Objective To investigate the effect of Doctor-patient Interaction Model on hemiplegia after acute stroke.Methods 200 cases with mderate to severe hemiplegia were divided into treatment group and the control group with each 100 cases according to whether their family supporting rehabilitation.Cases in treatment group received interaction model rehabilitation.They were assessed with National Institutes of Health Stroke Scale(NIHSS),Fugl-Meyer Assessment(FMA),Barthel Index(BI)10 d,1 month,3 months,6 months,12 months after rehabilitation,and the Geriatric Depression Scale(GDS)1 year after rehabilitation.Results The scores in all the assessment of the cases in the treatment group improve mostly 3 month after rehabilitation(P<0.01),while the BI improved 6-12 month after rehabilitation(P<0.01).The incidence of depression reduced 18% compared with that of the control group(P<0.01).Conclusion Doctor-patient interaction can improve the outcome of patients with moderate to severe hemiplegia.

It is becoming a new trend in wound care field to do quantitative evaluation for the effect of wounds.The pressure ulcer scale for healing (PUSH) made by national pressure ulcer advisory panel (NPUAP) was used to evaluate the effect of pressure ulcers quantitatively at first and then it was used to different classification wounds.This article reviews the advancement of validity and reliability of PUSH in inter-professional research and clinical application in order to provide reference data for correctly using the tool.

The LHX3 and LHX4 LIM-homeodomain transcription factors play essential roles in pituitary gland.Nervous system developments play certain roles in some cancers,leukemia,and deafness disease.Mutations in the genes encoding these regulatory proteins are closely associated with combined hormone deficiency diseases in human and animal models,and related to nervous system defect,tumors,leukemia and deafness.Analysis of these diseases and the biochemical properties of the LHX3 and LHX4 protein will facilitate a better understanding of the pathogenesis of these diseases.LHX3 and LHX4 are reviewed in this paper.

OBJECTIVE To investigate the knowledge about hand hygiene among the healthcare workers in the infectious disease hospital and improve their hand hygiene compliance.METHODS A standardized questionnaire was sent to 364 healthcare workers in the infectious disease hospital.The following data investigated anonymously were collected.RESULTS All the healthcare workers' answers to the choice questions were right(100.0%).However,there were significant differences in the answers to the memorized questions among the healthcare workers with different careers.The correct answers among the nurses were the highest(97.7% and 94.8%,respectively);among the doctors were 71.4% and 34.7%;and among the medical technicians were 21.5%,and 8.6%,respectively.But there were no significant differences between sexes,education degrees and service length in the three groups.CONCLUSIONS The healthcare workers of our hospital are well in the field of basic knowledge about hand hygiene.However,there are differences in the answers to the memorized questions among healthcare workers with different careers.It need to enhance the systems of training and examination for workers,especially for the doctors,technicians and all the new staff.Alcohol-based hand rub(AHR),which is prone to practice,would replace hand-washing.And it should be more supervised.

Objective To study the relationship between the serum paraoxonase-1(PON1) activity, PON1Q/R192 polymorphism and atherosclerotic thrombotic cerebral infarction(ATCI) in Chinese Han people.Methods In 48 cases with ATCI and 55 normal controls (NC group), serum PON1 activity was assayed by infrared spectrophotometer;the PON1Q/R192 polymorphism was determined by polymerase chain reaction and restriction fragment length polymorphism.Results Serum PON1 acuivity in the ATCI group was significantly lower than that in NC group[(74.41?18.85) U/ml,(113.65?26.64) U/ml, P

One-stop service is a kind of simple,highly efficient service with high quality.It embodies the 'patient-based' medical service pattern.This paper introduces the measures,experiences and benefits resulting from the application of one-stop service in the physical examination center in the outpatient department of our hospital.This service is a breakthrough in medical service and managing concepts guided by the market economy.We believe that the medical service should comply with the patient-based concept,and only by personalized and multi-facet service can the hospital make itself competitive.

Objective To investigate the relationship of nerve growth factor (NGF) between serum and follicular fluid in patients with polycystic ovary syndrome (PCOS), and its effect on pregnancy outcomes. Methods From December 2011 to November 2012, 65 PCOS patients suffered with in vitro fertilization-embryo transfer (IVF-ET) were included in PCOS group and 31 non-PCOS patients with IVF-ET were used as control group. The expressions of NGF in serum and follicular fluid were detected by ELISA on the injection day of chorionic gonadotropin (HCG). The expression levels of NGF in serum and follicular fluid were compared between PCOS group and control group. Results The NGF levels in serum[(14.38±0.42) ng/L] and follicular fluid[(9.61±0.49)ng/L] were significantly higher in PCOS group than those of control group[(11.39±0.38) ng/L and (7.55 ± 0.40)ng/L]. In PCOS group, NGF levels in serum [(14.22 ± 0.35)ng/L] and follicular fluid [(9.30 ± 0.31)ng/L] were significantly lower in pregnant group than those of non-pregnant group[(14.51±0.43)ng/L, (9.86±0.46)ng/L, P<0.01]. Conclusion The increased levels of NGF in serum and follicular fluid of PCOS patients contribute to the pathogenesis of PCOS. The high expression of NGF in serum and follicular fluid may be harmful to pregnancy in PCOS patients.

