Objective:To observe the influence of electroacupuncture at Shu-points of the Five Zang-organ in domestic rabbits with acute allergy neuritis,a classical animal model,and to explore the immunoregulatory mechanism responsible for the regimen by detecting expression of the costimulatory molecules in sciatic nerve.Methods:ELISA was used to examine proteins of CD28,CTLA-4,B7-1,B7-2 in sciatic nerve of P2 immunized EAN domestic rabbits.Results:It was showed that the expression of CTLA-4 and B7-2 was obviously up-regulated(P

Objective: To research the clinical curative effect and explore the mechanism of penetrating of scalp-point on acute hypertensive intracerebral hemorrhage (ICH). Methods: 66 patients were randomly divided into two groups: one is the scalp-point therapeutic group, the other is the western medicine group. The two groups were all treated by dehydration, etc. In addition, there are 20 normal people to be set as the healthy group. To select the nervous functional deficit mark, activity of daily living (ADL) form, the amount S-100B protein as indexes and analysis the results. Results: the recordings indicate the patients' Serum S-100B protein are related with nervous functional deficiency mark. After 28 days, the recovery of patients' nervous function of scalp acupuncture group was obviously superior to that of control group (P＜0.05). Conclusions: The results suggested that the patients' Serum S-100B had positive correlation with nervous functional deficit; The penetrating of scalp-point had the better regulatory effect on the level of Serum S-100B, and could improve the nervous functional deficit and ADL, so as to improve the coordination and compensation function and the general nervous function between cortical functional areas

Acupuncture Points ; Acupuncture Therapy ; Female ; Hirschsprung Disease ; therapy ; Humans ; Infant

Objective To observe the clinical efficacy of acupuncture with points selection based on yin-yang and qi-blood in treating insomnia by using polysomnography.Method Seventy-five insomnia patients were randomized into a treatment group of 38 cases and a control group of 37 cases. The treatment group was intervened by acupuncture with points selection based on yin-yang and qi-blood, while the control group was by oral administration of Estazolam. The polysomnography was used to assess sleep condition before and after 2 treatment courses, and the clinical efficacies were compared between the two groups.Result The sleep parameters [sleep latency, rapid eye movement (REM) latency, total sleep duration, and sleep efficiency) were significantly changed after the treatment in both groups (P<0.01). The Non-REM (NREM) and REM duration of the treatment group were significantly changed after the treatment (P<0.01). The REM duration of the control group was significantly changed after the treatment (P<0.01). After the treatment, the NREM and REM durations of the treatment group were significantly different from that of the control group (P<0.01). The sleep durations in S1, S2, S3, and S4 stages during NREM were significantly changed in the treatment group after the intervention (P<0.01). The sleep duration in S1, S2 during NREM was markedly changed in the control group after the intervention (P<0.01 ,P<0.05). After the treatment, the sleep durations in S1, S2, S3, and S4 during NREM of the treatment group were significantly different from that of the control group (P<0.01). The total effective rate was 73.7% in the treatment group versus 75.7% in the control group, and the difference was statistically insignificant (P>0.05).Conclusion Acupuncture with points selection based on yin-yang and qi-blood is effective in treating insomnia, and it can improve the sleep structure and quality.

Objective To analyze the single resistance and multiple resistance situation of of Helicobacter pylori (Hp) in Qingd‐ao area to commonly used antibacterial drugs and to investigate the mutation characteristics of drug‐related resistant genes .Methods Hp was isolated from the mucosal samples of gastric antrum .The agar diffusion test was used to determine the susceptibility of Hp to clarithromycin ,levofloxacin and metronidazole .The 23s rRNA of clarithromycin resistance gene ,gyrA of levofloxacin resistance gene and rdxA of metronidazole resistance gene were amplified by using PCR ,after the PCR products sequencing ,the sequence com‐parative analysis was performed by the ClustalW2 software .Results The drug susceptibility test results found that the single re‐sistance rates of 134 strains of clinically isolated Hp to clarithromycin ,levofloxacin and metronidazole were 40 .3% ,34 .3% and 43 .3% ,respectively .Only 17 .9% of Hp strains were susceptible to these 3 kinds of antibacterial drug ;the multi‐drug resistance rate was 29 .9% ,triple drug resistance rate was 9 .0% ;furthermore ,the double resistance rate of levofloxacin plus metronidazole was significantly lower than that of clarithromycin plus levofloxacin ,the difference was statistically significant(3 .0% vs .10 .4% ,χ2 = 5 .96 ,P=0 .015) .The drug resistance genes sequence analyses showed that the commonest mutation locus was A2143G (77 . 8% ) ,the commonest mode of gyrA gene mutation was N87K(78 .3% ) ,and which of rdxA gene was nucleotide insertion into loci 20/32 ,generating the frameshift mutation with the mutation rate of 44 .8% .Conclusion The multi‐drug resistance rate of Hp is high in Qingdao area .The effective antibacterial drugs should be selected according to the drug susceptibility test results .Levofloxa‐cin could serve as the first line drug for the eradication therapy scheme in this area .

