Objective: To establish a two-dimensional electrophoresis (2-DE) gel map of 2 early-stage lung adenocarcinoma tissues with and without RASSF1A expression, so as to screen and identify differential proteins. Methods: Five early-stage lung adenocarcinoma tissues with RASSF1A expression and 5 without RASSF1A expression were screened out by Western blotting assay. The total soluble proteins of the tissue were extracted and were separated by immobilized pH gradient based two-dimensional gel electrophoresis to set up the 2-DE gel map of the 2 adenocarcinoma tissues. The differentially-expressed proteins were analyzed by PDQuest image analysis software and identified by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS); the protein database was searched to further characterized the differential proteins. Results: A well-reproducible 2-DE gel map of the 2 adenocarcinoma tissues with and without RASSF1A expression was established and 17 differential protein spots were screened out. Nine of 17 differential protein spots were selected for MALDI-TOF-MS study and satisfactory peptide mass fingerprints were obtained for all the 9 spots. Searching of the protein database revealed 5 candidate proteins and they were: cytochrome b5, 60S acidic ribosomal protein P2, carbonic anhydrase 1, pyrroline-5-carboxylate reductase 1, and apolipoprotein A-I precursor. Conclusion: We have successfully obtained the 2-DE gel images of 2 early-stage lung adenocarcinoma tissues with and without RASSF1A expression, and from which we have identified 5 differential proteins, which paves a way for studying the signal transduction pathways involving RASSF1A.

ObjectiveTo explore effect of family rehabilitation on motor function of stroke patients.MethodsTo provide family rehabilitation with community guide for 106 stroke patients, and observe therapeutic effect.ResultsFamily rehabilitation was effective on 106 cases, and the earlier therapy started, the better effect was.ConclusionFamily rehabilitation is playing an important role in treatment of stroke patients.

Adenocarcinoma ; diagnosis ; genetics ; Carcinoma, Non-Small-Cell Lung ; diagnosis ; genetics ; Carcinoma, Squamous Cell ; diagnosis ; genetics ; Cyclin-Dependent Kinase Inhibitor p16 ; blood ; metabolism ; DNA Methylation ; Genes, p16 ; Humans ; Lung Neoplasms ; diagnosis ; genetics ; Tumor Suppressor Proteins ; blood ; metabolism

Adolescent ; Dermatitis Herpetiformis ; complications ; Esophageal Diseases ; etiology ; Humans ; Male ; Mucous Membrane ; pathology

BackgroundCataract is a leading blinding eye disease,and the prevalence of cataract varies in different regions.ObjectiveThe present study was to investigate the incidence of cataract,surgical coverage rate of cataract and postoperative visual acuity,and to propose a strategy for blindness prevention in Chifeng Keshiketengqi.Methods This is a cross-sectional survey of research.A random cluster sampling method was performed in 12 populated areas in Keshiketengqi.Inhabitants over 50 years in the region were included in this survey.This study was approved by the Ethic Committee of Inner Mongolia People' s Hospital.Informed consents were obtained orally from all the subjects.Disease history,visual acuity,intraocular pressure and regular ophthamological examinations were performed.The inclusion criteria of a pilot study and detailed protocol were employed to calculate the incidence rate of cataract.The questionnaire survey was simultaneously adopted to analyze the cataract-related factors.The surgical coverage rate of cataract in this area was calculated.ResultsIn a total 4234 inhabitants,3826 subjects participated in the survey with a response rate of 90.36％.Cataract was found in 1200 participants with an incidence rate of 31.39％.Age,the female gender and illiteracy were identified as risk factors for cataract (x2 =101.005,P =0.000 ;x2 =58.853,P =0.000 ;x2 =12.037,P =0.007 ).The prevalence rates of cataract were 36.98％ and 33.56％ for illiterates and women,respectively.The incidence rate in the Mongol population was significantly higher than that of the Han population ( 34.83％ vs.29.76％ ) (x2 =9.734,P =0.001 ).The cataract surgery rate increased yearly since 1998.When the vision acuity of the fellow eye was ＜0.1,the surgery coverage rate was 20.60％,but the rates were lower in female,illiterates and the Mongol population (x2 =1.993,P =0.038 ;x2 =16.463,P =0.044 ;x2 =8.523,P =0.029).When a pinhole visual acuity of ＜0.05 was determined as the criteria of blindness,the surgical coverage rate was 28.36％.The cataract surgical coverage rates for Han and Mongolian were 30.67％ and 24.53％,respectively,with a significant difference between them (x2 =12.951,P=0.000).The social burden rate was 3.34％ in this area,with a majority of females,illiterates and the Mongol population ( P＜0.05 ).ConclusionsIn agricultural and pastoral areas of Chifeng Keshiketengqi,the ≥50 years old population has a high rate of cataract blindness and low surgery coverage rate.Surgery is still the preferred way for cataract treatment.

