1.An outbreak of extended-spectrum beta-lactamase-producing Klebsiella oxytoca in a surgical ward
Yan CUI ; Dingxia SHEN ; Guimin ZHOU
Chinese Journal of Laboratory Medicine 2001;0(05):-
Objective To study 8 isolates of multiple antibiotic-resistant Klebsiella oxytoca in 8 different patients from one surgical ward. Methods Susceptibility test, extended-spectrum ?-lactamases (ESBLs) screening methods and some molecular biological methods were performed. Results 8 isolates of Klebsiella oxytoca were verified as closely related homologous CTX-M-3-producing strains. Conclusions The little outbreak was caused by 8 closely related homologous strains in one ward.
2.Clinical observation of radiotherapy combined with temozolomide in non-small cell lung cancer patients with brain metastases
Fei TENG ; Guimin CUI ; Hongyun SHI ; Miaoling LIU ; Yanhong LI
Journal of International Oncology 2017;44(4):271-273
Objective To investigate the recent curative effect and adverse reactions of radiotherapy combined with temozolomide in non-small cell lung cancer (NSCLC) patients with brain metastases.MethodsThe clinical date of 51 NSCLC patients with brain metastases were retrospective analyzed in Department of Radiation Oncology of Affiliated Hospital of Hebei University.Patients were divided into experimental group (n=26) and control group (n=25) according to the different treatment methods.The experimental group underwent whole brain and local tumor radiotherapy plus temozolomide.The control group only received whole brain and local tumor radiotherapy.The recent curative effect and adverse reactions of the two groups were analyzed.Results The Karnofsky performance status score of patients in the experimental group was obviously improved than that in the control group (76.2±6.4 vs.72.8±5.3), with a significant difference (t=2.06, P=0.04).The total effective rate in the experimental group was higher than that in the control group (80.8% vs.64.0%), but there was no statistically significant difference (χ2=1.80, P=0.18).Compared with the control group, the incidences of nausea and vomiting (80.8% vs.28.0%) and bone marrow suppression (84.6% vs.24.0%) in the experimental group were significantly higher, with significant differences (χ2=14.33, P=0.00;χ2=18.91, P=0.00).There were similar incidences of headache (69.2% vs.60.1%), liver and kidney damage (73.1% vs.64.0%) in the two groups, with no significant differences (χ2=0.47, P=0.49;χ2=0.47, P=0.49).Conclusion Radiotherapy combined with temozolomide can improve the quality of life in NSCLC patients with brain metastases, which has controllable and tolerable adverse reactions.
3.Influence of 4-aminopyridine on human ovarian luteinized granulosa cell proliferation, production, and apoptosis through inhibiting voltage-gated K+ channel
Zhiming ZHAO ; Na CUI ; Suxin XU ; Fulu GAO ; Guimin HAO ; Jinfeng CAO
Chinese Journal of Obstetrics and Gynecology 2008;43(12):918-922
Objective To study the influence of 4-aminopyridine(4-AP)on proliferation,production,and apoptosis through inhibiting voltage-gated K+channel(Kv)in ovarian luteinized granulosa cells.nethods Ovarian luteinized granulosa cells were recovered from 25 women with regular menses who underwent in vitro fertilization programme.The cultured granulosa cells were divided into 4 groups:blank group,4-AP treated group,human chorionic gonadotropin(hCG)-induced group and hCG+4-AP cotreated group.The final concentrations of hCG and 4-AP were 1250 U/L and 5 nmol/L respectively.The progesterone production WaS detected by the chemoluminescence method.The expression of Kv mRNA on human ovarian luteinized granulosa cell was detected by RT.PCR The influence on the early apoptosis of gTanulosa cells bv 4-AP was observed by flow eytometry.Cellular caSpage-3 activities were observed with colorimetric method and the inhibition of the cell proliferation was studied using methyl thiazolyl tetrazolium(MTT)method.Results(1)Kv mRNA wag expressed in granulosa cell.(2)The progesterone production64),(206±32),(1991±172)and(763±79)nmol/L,respectively after24 hours culture.Exposure of the(3)The flow cytometry analysis and the cellular caapase-3 A405 showed that 4-AP increased the percentage ofearly phase apoptosis(P<0.01):4-AP treated group VS blank group[(40±5)%and 0.049 ±0.009]VS[(17±4)% and 0.029±0.008],hCG+4-AP CO-treated group VS hCG-induced group[(25±4)%and0.039 ±0.0081 VS[(15±3)%and 0.022 ±0.007].(4)24 hours after treated with 4-AP and hCG,theinhibitory rate of cultured granulosa cells of 4-AP treated group was higher than the blank group(19.7%VS0).and that of hCG+4-AP co-treated group was obviously higher than hCG-induced group(34.6% VS O,P<0.01).Conclusions The voltage-gated K+ channels expressed by ovarian luteinized granulosa cellplay an important role in cell proliferation,production,and apoptosis.4-AP may inhibit differentiation ofprogesterone in granulosa ceHs through the inhibition of proliferation and induction of apoptosis.
