AIM: To explore the effects of advanced gly cosyla tion end products (AGEs) on connective tissue growth factor (CTGF) mRNA expressi on and extracellular matrix (ECM) synthesis in cultured rat renal mesangial cel ls. METHODS: Rat renal mesangial cells were cultured in vitro un der standard conditions. The content of fibronectin (FN) and type Ⅳ collagen (ColⅣ ) were determined with ELISA after stimulation by AGE-BSA and BSA, respectively. CTGF mRNA expression was assessed by RT-PCR. RESULTS: CTGF mRNA was significantly increased by AGE-BSA in cul tured mesangial cells in a time-and dose-dependent manner compared with BSA cont rol (P

Objective To investigate the relation between plasma macrophage migration inhibitory factor(MIF) concentration and the activity of activator protein-1(AP-1) in peripheral blood and coronary heart disease(CHD),and their relation with coronary arteriosclerosis plaques in patients with CHD.Methods One hundred and forty two patients were divided into coronary heart disease(CHD) group(n=107) and control group(n=35) according to coronary angiographic(CAG) result.The CHD group was further divided into stable angina pectoris(SAP) group(n=33) and acute coronary syndrome(ACS) group(n=75) according to clinical manifestations.In addition,according to the type of coronary lesions,the CHD group was further divided into the type A lesion(n=30) group,type B group(n=48) and type C group(n=29).According to the degree of coronary stenosis,the CHD group was also divided into mild stenosis group(n=21),moderate stenosis group(n=31) and severe stenosis group(n=55).The amount of Phospho-c-Jun in lysate and plasma MIF concentration were measured by enzyme-linked immunosorbent assay(ELISA).The results of Phospho-c-Jun was demonstrated with absorbance.Which reflect the amount of AP-1.Results The absorbance of Phospho-c-Jun in the CHD group was higher than that in the control(1.43?0.33 vs 0.71?0.13,P

AIM: To investigate the effects of advanced glycation end products (AGEs) on the expression of plasminogen activator inhibitor-1 (PAI-1) in rat mesangial cells and its relationship with extracellular matrix accumulation. METHODS: Rat mesangial cells were treated with AGE-modified bovine serum albumin or native bovine serum albumin. Normal mesangial cells without any treatments were used as control. Fibronectin (FN), collagen Ⅳ, PAI-1 protein contents were detected by ELISA. PAI-1 mRNA was analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR). RESULTS: AGEs (0-200 mg/L) did not influence mesangial cells proliferation, but stimulated FN , collagen IV and PAI-1 contents in mesangial cell cultured medium in different degrees. AGEs also increased PAI-1 mRNA expression. CONCLUSION: AGEs increase the expression of PAI-1 in rat mesangial cells. AGEs may reduce ECM degradation through increasing PAI-1 expression, which may be one of the mechanisms of ECM accumulation in diabetic nephropathy.

Objective To investigate the effects of advanced glycation end products(AGEs) on expression of peroxisome proliferator-activated receptor-?(PPAR?) mRNA in rat renal cortex.Methods Normal rats were given tail vein injection with either AGE-modified rat serum protein(AGEs)or AGE-RSP followed by intraperitoneal injection of AG(AGEs+AG)or native rat serum protein(native RSP).Normal rats without any treatment were as controls(Control).PPAR-? mRNA expression was analysed by reverse transcriptase-polymerase chain reaction(RT-PCR).Results PPAR-? mRNA expressed in all rat kidney cortex.There was a decrease for PPAR-? in mRNA levels in the renal cortex of AGEs-treated rats(P

Objective To investigate the relation between activator protein-1(AP-1)and coronary atheroselerotic changes and the potential role of AP-1 in the stabilization of atherosclerotic plaques in patients with coronary heart disease(CHD).Method 142 patients were included in this study and divided into CHD group(107)and control group(35)according to coronary angiography(CAG).The CHD group was further divided into a stable angina pectoris(SAP)group(32)and all acute coronary syndrome(ACS)group (75)according to the clinical manifestations.In addition,the CHD group was divided into A type group,B type group and C type group according to the standard of ACC/AHA coronary change in 1988.Meanwhile,the CHD group was further divided into light stenosis group,moderate stenosis group and severe stenosis group according to the degree of coronary lesion.The lysate of cells was obtained through lysis of the leucocyts from peripheral blood with cell lysis buffer.The amount of Phospho.c-Jun in lysate was measured with enzyme-linked immunosorbent assay(ELISA).The results were demonstrated with absorbance,which reflects the amount of AP-1.Results The main coronary changes in the SAP group were A type(68.7%)and the changes were mainly of light degree(53.1%);the main coronary changes in the ACS group were B type(52.0%)or C type(37.3%)and the changes were mainly of heavy degree(66.7%).The absorbance of Phospho-c-Jun in CHD group was significantly higher than that in the control subjects (1.43±0.33 vs 0.71±0.13,P＜0.001).The absorbance of Phospho-c-Jun in the ACS group was significantly higher than that in the SAP group(1.56±0.28 vs 1.14±0.25,P＜0.001).The absorbance of Phospho-c-Jun increased gradually from A type group to C type group(1.18±0.27 vs 1.42±0.26 vs 1.71±0.27,P＜0.001)and from light stenosis group to severe stenosis group(1.09±0.20 vs 1.37±0.26 ys 1.60±0.29,P＜0.001).Conclusion There is a significant relationship between AP-1 and coronary atherosclerotic changes.AP-1 may be a factor that can predict coronary arteriosclerotic progression and stability of the plaque.

