1.Investigation in cognition of nursing disputes of undergraduates and college nursing students in internship in Changchun city and countermeasures
Jishuang ZHAO ; Purui YANG ; Wenling WANG ; Guifang YUAN ; Xin PENG
Chinese Journal of Practical Nursing 2013;29(27):4-6
Objective To discuss the cognition of the nursing disputes in undergraduates and the college nursing students in internship in Changchun and to provide some basis for them to improve the nursing quality in clinic.Methods The investigation was carried out to survey the cognition of the nursing disputes in 266 undergraduates and the college nursing students in internship in Changchun,and the results underwent analysis.Results Most undergraduates and college nursing students held the opinion that at present the relationship between patients and nurses was on a tight rope.94 people accounting for 72.3% in undergraduate students,89 people accounting for 65.4% in the college nursing students.Relevant factors which caused nursing disputes were related with the different education backgrounds.Conclusions We should make clear the causes of nursing disputes,and put forward the corresponding proposals according to nursing students with different education degrees.
2.Characteristics of Subjective Quality of Life and Family Environment in Children with Tic Disorders Combined with and without Attention Deficit Hyperactivity Disorder
Yuan WU ; Guifang KUANG ; Ying XIA ; Jinna XIE ; Zhongyu HENG
Chinese Journal of Rehabilitation Theory and Practice 2011;17(12):1170-1172
Objective To explore the characteristics of subjective quality of life and family environment in children with tic disorder (TD) combined with and without attention deficit hyperactivity disorder (ADHD). Methods The Inventory of Subjective Life Quality (ISLQ) for Child and Adolescent and Family Environment Scale-Chinese Version (FES-CV) were used to assess the subjective quality of life and family environment in 60 children with TD combined with ADHD (observation group) and 60 children with TD not combined with ADHD (control group). Results The scores in peer interaction, school life, self-cognition, depression experience, overall satisfaction of the observation group were lower than the control group (P<0.05) in ISLQ; the scores in intellectual-cultural orientation, cohesion and organization of the observation group were lower than the control group (P<0.05) in FES-CV. Conclusion Children with TD combined with ADHD had low subjective quality of life and poor family environment.
3.Characteristics of Subjective Quality of Life and Family Environment in Children with Tic Disorders Combined with and without Attention Deficit Hyperactivity Disorder
Yuan WU ; Guifang KUANG ; Ying XIA ; Jinna XIE ; Zhongyu HENG
Chinese Journal of Rehabilitation Theory and Practice 2011;17(12):1170-1172
Objective To explore the characteristics of subjective quality of life and family environment in children with tic disorder (TD) combined with and without attention deficit hyperactivity disorder (ADHD). Methods The Inventory of Subjective Life Quality (ISLQ) for Child and Adolescent and Family Environment Scale-Chinese Version (FES-CV) were used to assess the subjective quality of life and family environment in 60 children with TD combined with ADHD (observation group) and 60 children with TD not combined with ADHD (control group). Results The scores in peer interaction, school life, self-cognition, depression experience, overall satisfaction of the observation group were lower than the control group (P<0.05) in ISLQ; the scores in intellectual-cultural orientation, cohesion and organization of the observation group were lower than the control group (P<0.05) in FES-CV. Conclusion Children with TD combined with ADHD had low subjective quality of life and poor family environment.
4.Effects of curcumin photodynamic therapy on notch signaling pathway in cervical cancer cells
Guifang HE ; Qing XIANG ; Zhihua CHEN ; Yuan ZHANG ; Yali YUAN ; Wenyan YANG
Chinese Journal of Geriatrics 2017;36(8):915-919
Objective To explore the mechanism of notch signaling pathway in the treatment of cervical cancer with notch receptor blocker(DAPT).Methods After treatment of Me180 cells with different concentrations of DAPT combined with curcumin-photodynamic therapy (PDT),3-(4,5 Dimethylthiazlo-yl)-2,5 Dimethylthiazol-2-yl)-2,5 dipheny tetrazo lium bromide(MTT)assay was used to detect the effect on the proliferation of Me180 cells,annexin V-FITC/PI combined with flow cytometry was used to detect the effect on apoptosis,and real-time fluorescence quantitative PCR and Western blot were used to detect the mRNA and protein expressions of Notch1 gene respectively.Results Both DAPT(1.0 μmol/L)and curcumin-PDT groups could inhibit the proliferation and induce apoptosis of cervical cancer cell line Me180,and the synergistic effect of the two groups was significant (P<0.05 or<0.01).DAPT and curcumin-PDT group reduced the mRNA expression level of Notch1 in cervical cancer Me180 cells with inhibition ratio of 40.0% and 32.3% respectively.DAPT and curcumin-PDT group inhibited the protein expressions of Notch1,nuclear factor-kappa B(NF-kB),vascular endothelial growth factor (VEGF),and the synergistic effect of the two groups was statistically significant.Conclusions Both DAPT and curcumin-PDT may effectively block the conduction of Notch signaling pathway,which is associated with down-regulation of the expression of Notch1 and NF-kappa B.Notch signaling pathway may be one of the targets of curcumin photodynamic therapy.