ObjectiveTo discuss the clinical significance of han nationality and kazak hepatitis B virus large surface protein detection.MethodsEnzyme linked immunosorbent assay(ELISA) method was used to examine the HBV-LP、HBV markers and quantitative real-time PCR methods were used to detect the HBV DNA in 270 patients with Hepatitis B.ResultsAmong the 270 cases,there was no significant difference between the levels of HBV DNA and HBV-LP( P ＞ 0.05 )in HBe Ag-positive patients,which was not affected by nationality.Significant difference of positive rate was observed between HBV-LP and HBV DNA( P ＜0.05) in HBe Ag-negative ones,which was not affected by nationality.HBV-Lp expression was significantly correlated with the logarithm of HBV DNA level ( r =0.986,P ＜ 0.05).ConclusionThere was higher coincidence rate between the levels of HBV-LP and HBV DNA in HBeAg--positive patients.The positive rate of HBV-LP was higher than that of HBV DNA in HBe Ag-negative patients.HBV-LP could serve as a reliable marker in the reflection of HBV the replication at protein level,and it was valuable to monitor HBV replication and prognosis of the disease,especially in HBe Ag-negative HBV infected patients.

ObjectiveTo study the expression of growth factor receptor binding protein 2(GRB2) in bladder transitional cell carcinoma (BTCC),and clarify the role of GRB2 in the development and progression of BTCC.MethodsThe GRB2 expression of BTCC tissue from 60 patients who first got BTCC (BTCC group) and adjacent normal bladder from 30 patients(control group) were detected by SP immunohistochemical method.ResultsThe GRB2 positive expression rate of BTCC group [81.7％(49/60)] was significantly higher than that of control group [30.0％(9/30)] ( X2 =12.122,P ＜0.05).According to tumor histological grade,the GRB2 positive expression rate was grade Ⅰ[66.7％( 16/24)] ＜gradeⅡ [89.3％(17/19)] ＜grade Ⅲ [94.1％(16/17)],grade Ⅰ and grade Ⅱ,Ⅲ had significant difference (P ＜0.05),but grade Ⅱ and grade Ⅲ had no significant difference(P ＞0.05).The GRB2 positive expression rate in the clinical stage was stage T1 [63.0％ (17/27)]＜ stage T2 [95.7％ (22/23)] ＜ stage T3[ 100.0％(10/10) ],stage T1 and stage T2,T3 had significant difference (P ＜ 0.05 ),but stage T2 and stage T3 had no significant difference (P ＞0.05).The GRB2 expression among different gender and age had no significant difference (P ＞0.05).ConclusionsGRB2 has high expression in BTCC tissues.GRB2 expression is related to tumor histological grade andclinical stage.GRB2 plays an important role in the process of BTCC cell proliferation and differentiation.

Objective To evaluate the clinical features of osteoarticular damage of patients with brucellosis,and to enhance the knowledge of clinical doctors.Methods The demographic characteristics,clinical presentations,accessory examination of 265 patients with brucellosis admitted from January 2011 to April 2014 in Ji'nan Infectious Diseases Hospital were retrospectively analyzed.Patients were classified into two groups:Patients with osteoarticular complications and patients without osteoarticular complications.Results Of the 265 patients,forty-six patients (17.36％) had osteoarticular complications.Spondylitis was the most common complication [50.00％ (23/46)],followed by sacroiliitis [41.30％ (19/46)] and peripheral arthritis [26.09％ (12/46)].Sacroiliitis was more common than other types of osteoarticular damage in females [21.74％ (10/46)],whereas in males spondylitis was more common [36.96％ (17/46)].Peripheral arthritis was more common in individuals under the age of 17 years old [4.35％ (2/46)],and sacroiliitis were more common in the ages of 18-60 years old group [23.91％(11/46)] and spondylitis were more common in over 60-year age group [30.43％ (14/46)].Patients with osteoarticular complications showed longer duration of illness from the onset of the symptoms to its diagnosis (median 45 days),compared to those non-osteoarticular brucellosis (median 30 days,Z =5.74,P ＜ 0.05).Conclusion Due to diversity of osteoarticular damage in patients with brucellosis,in case of a patient with long-term fever and osteoarticular symptoms,medical and epidemiological history should be inquired so as to strengthen early diagnosis and treatment of the disease based on combination with radiological findings.