Objective To investigate the effect of knocking down miR-19a on proliferation and apoptosis of human glioma cell line U251 in vitro.Methods U251 cells were cultured routinely.MiR-19a inhibitor was transfected into U251 cells by Lipofectamine 2000.At the same time,the negative control group and blank control group were established.The expression level of miR-19a was detected by RT-PCR.and cell proliferation was analyzed by CCK-8 assay.The changes of cell apoptosis and cycle were monitored by flow cytometry.Results Compared with the negative control group and blank control group,qRT-PCR showed that the expression level of miR-19a was significantly reduced after transfection (F=124.72,P ＜ 0.01).CCK-8 revealed that proliferation ability of miR-19a inhibitor group was significantly suppressed.The cell survival rate in miR-19a inhibitor group after 48 h was (48.27-±8.23) ％,compared with the blank control group (100.00 ％),the difference was statistically significant (t =12.45,P ＜ 0.01).After low-expression of m iR-19a,the G0/G1 phase cells were increased and S phase cells were decreased.The low-expression of miR-19a could induce cell apoptosis.Conclusions Low-expression of miR-19a can inhibit cell proliferation,block G1/S transition and induce apoptosis in human glioma cell line U251.miR-19a may serve as an attractive target of gene therapy for glioblastoma.

AIM: To observe the heart rate、 electrocardiogram and electrophysiology effect of Qibo Capsule (Radix astragali, Radix aconii lateralis praeparata, etc) on rabbit model with sick sinus syndrome (SND), and to explore their therapy and mechanism. METHODS: 30 rabbits were used to establish sick sinus syndrome models and divided randomly into 3 groups: model group、 Qibo Capsule group、 Xinbao Pill group. To observe the changes of heart rate、 electrocardiogram、electrophysiology after 10 d. RESULTS: Comparison with the model group, the Qibo Capsule group had significant statistics differences in SCL (sinus cardiac cycle)、 SACT (sinoartrial conduction time)、 SNRT (sinus node recovery time)、 SNRTc (corrected sinus node recover time) and HR (heart rate) item (P0.05). CONCLUSION : The experiments show that Qibo Capsule is effective in the recovery of the functional harm of SND rabbits. It is worth further studys.

Objective To investigate the effect of thrombin and hemoglobin on aquaporin (AQP) and the correlation between AQP and hydrocephalus.Methods Eighty-four clean grade healthy male SD rats were randomly divided into 3 groups:a control group,a thrombin group,and a hemoglobin group using the random number table method.A hydrocephalus model was induced by injecting isotonic saline (0.3 ml),thrombin (0.3 ml[10,U/ml]) and hemoglobin (0.3 ml[150 mg/ml]),respectively into the cisterna magna.According to the deficiency and complement way,each group maintained 24 rats.The relative area of the lateral ventricles,the expression of AQP1 and AQP4,and the correlation between AQP and the area of the lateral ventricles were observed at 1,3,7,and 14 d after molding.Results (1) Compared with the control group,both the thrombin group and hemoglobin group showed hydrocephalus at 1 ,3 ,7 and 14 d,and they were most obvious at 1 day (6.94±0.19% and 6.58±0.15% vs.3.40±0.13%,6.06±0.12% and 5.79±0.09% vs.3.55±0.15%,5.80±0.13% and 5.58±0.08% vs.3.78±0.18%,5.66±0.14% and 5.47±0.13% vs.3.52±0.18 %,respectively).There were significant differences (all P<0.01).(2) The increase of AQP1 was mainly in the basal membrane and apical membrane of ventricular choroid plexus epithelial cells,and the increase of AQP4 was mainly in the ependymal cell of ventricle.The relative expression levels of AQP1 and AQP4 at 1,3,7,and 14 d in the control group were 1.09±0.07 and 1.30±0.15,0.91±0.06 and 1.18±0.12,1.33±0.17 and 1.16±0.08,1.22±0.11 and 1.00±0.10,respectively;the thrombin group were 4.40±0.14 and 3.69±0.11,3.88±0.11 and 3.17±0.07,3.55±0.07 and 2.86±0.13,and 3.36±0.07 and 2.70±0.07,respectively,the hemoglobin group were 4.24±0.07 and 3.55±0.10,3.77±0.08 and 3.04±0.09,3.46±0.07 and 2.76±0.08,and 3.31±0.10 and 2.62±0.08,respectively;the relative expression levels of AQP1 and AQP4 of the thrombin group and hemoglobin group at each time point were significantly higher than those of the control group.There were significant differences among the groups (all P<0.01).There were no significant differences in the relative expression levels of AQP1 and AQP4 mRNAs in the hemoglobin group at each time point (P>0.05);in the thrombin group and hemoglobin group,compared with those at 1 d,the expression levels of AQP1 and AQP4 at 3,7,and 14 d were significantly decreased (all P<0.01);compared with those at 3 d,AQP1 was decreased significantly at 7 and 14 d (P<0.05).The differences were statistically significant (P<0.05).(3) The relative expression levels of AQP1 (r=0.983,P<0.01) and AQP4 (r=0.987,P<0.01) in the thrombin group at each time point were positively correlated with the contralateral ventricular area;and the relative expression levels of AQP1 (r=0.964,P<0.01) and AQP4 (r=0.962,P<0.01) in the hemoglobin group at each time point were positively correlated with the contralateral ventricular area Conclusions After injecting thrombin and hemoglobin into subarachnoid space,it could cause the increased expression levels of AQP1 and AQP4 of ventricles and their surrounding areas.Thrombin and hemoglobin may be the important mediating factors of hydrocephalus after subarachnoid hemorrhage.

