Objective To explore the protective mechanism of fructose-1, 6-disphophate on the hypoxic-ischemic brain damage of neonatal rats.Methods Seven-day-old Wistar rats were randomly divided into HIBD control group,saline-treated group, FDP-treated group. FDP-treated group was redivided into groups of high, middle and low dose.The expression of caspase-3mRNA was detected at 24h after HIBD with RT-PCR. Results There were no sifnificant differences of the caspase-3 mRNA expression among the saline-treated group, HIBD group and low dose FDP-treated group(P >0.05).The expressions of caspase-3 mRNA in the middle and high dose FDP-treated groups decreased significantly as campared to the above three groups (P < 0.05), but there was no significant difference between the two groups (P > 0.05). Conclusion The neuronal protective mechanism of FDP might be related to dewnregulate the gene expression of caspase-3 in brain tissue,and decrease the apoptosis of neuron after HIBD.

The main characters and peroxidase isozymes of Coix lacryma-jobi L. and C.lacryma-jobi var.friumentacea Makino and their F1 hybrid were analyzed. Results showed that the plant height, area of leaves per plant, growth performance, and photosynthetic characteristic of F1 hybrid all exceedcd its parents. Bands of iso-peroxidase were different between F1 hybrid and itsparents. There were more band s of isoperoxid ase in the C.lacryma-jobi var. friumentacea Makino than in C.lacryma-jobi L..This is the first report to show the inheritance regularity of stigma colour which was controlled by two complementation genes.

Autophagy is induced by a variety of signals during ischemia and reperfusion.Autophagy has been shown to protect cardiac cells and to reduce the cell loss,but it also has been shown that enhanced autophagy contributes to cell death during I/R.

Objective To detect the serum levels of carbohydrate antigen 199(CA199),carbohydrate antigen 125 (CA125)and carcino-embryonic antigen(CEA)in different population,and to explore their significances in diagnosis of pancreatic cancer.Methods The serum levels of CA199,CA125 and CEA in 56 patients with pancreatic cancer (pancreatic cancer group ), 5 6 patients with benign pancreatic disease (benign pancreatic disease group ) and 56 healthy volunteers(normal control group)were detected,then the positive rates of CA199,CA125 and CEA and the sensitivities and specificities of CA199,CEA and CA125 for the diagnosis of pancreatic cancer were compared among three groups.Results The levels of serum CA199,CA125 and CEA of the patients in pancreatic cancer group were significantly higher than those in benign pancreatic disease group and normal control group (P<0.05). The positive rates of CA199,CEA and CA125 in pancreatic cancer group were 87.50 %,71.42%,and 60.71%, respectively. The positive rates of the three tumor markers of the patients in pancreatic cancer group were obviously higher than those in other two groups (P<0.05).The sensitivity of CA199(87.50%)was the highest,while the specificity of CEA (9 1.9 6%)was the highest. The method of parallel combined testing increased the diagnostic sensitivity to 95.12% and negative predictive value to 90.47%,but its specificity and the positive predictive value were decreased. Serial combined examination increased the diagnostic specificity to 92.86% and the positive predictive value to 89.19%,but its sensitivity and negative predictive value were decreased.Conclusion Combined detection of serum levels of CA199,CA125 and CEA has higher sensitivity and specificity than single-marker detection in the diagnosis of pancreatic cancer.

Objective To investigate the expression of matrix metalloproteinases(MMP) related to oncogene C-myc and tumor differentiation of cutaneous squamous cell carcinoma (CSCC). Methods Expression of MMP2,MMP9 and C-myc was detected by immunohistochemistry in 68 paraffin embedded specimens of CSCC. Results Distribution of MMP2 and MMP9 was demonstrated within cytoplasm of tumor and stromal cells, especially for those cells at the interface of tumor and interstitial tissues. No expression of MMP2 and MMP9 was observed in the horny centers of tumor cell nests. Expression of MMP2 and MMP9 increased along with the decreasing of the differentiation of tumor tissue and the increasing of C-myc expression. Conclusions Expression of MMP2 and MMP9 might be a common event for both tumor and stromal cells but parenchymal tumor cells might predominate over the activation of MMP2 and MMP9. Oncogene C-myc protein might promote the secretion of MMP2 and MMP9 by tumor cells.

The problems of some hospital in medical equipment maintenance management were introduced from the aspects of maintenance time, maintenance record, maintenance service and self maintenance. Some measures were proposed including simplifying maintenance process through PDCA, informatizing maintenance record, cooperating with third-party maintainer and establishing self maintenance team, so that precision and informatized medical equipment management can be realized to maximize the benefit of medical equipment management.

0.05).Conclusion The elevation of GH involved in the pathogenesis of diabetic microangiopathy possibly by exacerbation of disorders of glucose and lipid metabolism.

Objective To study the expression of vascular endothelial growth factor(VEGF) mRNA in neonatal rats with HIBD and to explore the function of VEGF in HIBD. Methods Fourty-eight Wistar rats of 7-day-old were assigned randomly to the control or HIBD groups. The left common carotid artery of rats in HIBD group were isolated and ligated, then exposed to hypoxic environment (8%O 2) for two hours. In control group the rat left common carotid artery were isolated, but not ligated and not exposed to hypoxic environment. Animals were killed in 3 h, 12 h, 24 h and 3 d after HIBD. The expression of VEGF mRNA in hippocampus were examined by RT-PCR.Results VEGF164 was the most prominent transcript of VEGF mRNA splice variants. The expression of VEGF mRNA was present in control group, but there were no significant differences between time points ( F =0.176, P =0.911). Levels of VEGF mRNA in HIBD group were higher than that in the control group at all time points( P

Objective To investigate the expression of insulin-like growth factor-Ⅱ( IGF-II ) and its mRNA in human placenta and to evaluate its role in the pathogenesis pregnancy induced hypertension (PIH). Methods The cellular distribution of IGF-Ⅱin normal and PIH placenta was determined by immunohistochemistry. Trophoblasts were digested,separated,and purified from placenta. IGF-ⅡmRNA expression in trophoblasts was detected by RT-PCR. The mean density was determined by image analysis technique. Results (1) IGF-Ⅱwas mostly found in syncytiotrophoblast,cytotrophoblast and amnio-chorion. The strongest immunoreactivity was observed in the syncytiotrophoblast and cytotrophoblast. (2)The mean density of PIH placenta (0.324?0.042) was significantly lower than that of normotensives (0.360?0.072,P

The clinical manifestations, laboratory tests and treatment of 5 cases of simple virilizing with 211-hydroxylase deficiency were analyzed. The average age was 17 at the first hospital visit. The manifestation included abnormal external genitalia at birth, no secondary sexual characteristics developed during adolescence and no menstrual cycles, and their near-adult height (NAH) was affected. The chromosome karyotype of the patients was 46 XX. The CT scans showed bilateral adrenal hyperplasia. The testosterone levels were (665 ± 334 ) μg/L, which were reduced to (81 ± 25 ) μg/L after dexamethasone suppression. The levels of adrenocorticotropic hormone were 123 (57 - 563) ng/L and ＜ 5 ng/L respectively before and after dexamethasone suppression test. Early diagnosis and early treatment is particularly important for improvement of the disease outcomes.