Objective To explore the effect of cognitive-behavioral method on healthy behavior selection of in-patients with type 2 diabetes. Methods All patients with type 2 diabetes involved in this study were divided into the experimental group(99 patients) and the control group(96 patients). The experimental group was given health education with cognitive behavioral method, and the control group received only common health education. The comparison on the knowledge about diabetes mellitus and indicators of glucose metabolism on the first and 6th months between the two groups was performed. Results The knowledge about diabetes mellitus and indicators of glucose metabolism on the first and 6th months in the experimental group were better than those in the control group. Conclusions Cognitive-behavioral method can increase the treatment compliance of in-patients with type 2 diabetes and improve the glucose metabolism indicators, and created favorable condition for prevention of diabetes complications.

Objective:On the basis of synthetic antigens on minoxidil ,we prepared its polyclonal antibody and established a ELISA method to detect minoxidil .Methods:We synthesized minoxidil artificial antigen with glutaraldehyde and identified by UV scan -ning.We had established a detection of minoxidil competition ELISA .Results: The UV scan showed that the minoxidil successfully coupled to a carrier protein.The conjugate of minoxidil-BSA was used to immunize BALB/c mice.And the produced antiserum showed high titer of 1:12 800 in the indirect ELISA.Its ELISA curve equation:y=-0.082 3 x+0.938 ( R2 =0.9811 ) , minoxidil mass concentration and the absorbance in 0.001-10 μg/ml showed a good linearity .Conclusion: This method has been successfully preparing minoxidil artificial antigen and polyclonal antibodies and established ELISA detection method for minoxidil ,which provides a basis for the practical application of minoxidil immunoassay method .

ObjectiveTo investigate the effect of scalp point penetration combined with rehabilitation training on content of endothelin (ET) and calcitonin gene-related peptide (CGRP) in plasma of patients with acute cerebral hemorrage (ACH).Methods90 ACH patients were randomly divided into the scalp point penetration combined with rehabilitation group (group A), the rehabilitation group (group B) and control group (group C) with 30 cases in each group. The radioimmunoassay was adopted to determinate the content of ET and CGRP in plasma of patients.ResultsAfter treatment, contents of ET and CGRP of all patients were decreased significantly (P<0.01), but group A and group B had a significant difference compared with the group C (P<0.01), and there was also a significant difference between the group A and group B.ConclusionThe scalp point penetration combined with rehabilitation training can regulate the content of ET and CGRP in plasma and make them under a dynamic balance.

ObjectiveTo explore the damage of blood vessels in carbon monoxide poisoning patients by hyperbaric oxygen, especially for damage of vein, to understand the factors aggravating inflammatory response, thus work out the homologous protective measures.MethodsFrom January 2006 to January 2008, 80 patients of carbon monoxide poisoning were selected as the observation group. Another 80 patients with other diseases were set as the control group. Two groups both accepted hyperbaric oxygen treatment. The time of angioplerosis was compared between the two groups, besides, pain reaction of the observation group was also observed.ResultsThe time of angioplerosis was significantly different between the two groups, pain sensibility intensified in the observation group compared with that before treatment.ConclusionsHyperbaric therapy is closely related with vascular inflammatory reaction of carbon monoxide poisoning patients. Protective measures should be established in order to prevent further damage of vessel, relieve pain of patients and ensure smooth progress of the treatment.

Objective: To evaluate the clinical efficacy of scalp penetrating technique plus rehabilitation therapy in treating acute cerebral hemorrhage. Methods: The patients were allocated randomly by computer into scalp acupuncture plus rehabilitation group (A),rehabilitation group (B) and Western medication control group (C). Results: The total effective rate and the curative and remarkable effective rate were 92.9% and 75.0%respectively in Group A, and 84.6% and 69.2% respectively in Group B and 75.9% and 37.9% respectively in Group C. There was a very significant difference in comparison of Group A and Group B with Group C (P＜0.01, P＜0.05). There was no significant difference between Group A and Group B (P＞0.05). But the total effective rate was significantly higher in Group A than in Group B. Conclusion: Scalp acupuncture plus rehabilitation therapy can obviously enhance the clinical effect in cerebral hemorrhage, reduce the neural deficit due to acute cerebral hemorrhage and improve the ability in daily life.