Objective To compare the difference of ultrasound and digital subtraction anginography (DSA) in diagnosing carotid atheriosclerosis. Methods A total of 340 patients of ischemic cerebrovascular disease with carotid atheriosclerosis were examined with ultrasound and DSA. They were divided into cerebral infarction group (n=235) and transient ischemic attack (TIA, n=105), and also were divided into the young-aged group (n=54), middle-aged group (n=137) and old-aged group (n=149) by age. Results Both of the ultrasound and DSA found that the incidence of carotid artery stenosis in cerebral infarction was higher than that in TIA (P<0.05);the incidence that of carotid artery stenosis in old-aged group were higher than that in middle-aged and young-aged group (P<0.05), and in middle-aged group was higher than that in young-aged group. Taking DSA as standard, the accordance rate of ultrasound in diagnosing stenosis, clot and both of them were 89.39%, 80.85%, 87.15%, respectively. Conclusion Carotid artery ultrasound has high sensitivity in diagnosis of carotid atheriosclerosis. Either carotid artery ultrasound or DSA has advantages. With combination of two methods, there will be an important significance in the diagnosis, clinical treatment, assess before operation and follow-up after operation in patients with carotid atheriosclerosis.

Objective To summarize the echocardiographic findings and clinical characteristics of Kawasaki disease(KD) complicated with coronary artery thrombosis (CAT).Methods Thirty-five patients with KD combined with CAT were enrolled,who were admitted to Beijing Children's Hospital,Capital Medical University between July 2005 and August 2016.The clinical characteristics and echocardiographic findings during follow-ups were retrospectively studied.According to whether the childrenhad been complicated with myocardial ischemia,the patients were divided into 2 groups:ischemic group and non-ischemic group.The duration of fever,the time when the intravenous immunoglobulin(IVIG) was first injected,the time when coronary artery aneurysms (CAA) was formed,the maximum diameter of CAA and inflammatory index inthe acute phase were compared between 2 groups.Results All of the 35 children diagnosed as KD combined with CAT suffered firom CAA,and the coronary thrombosis was detected in all the cases with aneurysms.Thirty-five patients had 99 branches of CAA,of which the maximum diameter of CAA was (9.6 ± 3.1) mm(4.0-19.0 mm).Fifty-four plots of CAT were detected in the aneurysms.The diameter of CAA that thrombosis located was larger than that of which the thrombosis was not located[(10.9 ± 2.8) mm vs.(7.9 ± 2.6) mm],and the difference was significant(P ＜0.01).During 4 months to 10 years and 8 months [(39.2 ±29.5) months] follow-ups,CAA regressed in 32 branches [32.3％ (32/99 branches)],of which 4 branches [4.0％ (4/99 branches)] completely regressed to the normal diameter.The maximum diameter of CAA regressed was smaller than the maximum diameter of CAA consistence [(7.3 ± 1.9) mm vs.(10.6 ± 3.0) mm],and the difference was significant (P ＜ 0.01).Out of 35 patients,15 cases [42.9％ (15/35 cases) had myocardial ischemia,while the other 20 cases[57.2％ (20/35 cases)] didn't have.Among 15 cases with myocardial ischemia,6 cases[17.1％ (6/35 cases)] had myocardial infarction,4 cases [11.4％ (4/35 cases)] had heart failure,and 1 case[2.9％ (1/35 cases)] died of acute heart failure complicated with severe ventricular arrhythmia.Compared with non-ischemic group,the children in the ischemic group had longer duration of fever[(19.1 ± 7.8) d vs.(12.1 ± 3.3) d],higher white blood cell account in the acute phase[(24.8 ± 13.5) × 1012/L vs.(19.7 ±4.0) × 1012/L],later treatment of IVIG [(13.9 ± 5.5) d vs.(9.8 ±3.8) d],and earlier CAA formation [(16.0 ±4.9) d vs.(20.9 ± 14.5) d],and the differences were statistically significant (all P ＜ 0.05).Conclusions CAT of children with KD commonly originates from CAA.Patients who have more serious inflammatory reaction in the acute phase,earlier formation,heavy severity and longer consistence of CAA are prone to have myocardial ischemia.Echocardiographic study plays an important role in monitoring CAA,detecting the CAT and finding the early left ventricle dysfunction,which is of clinical significance.