4.Experimental study of disinfection effect of a new low-temperature plasma disinfector
Caiqin MA ; Guimin XU ; Xiangni WANG ; Yixin CUI ; Na LIU ; Xingmin SHI ; Guanjun ZHANG
Journal of Xi'an Jiaotong University(Medical Sciences) 2021;42(5):774-779
【Objective】 To observe the inactivation effect of a new low-temperature plasma air disinfector on microorganisms in the natural environment and artificial contaminated environment so as to further determine the minimum effective processing parameters. 【Methods】 First, in 0.5 m3 of super clean workbench and 1.05 m3 airtight organic glass cavity in the body, the new low-temperature plasma air disinfector developed was used to inactivate the natural bacteria in the air under different parameters. Then in 1.05 m3 airtight organic glass cavity in the body, the new low-temperature plasma air disinfector was used to treat the Staphylococcus aureus and Escherichia coli under the condition of artificial bacteria spraying. Last, the plate counting method was used to evaluate the inactivation effect of the disinfector on natural bacteria and artificial bacteria in the air. 【Results】 For the low-temperature plasma air disinfector in 0.5 m3 and 1.05 m3 confined space, the lowest effective treatment parameters for inactivated natural bacteria were 13 kV, 5 min or 12 kV, 10 min. For the low-temperature plasma air disinfection machine in 13 kV discharge voltage treatment of different time, compared with before treatment, the killing rate of Escherichia coli and Staphylococcus aureus increased significantly after treatment for 20 min (P<0.000 1). Both could meet the requirements of disinfection specifications [100%, (99.98±0.01)%≥99.90%]. 【Conclusion】 The inactivation of natural bacteria and artificial bacteria by the new low-temperature plasma air disinfector has reached the qualified standard of disinfection stipulated in Technical Specifications for Disinfection.
5. Effect of anatomical changes of frontal recess and frontal sinus on airflow after nasalisation by image reconstruction and numerical simulation
Cheng LI ; Bing ZHOU ; Jing QU ; Xiuzhen SUN ; Qian HUANG ; Guimin ZHANG ; Mingjie WANG ; Shunjiu CUI
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2019;54(11):805-812
Objective:
To describe the influence of post-operative anatomical structure changes on nasal airflow characteristics by 3D reconstruction and numerical simulation in real cases after nasalisation with Draf Ⅲ so as to explore the correlation between the changes of anatomical structure and subjective symptoms as well as airflow characteristics.
Methods:
Ten patients underwent nasalization with Draf Ⅲ in Department of Rhinology in Beijing Tongren Hospital from 2006 to 2018 were selected retrospectively. Postoperative follow-up of all patients was more than 1 year. All patients had no abnormalities in their paranasal sinus CT scans and Lund-Kennedy scores were 0 except scar. VAS scores including nasal obstruction, stimulation in frontal sinus, and headache were collected at the same period. The control model was a normal person. Numerical simulation was used for calculating airflow characteristics in deep inspiratory period of both models. Independent sample Mann-Whitney