Objective To investigate the relationship between the plasma adiponectin concentration and coronary arteriosclerosis change in patient with coronary heart disease(CHD).Method 142 patients were divided into CHD group and control group according to the Coronary Angiography(CAG).CHD group were further divided into stable angina pectoris(SAP)subgroup and acute coronary syndrome (ACS)subgroup according to the clinical property.According to the type of coronary change,CHD group wag divided into A type group, B type group and C type group,meanwhile according to the degree of coronary lesion,CHD group was divided into light stenosis group, moderate stenosis group and severe stenosis group.The plasma adiponectin concentration was measured by ELISA.Results The plasma adiponectin concentration in CHD group was significant lower than that in control group.The plasma adiponectin concentration in ACS subgroup was significant lower than that in SAP subgroup.The plasma adiponectin concentration decreased gradually from A type group to C type group and from light stenosis group to severe stenosis group(P＜0.001).Conclusions Adiponectin is a negative regulatory factor of coronary atherosclerosis,and Hypoadiponectin may be used to predict the change of coronary arteriosclerosis and the stability of plaque.

Objective To explore the association between plasma resistin levels and acute coronary syndrome. Methods Four hundred patients were divided into coronary heart disease (CHD) group（310）and control group（90）according to the coronary Angiography (CAG). And CHD group was divided into ACS subgroup（n＝217）and SAP subgroup（n＝93）according to the clinical information. 85 cases in CHD group were underwent 64-slice spiral computed tomography coronary artery imaging. The severity and extent of coronary lesions were analyzed by CAG and graded by means of Gensini coronary score system. Resistin level in plasma of all patients was determined by enzyme linked immunosorbent assay. Results Resistin levels in CHD group［(889.1±248.2)pg/ml］ were significant higher compared with the control group［(261.6±111.9)pg/ml］ (P＜0.05), and resistin levels in ACS subgroup［(1260.0±368.0)pg/ml］ were much higher than that in SAP subgroup［(518.3±128.4)pg/ml］ (P＜0.05). Conclusions The resistin levels of patients with acute coronary syndrome increased significantly and might be associated with the vulnerable plaque. Resistin levels and 64 slice spiral computed tomography coronary artery imaging can be used to detect the vulnerable plaque in CHD patients.

Purpose To build up the Potassium Sodium Dehydroandroan Drographolide Succinate enteric coating sustained-release pellets delivery system by aqueous dispersion coating technique. Methods 1. Adopting MCC as vehicle, SiO_2 as antisticking agent, appropriate amount of 40% ethanol, preparing the core of pellets by extrude-spheronization method and the formulation and manufacturing process were optimized by orthogonal design. 2. Preparing the coating material with Eudragit L 30 D, which is used as pore-forming agent, EC as blocker and PEG6000 as plasticizer. The pellets were coated by fluid-bed coating method. Results 1. The optimal formulation and manufacturing process of pelltes' core were as follows: drug: MCC: SiO_2 = 7:7:5, extruding rate: 1 080 r/min, rounding rate: 960 r/min, rounding time: 5 min. 2. After the addition of EC: Eudragit L = 35:65, the plasticizer is 1.71 % and weight gain is 5% . The release in the gastric model fluid(pH 1.0) < 10% , and complete release ( > 80% ) in the enteric model fluid(pH 6.8) was in two hours. The release behavior accords with the regulations on the release rate of enteric preparation in ChP. Conclusion By adjusting the formulation and the parameters of the process of pellet and coating, we can make enteric coating Potassium Sodium Dehydroandroan Drographolide Succinate sustained-release pellets. All this accords with the regulation of pharmacopedia in vitro release.

Objective To observe the clinical effect of inserted central catheter (PICC) tip localization guided by ultrasound combined with endocaridal electrogram (ECG).Methods A total of 115 PICC catheter patients were as research objects, and ultrasound combined with endocaridal electrogram guided PICC tip positioning were carried out for these patients, which was compared with tube X-ray results, its application value in the location of the tip of PICC was analyzed.Results Ultrasound combined with intracavitary electrocardiogram guided PICC tip localization rate was 95.7%, ectopic rate was 4.3%, while the X-ray results suggested that the PICC tip localization rate was 97.4%, and ectopic rate was 2.6%, but there was no statistically significant difference (P>0.05);P wave prediction and X-ray coincidence rate of ultrasound combined with intracavitary electrocardiogram guided PICC tip positioning was 98.3%, Kappa test showed a better consistence(P<0.05).Conclusion The success rate of PICC tip localization guided by ultrasound combined with endocaridal electrogram is high, which can reduce the adverse effects of the tip dislocation, and it can be used as priority.

Objective To observe the clinical effect of inserted central catheter (PICC) tip localization guided by ultrasound combined with endocaridal electrogram (ECG).Methods A total of 115 PICC catheter patients were as research objects, and ultrasound combined with endocaridal electrogram guided PICC tip positioning were carried out for these patients, which was compared with tube X-ray results, its application value in the location of the tip of PICC was analyzed.Results Ultrasound combined with intracavitary electrocardiogram guided PICC tip localization rate was 95.7%, ectopic rate was 4.3%, while the X-ray results suggested that the PICC tip localization rate was 97.4%, and ectopic rate was 2.6%, but there was no statistically significant difference (P>0.05);P wave prediction and X-ray coincidence rate of ultrasound combined with intracavitary electrocardiogram guided PICC tip positioning was 98.3%, Kappa test showed a better consistence(P<0.05).Conclusion The success rate of PICC tip localization guided by ultrasound combined with endocaridal electrogram is high, which can reduce the adverse effects of the tip dislocation, and it can be used as priority.