Glycyrrhiza uralensis Fish Polysacchoride(GPS)was observed to have mitogenic activity on murine spleen cell proliferation in vitro,The results showed that this mitogenic activity of GPS may be directed to B Iymphocytes,i.e.GPS is a new mitogen of murine B lymphocytes.Furthermore,it was found that B lymphocytes stimulated by GPS was M? depedent and T cell-indepedent.On the contrary,antibodies and IL-2 production was inhibited by GPS in vivo.

Rat with amputation operation stress possessed a decline of NK activity and splenocyte pro-liferation to mitogen ConA.Amygdaloid nuclei lesion could antagonize the decrease of those ofcell-mediated immunity.Further results showed that serum corticosterone and methionine-enkephahn was high in stress' rats.Amugdaloid Nuclei lesion could antagonize the increase ofserum corticosterone level,but seem no dffeet on serum methionine-enkephalin level,thereforesuggesting that it is related to ratio of corticosterone to enkephalin changed by decrease of corti-costerone that amygdaloid neuclei lesion antagonized the decline of immunogical function inducedby stress.

AlM: To understand the prognostic factors of myopic rhegmatogenous retinal detachment ( RRD) surgery, and provide guidance for clinical treatment.
METHODS: This retrospective study included 288 myopic RRD eyes ( excluding trauma, aphakia, congenital diseases, tractional and exudative retinal detachment ) from April 2007 to April 2014 in our department. The surgical anatomic reduction situation was statistically recorded, the effects of hole's size, retinal detachment duration on sight restoration and surgery success rate were analyzed
RESULTS:The statistical results showed that 245 eyes (85. 1%) healed after their first operation, about 95. 1%cases finally healed after their second or third operation. The degree of myopia and aging factor influenced the RRD operation:the higher degree and the older age were, the lower success rates were (P<0. 05). Through surgery, postoperative visual acuity was significantly better than the preoperative ones ( P<0. 01 ). A higher degree of myopia RRD led to poor visual acuity ( P < 0. 01 ). Postoperative visual acuity of 152 eyes ( 52. 9%) were improved, of which 106 eyes' visual acuity was 0. 05 or more.
CONCLUSlON:Surgery is an effective means of treating myopic RRD. The older patients have lower success rates. A higher degree of myopia led lower reset rate after retinal detachment surgery and poor postoperative visual acuity. When treat myopic RRD, we should pay attention to the recovery of visual function.

Objective To observe the time interval changes of the expression of Nogo-A in the intumescentia lumbalis of rats with experimental allergic encephalomyelitis(EAE),and explore the function of Nogo-A in the happening and development of EAE.Methods One hundred and six Wistar rats(female) were randomly divided into 2 groups:EAE group(70 rats)and control group(36 rats).Female Wistar rats were used to make EAE animal models,immunized by fresh cavia cobaya spinal cord homogenate and complete Freund adjuvant(CFA),at the same time,their blood brain barrier permeability was lifted by using pertussis stock solution.The rats those were coincident with the standard of being picked up were divided into 6 groups randomly according to the time of falling ill.In control group,fresh guinea pig spinal cord homogenate was replaced by sodium chloride,and rats were also divided into 6 groups randomly.Immunohistochemistry SP was used to detect expression of Nogo-A.Amyelination was detected by improved acid fuchsin and azulin myelin staining.Results In the control group,differences of Nogo-A expression in the intumescentia lumbalis were not obvious in each time group.In the EAE group,expression of Nogo-A could be observed in each time group,but their quantities were very different in contrast with those in the control group.One day after the rats had clinic symptoms,the expression of Nogo-A decreased to some extent;the third day,it reached the lowest point,and was significantly lower than that in control group(P