5.Total flavonoids of Epimedium attenuate aging-related inflammation in rat brain by inhibiting MAPK/NF-κB signaling pathway
Laixin SONG ; Changcheng ZHANG ; Ting WANG ; Zhencai LIU ; Guifang HAN ; Ding YUAN ; Haixia ZHAO
Chinese Pharmacological Bulletin 2017;33(1):84-89,90
Aim To investigate the effect of the total flavonoids of Epimedium (TFE)on MAPK/NF-κB sig-naling pathway and the inflammatory reaction in the hippocampus of natural aging male rats.Methods The morphological changes of the hippocampus com-posed of three areas (CA1 ,CA3 and DG)were ob-served using haematoxylin-eoin (HE ) staining.The protein expression levels of senescence-associated pro-tein p21,apoptosis-related proteins Bax and Bcl-2, nuclear transcription factor-κB p65 (NF-κB p65 )and its downstream inflammatory factors TNF-α,IL-1βand COX-2,and MAPK signaling pathway-related proteins (ERK1/2,p-ERK1/2,JNK,p-JNK,p38MAPK,p-p38MAPK)in hippocampal were detected by Western blot.Results Compared with natural aging group, TFE obviously improved the morphology and structure of hippocampal neurons,and the nerve cells arranged neatly and closely. Furthermore, TFE significantly downregulated the protein expression levels of p2 1 and Bax,upregulated the protein expression levels of Bcl-2 and the ratio of Bcl-2/Bax,and reduced the expression of NF-κB p65 and of its downstream inflammatory fac-tors TNF-α, IL-1β, COX-2, and MAPK signaling pathway-related proteins (p-ERK1/2,p-JNK and p-p38 MAPK ) in hippocampus of natural aging rats. Conclusions TFE effectively protects against inflam-matory reaction in brain aging of SD male rats.The mechanism is related with inhibition of NF-κB nuclear translocation and reduction of its downstream inflamma-tory cytokines expression by inhibiting MAPK signaling pathway activation.
6.Serum screening of celiac disease in Chinese adults with diarrhea-predominant irritable bowel syndrome in Hubei, China
Hongling WANG ; Linjie LUO ; Jiguang KOU ; Anlong YUAN ; Guifang YANG ; Min WANG ; Bing XIA
Chinese Journal of Internal Medicine 2013;(1):38-41
Objective To screen the prevalence of celiac disease with serologic markers in the central Chinese population,specifically in patients with chronic diarrhea-predominant irritable bowel syndrome (D-IBS).Methods A total of 282 adult patients with D-IBS were selected based on ROME Ⅲ criteria with 296 age and sex matched consecutive healthy individuals as controls.A gluten-free diet (GFD) was advised in subjects positive for IgA/IgG anti-htTG/DGP antibodies and the serologic antibodies were retested after the GFD.Results Among the 578 study subjects,five D-IBS patients (5/282,1.77%) and two healthy controls (2/296,0.68%) were positive for anti-htTG/DGP antibodies.Among the seven positive cases,one was lost to follow-up and only four were evaluated during GFD therapy for an average of 5.2 months with clinical and/or serological manifestations improved.Conclusions The prevalence of celiac disease may not be uncommon in China.Compared with the healthy population,patients with D-IBS tend to be affected more.Thus,it is significantly important to conduct routine screening for celiac disease in patients with D-IBS.
7.Feasibility Study on Blood Concentration Determination of Peripiocoside in Bawei Jiangu Tablet by Chemiluminescence Enzyme Immunoassay
Yingping CHEN ; Yuan TIAN ; Yi HAN ; Bo HAN ; Li GONG ; Guoxin LI ; Guifang LIU ; Xuewen ZHOU ;
Traditional Chinese Drug Research & Clinical Pharmacology 2000;0(06):-
Objective To establish the clinical pharmacokinetics research method for Bawei Jiangu Tablet.Methods Drug blood concentration was estimated through analyzing the ingredients and preclinical pharmaceutical research results of Bawei Jiangu Tablet.Results Corresponding cardiac glycoside value in serum of animals treated with oral use of Bawei Jiangu Tablets and cortex periplocae extracts can be detected by drug monitor system of digoxin immunoreaction, which was similar to that in animals treated with digoxin.Corresponding cardiac glycoside value had also been detetected in serum of subjects in phaseⅠclinical tolerance trial,which was related to the time and dosage of administration.Clini- cal negative control trial showed that the reaction was specific.Conclusion Pharmacokinetics study on target of toxic components with cross-reaction of periplocoside is feasible through drug monitor system of digoxin immunoreaction.
