1.The effects of 2-hydroxyethyl methacrylate on the proliferation and migration of human dental pulp cells
Su SUN ; Ming FANG ; Lijuan SHEN ; Guanlin WANG ; Hailang DIWU ; Yuhong XIAO
Journal of Practical Stomatology 2015;(5):648-652
Objective:To assess the effects of HEMA on the proliferation and migration of human dental pulp cells(hDPCs).Meth-ods:hDPCs were obtained using tissue explant culture technique in vitro,and then cells of the 3rd -5th passages were treated by differ-ent concentrations of HEMA for 24,48 and 72 h respectively.Cell proliferation was examined by MTT assay.Cell migration was ob-served by Transwell method.Results:The proliferation ability of hDPCs decreased when exposed to HEMA in both time and concentra-tion dependent manner(vs control,P <0.05).Cell proliferation at 24 h expossure was statistically higher than that at 48 h and 72 h(P<0.05).The migration of hDPCs was significantly reduced in HEMA groups at different concentrations(vs control,P <0.05).Con-clusion:HEMA inhibits the proliferation and migration of human dental pulp cells in vitro.
2.Effects of As(2)O(3) on BCR-ABL protein level and signal transduction in CML cells.
Zhongbin LAI ; Guanlin SUN ; Weili WU ; Yingli WU
Chinese Journal of Hematology 2002;23(7):360-362
OBJECTIVETo explore the effects of As(2)O(3) on BCR-ABL protein level and signal transduction in chronic myeloid leukemia (CML) cells.
METHODSImmunoprecipitation, protein tyrosine kinase (PTK) activity assay, real-time Taqman quantitative PCR and Western blot were used.
RESULTSAs(2)O(3) downregulated BCR-ABL protein and STAT1 protein of CML mononuclear cells in the concentrations of 1.0 approximately 2.0 micro mol/L and 0.5 approximately 2.0 micro mol/L after 48 h exposure, respectively. However, p27 protein level was not affected. The PTK activity of BCR-ABL protein was also mildly decreased in CML monouclear cells at 60 h. The bcr-abl mRNA level remained unchanged.
CONCLUSIONAs(2)O(3) downregulats BCR-ABL protein, STAT1 protein, BCR-ABL signal transduction and PTK activity in CML cells.
Fusion Proteins, bcr-abl ; genetics ; Humans ; K562 Cells ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive ; genetics ; Phosphorylation ; drug effects ; Protein-Tyrosine Kinases ; metabolism ; Signal Transduction
3.Non-invasive biomarkers for liver inflammation in non-alcoholic fatty liver disease: present andfuture
Terry Cheuk-Fung YIP ; Fei LYU ; Huapeng LIN ; Guanlin LI ; Pong-Chi YUEN ; Vincent Wai-Sun WONG ; Grace Lai-Hung WONG
Clinical and Molecular Hepatology 2023;29(Suppl):S171-S183
Inflammation is the key driver of liver fibrosis progression in non-alcoholic fatty liver disease (NAFLD). Unfortunately, it is often challenging to assess inflammation in NAFLD due to its dynamic nature and poor correlation with liver biochemical markers. Liver histology keeps its role as the standard tool, yet it is well-known for substantial sampling, intraobserver, and interobserver variability. Serum proinflammatory cytokines and apoptotic markers, namely cytokeratin-18, are well-studied with reasonable accuracy, whereas serum metabolomics and lipidomics have been adopted in some commercially available diagnostic models. Ultrasound and computed tomography imaging techniques are attractive due to their wide availability; yet their accuracies may not be comparable with magnetic resonance imaging-based tools. Machine learning and deep learning models, be they supervised or unsupervised learning, are promising tools to identify various subtypes of NAFLD, including those with dominating liver inflammation, contributing to sustainable care pathways for NAFLD.
4.Preliminary Study on the Effects of Novel Retinoids SX-115 and CHU-012 on NB4 Cell Line
Yiyun YAO ; ; Guanlin SUN ; Zongru GUO ; Weili WU ; Yun WANG ; Hui SU
Journal of Experimental Hematology 2000;8(3):185-188
In this research, the effects of two new retinoids, SX-115 and CHU-012, on promyelocytic leukemia cell line NB4 were studied in vitro. Cell proliferation, cell morphologic characters, cell cycle kinetics, reduction ability of NBT, differentiation antigens, immunofluorescence staining and RT-PCR were adopted as the observational parameters. The results showed that SX-115 and CHU-012 induced differentiation of NB4 cells at concentration of 10(-6) mol/L. Comparing the effects of the two retinoids with all trans-retinoic acid (ATRA) at same concentration, there was no significant difference among the three agents. The mechanism of the 2 new retinoids remains possibly the same as ATRA.