To study the therapeutic effect of Astragalus injection (AI) in treating early stage diabetic nephropathy (DN) patients. Methods: The total of 136 early diabetic nephropathy patients were randomly divided into two groups, 50 cases in the conventional treated group and 86 in the AI treated group, the therapeutic course being 3 weeks. Levels of plasma endothelin-1 (ET-1), 24 hrs urinary albumin excretion rate (uAER), and platelet granule membrane protein (GMP-140), 6-keto-prostaglandin F1α (6-keto-PGF1α), and thromboxane B2 (TXB2) before and after treatment were determined by radioimmunoassay (RIA) and enzyme-linked immunosorbent assay (ELISA) respectively. Moreover, the above-mentioned criteria in 26 healthy subjects were also measured for control. Results: The plasma ET-1, GMP-140, TXB2 and uAER levels in DN patients were higher, but 6-keto-PGF1α level was lower than those in healthy subjects. The above elevated criteria in DN patients could be lowered by AI treatment. Conclusion: The pathogenesis and development of DN might be closely associated with the changes of plasma ET-1 level and platelet function. Astragalus could improve the above-mentioned changes in patients of early stage DN.

In order to develop characteristic folk medicine resources in Jiangxi, a pharmacognostical study was systematically performed for four different origin plants of Sikuaiwa, the result of study provides the microscopic features of powder and tissue of the crude drug. The research provided reference for the identification of Sikuaiwa, as well as a theoretical basis for the further development and the formulation of quality standards.
Magnoliopsida ; anatomy & histology ; chemistry ; growth & development ; Medicine, Traditional ; Plants, Medicinal ; anatomy & histology ; chemistry ; growth & development

Objective To observe whether the changes of electrocardiogram (ECG) is earlier than the morphological changes of ultrasonic cardiogram (UCG) during the development of cardiac pressure over-load left ventricular hypertrophy (LVH) in rabbits, so as to discuss the value of ECG in the early diagnosis of hypertensive LVH. Methods Forty male Japanese rabbits were randomly divided into experimental group (n=25) and control group (n=15). Rabbits in the experimental group were used to establish cardiac pressure over-load LVH model by subtotal ligation of suprarenal abdominal aorta. The control group received the same treatment as that in the experimental group except for the coarctation of abdominal aorta. Before operation and the 2nd, 4th, 6th, and 8th week after operation, 12-lead ECG were recorded to measure R-wave and QRS complex wave amplitude, heart electric axis, QRS duration and QTc period; UCG was performed to detect the thickness of left ventricular inferior wall (LVIW), posterior wall (LVPW) and interventricular septum (IVS), left ventricular end diastolic diameter (LVDd), left ventricular end systolic diameter (LVDs), E/A ratio and left ventricular ejection fraction (LVEF). Before operation and the 8th week after operation, chest X-ray examination was performed to detect cardiothoracic ratio. Then all the rabbits were sacrificed. Left ventricular mass indices (LVMI) were measured and cardiac pathological examination was performed. Results The blood pressure of rabbits increased gradually after operation in the experimental group, the cardiothoracic ratio and LVMI aggrandized, and the cardiomyocytes hypertrophy, denaturalization or necrosis were observed under microscope in the experimental group. The thickness of LVIW, LVPW and IVS of rabbits in the experimental group increased gradually, and the E/A ratio decreased gradually, showing significant difference when compared to those of the control group at the 4th week (P<0.05). LVDd and LVDs of rabbits in the experimental group increased from the 4th week, and were significantly larger than that of the control group after the 6th week (P<0.05). LVEF of rabbits in the experimental group decreased gradually, showing significant difference when compared with the control group at the 8th week (P<0.05). R-wave and QRS complex wave amplitudes in II, III, aVF and chest leads in ECG of the experimental group increased gradually, and became significantly higher than those of the control group at the 2th week (P<0.05). QRS duration and QTc increased gradually after operation, showing significant difference compared to that of the control group at the 4th week (P<0.05). The above mentioned indices had no significant change before or after operation in control group (P>0.05). Conclusion During the development of cardiac pressure over-load LVH of rabbits, the electrical changes of ECG are earlier than the morphologic changes of UCG, which indicates that ECG may has a value for early diagnosis of the hypertensive LVH.