Objective: To observe the effect of scraping therapy (ST) on the immune balance of serum helper T (Th) 1/Th2 cells in autologous nucleus pulposus transplantation non-compressive lumbar disc herniation (LDH) model rats, and explore the immune mechanism of ST in reducing LDH pain. Methods: Seventy-two male Sprague-Dawley (SD) rats were randomly divided into a model group, a sham operation group and a ST group, with 24 rats in each group. Autologous nucleus pulposus transplantation non-compressive LDH models were established in the model group and ST group, while the rats in the sham operation group underwent sham operation without model establishment. On the fifth day after the model was successfully prepared, rats in the ST group received ST, once every other day, 3 times as a course for a total of 3 courses. Six rats in each group were randomly selected to observe their pain thresholds, and peripheral blood of the rats was collected before the first scraping treatment and at the end of the first, second, and third courses of treatment. The serum was isolated and enzyme-linked immunosorbent assay (ELISA) was applied for the detection of rat serum interferon (IFN)-γ, interleukin (IL)-4 and IL-10. Results: Compared with the model group, the pain threshold in LDH rats in the ST group increased (P<0.05), the serum IFN-γ level was significantly reduced (P<0.05), but the levels of IL-4 and IL-10 did not change significantly (both P>0.05). At the end of the second and third courses of treatment, the IFN-γ/IL-4 ratio was negatively correlated with the pain threshold in the rats, and the IFN-γ/IL-4 ratio was significantly reduced in the ST group (P<0.01). Conclusion: ST can help suppress the Th1 immunity in LDH rats triggered by the autologous nucleus pulposus, restore the immune balance of Th1/Th2, and reduce the pain of LDH.

The systemic anaphylaxis murine model was induced by the injection of oval albumin (OVA)in aluminum hydroxide gel adjuvant. The death rate varied with the mouse species: 40-90% in Swiss and 69% in average,no death in C57BL/6 and 615 but some symptoms. developed. Swiss mice was adopted in this experiment.90% of mice pretreated with Lx remained living after allergen challenge,but only 20% in normal control. For elucidation the possible mechanism of Lx action,the study was carried out on detection of anti-OVA IgE specific antibody and histamin concentration in lung tissues. Passive cutaneous anaphylaxis(PCA)titers for anti-OVA IgE antibody in serum ranged from undilution to 160 in control group and not detectable in group pretreated with Lx. In addition, after absorption of PCA positive sera with anti-IgG antibody.the PCA activity was not affected. Histamin content was 1.54?0.24?g/ml in Lx treated mice caopared to 2.69?0.67?g/ml in control group(p

The levels of c-fos mRNA in U937 cell line were determined with in situ hybridization.Theresults showed that TPA induced the expression of c-fos gene with a relationship of dosage-ef-fect.It was also proved that GM-CSF,TNF,IL-9,IL-3 and IL-6 increased the expression of c-fos gene.

Adult ; Aged ; Female ; Humans ; Lymphoma ; pathology ; Male ; Middle Aged ; Retrospective Studies ; Splenic Neoplasms ; pathology

Objective To investigate the relationship between the prothrombotic state and leukoaraiosis(LA)in elderly patients with metabolic syndrome.Methods A total of 350 metabolic syndrome patients in the affiliated hospital of Zhejiang University who received treatment from January 2013 to January 2015.According to the results of head MRI,patients were divided into LA group(n=203)and non-LA group(n=147).The severity of LA was graded by its MRI appearance and the Blennow scale.The plasma levels of fibrinogen (Fib),prothrombin time (PT),thrombin time (TT),activated partial blood coagulation time(APTT),D-dimer (D-D) and yon willebrand factor (vWF) were measured and compared between the two groups.Results The levels of Fib,vWF and D-D were higher in LA group than in non-LA group(all P＜0.05).Spearman correlation analysis showed that the severity of leukoaraiosis was positively correlated with Fib,vWF and D-D(r=0.014,0.089,0.215,respectively,all P ＜ 0.05).Multivariate linear regression analysis showed that Fib,course of hypertension,vWF and HbA1c were the main influencing factors with the standardized regression coefficient of 2.516,0.312,0.206 and 0.167 respectively for leukoaraiosis in elderly patients with metabolic syndrome.Conclusions The course of hypertension and HbAlc level are positively related with LA.The prothrombotic state markers including Fib and vWF can be considered as the predictors for LA severity in elderly patients with metabolic syndrome.