This article analyzed the basic conditions of the construction for the key subjects in Dis-ease Control and Prevention institutions (CDC), and described the status quo, development direction and goal of the key subject construction using Hunan province CDC (HNCDC) as an example. Finally, it was discussed that four important aspects of the work should be paid more attention during the key subject con-struction in HNCDC.

Objective To know the status of disinfection conducted in the hospitals at province level in Hunan province aimed to make the intervention strategy for prevention and control of diseases transmission. Methods The related items of hospitals at province level were examined and evaluated according to the national standards such as GB15982-1995 Hygienic Standard for Disinfection in Hospital, GB15981-1995 Evaluating Method and Standard for the Efficacy of Disinfection and Sterilization, GB8987-1996 Requirements for Hospital Sewage Discharge, GB18466-2001 Requirements for Medical Organization Sewage Discharge, GB15980-1995 Hygienic Standard of Disinfection for Single Use Medical Products and Technical Standard for Disinfection (2002). Results As for the disinfection effect in the present investigation, the total qualified rate was 90.10%, the annual qualified rate was increasing. Conclusion The surveillance of hospital disinfection is a long-term task, to supervise and improve the hospital disinfection is necessary to ensure patient and society safety.

ObjectiveTo investigate the clinical characteristics of nodular goiter in Changchun area. Methods An retrospective analysis was made on 4 453 cases of nodular goiter proved by pathology in a period of 39 years. Results Surgery performed for nodular goiter accounted for 44.48% of all thyroid operations, with a rate of male to female of 1∶4.95. The male patients were significantly older than female. The correct diagnosis rate of nodular goiter was 71.95%, thyroid adenoma and thyroid cyst were common misdiagnosis. The right lobe was more subject to thyroid goiter than the left one, and canceration developed in 3.66% of all nodular goiter. Conclusions Changchun is an area of iodine deficiency. There are close relations between pregnancy, lactation, menses and the development of nodular goiter. Nodular thyroid goiter is subject to canceration.goit

OBJECTIVE: To explore the clinical characteristic, the CT, MRI features, diagnosis and treatment of low grade myofibroblastic sarcoma in head and neck. METHOD: Six cases of low grade myofibroblastic sarcoma in head and neck were diagnosis and reviewed retrospectively. Routine preoperative CT and MRI examination were performed. RESULT: All cases were operated one case with radiotherapy before operation was given with a total dose of 60 Gy. The patients were follow-up for 6 months to 5 year and no recurrence was found. No complications occurred in 6 cases. CONCLUSION It has been confirmed in this research that LGMS is a low-grade malignangt tumor. It was difficult to make early diagnosis through frozen section. The final diagnosis depend on paraffin section and immunohistochemisty. Extended local excision with tumor-free margin is the treatment of choice.
Adult ; Aged ; Female ; Follow-Up Studies ; Head and Neck Neoplasms ; diagnosis ; surgery ; Humans ; Male ; Middle Aged ; Myosarcoma ; diagnosis ; surgery ; Retrospective Studies ; Young Adult

This study is to investigate the effect of osthol on osteoclasts' activity, bone resorption as well as apoptosis in vitro, and explore the mechanism of osthol in preventing osteoporosis. Osteoclasts were separated from long-limb bones of new born rabbits, cultured in 24-well plate with glass slices and bone slices, and treated by 1 x 10(-5) mol x L(-1) osthol. Osteoclasts were identified by observing live cells with phase contrast microscope, HE staining, TRAP staining and toluidine blue staining of bone resorption pits. The numbers of bone resorption pits were counted as well as the surface area of bone resorption on bone slice. Osteoclasts were stained with acridine orange to detect the cell apoptosis. The ratio of apoptotic osteoclasts was observed under fluorescence microscope. The gene expression of RANKL, OPG, TRAP and p-JNK1/2 protein expression were examined using real time PCR and Western blotting, respectively. Comparing with the control group without osthol, the rates of apoptotic osteoclasts increased obviously and the number and area of bone resorption pits decreased evidently with 1 x 10(-5) mol x L(-1) osthol. There is significant difference between control group and experiment group treated by 1 x 10(-5) mol x L(-1) osthol. Therefore, the osthol through RANK+RANKL/TRAF6/Mkk/JNK signal pathway inhibits the osteoclasts activity, enhances osteoclasts apoptotic and inhibits the bone resorption.

Objective To observe the therapeutic effects of Liuwei Shunji Capsule on survival state, visceral hypersensitivity, the content of 5-HT of model rats with liver depression and spleen deficiency-type irritable bowel syndrome (IBS), and explore its mechanism.Methods SD rats were randomly divided into normal group, model group, pinaverium bromide group and Liuwei Shunji Capsule of high, medium and low dose groups. Senna with restraint stress was used in duplicating liver depression and spleen deficiency-type IBS model. From the second day after the model was established, each drug treatment group was administered with corresponding drugs once a day for two weeks. The effects of Liuwei Shunji Capsule on survival state, visceral hypersensitivity, the content of 5-HT in serum and hypothalamus were observed.Results Compared with model group, Liuwei Shunji Capsule could raise weight growth rate of model rats with liver depression and spleen deficiency-type IBS (P<0.05), improve their diarrhea, fatigue demeanor, fur quality, irritability and other symptoms, significantly decrease their visceral hypersensitivity, the content of 5-HT in serum and hypothalamus (P<0.01).Conclusion Liuwei Shunji Capsule can effectively improve survival state and intestinal disorders of rats with liver depression and spleen deficiency-type IBS, and the mechanism of treatment could be realized through regulating 5-HT level.