Objective To develop a multiplex sequence-specific PCR assay for simultaneous screening of 5 types of JAK2 mutations and investigate its clinical application value. Methods Multiplex sequence-specific PCR assay for simultaneous screening of JAK2 V617F, K539L (include 2 types of gene mutations), N542-E543del and E543-D544del mutations were developed. 115 patients with myeloproliferative diseases (MPD) including 61 polycythemia vera (PV) cases, 43 essential thrombocythemia (ET) cases and 11 primary myelofibrosis (MF) cases were analyzed. Results The assay can screen the 5 types of JAK2 mutations efficiently. The detection sensitivity is 1% for JAK2 V617F mutation and 0.1% for the other mutations. JAK2 V617F mutation and JAK2 exon12 mutation were detected in 56 and 3 of the 61 PV samples, respectively. 27 of the 43 ET samples and 6 of the 11 MF samples were JAK2 V617F positive, but no JAK2 exon12 mutation was found in both groups. The 3 cases carrying JAK2 exon12 mutation had the clinical feature of erythrocytosis and erythropoietin-independent erythroid colony formation but without apparent leukocytosis, thrombocytosis and splenectasis. Conclusion The assay can simultaneously screen 5 types of JAK2 mutations with high sensitivity and thus lead to an increased detection rate.

Objective To integrate the "volunteer service" culture into standardized training nurses and to build the awareness of participation in volunteer service of a hospital. Methods A total of 75 new nurses in 2016 and 130 new nurses in 2017 were selected as control group and observation group respectively by cluster sampling. Two groups all included "volunteer service" in pre-job training advocating active participation in volunteer service in hospital. Observation group added "integration management" in volunteer service. The situation of volunteer service between two groups were compared after training. Results After training, the mean of integration of volunteer service was (2.23±0.21) in observation group and (1.14±0.16) in control group with a significant difference (t=2.87,P< 0.01). There were 36.15% of nurses participated in daily volunteer service, 11.54% of nurses donated blood voluntarily, 18.46% of nurses participated in volunteer service of public emergency events in observation group, which were better than those in control group with significant differences (χ2=6.95,4.31,5.43;P<0.05). Conclusions The application of "integration method" in hospital volunteer service management is in favor of improving nurses' enthusiasm for participating in volunteer service as well as establishing the spirits of "dedication, affection, cooperation and advancement".

The central venous access device (CVAD) is widely used in infusion of intravenous high nutrient solution, chemotherapy drugs, rapid infusion, blood transfusion, etc. CVAD is left in the patient′s blood vessels for a long time, although it has certain advantages in intravenous therapy, but also risks associated with it. In the past 20 years, many studies have focused on the mechanism of CVAD on vascular injury, trying to explore its mechanism from a different perspective at the microscopic level of pathophysiology. This article will review the three aspects of acute damage to the blood vessel caused by CVAD catheterization and chronic changes caused by indwelling catheters, analysis of the causes of vascular injury caused by CVAD catheterization, and research on preventing vascular injury caused by CVAD catheterization, aiming at providing reference for clinical intravenous therapy.

Postpartum depression(PPD)is one of the most common types of postpartum psychiatric syndromes. Because of the complex and changeable characteristics in PPD disease and the special period after childbirth, there are many clinical limitations in the study of this disease. Therefore,the preparation and establishment of a proper animal model closed to clinical and behavioral evaluation method plays an important role in study of its pathogenesis. This review mainly introduces the commonly used postpartum depression animal models and the behavioral evaluation method. It is hoped to provide a reference for further study of PPD pathogenesis and for the drug research and development.

Objective:To explore the current situation, hotspots and trends of multi-sited license of nurses in China based on China National Knowledge Infrastructure (CNKI) , Wanfang and VIP database, so as to provide reference for the research of multi-sited license of nurses in China.Methods:CiteSpace 5.1.R6 was used to conduct a visual analysis of the research on the multi-sited license of nurses in databases from the following seven aspects, including the annual number of documents, authors, institutions, journal sources, high-frequency keywords, keyword clustering and keyword burst words. The retrieval time limit was from the establishment of the database to December 31, 2021.Results:A total of 100 articles were included. The annual number of documents issued was on the rise, but the total amount was low. Among the authors, Sun Hongyan published 11 articles, ranking first. There were 88 sending agencies, with little cooperation and communication among them. The sending agencies mainly relied on universities or their affiliated hospitals. The source of journals is mainly nursing journals. There were 125 key words in total. The cluster results showed that the multi-sited license research of nurses mainly included door-to-door service, practice registration, online appointment nurses, willingness, nursing quality and influencing factors. The results of keyword burst showed that the research on influencing factors, nursing quality, Internet +, and nursing services had continued since 2020, and might continue to become research hotspots in the next few years.Conclusions:The multi-sited license of nurses in China is still in the exploratory stage, and the research content remains at the theoretical level, lacking empirical research.