1.Difference of the migration ability between PTEN transfected breast cells and non-transfected cells
Guanping LIN ; Jinwen HUANG ; Keyuan ZHOU
Cancer Research and Clinic 1999;0(05):-
Objective To disclose the influence of PTEN on the migration of breast cancer cells ZR-75-1. Methods Wild-type PTEN gene was transtected into ZR-75-1 breast cancer cells which lack PTEN gene, tranfected cells were selected by puromycin and the protein encoded by PTEN gene was tested by Western-blot. The inhibition rate of invasion and adhesion of ZR-75-1 was tested on reconstituted basement membrane. Results Wild-type PTEN gene was successfully transfected into ZR-75-1 and expressed efficiently. The inhibition rate of invasion and adhesion is 70.4 % and 60.0 % respectively. Conclusion PTEN gene can restrict the migration of breast cancer cells in some degree, so whether PTEN gene is deleted or not can partly estimates the risk of migration of breast cancer cells.
2.Chronic prostatitis following sexually transmitted urethritis(report of 86 cases)
Weiping HONG ; Guanping LIN ; Shuiyuan KE
Chinese Journal of Urology 2000;0(05):-
Objective To investigate the etiologic factors of chronic prostatitis following sexually transmitted urethritis. Methods The pathogens have been detected in 86 patients with chronic prostatitis following sexually transmitted urethritis. Results 57 strains (66.3%) of bacteria were cultured in expressed prostatic secretions(EPS) from 86 patients,in which gonococcus was the majority of cultures,accounting for 38.4%. Chlamydia trachomatis (CT), ureaplasma urialyticum (UU) and gonococcus were also detected by polymerase chain reaction (PCR) in expressed prostatic secretions,25.6%(22/86), 22.0%(19/86),and 43.0%(37/86) showed positive results respectively. Conclusions Gonococcus,chlamydia trachomatis and ureaplasma urialyticum were the main pathogens of chronic prostatitis.The results indicate that pathogen examination plays an important role in treatment of chronic prostatitis following sexually transmitted urethritis.
3.Congenital lipoid adrenal hyperplasia with 46, XY karyotype and male external genitalia: Two cases and literature review
Xinyi LIANG ; Wei WU ; Ke HUANG ; Xuefeng CHEN ; Jinna YUAN ; Hu LIN ; Guanping DONG
Chinese Journal of Endocrinology and Metabolism 2021;37(9):836-839
Congenital lipoid adrenal hyperplasia (CLAH) is a rare autosomal recessive disorder, which is characterized by adrenal insufficiency and 46, XY sex reversal. Two cases of CLAH with 46, XY karyotype exhibited male external genitalia were reported to explore the clinical and genetic features. A retrospective analysis of CLAH with relevant literatures was performed.
4.Factors influencing medical narrative competence and its correlation with psychological resilience in pediatric staff
Xuelian ZHOU ; Ke HUANG ; Hu LIN ; Li ZHANG ; Zhaoyuan WU ; Yuanyuan MENG ; Wei WU ; Guanping DONG ; Junjun JIA ; Junfen FU
Chinese Journal of Medical Education Research 2024;23(3):321-326
Objective:To investigate the medical narrative competence of pediatric staff, and analyze its influencing factors and correlation with psychological resilience, and to discuss strategies to improve narrative competence.Methods:From January 11 to February 25, 2022, by convenience sampling, we sampled pediatric personnel and those on refresher training at Children's Hospital, Zhejiang University School of Medicine for a questionnaire survey involving general information, the narrative competence scale, and the 14-item resilience scale. With the use of SPSS 26.0, the narrative competence of different populations was compared, and factors affecting narrative competence were determined through Pearson correlation analysis and multiple regression analysis.Results:A total of 361 valid questionnaires were included in this study, and there was significant differences in the narrative competence score between different ages, professional titles, working years, income levels, and whether they wrote parallel charts ( P<0.05). The total score of narrative competence of pediatric staffs was (147.13±18.76), and positively correlated with the total resilience score and the score of each dimension ( P≤0.001). The regression analysis showed that writing parallel charts and resilience could explain 53.10% of the variation in narrative competence ( P<0.001). Conclusions:Pediatric staff's narrative competence is at low or intermediate levels. Parallel chart writing and resilience training can improve narrative competence and promote a harmonious doctor-patient relationship.