Aim To discuss whether specific Egg yolk antibody(IgY) can be used for snake venom antigens detection.Methods Chickens(white Leghorn) were immunized with detoxicated king cobra venom by formaldehyde.Egg yolk antibody were isolated from egg yolk,and labeled with horse radish peroxidase(HRP).Snake venom antigens samples,including king cobra venom,cobra venom,bungrarus fasciatus venom,bungrarus multicinctus venom,agkistrodon actus venom and Guangdong viper venom,were detected using ELISA,and sensitivity,precision and specificity were tested,respectively.Results At about 32 ?g?L~(-1),the king cobra antigens were detected using the method;Linear relation was better(r=0.963) when the concentration of kingcobra venom was within 32～750?g?L~(-1).The method had good specificity and no cross reactivity was observed among the reagents and agkistrodon acutus guenther venom and vipera russelli siamensis smith venom;little cross reactivity was shown with bungarus multicinctus blyth venom and bungarus fasciatus chmeider venom;cross reactivity was obvious with cobra venom;the average intra-assay coefficient of variation(CV) was 1%～3%,and the inter-assay CV was within 8%.Conclusions The study indicates that IgY can be good reagents for snake venom antigens detecton,and the study provides foundation for the development of the diagnosis kits of snakebites.

Objective To explore the relationship of serum tumor necrosis factor receptor 1 (TNFR1),and TNFR2 with early diabetic nephropathy in type 2 diabetes patients.Methods A total of 70 patients with type 2 diabetes and 35 healthy person were selected from December 2013 to November 2014 in the Fifth Hospital of Datong,the urinary albumin excretion rate (UAER) was determined,and were divided into three groups:normal control group (n =35),simple diabetes group (n =35),and early diabetic nephropathy group (n =35).Enzyme-linked immuno sorbent assay (ELISA) method was used to determine TNFR1 and TNFR2.Results Compared to normal control group [TNFR1 (302.5 ± 60.0) pg/ml,TNFR2 (62.1 ±9.8)pg/ml],simple diabetes group [TNFR1 (515.1 ±78.1)pg/ml,TNFR2 (178.5 ±38.9)pg/ml],and early diabetic nephropathy group [TNFR1 (1 066.8 ± 205.6) pg/ml,TNFR2 (279.6 ± 37.1)pg/ml] were all increased;and early diabetic nephropathy group was higher than simple diabetes group (P＜ 0.05).The levels of TNFR1 and TNFR2 were positively correlated with age,course,glycated hemoglobin (HbA1c) levels(P ＜0.01),and were negatively correlated with high density lipoprotein cholesterol (HDL-C) and estimated glomerular filtration rate (eGFR) (P ＜0.01).The multiple stepwise regression analysis in eGFR had significant effects on TNFR 1 and TNFR 2 levels (P ＜ 0.01).Conclusions The levels of TNFR1,and TNFR2 in early diabetic nephropathy were increased.TNFR1 and TNFR2 may become new markers to evaluate early diabetic nephropathy.

Objective:To evaluate the clinical efficacy and safety of Danhong injections in the adjuvant therapy for angina pecto-ris. Methods:According to a random number table,totally 120 cases of patients with angina pectoris were randomly divided into the observation group and the control group. The control group was treated with the conventional therapy,such as oxygen uptake,anti-in-flammation,expectoration and relieving asthma,and the observation group was treated with Danhong injections(30ml added in 250ml glucose infusions,ivd,qd)additionally. The treatment course was 2 weeks. The hemorheology and blood lipid changes before and af-ter the treatment were recorded,the ECG was analyzed and the clinical symptom improvement was evaluated in the two groups. Re-sults:After the treatment,the hemorheological indices of the two groups were significantly reduced(P<0. 01),and the improvement of the observation group was much better than that of the control group(P<0. 01). After the treatment,the TC,TG and LDL were significantly lower and the HDL was notably higher than those before the treatment in the two groups(P<0. 01),and the improvement of the observation group was significantly better than that in the control group(P<0. 01). The total efficiency of ECG and clinical symptom improvement in the observation group was significantly higher than that in the control group(P<0. 05). Conclusion:Dan-hong injections in the treatment of angina pectoris can significantly improve the hemorheology and blood lipid to enhance the clinical ef-ficacy,which are worthy of promoted application.