5.Non-invasive tests of non-alcoholic fatty liver disease.
Guanlin LI ; Xinrong ZHANG ; Huapeng LIN ; Lilian Yan LIANG ; Grace Lai-Hung WONG ; Vincent Wai-Sun WONG
Chinese Medical Journal 2022;135(5):532-546
For the detection of steatosis, quantitative ultrasound imaging techniques have achieved great progress in past years. Magnetic resonance imaging proton density fat fraction is currently the most accurate test to detect hepatic steatosis. Some blood biomarkers correlate with non-alcoholic steatohepatitis, but the accuracy is modest. Regarding liver fibrosis, liver stiffness measurement by transient elastography (TE) has high accuracy and is widely used across the world. Magnetic resonance elastography is marginally better than TE but is limited by its cost and availability. Several blood biomarkers of fibrosis have been used in clinical trials and hold promise for selecting patients for treatment and monitoring treatment response. This article reviews new developments in the non-invasive assessment of non-alcoholic fatty liver disease (NAFLD). Accumulating evidence suggests that various non-invasive tests can be used to diagnose NAFLD, assess its severity, and predict the prognosis. Further studies are needed to determine the role of the tests as monitoring tools. We cannot overemphasize the importance of context in selecting appropriate tests.
Elasticity Imaging Techniques/methods*
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Humans
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Liver/pathology*
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Liver Cirrhosis/pathology*
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Magnetic Resonance Imaging/methods*
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Non-alcoholic Fatty Liver Disease
6.Novel approach for real-time monitoring of carrier-based DPIs delivery process pulmonary route based on modular modified Sympatec HELOS.
Xuejuan ZHANG ; Yingtong CUI ; Ruifeng LIANG ; Guanlin WANG ; Xiao YUE ; Ziyu ZHAO ; Zhengwei HUANG ; Ying HUANG ; Jianfang GENG ; Xin PAN ; Chuanbin WU
Acta Pharmaceutica Sinica B 2020;10(7):1331-1346
An explicit illustration of pulmonary delivery processes (PDPs) was a prerequisite for the formulation design and optimization of carrier-based DPIs. However, the current evaluation approaches for DPIs could not provide precise investigation of each PDP separately, or the approaches merely used a simplified and idealized model. In the present study, a novel modular modified Sympatec HELOS (MMSH) was developed to fully investigate the mechanism of each PDP separately in real-time. An inhaler device, artificial throat and pre-separator were separately integrated with a Sympatec HELOS. The dispersion and fluidization, transportation, detachment and deposition processes of pulmonary delivery for model DPIs were explored under different flow rates. Moreover, time-sliced measurements were used to monitor the PDPs in real-time. The Next Generation Impactor (NGI) was applied to determine the aerosolization performance of the model DPIs. The release profiles of the drug particles, drug aggregations and carriers were obtained by MMSH in real-time. Each PDP of the DPIs was analyzed in detail. Moreover, a positive correlation was established between the total release amount of drug particles and the fine particle fraction (FPF) values ( = 0.9898). The innovative MMSH was successfully developed and was capable of illustrating the PDPs and the mechanism of carrier-based DPIs, providing a theoretical basis for the design and optimization of carrier-based DPIs.
7. Effects of ω-3 polyunsaturated fatty acids from different sources on glucolipid metabolism in type 2 diabetic patients with dyslipidemia
Feng WANG ; Hechun LIU ; Xiaosong LIU ; Shunan DONG ; Da PAN ; Ligang YANG ; Guiju SUN
Chinese Journal of Preventive Medicine 2019;53(6):570-575
Objective:
To determine the effects of ω-3 polyunsaturated fatty acids from different sources on glucolipid metabolism in type 2 diabetic patients with dyslipidemia.
Methods:
We recruited participants from the diabetes specialist clinic at the Guanlin hospital in Yixing city, Jiangsu Province from February 2017 to March 2017. A total of 180 subjects were randomly assigned to 3 g/day fish oil (FO), perilla oil (PO), or fish oil mixed with linseed oil (FLO) for 6 months. The basic conditions and fasting venous blood sample were obtained from each study subject at baseline, after 6 months of intervention. Serum glucose and lipid metabolism were investigated.
