Objective To investigate the etiology spectrum of hand , foot and mouth disease ( HFMD) and to analysis the molecular characteristics of three predominant human enterovirus stains in Qingdao in 2013.Methods The total enterovirus (EV) strains and strains of EV71, CVA16 and CVA6 in throat swabs of HFMD cases were detected by using multiplex real time RT-PCR.The full-length of the viral VP1 genes of the EV strains were amplified and sequenced .The sequences were phylogenetically analyzed by using the MEGA5.0 software package .Results A total of 841 patients with mild HFMD and 107 patients with serious HFMD were recruited in this study and 64 .3%of them were positive for EV .The predominant pathogens were EV71 (44.8%), CVA6 (28.2%) and CVA16 (9.5%) in 2013.CVA6 replaced CVA16 as the second most common pathogen for HFMD , accounting for 42.7% of all pathogens in children aged less than 3 years and 22.2%of all pathogens in the serious patients .The proportions of CVA6 in the etiology spectrum showed a downtrend along with the increasing age of the patients (P<0.001).Phylogenetic analy-sis of the complete VP1 gene sequences showed that all of the EV 71 strains identified in this study belonged to the subgenotype C4 (evolutionary branch C4a) and all of the CVA16 strains belonged to the subgenotype B1 (evolutionary branches B1a and B1b).There were 6 genogroups (A to F) regarding to the VP1 gene of CVA6 and all of the CVA6 strains identified in this study belonged to genogroups A and D .Among the CVA6 strains isolated in Qingdao in 2013, 83.9% belonged to genogroup A, while the rest 16.1% belonged to genogroup D.66.7%of the CVA6 strains isolated in 2012 belonged to genogroup A, while the rest 33.3%belonged to genogroup D .All of the CVA6 strains isolated from year 2008 to 2011 in Qingdao belonged to genogroup D.Conclusion EV71, CVA6 and CVA16 were the prevalent pathogens responsible for the de-velopment of HFMD in Qingdao in 2013.The proportions of CVA6 strains in the etiology spectrum showed a downtrend with the increasing age in children .C4a was the major subtype of EV71 strains circulating in Qingdao in 2013, while B1a and B1b were the major subtypes of CVA16 strains.The pattern of endemic cir-culation of CVA6 strains showed a trend of changing from genogroup D to A from year 2008 to 2013 .

Objective:To explore the predictive efficiency of the timed "up and go" test (TUGT) on the frailty of elderly inpatients with cardiovascular disease and to provide an objective reference for clinical assessment of frailty.Methods:According to the convenience sampling method, a total of 156 elderly patients who were hospitalized in Cardiovascular Department in the Second Affiliated Hospital of Shantou University Medical College from May to July 2019 were selected as research objects. Frailty Phenotype Scale was used to evaluate their frailty, TUGT was used to record their walking function and ROC was used to analyze the predictive efficiency of TUGT on the frailty of elderly inpatients with cardiovascular disease.Results:ROC curve analysis showed that the optimal critical value of TUGT for predicting frailty of patients aged 60 to 69 years was 15.17 s, area under curve ( AUC) was 0.827, 95% CI was 0.727 to 0.902, the sensitivity was 66.67% and the specificity was 98.04%. The optimal critical value of TUGT for predicting frailty of patients aged equal or over to 70 years was 15.26 s, AUC was 0.829, 95% CI was 0.724 to 0.906, the sensitivity was 77.50% and the specificity was 82.86%. Conclusions:TUGT has moderate predictive efficacy in assessment of frailty in elderly inpatients, but it can provide an objective and effective reference for clinical data. Moreover, the test is fast, simple and clinically easy to operate, which can be used as a preliminary screening for frailty in elderly inpatients with cardiovascular diseases.

Objective To investigate effects of magnesium sulfate wet compress nursing on prognosis of child′s scrotum hematoma after indirect inguinal hernia. Methods A total of 54 pediatric children were collected for inguinal hernia operation by convenient sampling method, and random number table method were used to averagely divide patients into control group ( received routine nursing ) and observation group ( given conventional care and magnesium sulfate local wet dressing care) . The child′s length of hematoma vanishing and incidence of complications were compared between two groups. Results In the observation group, the length of hematoma vanishing was 4-10 d, the average time (5. 8 ± 1. 45)d, while the length of hematoma vanishing was 6-14 d, the average (7. 8 ± 2. 11)d in the control group (P<0. 05). There was no incidence of ulcer, local infection and other complications in the observation group whereas 3 cases of ulcer and 1 cases of local infection in the control group (P<0. 05). Conclusions Magnesium sulfate wet compress nursing can shorten the length of child′s scrotum hematoma vanishing after indirect inguinal hernia surgery and reduce the incidence of complications.