Objective:To study the influence of different methods on serum soluble intercellular adhesion molecule1 (sICAM1) in patients with acute cerebral infarction. Methods: Double antibody enzyme-linked immunoadsorbent assay was used. Results and Conclusion: Scalp point-to-point acupuncture has a significant reducing effect on abnormally elevated serum sICAM1 in patients with acute cerebral infarction. Its curative effect is superior to that of simple Western medicine. There is a significant difference between them (P＜0.05).

The authors punctured the acupoints Jianyu (LI 15), Jianliao (TE 14), Jianzhen (SI 9), Binao (LI 14), Quchi (LI 11), Shousanli (LI 10) and Hegu (LI 4) and applied massage to the affected regions to treat 32 cases of the myotenositis of long head of biceps brachii. The results showed 8 cases were cured, 10 cases were remarkably effective, 11 cases were effective and 3 cases got no effect; the total effective rate was 90.6%.

In the treatment of 48 cases of peripheral facial paralysis by puncturing Yangbai (GB 14)toward Yuyao (Ex-HN 4), Dicang (ST 4) toward Jiache (ST 6), Xiaguan (ST 7) toward Jiache (ST 6),Sibai (ST 2) toward Quanliao (SI 18), Baihui (GV 20) toward Taiyang (Ex-HN 5), Yintang (Ex-HN 3)toward Shangen (Ex-HN), Cuanzhu (BL 2) toward Jingming (BL 1), and Hegu (LI 4) toward Laogong (PC 8) on the sick side, with other acupoints added in accordance with the symptoms, the results showed cure in 39 cases, remarkable effect in 7 cases, failure in 2 cases, and the curative rate in 81.2% and the effective rate in 95.8%.

Objective: To investigate the effect of scalp point-through-point acupuncture on 200 kDa neurofilament protein (NF-200) in rats with acute cerebral infarction and explore its mechanism on nerve plasticity in cerebral infarction rats. Methods: Healthy male Wistar rats were randomly allocated to sham operation (Group A), model (Group B) and acupuncture (Group C) groups. A rat middle cerebral artery occlusion (MCAO) model of cerebral ischemia was made. NF-200 mRNA was measured by reverse transcriptase polymerase chain reaction (RT-PCR) in each group on the 7th, 14th and 28th days. Results: The cerebral expression of NF-200 in group C was significantly different from those in groups A and B (P＜0.05); there was a significant difference between groups C and B or A at different time windows (P＜0.01),indicating that scalp point-through-point acupuncture could improve the cerebral expression of NF-200. Conclusion: Scalp point-through-point acupuncture can improve neural function,promote the recovery of limb function and increase the expression of NF-200 after cerebral ischemia, exerting a regulative effect on neuronal plasticity in the brain.

The expression of the cytokines IL-2, IL-10, TNF-alpha and their roles in mice with herpes simplex viral encephalitis (HSE) were studied. By using semiquantitative reverse transcription polymerase chain reaction (RT-PCR), the expressions of IL-2, IL-10 and TNF-alpha mRNA in control group, HSE group and acyclovir (ACV)-treated group were detected and the pathological changes of brain were observed. It was found that after HSV1 infection, the cerebral lesions of haemorrhage and necrosis in mice were observed under the microscopy, and the levels of IL-2, IL-10 and TNF-alpha were increased remarkably. After treatment with ACV after HSV1 infection, the cerebral lesions in mice were improved, the level of IL-2 maintained stable, IL-10 was increased consistently, and TNF-alpha was decreased significantly as compared with those in HSE group. In acute HSE, many cytokines are upregulated, including IL-2, IL-10 and TNF-alpha to eliminate virus and TH1 type response is dominant. In convalescence, there is a shift in the cytokine expression profile from TH1 profile to TH2 profile and the shift can inhibit the overexpression of immune response in animals. ACV has remarkable effects in the treatment of HSE.