Organic anion transporting polypeptide 1B1 (OATP1B1) is an important liver-specific uptake transporter, which mediates transport of numerous endogenous substances and drugs from blood into hepatocytes. To identify and investigate potential modulators of OATP1B1 from natural products, the effect of 21 frequently used natural compounds and extracts on OATP1B1-mediated fluorescein methotrexate transport was studied by using Chinese hamster ovary cells stably expressing OATP1B1 (CHO-OATP1B1) in 96-well plates. This method could be used for the screening of large compound libraries. Our studies showed that some flavonoids (e.g., quercetin, quercitrin, rutin, chrysanthemum flavonoids and mulberrin) and triterpenoids (e.g., glycyrrhetinic acid and glycyrrhizic acid) were inhibitors of OATP1B1 with IC50 values less than 16 µmol · L(-1). The IC50 value of glycyrrhetinic acid on OATP1B1 was comparable to its blood concentration in clinics, indicating an OATPlB1-mediated drug-drug interaction could occur. Structure-activity relationship analysis showed that flavonoids had much higher inhibitory activity than their glycosides. Furthermore, the type and length of saccharides had a significant effect on their activity. In addition, we used OATP1B1 substrates fluvastatin and rosuvastatin as probe drugs to investigate the substrate-dependent effect of several natural compounds on the function of OATP1B1 in vitro. Our results demonstrated that the effect of these natural products on the function of OATPlB1 was substrate-dependent. In summary, this study would be conducive to predicting and avoiding potential OATP1B1-mediated drug-drug and drug-food interactions and thus provide the experimental basis and guidance for rational drug use.
Animals ; Biological Products ; CHO Cells ; Cricetulus ; Drug Interactions ; Fatty Acids, Monounsaturated ; pharmacology ; Flavonoids ; pharmacology ; Indoles ; pharmacology ; Inhibitory Concentration 50 ; Organic Anion Transporters ; genetics ; metabolism ; Rosuvastatin Calcium ; pharmacology ; Structure-Activity Relationship

AIM: To evaluate the effect of binocular visual function training in pediatric ocular trauma. METHODS: There were 76 patients (76 eyes) that were hospitalized with a primary diagnosis of ocular injury at the Affiliated Hospital of Medical College, Qingdao University between January 2006 and December 2009. Binocular visual function training was given after primary wound repair. Far stereopsis function were checked using AIT-1000 synoptophore fusion, and near stereopsis function was checked using Titmus stereogram.Binocular visual function was compared before and after training. RESULTS: Before binocular visual function training,26 eyes（34%）had no binocular vision, after training there were only 16 eyes（21%） without binocular vision. Before undertaking binocular visual function training with fusion, only 27 eyes (36%) had binocular vision, after the training there were 48 eyes (63%) with binocular vision. Before undertaking binocular visual function training with far stereopsis ,there were 23 eyes (30%) with binocular vision, after the training there were 29 eyes (38%). Before binocular visual function training with near stereopsis, there were 14 eyes (18%) with binocular vision, after the training there were 33 eyes (43%) with binocular vision. There was a significant difference in the number of patients with binocular vision before and after binocular visual function training. CONCLUSION:The training is useful for the reconstruction of binocular visual function in pediatric ocular trauma.