OBJECTIVE:To study the safety of Jaundice-abating Chinese Herbal Lotion for External use through animal skin susceptibility test so as to provide references for its clinical application.METHODS:Skin and systemic susceptibility tests were conducted in accordance with the pertinent standards issued by our country.RESULTS:Neither skin allergic reaction nor systemic allergic reactions were noted for jaundice-abating Chinese Herbal Lotion for external use.CONCLUSION:Jaundice-abating Chinese Herbal Lotion for external use meets the clinical requirements in the skin susceptibility test.This drug was proved to be safe in the local or systemic application,and it can be used in the clinic through systemic tub bath.

Objective To explore the methodology of the preventing the absorption after autog-enous fat granule mammaplasty, and the main point of the operative methods.. Methods The clinical data were reviewed in 30 cases of patients with liposuction combined with fat-grafting injection for breast mammaplasty in the past four years. The tumescent technique was used in liposuction and the volume of fat tissue aspirated was between 500 ml and 3250 ml. Using appropriate way we prepared pure fat granules and then evenly injected into the layer beneath the pectoralis major muscle, submam-mary layer and subcutaneous layer of the breast, respectively. Results The effect of the method was satisfying because sucking area was significantly decreased. From six months to 10 months follow-up, grafts showed no obvious absorption. The breast looked symmetry. Conclusion Fat grafting injection is a practicable method for breast mammaplasty. The key point is to use appropriate way to get pure fat granule and to prevent infection. It deserves to be recommended.

Objective To explore the effect of non nutrition sucking and massage on gastrointestinal function in very low birth weight infants in NICU.Methods A total of 62 very low birth weight infants in NICU of our hospital were selected as study subjects.According to the order of entering NICU time,they were divided into observation group (33 cases) and control group (29 cases).The control group only treated with gastric tube feeding and the observation group was treated with non nutrition sucking and massage on the basis of the control group.The changes of body weight and gastrointestinal function in two groups were analyzed and compared.Results There was no significant difference in birth weight of the neonates in two groups at birth (P ＜0.05).At 7th,14th and 28th day after birth,the body mass of the observation group were significantly higher than those of the control group (P ＜ 0.05).The indicators of defecation and feeding in the observation group were better than that of the control group (P ＜ 0.05).In addition,the incidence rates of vomiting,abdominal distention,gastric retention and pathoglycemia of the observation group during feeding period were significantly lower,and the symptoms improved times were significantly lower than that in the control group (P ＜ 0.05).Conclusion Non nutrition sucking and massage can promote maturation of gastrointestinal function,reduce the occurrence of feeding intolerance,accelerate the early growth and development of very low birth weight infants.

Objective To explore the effect of non nutrition sucking and massage on gastrointestinal function in very low birth weight infants in NICU.Methods A total of 62 very low birth weight infants in NICU of our hospital were selected as study subjects.According to the order of entering NICU time,they were divided into observation group (33 cases) and control group (29 cases).The control group only treated with gastric tube feeding and the observation group was treated with non nutrition sucking and massage on the basis of the control group.The changes of body weight and gastrointestinal function in two groups were analyzed and compared.Results There was no significant difference in birth weight of the neonates in two groups at birth (P ＜0.05).At 7th,14th and 28th day after birth,the body mass of the observation group were significantly higher than those of the control group (P ＜ 0.05).The indicators of defecation and feeding in the observation group were better than that of the control group (P ＜ 0.05).In addition,the incidence rates of vomiting,abdominal distention,gastric retention and pathoglycemia of the observation group during feeding period were significantly lower,and the symptoms improved times were significantly lower than that in the control group (P ＜ 0.05).Conclusion Non nutrition sucking and massage can promote maturation of gastrointestinal function,reduce the occurrence of feeding intolerance,accelerate the early growth and development of very low birth weight infants.

Objective To standardize the procedure and ensure the safety of oral administration,and to achieve the "three-accurate",that is right medicine,right time,and right patients． Methods The concept of "Health Care Failure Mode and Effect analysis" was used to analyze the procedure of in-patients' oral administration,and adjust the system according to insecurity factors to improve the hospital oral administration system constantly． Results The systematic reconstruction of the oral administration improved nurses' and patients' satisfaction,reduced the error rate of in-patients' oral administration and improved the safety of oral administration． Conclusion The application of"Health Care Failure Mode and Effect analysis" to forward-looking analysis in-patient oral administration system,combined with the actual situation of our hospital to develop and implement plans are the guarantees of oral administration security.

OBJECTIVETo explain a method for lower face and anterior neck reconstruction.

