcR expression was increased significantly. More IL-3 Rα and GM-CSFRα mRNA were expressed in hypodense Eos than in normodense Eos. The contents of IL-5Rα and IL-3Rα mRNA were lower in asthmatic group than in normal group. Conclusion　The apoptosis of Eos is inhibited in BALF from guinea pigs with asthma. The expressions of IL-5, IL-3 and GM-CSF receptors in Eos are regulated through 2 mechanisms: the reduced expressions of their specific α chains which attenuating the negative regulation on Eos activation and inhibited apoptosis and the increased expressions of their βcR which enhancing the positive regulation on the 2 aspects. These data suggest that IL-5, IL-3 and GM-CSF receptors might be involved in the pathogenesis of asthma through regulating the apoptosis of Eos.

Objective To investigate the effect of dexamethasone (Dex) on the transcription of 11 ?-hydroxysteroid dehydrogenase type 1 (11?-HSD1) gene in vascular endothelial cells. The roles of p38 mitogen-activated protein kinase (p38MAPK) signaling pathway and glucocorticoid receptor (GR) were also observed. Methods Vascular endothelial cells were co-cultured with different concentrations of Dex (10-9-10-3mol/L) for 24h. Reverse transcription polymerase chain reaction (RT-PCR) was employed to detect 11?-HSD1 mRNA in each group. Normally cultured cells, without contact with Dex, served as normal control group. The cells were co-cultured with p38MAPK specific inhibitor SB20358 (10-2mol/L) and GR specific inhibitor RU486 (10-6mol/L) for 24h, then RT-PCR was employed to detect 11?-HSD1 mRNA in each group. Among the groups, Dex-treated cells and non-intervened cells were grouped as negative control and normal control, respectively. Results 11?-HSD1 mRNA/?-actin mRNA of Dex-treated groups (respectively as 0.120?0.040, 0.140?0.020, 0.280?0.030, 0.360?0.060, 0.460?0.040) were significantly higher than that of the normal control (0.030?0.004, P

Objective:To investigate the relation between expression of Bcl-2,Bax and eosinophils(Eos) apoptosis,explore effect of Bcl-2 and Bax on asthma.Methods:Guinea pigs were divided into normal and asthmatic group randomly. Ovalbumin was used to prepare model of asthma in guinea pig. Apoptosis of Eos in BALF was detected.mRNA expression of Bcl-2 and Bax in Eos were measured by hybridization and RT-PCR.Results:Comparing with normal group,Eos in BALF from asthmatic group were significantly higher; the percentage of apoptotic Eos from asthmatic group were significantly lower( P

Objective To investigate changes in glucocorticoid receptor (GR) expression and activity in lung tissue of acute lung injury induced by lipopolysaccharide (LPS) within 24h in rat. Methods A total of 70 Wistar rats were divided randomly into LPS treatment group and LPS+ Dexamethasone (Dex) treatment group. The GR mRNA and GR protein expressions in the lung tissue of LPS challenged rats were assessed by RT-PCR and Western blot at different time points. Electrophoretic mobility shift assays (EMSA) were used to determine the GR activity in the lung tissue. Results The expression level of GR mRNA was depressed, but it returned to normal level at 24h after LPS challenge. The expression level of GR was also lowered, reaching the lowest level at 8h. GR activity was decreased, reaching the lowest level at 1h, and remaining lower than that of normal control at 24h. Dex treatment showed no obvious effect on GR activity during the late period of treatment. Conclusion The GR protein expression decreases in lung tissue of acute lung injury in rats, and it maybe associated with the decreased expression of mRNA and accelerated degradation of GR protein. The activity of GR is inhibited sharply, resulting in glucocorticoid resistance.

Objective To observe the chronic hepatotoxicity of the commonly used herbicide mixture in the mice. Methods Low doses herbicide mixture diluted in mice drinking water. The parameters related to hepatic function and ultrastructural changes of the hepatocytes were examined after 18 weeks and 24 weeks of treatment. Results No changes was observed in every parameter after 18 weeks of treatment compared with the control group. After 24-week of treatment, the electron microscope revealed the ultrastructural changes in the hepatocytes, rough endoplasmic reticulum showed dilatation, mitochondria showed matrix muddy with distortion and disappearance of inner crista and the serum transaminase were higher than those in the control group(P

AIM: To explore effects of LPS,TNF-?,IL-1? on cyclooxygenase-2(COX-2) expression and prostaglandins(PGs) production in human umbilical vein endothelial cells (HUVEC). METHODS: HUVEC were stimulated with lipopolysaccharide(LPS),TNF-?,IL-1? for 24 hours. Using in situ hybridization,reverse-transcription polymerase chain reaction and immunohistochemistry, COX-2 expression in both mRNA and protein level were observed and prostaglandins in culture medium were measured. RESULTS: Resting HUVEC expressed a little COX-2 mRNA. Expression of mRNA and protein of cyclooxygenase-2 increased significantly post-stimulation with LPS,TNF-?,IL-1? and PGs raised markedly at the same time. CONCLUSIONS: Resting HUVEC expressed a little COX-2 mRNA, inflammatory stimuli induced COX-2 superexpression and PGs production. These results suggested that endothelial cells could involve in inflammation by controlling of COX-2.

AIM To investigate the effects of aminophylline (AM) on expression of Fas mRNA and apoptosis in eosinophils(Eos) of bronchalveolar lavage fluid(BALF) in asthmatic guinea pig, and the mechanism of AM promoting Eos apoptosis. METHODS After guinea pigs were stimulated by ovalbumin(OVA) 48 h, hypodense Eos(HEos) and normodense Eos(NEos) were purified from BALF by gradients of percoll. Purified Eos were incubated with different concentration of AM (10 -6 ～10 -3 mol?L -1 ) in RPMI 1640 medium 24 h, the mRNA of Fas in Eos was measured by hybridization and apoptosis was detected by TdT mediated dUTP nick end labeling. RESULT Eos cultured under the condition of AM administration in vitro, apoptotic Eos increased and the expression of Fas mRNA increased in a dose dependent manner. The expression of Fas mRNA was correlated with Eos apoptosis. CONCLUSION AM promote Eos apoptosis and expression of Fas in dose dependent manner in vitro. Fas might be one of mechanism that AM promoting apoptosis of Eos.