8.Effect of estrogen on proliferation of astrocytes in hippocampus of mice following middle cerebral artery occlusion
Shujuan WANG ; Panpan ZHANG ; Xiaodong YUAN ; Guifang WANG ; Xiulan ZHAO ; Wenxing LIU ; Gang LI ; Jianghua SONG ; Qianqian JIANG
Chinese Journal of Geriatric Heart Brain and Vessel Diseases 2019;21(5):525-529
Objective To study the effect of estrogen on proliferation of astrocytes in hippocampus of mice following middle cerebral artery occlusion(MCAO).Methods One hundred and eight Kunming mice were randomly divided into estrogen group(n=54)and saline group(n=54).The animals in two groups underwent right MCAO with tissue samples taken at 3,6,12,24,48and 72h after MCAO.The ischemic site was detected and the ischemic size was measured with TTC staining,the damage of neurons in hippocampus was assayed with HE staining,the expression of GFAP in hippocampal astrocytes was detected with immunohistochemical staining.Results The cerebral infarction size was significantly smaller in estrogen group than in saline group at different time points after MCAO(P<0.05,P<0.01)especially at 12hafter MCAO(31.50%±3.36%vs 54.50%±5.68%,P=0.019).The damage of hippocampal neurons aggregated with the prolonged ischemia time in two groups and was milder in estrogen group than in saline group at the same time points.The expression level of GFAP positive cells in bilateral hippocampal areas was higher when the ischemia time was prolonged and was significantly higher in ischemic hippocampus of estrogen group than in that of control group except at 6hin CA3ischemic area(P<0.05).Conclusion Estrogen can protect mice against focal cerebral ischemia,stimulate the genesis of astrocyte synapses,alleviate neuronal damage after ischemia,and can thus reduce the size of cerebral infarction.
9.Application of multispectral imaging analysis system in quantitative analysis of immunohistochemical images of breast cancer.
Wenlou LIU ; Linwei WANG ; Jiamei CHEN ; Jingping YUAN ; Fang YANG ; Qingming XIANG ; Guifang YANG ; Aiping QU ; Juan LIU ; Yan LI
Chinese Journal of Pathology 2015;44(10):743-746
10.Differences in shapes and properties and microscopic frameworks of wild and cultivated Radix Salviae Miltiorrhizae from different regions.
Guifang FU ; Xuefeng FENG ; Xiaoguang GE ; Xirong HE ; Zhigang WU ; Jingyu YANG ; Jin'e WANG ; Xiaoming LI ; Yuan YUAN
China Journal of Chinese Materia Medica 2010;35(10):1235-1238
OBJECTIVETo compare the differences in shapes and properties and the microscopic frameworks of the wild and cultivated Radix Salviae Miltiorrhizae from different regions.
METHODThe differences in the shapes and properties, the characters of transverse sections, the powder and disintegrated tissue of roots were compared using microscopic measurement and statistics analysis.
RESULTThe wild Radix Salviae Miltiorrhizae had several long cylinder roots with rough flaky squama skin and brown red or wine culour, the cultivated had root of many branch with cling skin and brick-red or chestnut culour. The difference of microscopic histological structure was that the xylem vessel of wild Radix Salviae Miltiorrhizae had bunched vessel with the rank form of big diameter alternating with small diameter, and had stone cell on samples from some producing region, the xylem vessel of the cultivated had no bunched vessel and no stone cell with the rank form of tangential radial. Radix Salviae Miltiorrhizae cultivated in Sichuan Province is called original-region medicinal materials and named Chuandanshen. Chuandanshen had the differences with the Salviae Miltiorrhizae Radix cultivated in other region. The root of Chuandanshen had 1.2 cm diameter, and was bulky and fat with solid fabric and the fracture with brownish yellow color and cutin-alikeness, its xylem vessel of transverse section of root was thin with the rank form of tangential radial, and 19-24 vascular bundle and a few wood fiber.
CONCLUSIONSalviae Miltiorrhizae Radix of the wild and the cultivated, of the original-region (Chuandanshen) and the other-region, have the differences in the shapes and properties, and the microscopic frameworks. The character can be identified by the differences in the shapes of medicinal materials, and the rank form of vascular bundle of transverse section of root.
China ; Microscopy ; Quality Control ; Salvia miltiorrhiza ; anatomy & histology ; chemistry ; growth & development