Objective To investigate the expression and clinical significance of multidrug resistance gene(MDR1),multidrug-resistance-associated protein (MRP),lung resistance protein (LRP),glutathione S-transferase-?(GST-?)mRNA in non-small cell lung cancer. Methods From Oct.1996 to Oct.2001,RT-PCR was used to investigate the mRNA expression of the above four genes.We followed up the survival time one by one and calculated the probability of survival. Results The total positive rate of the four multidrug resistance gene was 53.1%,65.3%,67.3%,83.3%.There was a significant difference between the coexpression and the single positive rate( P 0.05).With the increasing expression of the MDR the curves moved to left,the survival time and probability reduced. Conclusion The coexpression of the MRG results in the MDR and affects the prognosis directly.It can be used as a criterion to evaluate the prognosis.

Objective To explore the clinical characteristics,diagnosis,therapy and prognostic features of granulocytic sarcoma (GS).Methods Retrospective analysis was used to analyse 10 cases who were diagnosed with GS.Results Five patients were diagnosed with primary GS.GS was accompanied by APL in one case,CML in 3 cases and MDS in one case.Patients were treated with resection and chemotherapy,or imatinib treatment.Follow-up data were available from 9 patients,with 2 patients being still alive.The survival of one patient who received high-dose cytarabine chemotherapy was 66 months.The other 7patients died of tumor-related diseases,and their survival ranged from 4 to 17 months.Conclusion GS is a rarely diagnosed disease.A correct diagnosis of GS depends on detailed morphological examination and immunohistochemical study.The clinical outcome of patients is poor,and AML-type chemotherapy is the proper treatment for GS.High-intensity chemotherapy and hematopoietic stem cell transplantation might improve long-term survival.

Objective: To prevent the error of overcharging fees, missing fees and mistaken identity fees, check the inpatient expense rationality with using the self-developed software system. Methods:With extracting data from HIS and other systems, check and audit the inpatient expense, such as billing classification, red account records, logic checks, integrity checks, etc, according to pre-established rules. Results:Using the auditing software can greatly improve the work efficiency and the accuracy of inpatient expense, reduce the incidence of billing dispute issue. Conclusion:This system has valuable reference and be worth generalizing because it can bring the social benefit to hospital.

Objective To investigate the performances and characteristics of femoro-acetabular impingement (FAI)on MSCT. Methods Hips jointed MSCT scans of 109 cases (as case group)and the other 120 cases (as control group)were analyzed retrospec-tively,to analyze the anatomy and osseous abnomality of FAI.Alpha angle,offset,CE angle were measured.The data were tested by using t test and ANOVA test.Results There were 57 cases of osseous protuberance at the femoral head and neck joint,26 cases of the offset reduction,17 cases of over-coverage acetabulum,41 cases of over-deep acetabulum,20 cases of acetabular retroversion,57 cases of subcortical cystic degeneration at the femoral head and neck joint and 22 cases of subchondral cystic degeneration at the ace-tabulum margin.Significant statistical differences were found between case group and control group in alpha angle value,offset and CE angle (P<0.05).Multiple comparisons demonstrated that except results between the value of alpha angle of Cam-type and Mixed-type(P=0.071),Pincer-type and the control group(P=0.083)with no statistical significance,the differences between any other two groups reached statistical significance (P<0.05).Multiple comparisons demonstrated that except result between the value of offset of Cam-type and Mixed-type(P=0.561)with no statistical significance,the differences between any other two groups reached statistical significance (P<0.05).Multiple comparisons demonstrate that except results between the value of CE angle Pin-cer-type and Mixed-type (P=0.444),Cam-type and the control group (P=0.236)with no statistical significance,the differences be-tween any other two groups reached statistical significance (P<0.05).Conclusion Different type of FAI has its unique anatomical features.Alpha angle,offsetand CE angle for the diagnosis of FAI have important reference values.