Objective:To explore the effect of the virulence factor OatA during Staphylococcus aureus infection.Methods: In vitro,wild type of Staphylococcus aureus USA300,OatA gene deletion strain or OatA gene complemented strain were used to infect mice bone marrow divided macrophages (BMDMs).Subsequently BMDMs were separated and the case of cell death were detected.In vivo,mice pulmonary infection model was constructed with nasal inhalation of Staphylococcus aureus.Alveolar macrophages were isolated and the case of cell death were detected.Results: In contrast to wild type and OatA gene complemented strain,OatA gene deletion strain induced severer macrophage death both in vitro and in vivo.Conclusion: The virulence factor OatA inhibits Staphylococcus aureus infection-induced macrophage death.

Objective To study the constituent ratio of thyroid nodules in inland Changchun area and compare it with the data in the coastal Shanghai area Methods A retrospective analysis was made on 9216 cases of thyroid nodules proved by pathology in a period of 39 years in Changchun and on 7018 cases in a period of 37 years in Shanghai, to compare the difference of the constituent ratio of thyroid cyst, nodular goiter, thyroid adenoma and thyroid carcinoma in these two areas Results The constituent ratio of thyroid cyst in Changchun and Shanghai area was 1 83% and 8 68%, that of nodular goiter was 48 31% and 18 62%, that of thyroid adenoma was 39 30% and 49 94%, and that of thyroid carcinoma was 10 55% and 22 76%, respectively There was a significant difference in the constituent ratio of thyroid nodules between the two areas Conclusion The difference is significant in the constituent ratio of thyroid nodules between China inland and coastal areas

OBJECTIVETo investigate the effect of genistein on osteoblast proliferation, cellular cycle, apoptosis and differentiation of osteoblasts cultivated under hypoxia conditions.

METHODRat osteoblasts were isolated from calvarias by enzyme digestion and a hypoxic model was established by in a triple-gas incubator. Rat osteoblasts were grouped into the normoxic control group, the hypoxia control group and the hypoxia administration group which was subdivided into Ge-6 group, Ge-5 group and Ge-4 group, to which genistein was administered at doses of 1 x 10(-6), 1 x 10(-5), 1 x 10(-4) mol x L(-1). The cell survival rate, lactic dehydrogenase leakage rate, apoptosis and differentiation of osteoblasts were observed for each group at 3 h after hypoxia, and the gene expression of HIF-1alpha, Bcl-2, Caspase-3 was detected by Real time RT-PCR. Forty-eight hours after hypoxia, osteogenic differentiation markers including alkaline phosphatase activity and nodules were detected.

RESULTCompared with the hypoxia control group, the hypoxia administration group displays a significant increase in the survival rate and a decreased in LDH leakage rate, apoptosis rate and percentage of S + G2 phases. Besides, the mRNA level of HIF-1alpha and Bcl-2 were enhanced, the mRNA level of Caspase-3 was inhibited.

CONCLUSIONGenistein has an effect on protecting osteoblasts from hypoxia.

Alkaline Phosphatase ; metabolism ; Animals ; Apoptosis ; drug effects ; Calcification, Physiologic ; drug effects ; Caspase 3 ; genetics ; Cell Cycle ; drug effects ; Cell Hypoxia ; Cell Survival ; drug effects ; Cells, Cultured ; Genes, bcl-2 ; Genistein ; pharmacology ; Hypoxia-Inducible Factor 1, alpha Subunit ; genetics ; L-Lactate Dehydrogenase ; metabolism ; Osteoblasts ; cytology ; drug effects ; metabolism ; RNA, Messenger ; metabolism ; Rats ; Rats, Sprague-Dawley

Objective To explore the interaction of sleep quality and depression among patients with type 2 diabetes. Methods With multistage cluster sampling, all the participants were interviewed with self-designed questionnaire, diabetes-specific quality of life scale and self-rating depression scale, and Pittsburgh sleep quality index scale. Chi-square test was used for qualitative data. Risk factors were analyzed by means of multiple linear regression or logistic regression model. The indicator of interaction was calculated according to the delta method. Results There were 944 residents involved in the final analysis, including 365 males and 579 females. The average age was (64. 0 ± 10. 2) years. Compared with patients with type 2 diabetes mellitus( T2DM) who had good sleep quality and no depression symptoms, the risk of quality of life in those with good sleep quality but depression was 2. 75 (95% CI 1. 59-4. 77); and the risk of quality of life in those with poor sleep quality and no depression was 1. 55(95%CI 1. 03-2. 33). The risk of quality of life in those with poor sleep quality and depression was 4. 97(95% CI 2. 34-9. 63). Due to poor sleep quality and depression in patients with T2DM the combined interaction index was 2. 48 (95% CI 1. 44-4. 29), the relative excess risk was 3. 42(95% CI 2. 16-4. 67), and the attributable proportion was 0. 51(95% CI 0. 32-0. 70). Conclusion A combined interaction of poor sleep quality and depression in affecting the quality of life was found in type 2 diabetic patients. When both factors existed at the same time, the interaction effect of these 2 factors was greater than the single one.