OBJECTIVE To observe the antibacterial effect of the foot-ulcer-cure ointment a compound traditional chinese materia medica(TCMM) preparation manufatured on ulceration of(diabetic) rat,in order to give the basis for clinical treatment.METHODS The total(amount) of bacteria and amount of G~-bacteria and the quota of wound healing after the ointment given to the ulceration surface of(diabetic) rats were observed.RESULTS At the third and seventh day,the total amount of(bacteria) and amount of G~-bacteria of the experiment group were remarkably less than the first day and the control group;the speed rate of the wound healing was faster than the control one.CONCLUSIONS The foot-ulcer-care ointment of TCMM has excellent antibacterial functions to diabetic ulceration.It is worth for popularization.

Objective To establish a multiple PCR method that can be used to spontaneously detect five kinds ofpathogens such as NG,MH,MG,CT and UU.Method With the fluorescence-quantitative PCR technique in conjunction with another detection technique as the golden standard,evaluation was conducted on the sensitivity,specificity,accuracy and repeatability on the detection of 5 kinds of STD pathogens using single-tube multiplex PCR.Result The sensitivity,specificity and match rate of the method ale 10-9fg/μl,100%,97.8%respectively,and the repeatability of 5 continuous days of 20 clinical specimens is good.Conclusion Single-tube multiplex PCR technique provides a new method to detect 5 kinds of STD pathogens.

Objective To evaluate effectiveness of family support on foot care education in older patients with diabetes .Methods A quasi-experimental , interrupted time series with comparison group design was conducted.Finally, according to their level of family support 42 patients were assigned to the two groups .The patients in two groups received a 30 minutes education session individually , and one family member of each patient of the intervention group with high level of family support was educated at the same time .The Nottingham Assessment of Functional Foot care ( NAFF ) and The Multidimensional Scale of Perceived Social Support ( MSPSS ) were used to evaluate the effectiveness of education and the level of family support over time . Results There was no significant difference between the two groups before the two group of patients ( P >0.05).At the three and the six month of follow-up, the median scores of the intervention group were 66.91 and 68.19.The patients in the intervention group with high level of family support demonstrated a significant improvement in NAFF (Z =-2.782, -4.997, respectively;P <0.01).The intervention group indicated significantly better performance over time in MSPSS (χ2 =41.932,P=0.001), but it did not showed in control group (Z=1.228,P =0.874).Conclusions The family support enhanced effectiveness of education and sustained health foot care behavior .The findings of this study are important for nurses and diabetes educators to improve the teaching and improve foot care of the elderly with diabetes .