Objective To compare success rate of intubation and safety of two types of video laryngoscopes during anesthesia in uvulopalatopharyngoplasty surgery (UPPP) for obstructive sleep apnea syndrome (OSAS) patients. Methods UPPP surgery were operated to 60 patients between January and October of 2013 and those patients were randomly divided into McGrath MAC video laryngoscope group (M group), GlideScope video laryngoscope (G group), and SHUCMAN direct la-ryngoscopy (S group), with 20 patients per group. Mallampati classification scores, Cormack-Lehane grade, intubation suc-cess rate, pre-intubation vs post-intubation heart rate and blood pressure changes were recorded and compared. Results Mallampati classification scores were not significantly different between these three groups, and Cormack-Lehane grade be-tween M group and G group were also not statistically different. M and G group had distinct advantages in Cormack-Lehane grade, success rate in intubation, heart rate, blood pressure at completion of intubation (T3) and 1 minute after intubation (T4), and the differences are statistically significant (P < 0.05). Blood pressure changes were stabler in G group than M group. Conclusion The two video laryngoscopes used in anesthesia intubation during UPPP surgery can both effectively re-veal the structure of the throat, but also work with high success rate and safety. What’s more, in this study the McGrath MAC video laryngoscope was shown to be superior to GlideScope video laryngoscope.

OBJECTIVE:To observe the efficacy and safety of amiodarone combined with valsartan in the treatment of paroxys-mal atrial fibrillation in elderly patients. METHODS:130 elderly patients with paroxysmal atrial fibrillation were randomly divided into observation and control group. Control group was orally given Amiodarone tablet 0.6 g in the first week,3 times a day,0.4 g in the second week,twice a day,0.2 g in the third week,twice a day;observation group was additionally given valsartan capsule 0.4 g in the first 1-3 day(s),once a day,the dose was increased to 0.8 g if patients showed no hypotension,once a day,and then decreased to 0.4 g if the pressure was lower than 100/60 mm Hg,once a day. The treatment course for both groups was 12 weeks. Clinical efficacy,and left atrium(LA),left ventricular end-diastolic volume(LVEDV),left ventricular systolic volume(LVESV), left ventricular ejection fraction (LVEF),heart rate,cardiothoracic ratio,systolic blood pressure,diastolic blood pressure before and after treatment,and incidence of adverse reactions in 2 groups were observed. RESULTS:Total effective rate in observation group was significantly higher than control group,the difference was statistically significant(P<0.05). After treatment,LA, LVEDV and LVESV,heart rate,cardiothoracic ratio,systolic blood pressure,diastolic blood pressure in 2 groups were significant-ly lower than before,and observation group was lower than control group,LVEF was significantly higher than before,and observa-tion group was higher than control group,the differences were statistically significant(P<0.05). There was no significant differ-ence in the incidence of adverse reactions(P>0.05). CONCLUSIONS:The efficacy of amiodarone combined with valsartan is more obvious than only amiodarone in the treatment of paroxysmal atrial fibrillation in elderly patients,with similar safety.

AIM:To investigate the effect of caveolin-1 and phosphorylation of ERK1/2 on 17?-estradiol (E2) induced inhibition of vascular smooth muscle cells (VSMCs). METHODS:The proliferation in cultured VSMCs was determined by using [3H]-thymidine incorporation. The expressions of caveolin-1,MKP-1 and ERK1/2 phosphorylation were measured by Western blotting. The expression of caveolin-1 mRNA was measured by RT-PCR. RESULTS:Exposed to fetal calf serum (FCS) for 24 h,the increase in proliferation of VSMCs was detected by [3H]-thymidine incorporation. Pretreatment with various concentrations of E2 for 24 h inhibited VSMC proliferation induced by FCS. The results of Western blotting and RT-PCR showed that pretreated with 17?-estradiol for 24 h reserved the decrease in caveolin-1 induced by FCS. Western blotting results further proved that the expression of MKP-1 was significantly increased and the expression of ERK1/2 phosphorylation was decreased after incubated with 17?-estradiol. CONCLUSION:17?-estradiol increases caveolin-1 and MKP-1 expressions,and decreases ERK1/2 phosphorylation,leading to the inhibition of VSMC proliferation.