In this paper, 17 autopsy cases of poisoning by toxic plants: mushroom 4, xanthium sibiricum 3, trichosanthes kirilowii 2, aconitum chinense 3, tripterygium wilfordii 1, gelsemium elegans 1, nerium indicum 1, pachyrhizus erosus 1, dioscorea simulans 1 were reported (accident 15, suicide 2). Emphasis was put on analyses of the selective injured locations, namely, target organs or tissues, which were poisoned by these poisonous plants. The machanisms of poisoning and causes of death were approached based on the pathological changes. The associated problems of forensic medicine were discussed summarily. On the basis of author's experiences, it is essential for medicolegal examination and expertise that the body should be systematically, completely, forensic-pathologically inspected and the species of questioned poisonous plants should be identified by the associated expert. Poisoning by toxic plants is one of often contacted and difficult problems in medicolegal expertise, so that we should pay attention to it.

Objective To study the surgical managements for obstructive coleratal carcinoma(OCRC).(Methods) Clinical data of 126 patients with OCRC who underwent surgical treatment between January 1995 and December 2004 were reviewed.Results Thirty-five patients received one-stage right hemicolectomy,ten patients received one-stage resection of transverse colon.forty-eight patients received one-stage left(hemicolectomy),eighteen patients received one-stage resection of left colon or proximal rectum with colostomy followed by two-staged anastomosis.For 15 patients with unresectable lesions,colostomy of transverse colon or sigmoid colon was performed.Seventeen patients(13.5%) experienced postoperative complications.The (operative) mortality was 4.8%(6/126).The 1-year,3-year and 5-year overall survival rate was(95.1)%,52.7% and 38.1%,respectively.Conclusions Proper perioperative period managements and(selection) of surgical techniques are important for better results and lower complications in the treatment OCRC.

Carcinoma, Squamous Cell ; Humans ; Mouth Neoplasms ; NF-kappa B ; Neoplasm Proteins

This paper reports the results of forensic pathological study of 128 autopsycases of sudden coronary death(SCD).The severity of the coronary arterystenosis was 4 degree in 63 cases,3 degree in 26 cases and 2 degree in 39 cases.The distribution of the artheroscleorosis of 3 and 4 degree was quite extensive. Recent thrombosis in CA was found in 18 cases,hemorrhage in plaques in 17cases.Only 2 cases had acute myocardial infarction.Inflammatory cell infil-tration were found in coronary plaques in 36 cases.Myocardail fibrosis orsmall scar formation were detected in 56 cases.It is suggested that SCD isthe commonest cause of sudden unexpected death.The majority of SCD(61%)were manhood in middle age.Most cases died suddenly during sleep withoutany clear inducements.The characteristics of the pathological changes in theCA and myoc ardium and the pathological diagnosis of SCD were analyzedand discussed.

Myoglobin (Mb)depletion from myocardium in the cases of sudden soronary death (SCD) and was firstly studied by an immunhistochemial technique (ABC method) in China. Mb was detected quantitatively using scanning microscope photmeter and the results were analyzed statistically by computer The results showed that there were marked depletion of Mb from myocardium in each case of SCD The Mb depleted arce were multiple, disseminatly and segmentally distributed while no depletion of Mb from myocardium in the cases of control group was seen We suggested that the depletion of Mb can be used as one of the diagnostic criterion in the cases of SCD.

Objective To observe the pathological changes of major organs in rats with acute Dysosma versipellis poisoning and investigate the toxic mechanismand the injuries of target tissues and organs. Methods Forty Sprague-Daw ley (SD) rats were random ly divided into three experimental groups, which were given the gavage with 0.5, 1.0 and 2.0LD50 doses of Dysosma versipellis decoction, and one con-trol group, which was given the gavage with 1.0LD50 dose of normal saline. The rats were sacrificed 14 days after Dysosma versipellis poisoning and sam ples including brain, heart, liver, lung, and kidney were taken. After pathological process, the pathological changes of the major organs and tissues were observed by light microscope and electron microscope. The experimental data were statistical analyzed by x2 test. Results The observations of light microscopy: loose cytoplasmof neurons with loss of most Nissl bodies;swelling of m yocardial cells with disappearance of intercalated disk and striations;hepato-cellular edema with ballooning degeneration; and swelling epithelial cells of renal proximal convoluted tubule with red light coloring protein-like substances in the tube. The observations of electron microscopy:the structures of cell mem brane and nuclear mem brane of neurons were destroyed;cytoplasmof neurons, obvious edema;and most organelles, destroyed and disappeared. The mortalities of rats after acute poi-soning of the four groups increased with doses (P<0.05). Conclusion Acute Dysosma versipellis poisoning can cause multi-organ pathological changes. There is apositive correlation between the toxic effect and the dosage. The target tissues and organs are brain (neurons), heart, liver and kidney.

Objective To explore the feasibility of in vitro biological soft tissue imaging by using synchrotron radiation phase contrast CT.Methods Three samples of resected human cardia,two samples of resected human esophageal carcinoma and esophagus,as well as two samples of middle cerebral artery tissue extracted from corpses were fixed and airdried at room temperature for synchrotron radiation phase contrast CT imaging.The images of soft tissue structures were observed and compared with pathological findings.Results The images of synchrotron radiation phase contrast CT showed three-layer structure of cardia and esophagus,mucous,submucosa and muscular layer.The surface of mucous layer was smooth.The images of esophageal carcinoma showed cancerous tissue infiltrating esophageal wall.The wall and lumen of cerebral arteries could be also clearly displayed.Conclusion Synchrotron radiation phase contrast CT imaging can clearly display fine structures of in vitro biological soft tissue.

Objective: To investigate the changes of microorganisms and metabolites in joint effusion of patients with Rheumatoid arthritis(RA), Osteoarthritis(OA) and Urarthritis(UA). To provide new ideas for the study of the effect of microbiota on the pathogenesis of arthritis. Methods: Joint effusion samples were collected from 20 patients with RA, 20 patients with OA, and 20 patients with UA. 16S rRNA gene sequencing and untargeted ultra-high performance Liquid chromatography-mass spectrometry(LC-MS) were used to explore the differences in microorganisms and metabolites among the three groups. Pearson correlation analysis was used to detect the correlation between effusion microbiota and metabolites. Results: There were differences in microbial diversity and microbiota composition among the three groups. Combined with VIP>1 from OPLS-DA andP<0.05 from two-tailed Students t-test, 45 differential metabolites(Between RA and OA groups), 38 differential metabolites(Between UA and OA groups) and 16 differential metabolites(Between RA and UA groups), were identified. GO analysis and KEGG pathway analysis showed that the differential metabolic pathways among the three groups were mainly concentrated in citric acid cycle(TCA cycle), nucleotide metabolism, amino acid metabolism and glycolysis pathway. Correlation analysis of joint effusion microbiota and metabolites suggested that bacteria enriched in the three groups of joint effusion, such asPrevotella,Clostridium ruminosus,Prevotellaceae＿UCG-001, were related to many key metabolites such as lysozyme, uric acid, glucose, and L-glutamine. Conclusion This study shows that there are a variety of bacterial flora in joint cavity effusion of RA, OA, and UA patients, and the differential metabolites produced by them are involved in the pathogenesis of the three types of arthritis by affecting a variety of metabolic pathways.