METHODSTissue expanders were implanted subcutaneously beside the defect. When the skin was properly expanded, the bipedicled expanded skin flap was created and transferred to the lower face and the anterior neck. If the defect can not be repaired by one stage, the expanded skin was re-expanded for the rest.

RESULTS11 patients have been treated with this method since 1990. All flaps survived and all the defects have been successfully repaired with satisfactory results.

CONCLUSIONThe bipedicled expanded flap, which provided good blood supply, improved the survival rate of the flap and reduced the incision scar on the face, is an effective surgical procedure.

Adolescent ; Adult ; Child ; Face ; surgery ; Female ; Humans ; Male ; Middle Aged ; Neck ; surgery ; Reconstructive Surgical Procedures ; methods ; Surgical Flaps ; Time Factors ; Treatment Outcome

OBJECTIVETo evaluate the effect of external use of papaverine cream on enhancement of tissue expansion.

METHODS8 mini pigs were randomly divided into 4 groups. During inflation, papaverine was given externally in group A, through a delivery system in group B, though intramuscular injection in group C. Papaverine was not given in group D. The time of inflation, the expansion rate, the surviving length of expanded flaps and the papaverine concentration in the fibrous capsule in each group were examined. Histologic and electroscope examinations of the expanded tissue were made.

RESULTSThe average inflation time was 28.9 +/- 4.5 days in group A, 34.0 +/- 2.6 days in group B, 37.6 +/- 4.8 days in group C and 38.5 +/- 3.5 days in group D. There was significant difference between each group(P < 0.05). The average surviving length of expanded flaps in group A was 13.67 +/- 1.28 cm, 11.07 +/- 0.88 cm in group B, 10.79 +/- 0.49 cm in group C, and 9.49 +/- 0.77 cm in group D. There was significant difference between each group (P < 0.01). Histologic examinations of the expanded tissue suggested that there were no significant differences in thickness of epidermis, derma and fibrous capsule among the four groups. Electroscope examinations of the myofibroblast in fibrous capsule showed that there were smaller nuclei, fewer microfilaments and fewer mitochondrias and rougher endoplastic reticulume in group A than those in other groups. The concentration of papaverine in fibrous capsule of group A was 3.5 +/- 1.2 micrograms/g. Papaverine was detected neither in the skin of other groups nor in the blood of all groups.

CONCLUSIONS1. Papaverine cream can permeate through skin and maintain high concentration and continuous effect in local tissue. 2. External use of papaverine cream can inhabit the function of myofibroblast in fibrous capsule, increase inflation rate, accelerate expansion process, and improve microcirculation of the expanded skin with a result of prolongation of surviving length of expanded flaps. 3. The technique of enhancing tissue expansion with external use of papaverine has the advantages of convenient delivery, cheapness, no more injury and infection, and no side effects etc. It is a safe, effective, simple, and reliable method for accelerating tissue expansion.

Animals ; Papaverine ; administration & dosage ; pharmacokinetics ; Skin ; drug effects ; Surgical Flaps ; Swine ; Swine, Miniature ; Time Factors ; Tissue Expansion ; Vasodilator Agents ; administration & dosage

OBJECTIVETo assess the value of chromosome microarray analysis (CMA) for identifying the etiology of patients with congenital cleft lip and palate.

METHODSTwenty-two patients with no identifiable chromosomal aberrations by conventional cytogenetic technique were selected. DNA was extracted and hybridized with Affymetrix CytoScan(TM) HD arrays following the manufacturer's protocol. The data were analyzed with a CHAS v2.0 software.

RESULTSCMA analysis has identified submicroscopic copy number variants (CNVs) in all of the cases, which have ranged from 100 kb to 1.8 Mb. Potential pathogenic CNVs were identified in 5 patients (22.7%), which involved microdeletions and microduplications on 8p23.1, 10q22.2-q22.3, 6q26, 20p12.1 and 18q12.3. MYST4, MACROD2 and SOX7 genes are likely the causative genes.

CONCLUSIONCMA is an effective method for identification of etiology in patients with cleft lip and palate. CMA should be provided for patients with cleft lip and palate but a normal karyotype. Especially for those with additional structural abnormalities, there is a high risk for submicroscopic chromosomal aberrations.

Child ; Child, Preschool ; Chromosome Aberrations ; Chromosome Disorders ; diagnosis ; genetics ; Cleft Lip ; diagnosis ; genetics ; Cleft Palate ; diagnosis ; genetics ; DNA Copy Number Variations ; Female ; Humans ; Infant ; Male ; Microarray Analysis