Results:
A total of 156 subjects aged (62.6±8.6) years completed the final follow-up after 6 months (FO,54 subjects; PO,52 subjects; FLO,50 subjects). Among them,59 patients (37.8%) were male. Serum glucose, glycated hemoglobin, C peptide, insulin and homeostasis model assessment-insulin resistance were not significantly different among the three groups after 6 months. Serum triglyceride decreased, whereas high-density lipoprotein cholesterol increased in FO [1.33 (1.05,1.93) mmol/L, (1.36±0.29) mmol/L, respectively] compared with PO [1.71 (1.23, 2.17) mmol/L, (1.23±0.22) mmol/L, respectively] and FLO [1.51 (1.12, 2.22) mmol/L, (1.29±0.30) mmol/L, respectively] (
8.A real-time and modular approach for quick detection and mechanism exploration of DPIs with different carrier particle sizes.
Yingtong CUI ; Ying HUANG ; Xuejuan ZHANG ; Xiangyun LU ; Jun XUE ; Guanlin WANG ; Ping HU ; Xiao YUE ; Ziyu ZHAO ; Xin PAN ; Chuanbin WU
Acta Pharmaceutica Sinica B 2022;12(1):437-450
Dry powder inhalers (DPIs) had been widely used in lung diseases on account of direct pulmonary delivery, good drug stability and satisfactory patient compliance. However, an indistinct understanding of pulmonary delivery processes (PDPs) hindered the development of DPIs. Most current evaluation methods explored the PDPs with over-simplified models, leading to uncompleted investigations of the whole or partial PDPs. In the present research, an innovative modular process analysis platform (MPAP) was applied to investigate the detailed mechanisms of each PDP of DPIs with different carrier particle sizes (CPS). The MPAP was composed of a laser particle size analyzer, an inhaler device, an artificial throat and a pre-separator, to investigate the fluidization and dispersion, transportation, detachment and deposition process of DPIs. The release profiles of drug, drug aggregation and carrier were monitored in real-time. The influence of CPS on PDPs and corresponding mechanisms were explored. The powder properties of the carriers were investigated by the optical profiler and Freeman Technology four powder rheometer. The next generation impactor was employed to explore the aerosolization performance of DPIs. The novel MPAP was successfully applied in exploring the comprehensive mechanism of PDPs, which had enormous potential to be used to investigate and develop DPIs.
9.Association between wrist pain and awkward postures among workers in 10 key industries
Guanlin LI ; Xin SUN ; Meibian ZHANG ; Huadong ZHANG ; Ruijie LING ; Yimin LIU ; Gang LI ; Nengzhou CHEN ; Zaoliang REN ; Yan YIN ; Hua SHAO ; Hengdong ZHANG ; Jiajie LI ; Bing QIU ; Dayu WANG ; Qiang ZENG ; Zhanhui LIANG ; Rugang WANG ; Jianchao CHEN ; Danying ZHANG ; Liangying MEI ; Yongquan LIU ; Jixiang LIU ; Chengyun ZHANG ; Tianlai LI ; Ning JIA ; Junyi WANG ; Zhongxu WANG ; Qingsong CHEN
Journal of Environmental and Occupational Medicine 2023;40(1):49-54
Background Prolonged awkward postures during occupational activities can lead to excessive musculoskeletal load on the wrist of workers and symptoms such as wrist pain or discomfort. Objective To survey the prevalence of wrist pain among workers in 10 key industries and analyze its correlation with wrist working postures. Methods By using stratified cluster sampling method, workers from 10 key industries, such as footwear manufacturing industry, shipbuilding manufacturing industry, and automobile manufacturing industry, were selected from seven regions in North China, East China, Central China, South China, Southwest China, Northwest China, and Northeast China. The demographic information, wrist working postures, pain in wrist of the workers were collected through a cross-sectional survey. Pearson χ2 test was used to compare prevalence by selected factors, trend χ2 test for between group comparison, and unconditional logistic regression models for the association of wrist working postures with wrist pain. Results There were 64052 workers enrolled in this survey, and 56286 provided valid questionnaires (the effective rate was 87.8%). According to the survey, the prevalence of wrist pain was 23.3% (13112/56286), and the industries with higher prevalences were footwear manufacturing (27.1%, 1927/7106), automobile manufacturing (24.9%, 5378/21560), and shipbuilding and related equipment manufacturing (24.4%, 850/3488) industries. Finger pinching (OR=2.09, 95%CI: 1.95-2.24), frequent wrist bending (OR=2.03, 95%CI: 1.92-2.15), fixed wrist bending (OR=1.77, 95%CI: 1.69-1.85), wrist on hard edge (OR=1.34, 95%CI: 1.28-1.40), and arms over shoulders (OR=1.11, 95%CI: 1.05-1.17) increased the risk of reporting wrist pain. Conclusion Awkward postures are related to wrist pain among workers in selected 10 key industries. The related factors are wrist on hard edge, frequent wrist bending, finger pinching, fixed wrist bending, and arms over shoulders.